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protein coding gene - pyk1 (SPAC4H3.10c) - pyruvate kinase

Gene summary

Standard name
pyk1
Systematic ID
SPAC4H3.10c
Product
pyruvate kinase
Organism
Schizosaccharomyces pombe (fission yeast)
UniProt ID
Q10208
ORFeome ID
24/24H01
Characterisation status
biological role published
Feature type
mRNA gene
Genomic location
chromosome I: 3844743..3846675 reverse strand

Annotation

Disease association

MONDO:0009950 - pyruvate kinase deficiency of red cells

References:

MONDO:0007067 - pyruvate kinase hyperactivity

References:

GO biological process

GO:0061621 - canonical glycolysis

References:

GO cellular component

GO:0005829 - cytosol

References:

GO molecular function

GO:0000287 - magnesium ion binding

References:

GO:0030955 - potassium ion binding

References:

GO:0004743 - pyruvate kinase activity

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Modification

MOD:00046 - O-phospho-L-serine

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MOD:00047 - O-phospho-L-threonine

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MOD:00048 - O4'-phospho-L-tyrosine

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MOD:00696 - phosphorylated residue

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MOD:01149 - sumoylated lysine

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MOD:01148 - ubiquitinylated lysine

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Multi-locus phenotype

FYPO:0007368 - increased energy charge

References:

Genotypes:

Qualitative gene expression

PomGeneEx:0000019 - protein level decreased

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PomGeneEx:0000027 - ribosomal density decreased

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PomGeneEx:0000011 - RNA level increased

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Quantitative gene expression

PBO:0006310 - protein level

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PBO:0011963 - RNA level

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Single locus phenotype

FYPO:0007369 - altered cellular redox status

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Genotypes:

FYPO:0007366 - decreased cellular 2,3-bisphosphoglyceric acid level

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Genotypes:

FYPO:0007365 - decreased cellular phosphoenolpyruvate level

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Genotypes:

FYPO:0002009 - decreased oxygen consumption during vegetative growth

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Genotypes:

FYPO:0007370 - increased cell population growth rate on fructose carbon source

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Genotypes:

FYPO:0000637 - increased cell population growth rate on glucose carbon source

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Genotypes:

FYPO:0007371 - increased cell population growth rate on sucrose carbon source

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Genotypes:

FYPO:0007367 - increased cellular fructose 1,6-bisphosphate level

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Genotypes:

FYPO:0007368 - increased energy charge

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Genotypes:

FYPO:0000433 - increased fermentation

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Genotypes:

FYPO:0007364 - increased pyruvate kinase activity

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Genotypes:

FYPO:0001309 - increased viability in stationary phase

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Genotypes:

FYPO:0002151 - inviable spore

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Genotypes:

FYPO:0002061 - inviable vegetative cell population

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Genotypes:

FYPO:0006518 - loss of viability in G0

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Genotypes:

FYPO:0000441 - resistance to antimycin A

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Genotypes:

FYPO:0000073 - resistance to caffeine

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Genotypes:

FYPO:0007372 - resistance to chlorpromazine

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Genotypes:

FYPO:0004398 - resistance to phenylarsine oxide

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Genotypes:

FYPO:0000799 - sensitive to diamide

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Genotypes:

FYPO:0004512 - sensitive to EGTA

References:

Genotypes:

FYPO:0000087 - sensitive to hydrogen peroxide

References:

Genotypes:

FYPO:0000726 - sensitive to oxidative stress

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Genotypes:

Subunit composition

PBO:0015212 - homomeric(2)

Taxonomic conservation

PBO:0011067 - conserved in bacteria

PBO:0011065 - conserved in eukaryotes

PBO:0011064 - conserved in fungi

PBO:0011069 - conserved in metazoa

PBO:0011070 - conserved in vertebrates

PBO:0006222 - predominantly single copy (one to one)

Protein features

IDNameInterPro nameDB name
PF00224PKPyrv_Knase_brlPFAM
PF02887PK_CPyrv_Knase_CPFAM
cd00288Pyruvate_KinasePyr_KnaseCDD
PS00110PYRUVATE_KINASEPyrv_Knase_ASPROSITE_PATTERNS
PR01050PYRUVTKNASEPyr_KnasePRINTS
G3DSA:2.40.33.10:FF:000001FUNFAM
G3DSA:3.20.20.60:FF:000025FUNFAM
SSF52935PK C-terminal domain-likePyrv_Knase_C_sfSUPERFAMILY
SSF50800PK beta-barrel domain-likePyrv_Knase-like_insert_dom_sfSUPERFAMILY
SSF51621Phosphoenolpyruvate/pyruvate domainPyrv/PenolPyrv_kinase-like_domSUPERFAMILY
G3DSA:2.40.33.10Pyrv_Knase_insert_dom_sfGENE3D
G3DSA:3.20.20.60Pyrv_kinase-like_dom_sfGENE3D
G3DSA:3.40.1380.20Pyrv_Knase_C_sfGENE3D
PTHR11817PYRUVATE KINASEPyr_KnasePANTHER
TIGR01064pyruv_kinPyr_KnaseNCBIFAM
NF004978PRK06354.1NCBIFAM
NF004491PRK05826.1NCBIFAM

Orthologs

References / Literature

PMID:26537787 - Targeting of SUMO substrates to a Cdc48-Ufd1-Npl4 segregase and STUbL pathway in fission yeast.
Køhler JB et al. Nat Commun 2015 Nov 05;6:8827
PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.
Hayles J et al. Open Biol 2013 May 22;3(5):130053
PMID:34496258 - Transcription and chromatin-based surveillance mechanism controls suppression of cryptic antisense transcription.
Heo DH et al. Cell Rep 2021 Sep 07;36(10):109671
PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.
Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623
PMID:37164017 - Optimization of energy production and central carbon metabolism in a non-respiring eukaryote.
Alam S et al. Curr Biol 2023 Jun 05;33(11):2175-2186.e5
PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.
Marguerat S et al. Cell 2012 Oct 26;151(3):671-83
PMID:26518661 - The Paf1 complex factors Leo1 and Paf1 promote local histone turnover to modulate chromatin states in fission yeast.
Sadeghi L et al. EMBO Rep 2015 Dec;16(12):1673-87
PMID:8586271 - Cloning and sequencing of a gene encoding pyruvate kinase from Schizosaccharomyces pombe; implications for quaternary structure and regulation of the enzyme.
Nairn J et al. FEMS Microbiol Lett 1995 Dec 15;134(2-3):221-6
PB_REF:0000006 - Disease associations from Monarch via human-pombe orthologs
PMID:16823372 - ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe.
Matsuyama A et al. Nat Biotechnol 2006 Jul;24(7):841-7
PMID:16537923 - Sterol regulatory element binding protein is a principal regulator of anaerobic gene expression in fission yeast.
Todd BL et al. Mol Cell Biol 2006 Apr;26(7):2817-31
PMID:18257517 - Phosphoproteome analysis of fission yeast.
Wilson-Grady JT et al. J Proteome Res 2008 Mar;7(3):1088-97
PMID:19366728 - Genetic control of cellular quiescence in S. pombe.
Sajiki K et al. J Cell Sci 2009 May 01;122(Pt 9):1418-29
PMID:19547744 - Evolution of phosphoregulation: comparison of phosphorylation patterns across yeast species.
Beltrao P et al. PLoS Biol 2009 Jun 16;7(6):e1000134
PMID:37939137 - The ortholog of human REEP1-4 is required for autophagosomal enclosure of ER-phagy/nucleophagy cargos in fission yeast.
Zou CX et al. PLoS Biol 2023 Nov;21(11):e3002372
PMID:31626996 - Multiplexed proteome profiling of carbon source perturbations in two yeast species with SL-SP3-TMT.
Paulo JA et al. J Proteomics 2020 Jan 06;210:103531
PMID:39367033 - Quantitative proteomics and phosphoproteomics profiling of meiotic divisions in the fission yeast Schizosaccharomyces pombe.
Sivakova B et al. Sci Rep 2024 Oct 04;14(1):23105
PMID:36361590 - Defining the Functional Interactome of Spliceosome-Associated G-Patch Protein Gpl1 in the Fission Yeast Schizosaccharomyces pombe .
Selicky T et al. Int J Mol Sci 2022 Oct 24;23(21)
PMID:27298342 - Identification of S-phase DNA damage-response targets in fission yeast reveals conservation of damage-response networks.
Willis NA et al. Proc Natl Acad Sci U S A 2016 Jun 28;113(26):E3676-85
GO_REF:0000002 - Comments
PMID:37970674 - SUMOylation regulates Lem2 function in centromere clustering and silencing.
Strachan J et al. J Cell Sci 2023 Dec 01;136(23)
PMID:21504829 - Yeast SREBP cleavage activation requires the Golgi Dsc E3 ligase complex.
Stewart EV et al. Mol Cell 2011 Apr 22;42(2):160-71
PMID:26404184 - High Confidence Fission Yeast SUMO Conjugates Identified by Tandem Denaturing Affinity Purification.
Nie M et al. Sci Rep 2015 Sep 25;5:14389
PMID:39358553 - Ageing-associated long non-coding RNA extends lifespan and reduces translation in non-dividing cells.
Anver S et al. EMBO Rep 2024 Oct 02;
PMID:9790887 - Purification and characterization of pyruvate kinase from Schizosaccharomyces pombe: evidence for an unusual quaternary structure.
Nairn J et al. Protein Expr Purif 1998 Nov;14(2):247-53
PMID:14008 - Study of the role of puring phosphoribosyltransferases in the uptake of adenine and guanine by Schizosaccharomyces pombe cells.
Housset P et al. Eur J Biochem 1977 Feb 15;73(1):99-105
PMID:24763107 - Absolute proteome and phosphoproteome dynamics during the cell cycle of Schizosaccharomyces pombe (Fission Yeast).
Carpy A et al. Mol Cell Proteomics 2014 Aug;13(8):1925-36
PMID:29996109 - Quantitative Phosphoproteomics Reveals the Signaling Dynamics of Cell-Cycle Kinases in the Fission Yeast Schizosaccharomyces pombe.
Swaffer MP et al. Cell Rep 2018 Jul 10;24(2):503-514
PMID:33313903 - Ribosome profiling reveals ribosome stalling on tryptophan codons and ribosome queuing upon oxidative stress in fission yeast.
Rubio A et al. Nucleic Acids Res 2021 Jan 11;49(1):383-399
PMID:40015273 - A comprehensive Schizosaccharomyces pombe atlas of physical transcription factor interactions with proteins and chromatin.
Skribbe M et al. Mol Cell 2025 Feb 19;
PMID:32319721 - Pyruvate kinase variant of fission yeast tunes carbon metabolism, cell regulation, growth and stress resistance.
Kamrad S et al. Mol Syst Biol 2020 Apr;16(4):e9270
PMID:33823663 - A TOR (target of rapamycin) and nutritional phosphoproteome of fission yeast reveals novel targets in networks conserved in humans.
Halova L et al. Open Biol 2021 Apr;11(4):200405
PMID:28218250 - Chromatin remodeller Fun30 Fft3 induces nucleosome disassembly to facilitate RNA polymerase II elongation.
Lee J et al. Nat Commun 2017 Feb 20;8:14527
PMID:21511999 - Comparative functional genomics of the fission yeasts.
Rhind N et al. Science 2011 May 20;332(6032):930-6
PMID:39705284 - Proteomic and phosphoproteomic analyses reveal that TORC1 is reactivated by pheromone signaling during sexual reproduction in fission yeast.
Bérard M et al. PLoS Biol 2024 Dec 20;22(12):e3002963
PMID:24634168 - Proteome-wide search for PP2A substrates in fission yeast.
Bernal M et al. Proteomics 2014 Jun;14(11):1367-80
PMID:25720772 - Quantitative phosphoproteomics reveals pathways for coordination of cell growth and division by the conserved fission yeast kinase pom1.
Kettenbach AN et al. Mol Cell Proteomics 2015 May;14(5):1275-87
PMID:30726745 - Fission Yeast NDR/LATS Kinase Orb6 Regulates Exocytosis via Phosphorylation of the Exocyst Complex.
Tay YD et al. Cell Rep 2019 Feb 05;26(6):1654-1667.e7
PMID:21652630 - Characterization of Mug33 reveals complementary roles for actin cable-dependent transport and exocyst regulators in fission yeast exocytosis.
Snaith HA et al. J Cell Sci 2011 Jul 01;124(Pt 13):2187-99
PMID:26412298 - A Degenerate Cohort of Yeast Membrane Trafficking DUBs Mediates Cell Polarity and Survival.
Beckley JR et al. Mol Cell Proteomics 2015 Dec;14(12):3132-41