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protein coding gene - ura3 (SPAC57A10.12c) - mitochondrial dihydroorotate dehydrogenase Ura3

Gene summary

Standard name
ura3
Systematic ID
SPAC57A10.12c
Product
mitochondrial dihydroorotate dehydrogenase Ura3
Organism
Schizosaccharomyces pombe (fission yeast)
UniProt ID
P32747
ORFeome ID
20/20F02
Characterisation status
biological role published
Feature type
mRNA gene
Genomic location
chromosome I: 1385357..1387145 reverse strand

Annotation

Complementation

PBO:0013022 - functionally complemented by human DHODH

References:

PBO:0011087 - functionally complements S. cerevisiae URA1

References:

Disease association

MONDO:0009903 - postaxial acrofacial dysostosis

References:

GO biological process

GO:0006207 - 'de novo' pyrimidine nucleobase biosynthetic process

References:

GO:0044205 - 'de novo' UMP biosynthetic process

References:

GO cellular component

GO:0005743 - mitochondrial inner membrane

References:

GO:0005739 - mitochondrion

References:

GO molecular function

GO:0106430 - dihydroorotate dehydrogenase (quinone) activity

References:

GO:0004152 - dihydroorotate dehydrogenase activity

References:

Modification

MOD:00046 - O-phospho-L-serine

References:

MOD:01148 - ubiquitinylated lysine

References:

Protein sequence feature

SO:0001808 - mitochondrial_targeting_signal

References:

Qualitative gene expression

PomGeneEx:0000012 - RNA level decreased

References:

Quantitative gene expression

PBO:0006310 - protein level

References:

PBO:0011963 - RNA level

References:

Single locus phenotype

FYPO:0000121 - abnormal sporulation

References:

Genotypes:

FYPO:0004587 - abolished cell population growth on urea nitrogen source

References:

Genotypes:

FYPO:0003412 - decreased chromatin silencing at centromere outer repeat

References:

Genotypes:

FYPO:0001012 - growth auxotrophic for uracil

References:

Genotypes:

FYPO:0003902 - resistance to etoposide

References:

Genotypes:

FYPO:0002865 - sensitive to polypeptone

References:

Genotypes:

FYPO:0002060 - viable vegetative cell population

References:

Genotypes:

FYPO:0002197 - viable vegetative cell with abnormal cell shape

References:

Genotypes:

FYPO:0002177 - viable vegetative cell with normal cell morphology

References:

Genotypes:

Taxonomic conservation

PBO:0011067 - conserved in bacteria

PBO:0011065 - conserved in eukaryotes

PBO:0011064 - conserved in fungi

PBO:0011069 - conserved in metazoa

PBO:0011070 - conserved in vertebrates

PBO:0006222 - predominantly single copy (one to one)

Protein features

IDNameInterPro nameDB name
PF01180DHO_dhDihydroorotate_DH_catPfam
cd04738DHOD_2_likeDihydroorotate_DH_2CDD
PS00911DHODEHASE_1Dihydroorotate_DH_CSPROSITE patterns
PS00912DHODEHASE_2Dihydroorotate_DH_CSPROSITE patterns
G3DSA:3.20.20.70:FF:000066CATH-FunFam
G3DSA:3.20.20.70Aldolase_TIMCATH-Gene3D
SSF51395SUPERFAMILY
PTHR48109DHO_dehydrogenasePANTHER
TIGR01036pyrD_sub2Dihydroorotate_DH_2NCBIFAM

Orthologs

References / Literature

PMID:28218250 - Chromatin remodeller Fun30 Fft3 induces nucleosome disassembly to facilitate RNA polymerase II elongation.
Lee J et al. Nat Commun 2017 Feb 20;8:14527
PMID:1409592 - Divergent evolution of pyrimidine biosynthesis between anaerobic and aerobic yeasts.
Nagy M et al. Proc Natl Acad Sci U S A 1992 Oct 01;89(19):8966-70
GO_REF:0000033 - Annotation inferences using phylogenetic trees
PMID:18257517 - Phosphoproteome analysis of fission yeast.
Wilson-Grady JT et al. J Proteome Res 2008 Mar;7(3):1088-97
PMID:28345447 - Urea enhances cell lysis of Schizosaccharomyces pombe ura4 mutants.
Nishino K et al. Biosci Biotechnol Biochem 2017 Jul;81(7):1444-1451
PMID:15196933 - Two different dihydroorotate dehydrogenases from yeast Saccharomyces kluyveri.
Zameitat E et al. FEBS Lett 2004 Jun 18;568(1-3):129-34
PMID:17248775 - Genetic Mapping in SCHIZOSACCHAROMYCES POMBE by Mitotic and Meiotic Analysis and Induced Haploidization.
Kohli J et al. Genetics 1977 Nov;87(3):471-89
GO_REF:0000116 - Automatic Gene Ontology annotation based on Rhea mapping.
PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.
Marguerat S et al. Cell 2012 Oct 26;151(3):671-83
PMID:29259000 - Genes Important for Schizosaccharomyces pombe Meiosis Identified Through a Functional Genomics Screen.
Blyth J et al. Genetics 2018 Feb;208(2):589-603
PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.
Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623
PMID:37970674 - SUMOylation regulates Lem2 function in centromere clustering and silencing.
Strachan J et al. J Cell Sci 2023 Dec 01;136(23)
PMID:21340088 - Microarray-based target identification using drug hypersensitive fission yeast expressing ORFeome.
Arita Y et al. Mol Biosyst 2011 May;7(5):1463-72
PMID:24763107 - Absolute proteome and phosphoproteome dynamics during the cell cycle of Schizosaccharomyces pombe (Fission Yeast).
Carpy A et al. Mol Cell Proteomics 2014 Aug;13(8):1925-36
PMID:22692683 - Miller (Genee-Wiedemann) syndrome represents a clinically and biochemically distinct subgroup of postaxial acrofacial dysostosis associated with partial deficiency of DHODH.
Rainger J et al. Hum Mol Genet 2012 Sep 15;21(18):3969-83
PMID:25373780 - A genomic Multiprocess survey of machineries that control and link cell shape, microtubule organization, and cell-cycle progression.
Graml V et al. Dev Cell 2014 Oct 27;31(2):227-239
PMID:40015273 - A comprehensive Schizosaccharomyces pombe atlas of physical transcription factor interactions with proteins and chromatin.
Skribbe M et al. Mol Cell 2025 Feb 19;
PMID:36408920 - UniProt: the Universal Protein Knowledgebase in 2023.
UniProt Consortium Nucleic Acids Res 2023 Jan 06;51(D1):D523-D531
PMID:21712547 - Mitotic substrates of the kinase aurora with roles in chromatin regulation identified through quantitative phosphoproteomics of fission yeast.
Koch A et al. Sci Signal 2011 Jun 28;4(179):rs6
PMID:21511999 - Comparative functional genomics of the fission yeasts.
Rhind N et al. Science 2011 May 20;332(6032):930-6
PMID:21504829 - Yeast SREBP cleavage activation requires the Golgi Dsc E3 ligase complex.
Stewart EV et al. Mol Cell 2011 Apr 22;42(2):160-71
PMID:22681890 - Hierarchical modularity and the evolution of genetic interactomes across species.
Ryan CJ et al. Mol Cell 2012 Jun 08;46(5):691-704
PMID:26412298 - A Degenerate Cohort of Yeast Membrane Trafficking DUBs Mediates Cell Polarity and Survival.
Beckley JR et al. Mol Cell Proteomics 2015 Dec;14(12):3132-41
PMID:33313903 - Ribosome profiling reveals ribosome stalling on tryptophan codons and ribosome queuing upon oxidative stress in fission yeast.
Rubio A et al. Nucleic Acids Res 2021 Jan 11;49(1):383-399
PMID:32101745 - Dense Transposon Integration Reveals Essential Cleavage and Polyadenylation Factors Promote Heterochromatin Formation.
Lee SY et al. Cell Rep 2020 Feb 25;30(8):2686-2698.e8
PMID:12529438 - Global transcriptional responses of fission yeast to environmental stress.
Chen D et al. Mol Biol Cell 2003 Jan;14(1):214-29
PMID:28357272 - A central role for TOR signalling in a yeast model for juvenile CLN3 disease.
Bond ME et al. Microb Cell 2015 Nov 11;2(12):466-480
PMID:27984744 - Survival in Quiescence Requires the Euchromatic Deployment of Clr4/SUV39H by Argonaute-Associated Small RNAs.
Joh RI et al. Mol Cell 2016 Dec 15;64(6):1088-1101
PB_REF:0000006 - Disease associations from Monarch via human-pombe orthologs
PMID:23555823 - Polypeptone induces dramatic cell lysis in ura4 deletion mutants of fission yeast.
Matsuo Y et al. PLoS One 2013;8(3):e59887
PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.
Hayles J et al. Open Biol 2013 May 22;3(5):130053