protein coding gene - SPAC4G8.09 - mitochondrial leucine-tRNA ligase

Gene summary

Systematic ID: SPAC4G8.09
Product: mitochondrial leucine-tRNA ligase
Organism: Schizosaccharomyces pombe (fission yeast)
UniProt ID: Q09828
ORFeome ID: 38/38F10
Characterisation status: biological role inferred
Feature type: mRNA gene
Genomic location: chromosome I: 778400..781324 forward strand

Annotation

Disease association

MONDO:0014869 - hydrops-lactic acidosis-sideroblastic anemia-multisystemic failure syndrome

References:

PB_REF:0000006

MONDO:0014126 - Perrault syndrome 4

References:

PB_REF:0000006

GO biological process

GO:0006429 - leucyl-tRNA aminoacylation

References:

GO_REF:0000033

GO:0032543 - mitochondrial translation

References:

GO_REF:0000033

GO cellular component

GO:0005759 - mitochondrial matrix

References:

GO_REF:0000111

GO:0005739 - mitochondrion

References:

PMID:16823372

GO molecular function

GO:0002161 - aminoacyl-tRNA deacylase activity

References:

GO_REF:0000002

GO:0005524 - ATP binding

References:

GO_REF:0000002

GO:0004823 - leucine-tRNA ligase activity

References:

GO_REF:0000033

GO:0003729 - mRNA binding

References:

GO_REF:0000024

Protein sequence feature

SO:0001808 - mitochondrial_targeting_signal

References:

PMID:36408920

Quantitative gene expression

PBO:0006310 - protein level

References:

PBO:0011963 - RNA level

References:

PMID:23101633

Single locus phenotype

FYPO:0002111 - inviable tapered vegetative cell

References:

PMID:23697806

Genotypes:

SPAC4G8.09delta

FYPO:0002061 - inviable vegetative cell population

References:

Genotypes:

SPAC4G8.09delta

FYPO:0000750 - sensitive to silver ions

References:

PMID:29294138

Genotypes:

SPAC4G8.09+(wild type)/SPAC4G8.09delta

FYPO:0006929 - sensitive to silver nanoparticles

References:

PMID:29294138

Genotypes:

SPAC4G8.09+(wild type)/SPAC4G8.09delta

Taxonomic conservation

PBO:0011067 - conserved in bacteria

PBO:0011065 - conserved in eukaryotes

PBO:0011064 - conserved in fungi

PBO:0011069 - conserved in metazoa

PBO:0011070 - conserved in vertebrates

PBO:0006222 - predominantly single copy (one to one)

Protein features

ID	Name	InterPro name	DB name
PF08264	Anticodon_1	M/V/L/I-tRNA-synth_anticd-bd	PFAM
PF09334	tRNA-synt_1g	Methionyl/Leucyl_tRNA_Synth	PFAM
PF00133	tRNA-synt_1	aa-tRNA-synth_Ia	PFAM
PF13603	tRNA-synt_1_2	Leu_tRNA-synth_edit	PFAM
cd00812	LeuRS_core		CDD
PS00178	AA_TRNA_LIGASE_I	aa-tRNA-synth_I_CS	PROSITE_PATTERNS
PR00985	TRNASYNTHLEU	Leu-tRNA-ligase	PRINTS
G3DSA:3.40.50.620:FF:000100			FUNFAM
G3DSA:1.10.730.10:FF:000002			FUNFAM
G3DSA:3.40.50.620:FF:000003			FUNFAM
SSF52374	Nucleotidylyl transferase		SUPERFAMILY
SSF47323	Anticodon-binding domain of a subclass of class I aminoacyl-tRNA synthetases	tRNAsynth_Ia_anticodon-bd	SUPERFAMILY
SSF50677	ValRS/IleRS/LeuRS editing domain	Val/Leu/Ile-tRNA-synth_edit	SUPERFAMILY
G3DSA:3.40.50.620	HUPs	Rossmann-like_a/b/a_fold	GENE3D
G3DSA:1.10.730.10			GENE3D
PTHR43740	LEUCYL-TRNA SYNTHETASE	Leu-tRNA-ligase	PANTHER
MF_00049_B	Leu_tRNA_synth_B	Leu-tRNA-ligase	HAMAP
TIGR00396	leuS_bact	Leu-tRNA-ligase	NCBIFAM

Orthologs

LARS2 (HGNC:17095) Homo sapiens
SJAG_04738 Schizosaccharomyces japonicus
NAM2 (YLR382C) Saccharomyces cerevisiae

References / Literature

PMID:21511999 - Comparative functional genomics of the fission yeasts.: Rhind N et al. Science 2011 May 20;332(6032):930-6
GO_REF:0000002 - Comments
PMID:26670050 - Regulation of mRNA Levels by Decay-Promoting Introns that Recruit the Exosome Specificity Factor Mmi1.: Kilchert C et al. Cell Rep 2015 Dec 22;13(11):2504-2515
PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.: Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623
GO_REF:0000111 - Gene Ontology annotations Inferred by Curator (IC) using at least one Inferred by Sequence Similarity (ISS) annotation to support the inference
PMID:24763107 - Absolute proteome and phosphoproteome dynamics during the cell cycle of Schizosaccharomyces pombe (Fission Yeast).: Carpy A et al. Mol Cell Proteomics 2014 Aug;13(8):1925-36
GO_REF:0000033 - Annotation inferences using phylogenetic trees
PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.: Marguerat S et al. Cell 2012 Oct 26;151(3):671-83
PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.: Hayles J et al. Open Biol 2013 May 22;3(5):130053
PMID:16823372 - ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe.: Matsuyama A et al. Nat Biotechnol 2006 Jul;24(7):841-7
GO_REF:0000024 - Manual transfer of experimentally-verified manual GO annotation data to orthologs by curator judgment of sequence similarity.
PB_REF:0000006 - Disease associations from Monarch via human-pombe orthologs
PMID:36408920 - UniProt: the Universal Protein Knowledgebase in 2023.: UniProt Consortium Nucleic Acids Res 2023 Jan 06;51(D1):D523-D531
PMID:29294138 - Editor's Highlight: A Genome-wide Screening of Target Genes Against Silver Nanoparticles in Fission Yeast.: Lee AR et al. Toxicol Sci 2018 Jan 01;161(1):171-185