protein coding gene - srs2 (SPAC4H3.05) - ATP-dependent DNA helicase, UvrD subfamily

Gene summary

Standard name

srs2

Systematic ID

SPAC4H3.05

Product

ATP-dependent DNA helicase, UvrD subfamily

Organism

Schizosaccharomyces pombe (fission yeast)

UniProt ID

Q10213

ORFeome ID

47/47C06

Characterisation status

biological role published

Feature type

mRNA gene

Genomic location

chromosome I: 3832279..3835740 forward strand

Annotation

Protein features

Orthologs

• srs2 (SJAG_01221) Schizosaccharomyces japonicus
• SRS2 (YJL092W) Saccharomyces cerevisiae

References / Literature

PMID:16710300 - Sws1 is a conserved regulator of homologous recombination in eukaryotic cells.

Martín V et al. EMBO J 2006 Jun 07;25(11):2564-74

PMID:22366461 - Brc1-dependent recovery from replication stress.

Bass KL et al. J Cell Sci 2012 Jun 01;125(Pt 11):2753-64

PMID:16823372 - ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe.

Matsuyama A et al. Nat Biotechnol 2006 Jul;24(7):841-7

GO_REF:0000002 - Comments

PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.

Hayles J et al. Open Biol 2013 May 22;3(5):130053

PMID:18231579 - The CDK-activating kinase (CAK) Csk1 is required for normal levels of homologous recombination and resistance to DNA damage in fission yeast.

Gerber HB et al. PLoS One 2008 Jan 30;3(1):e1492

PMID:25165823 - Multiple regulation of Rad51-mediated homologous recombination by fission yeast Fbh1.

Tsutsui Y et al. PLoS Genet 2014 Aug;10(8):e1004542

PMID:22723423 - The fission yeast FANCM ortholog directs non-crossover recombination during meiosis.

Lorenz A et al. Science 2012 Jun 22;336(6088):1585-8

PMID:25313826 - The chromatin assembly factor 1 promotes Rad51-dependent template switches at replication forks by counteracting D-loop disassembly by the RecQ-type helicase Rqh1.

Pietrobon V et al. PLoS Biol 2014 Oct;12(10):e1001968

PMID:15485909 - Rad62 protein functionally and physically associates with the smc5/smc6 protein complex and is required for chromosome integrity and recombination repair in fission yeast.

Morikawa H et al. Mol Cell Biol 2004 Nov;24(21):9401-13

PMID:11452021 - Involvement of Schizosaccharomyces pombe Srs2 in cellular responses to DNA damage.

Wang SW et al. Nucleic Acids Res 2001 Jul 15;29(14):2963-72

PMID:24861625 - Hyperactive Cdc2 kinase interferes with the response to broken replication forks by trapping S.pombe Crb2 in its mitotic T215 phosphorylated state.

Mahyous Saeyd SA et al. Nucleic Acids Res 2014 Jul;42(12):7734-47

PMID:25720772 - Quantitative phosphoproteomics reveals pathways for coordination of cell growth and division by the conserved fission yeast kinase pom1.

Kettenbach AN et al. Mol Cell Proteomics 2015 May;14(5):1275-87

PMID:31149897 - The PCNA unloader Elg1 promotes recombination at collapsed replication forks in fission yeast.

Tamang S et al. Elife 2019 May 31;8

PMID:23828040 - Involvement of Schizosaccharomyces pombe rrp1+ and rrp2+ in the Srs2- and Swi5/Sfr1-dependent pathway in response to DNA damage and replication inhibition.

Dziadkowiec D et al. Nucleic Acids Res 2013 Sep;41(17):8196-209

PMID:18818364 - Conservation and rewiring of functional modules revealed by an epistasis map in fission yeast.

Roguev A et al. Science 2008 Oct 17;322(5900):405-10

PMID:33823663 - A TOR (target of rapamycin) and nutritional phosphoproteome of fission yeast reveals novel targets in networks conserved in humans.

Halova L et al. Open Biol 2021 Apr;11(4):200405

PMID:14993467 - The involvement of Srs2 in post-replication repair and homologous recombination in fission yeast.

Doe CL et al. Nucleic Acids Res 2004;32(4):1480-91

PMID:12409469 - The severe slow growth of Deltasrs2 Deltarqh1 in Schizosaccharomyces pombe is suppressed by loss of recombination and checkpoint genes.

Maftahi M et al. Nucleic Acids Res 2002 Nov 01;30(21):4781-92

PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.

Marguerat S et al. Cell 2012 Oct 26;151(3):671-83

PMID:29898918 - Mutations that prevent methylation of cohesin render sensitivity to DNA damage in S. pombe .

Sanyal S et al. J Cell Sci 2018 Jul 06;131(13)

PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.

Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623

PMID:16135799 - Role of the Schizosaccharomyces pombe F-Box DNA helicase in processing recombination intermediates.

Morishita T et al. Mol Cell Biol 2005 Sep;25(18):8074-83

PMID:19264558 - Screening a genome-wide S. pombe deletion library identifies novel genes and pathways involved in genome stability maintenance.

Deshpande GP et al. DNA Repair (Amst) 2009 May 01;8(5):672-9

PMID:16135800 - The F-Box DNA helicase Fbh1 prevents Rhp51-dependent recombination without mediator proteins.

Osman F et al. Mol Cell Biol 2005 Sep;25(18):8084-96

PMID:17515930 - Rad3-dependent phosphorylation of the checkpoint clamp regulates repair-pathway choice.

Kai M et al. Nat Cell Biol 2007 Jun;9(6):691-7

PMID:18769921 - Genetic analysis reveals different roles of Schizosaccharomyces pombe sfr1/dds20 in meiotic and mitotic DNA recombination and repair.

Khasanov FK et al. Curr Genet 2008 Oct;54(4):197-211

PMID:25795664 - Genetic Interaction Landscape Reveals Critical Requirements for Schizosaccharomyces pombe Brc1 in DNA Damage Response Mutants.

Sánchez A et al. G3 (Bethesda) 2015 Mar 19;5(5):953-62

PMID:24875629 - Essential domains of Schizosaccharomyces pombe Rad8 required for DNA damage response.

Ding L et al. G3 (Bethesda) 2014 May 28;4(8):1373-84

PMID:19037101 - Mus81, Rhp51(Rad51), and Rqh1 form an epistatic pathway required for the S-phase DNA damage checkpoint.

Willis N et al. Mol Biol Cell 2009 Feb;20(3):819-33

PMID:19416828 - Schizosaccharomyces pombe Rtf2 mediates site-specific replication termination by inhibiting replication restart.

Inagawa T et al. Proc Natl Acad Sci U S A 2009 May 12;106(19):7927-32

PMID:30667359 - Factors affecting template switch recombination associated with restarted DNA replication.

Jalan M et al. Elife 2019 Jan 22;8

PMID:16371652 - Production of reactive oxygen species in response to replication stress and inappropriate mitosis in fission yeast.

Marchetti MA et al. J Cell Sci 2006 Jan 01;119(Pt 1):124-31

PMID:37787768 - Broad functional profiling of fission yeast proteins using phenomics and machine learning.

Rodríguez-López M et al. Elife 2023 Oct 03;12

PMID:19547744 - Evolution of phosphoregulation: comparison of phosphorylation patterns across yeast species.

Beltrao P et al. PLoS Biol 2009 Jun 16;7(6):e1000134

PMID:30824696 - Systematic analysis reveals the prevalence and principles of bypassable gene essentiality.

Li J et al. Nat Commun 2019 Mar 01;10(1):1002

PMID:18426916 - The anaphase-promoting complex/cyclosome controls repair and recombination by ubiquitylating Rhp54 in fission yeast.

Trickey M et al. Mol Cell Biol 2008 Jun;28(12):3905-16

PMID:21511999 - Comparative functional genomics of the fission yeasts.

Rhind N et al. Science 2011 May 20;332(6032):930-6

PMID:19205745 - Redundant roles of Srs2 helicase and replication checkpoint in survival and rDNA maintenance in Schizosaccharomyces pombe.

Yasuhira S Mol Genet Genomics 2009 May;281(5):497-509

PMID:25373780 - A genomic Multiprocess survey of machineries that control and link cell shape, microtubule organization, and cell-cycle progression.

Graml V et al. Dev Cell 2014 Oct 27;31(2):227-239

PMID:36793083 - The SAGA histone acetyltransferase module targets SMC5/6 to specific genes.

Mahrik L et al. Epigenetics Chromatin 2023 Feb 16;16(1):6

PMID:33260998 - High-Throughput Flow Cytometry Combined with Genetic Analysis Brings New Insights into the Understanding of Chromatin Regulation of Cellular Quiescence.

Zahedi Y et al. Int J Mol Sci 2020 Nov 27;21(23)

PMID:18931302 - Significant conservation of synthetic lethal genetic interaction networks between distantly related eukaryotes.

Dixon SJ et al. Proc Natl Acad Sci U S A 2008 Oct 28;105(43):16653-8

PMID:21504829 - Yeast SREBP cleavage activation requires the Golgi Dsc E3 ligase complex.

Stewart EV et al. Mol Cell 2011 Apr 22;42(2):160-71

PMID:23173672 - Identification of novel genes involved in DNA damage response by screening a genome-wide Schizosaccharomyces pombe deletion library.

Pan X et al. BMC Genomics 2012 Nov 23;13:662

PMID:34250083 - Barcode sequencing and a high-throughput assay for chronological lifespan uncover ageing-associated genes in fission yeast.

Romila CA et al. Microb Cell 2021 Jul 05;8(7):146-160

GO_REF:0000033 - Annotation inferences using phylogenetic trees

PMID:21340088 - Microarray-based target identification using drug hypersensitive fission yeast expressing ORFeome.

Arita Y et al. Mol Biosyst 2011 May;7(5):1463-72

PMID:20176980 - Fission yeast Hsk1 (Cdc7) kinase is required after replication initiation for induced mutagenesis and proper response to DNA alkylation damage.

Dolan WP et al. Genetics 2010 May;185(1):39-53

PMID:22681890 - Hierarchical modularity and the evolution of genetic interactomes across species.

Ryan CJ et al. Mol Cell 2012 Jun 08;46(5):691-704

PMID:21712547 - Mitotic substrates of the kinase aurora with roles in chromatin regulation identified through quantitative phosphoproteomics of fission yeast.

Koch A et al. Sci Signal 2011 Jun 28;4(179):rs6

PMID:18722173 - Chromosome fusions following telomere loss are mediated by single-strand annealing.

Wang X et al. Mol Cell 2008 Aug 22;31(4):463-473

PMID:17893680 - High-throughput genetic interaction mapping in the fission yeast Schizosaccharomyces pombe.

Roguev A et al. Nat Methods 2007 Oct;4(10):861-6