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protein coding gene - met9 (SPAC56F8.10) - methylenetetrahydrofolate reductase Met9

Gene summary

Standard name
met9
Systematic ID
SPAC56F8.10
Product
methylenetetrahydrofolate reductase Met9
Organism
Schizosaccharomyces pombe (fission yeast)
Synonyms
met5
UniProt ID
Q10258
ORFeome ID
36/36E12
Characterisation status
biological role published
Feature type
mRNA gene
Genomic location
chromosome I: 1143065..1145256 forward strand

Annotation

Disease association

MONDO:0000819 - anencephaly

References:

MONDO:0009353 - homocystinuria due to methylene tetrahydrofolate reductase deficiency

References:

MONDO:0005071 - nervous system disorder

References:

MONDO:0011120 - neural tube defects, folate-sensitive

References:

MONDO:0005090 - schizophrenia

References:

MONDO:0008449 - spina bifida

References:

MONDO:0008559 - thrombophilia due to thrombin defect

References:

GO biological process

GO:0071265 - L-methionine biosynthetic process

References:

GO:0035999 - tetrahydrofolate interconversion

References:

GO cellular component

GO:0005829 - cytosol

References:

GO:0005739 - mitochondrion

References:

GO molecular function

GO:0071949 - FAD binding

References:

GO:0106312 - methylenetetrahydrofolate reductase (NADH) activity

References:

GO:0106313 - methylenetetrahydrofolate reductase (NADPH) activity

References:

GO:0004489 - methylenetetrahydrofolate reductase [NAD(P)H] activity

References:

Modification

MOD:00046 - O-phospho-L-serine

References:

MOD:00047 - O-phospho-L-threonine

References:

MOD:00696 - phosphorylated residue

References:

Qualitative gene expression

PomGeneEx:0000019 - protein level decreased

References:

PomGeneEx:0000012 - RNA level decreased

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Quantitative gene expression

PBO:0006310 - protein level

References:

PBO:0011963 - RNA level

References:

Single locus phenotype

FYPO:0000400 - abnormal cell cycle arrest at mitotic G2/M phase transition

References:

Genotypes:

FYPO:0002697 - decreased methylenetetrahydrofolate reductase (NADPH) activity

References:

Genotypes:

FYPO:0000040 - growth auxotrophic for methionine

References:

Genotypes:

FYPO:0003004 - increased cellular reactive oxygen species level during vegetative growth

References:

Genotypes:

FYPO:0002698 - increased methylenetetrahydrofolate reductase (NADPH) activity

References:

Genotypes:

FYPO:0000311 - inviable after spore germination with normal, unseptated germ tube morphology

References:

Genotypes:

FYPO:0002061 - inviable vegetative cell population

References:

Genotypes:

FYPO:0003902 - resistance to etoposide

References:

Genotypes:

FYPO:0000750 - sensitive to silver ions

References:

Genotypes:

FYPO:0006929 - sensitive to silver nanoparticles

References:

Genotypes:

FYPO:0001492 - viable elongated vegetative cell

References:

Genotypes:

Taxonomic conservation

PBO:0011067 - conserved in bacteria

PBO:0011065 - conserved in eukaryotes

PBO:0011064 - conserved in fungi

PBO:0011069 - conserved in metazoa

PBO:0011070 - conserved in vertebrates

PBO:0006222 - predominantly single copy (one to one)

Protein features

IDNameInterPro nameDB name
PF02219MTHFRMehydrof_redctse-likePfam
PF21895MTHFR_CMTHFR_CPfam
cd00537MTHFRMehydrof_redctse-likeCDD
G3DSA:3.20.20.220:FF:000002CATH-FunFam
G3DSA:3.20.20.220CATH-Gene3D
SSF51730FAD-linked_oxidoreductase-likeSUPERFAMILY
PTHR45754PANTHER
TIGR00677fadh2_eukFadh2_eukNCBIFAM

Orthologs

References / Literature

PB_REF:0000006 - Disease associations from Monarch via human-pombe orthologs
PMID:27984744 - Survival in Quiescence Requires the Euchromatic Deployment of Clr4/SUV39H by Argonaute-Associated Small RNAs.
Joh RI et al. Mol Cell 2016 Dec 15;64(6):1088-1101
PMID:39705284 - Proteomic and phosphoproteomic analyses reveal that TORC1 is reactivated by pheromone signaling during sexual reproduction in fission yeast.
Bérard M et al. PLoS Biol 2024 Dec 20;22(12):e3002963
PMID:12112238 - Two non-complementing genes encoding enzymatically active methylenetetrahydrofolate reductases control methionine requirement in fission yeast Schizosaccharomyces pombe.
Naula N et al. Yeast 2002 Jul;19(10):841-8
GO_REF:0000116 - Automatic Gene Ontology annotation based on Rhea mapping.
PMID:25720772 - Quantitative phosphoproteomics reveals pathways for coordination of cell growth and division by the conserved fission yeast kinase pom1.
Kettenbach AN et al. Mol Cell Proteomics 2015 May;14(5):1275-87
PMID:39367033 - Quantitative proteomics and phosphoproteomics profiling of meiotic divisions in the fission yeast Schizosaccharomyces pombe.
Sivakova B et al. Sci Rep 2024 Oct 04;14(1):23105
PMID:17248775 - Genetic Mapping in SCHIZOSACCHAROMYCES POMBE by Mitotic and Meiotic Analysis and Induced Haploidization.
Kohli J et al. Genetics 1977 Nov;87(3):471-89
PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.
Marguerat S et al. Cell 2012 Oct 26;151(3):671-83
PMID:19547744 - Evolution of phosphoregulation: comparison of phosphorylation patterns across yeast species.
Beltrao P et al. PLoS Biol 2009 Jun 16;7(6):e1000134
PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.
Hayles J et al. Open Biol 2013 May 22;3(5):130053
PMID:21511999 - Comparative functional genomics of the fission yeasts.
Rhind N et al. Science 2011 May 20;332(6032):930-6
PMID:29996109 - Quantitative Phosphoproteomics Reveals the Signaling Dynamics of Cell-Cycle Kinases in the Fission Yeast Schizosaccharomyces pombe.
Swaffer MP et al. Cell Rep 2018 Jul 10;24(2):503-514
GO_REF:0000033 - Annotation inferences using phylogenetic trees
PMID:18257517 - Phosphoproteome analysis of fission yeast.
Wilson-Grady JT et al. J Proteome Res 2008 Mar;7(3):1088-97
PMID:22754237 - Methylenetetrahydrofolate reductase mutations, a genetic cause for familial recurrent neural tube defects.
Yaliwal LV et al. Indian J Hum Genet 2012 Jan;18(1):122-4
PMID:26771498 - A Proteome-wide Fission Yeast Interactome Reveals Network Evolution Principles from Yeasts to Human.
Vo TV et al. Cell 2016 Jan 14;164(1-2):310-323
PMID:28469148 - Functional organization of protein determinants of meiotic DNA break hotspots.
Ma L et al. Sci Rep 2017 May 03;7(1):1393
PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.
Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623
PMID:20519959 - S. pombe genome deletion project: an update.
Spirek M et al. Cell Cycle 2010 Jun 15;9(12):2399-402
PMID:21340088 - Microarray-based target identification using drug hypersensitive fission yeast expressing ORFeome.
Arita Y et al. Mol Biosyst 2011 May;7(5):1463-72
PMID:29294138 - Editor's Highlight: A Genome-wide Screening of Target Genes Against Silver Nanoparticles in Fission Yeast.
Lee AR et al. Toxicol Sci 2018 Jan 01;161(1):171-185
PMID:24763107 - Absolute proteome and phosphoproteome dynamics during the cell cycle of Schizosaccharomyces pombe (Fission Yeast).
Carpy A et al. Mol Cell Proteomics 2014 Aug;13(8):1925-36
PMID:30726745 - Fission Yeast NDR/LATS Kinase Orb6 Regulates Exocytosis via Phosphorylation of the Exocyst Complex.
Tay YD et al. Cell Rep 2019 Feb 05;26(6):1654-1667.e7
PMID:33823663 - A TOR (target of rapamycin) and nutritional phosphoproteome of fission yeast reveals novel targets in networks conserved in humans.
Halova L et al. Open Biol 2021 Apr;11(4):200405
GO_REF:0000024 - Manual transfer of experimentally-verified manual GO annotation data to orthologs by curator judgment of sequence similarity.