PomBase home

protein coding gene - mip1 (SPAC57A7.11) - TORC1 substrate adaptor, WD repeat protein, Raptor homolog Mip1

Gene summary

Standard name
mip1
Systematic ID
SPAC57A7.11
Product
TORC1 substrate adaptor, WD repeat protein, Raptor homolog Mip1
Organism
Schizosaccharomyces pombe (fission yeast)
UniProt ID
P87141
ORFeome ID
39/39D06
Characterisation status
biological role published
Feature type
mRNA gene
Genomic location
chromosome I: 1517223..1521442 reverse strand

Annotation

GO biological process

GO:0009267 - cellular response to starvation

References:

GO:0031139 - positive regulation of conjugation with cellular fusion

References:

GO:0010506 - regulation of autophagy

References:

GO:0038202 - TORC1 signaling

References:

GO cellular component

GO:0005737 - cytoplasm

References:

GO:0000329 - fungal-type vacuole membrane

References:

GO:0030874 - nucleolar chromatin

References:

GO:0031931 - TORC1 complex

References:

GO molecular function

GO:0005515 - protein binding

References:

GO:0030674 - protein-macromolecule adaptor activity

References:

Modification

MOD:00046 - O-phospho-L-serine

References:

MOD:00047 - O-phospho-L-threonine

References:

MOD:00696 - phosphorylated residue

References:

Multi-locus phenotype

FYPO:0002060 - viable vegetative cell population

References:

Genotypes:

Protein features

PBO:0111751 - WD repeat protein

Qualitative gene expression

PomGeneEx:0000018 - protein level increased

References:

PomGeneEx:0000011 - RNA level increased

References:

Quantitative gene expression

PBO:0006310 - protein level

References:

PBO:0011963 - RNA level

References:

Single locus phenotype

FYPO:0001430 - abnormal mitotic cell cycle arrest with unreplicated DNA

References:

Genotypes:

FYPO:0002224 - abnormally arrested meiotic cell cycle

References:

Genotypes:

FYPO:0000303 - decreased conjugation frequency

References:

Genotypes:

FYPO:0001838 - decreased protein phosphorylation during vegetative growth

References:

Genotypes:

FYPO:0001645 - decreased protein-protein interaction

References:

Genotypes:

FYPO:0001152 - decreased RNA level during nitrogen starvation

References:

Genotypes:

FYPO:0000581 - decreased spore germination frequency

References:

Genotypes:

FYPO:0000584 - decreased sporulation frequency

References:

Genotypes:

FYPO:0000017 - elongated cell

References:

Genotypes:

FYPO:0005258 - increased cell population growth at high temperature

References:

Genotypes:

FYPO:0001327 - increased protein level during vegetative growth

References:

Genotypes:

FYPO:0001130 - increased protein localization to nucleus during vegetative growth

References:

Genotypes:

FYPO:0002430 - inviable after spore germination, multiple cell divisions

References:

Genotypes:

FYPO:0001042 - inviable after spore germination, single or double cell division

References:

Genotypes:

FYPO:0000313 - inviable after spore germination, without cell division, with abnormal germ tube morphology

References:

Genotypes:

FYPO:0000951 - inviable small vegetative cell

References:

Genotypes:

FYPO:0002061 - inviable vegetative cell population

References:

Genotypes:

FYPO:0001147 - normal mating efficiency

References:

Genotypes:

FYPO:0004248 - normal protein localization to vacuolar membrane

References:

Genotypes:

FYPO:0000776 - normal protein phosphorylation during vegetative growth

References:

Genotypes:

FYPO:0000703 - normal protein-protein interaction

References:

Genotypes:

FYPO:0001357 - normal vegetative cell population growth

References:

Genotypes:

FYPO:0000111 - sensitive to rapamycin

References:

Genotypes:

FYPO:0001234 - slow vegetative cell population growth

References:

Genotypes:

FYPO:0002060 - viable vegetative cell population

References:

Genotypes:

Taxonomic conservation

PBO:0011065 - conserved in eukaryotes

PBO:0011071 - conserved in eukaryotes only

PBO:0011064 - conserved in fungi

PBO:0011069 - conserved in metazoa

PBO:0011070 - conserved in vertebrates

PBO:0006222 - predominantly single copy (one to one)

Protein features

IDNameInterPro nameDB name
PF00400WD40WD40_rptPFAM
PF14538Raptor_NRaptor_NPFAM
PS00678WD_REPEATS_1WD40_repeat_CSPROSITE_PATTERNS
PS50082WD_REPEATS_2WD40_rptPROSITE_PROFILES
PS50294WD_REPEATS_REGIONPROSITE_PROFILES
SM01302Raptor_N_2Raptor_NSMART
SM00320WD40_4WD40_rptSMART
PR01547YEAST176DUFPRINTS
SSF48371ARM repeatARM-type_foldSUPERFAMILY
SSF50978WD40 repeat-likeWD40_repeat_dom_sfSUPERFAMILY
G3DSA:2.130.10.10WD40/YVTN_repeat-like_dom_sfGENE3D
G3DSA:1.25.10.10ARM-likeGENE3D
PTHR12848REGULATORY-ASSOCIATED PROTEIN OF MTORRaptorPANTHER
mobidb-lite-Disorderdisorder_predictionMOBIDB-Disorder

Orthologs

References / Literature

PMID:40015273 - A comprehensive Schizosaccharomyces pombe atlas of physical transcription factor interactions with proteins and chromatin.
Skribbe M et al. Mol Cell 2025 Feb 19;
PMID:16823372 - ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe.
Matsuyama A et al. Nat Biotechnol 2006 Jul;24(7):841-7
PMID:17046992 - Fission yeast Tor2 promotes cell growth and represses cell differentiation.
Alvarez B et al. J Cell Sci 2006 Nov 01;119(Pt 21):4475-85
PMID:35286199 - Mitotic spindle formation in the absence of Polo kinase.
Kim J et al. Proc Natl Acad Sci U S A 2022 Mar 22;119(12):e2114429119
PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.
Hayles J et al. Open Biol 2013 May 22;3(5):130053
PMID:19547744 - Evolution of phosphoregulation: comparison of phosphorylation patterns across yeast species.
Beltrao P et al. PLoS Biol 2009 Jun 16;7(6):e1000134
PMID:10648609 - Novel WD-repeat protein Mip1p facilitates function of the meiotic regulator Mei2p in fission yeast.
Shinozaki-Yabana S et al. Mol Cell Biol 2000 Feb;20(4):1234-42
PMID:22762302 - TORC1 of fission yeast is rapamycin-sensitive.
Takahara T et al. Genes Cells 2012 Aug;17(8):698-708
PMID:27298342 - Identification of S-phase DNA damage-response targets in fission yeast reveals conservation of damage-response networks.
Willis NA et al. Proc Natl Acad Sci U S A 2016 Jun 28;113(26):E3676-85
PMID:33823663 - A TOR (target of rapamycin) and nutritional phosphoproteome of fission yeast reveals novel targets in networks conserved in humans.
Halova L et al. Open Biol 2021 Apr;11(4):200405
PMID:37913773 - TOR inactivation triggers heterochromatin formation in rDNA during glucose starvation.
Hirai H et al. Cell Rep 2023 Nov 28;42(11):113320
PMID:29199950 - Ragulator and GATOR1 complexes promote fission yeast growth by attenuating TOR complex 1 through Rag GTPases.
Chia KH et al. Elife 2017 Dec 04;6
PMID:39476757 - Characterization of Ksg1 protein kinase-dependent phosphoproteome in the fission yeast S. pombe.
Cipak L et al. Biochem Biophys Res Commun 2024 Oct 25;736:150895
GO_REF:0000033 - Annotation inferences using phylogenetic trees
PMID:12529438 - Global transcriptional responses of fission yeast to environmental stress.
Chen D et al. Mol Biol Cell 2003 Jan;14(1):214-29
PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.
Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623
PMID:36481249 - Critical role of Wat1/Pop3 in regulating the TORC1 signalling pathway in fission yeast S. pombe.
Panigrahi L et al. Fungal Genet Biol 2023 Jan;164:103764
PMID:18257517 - Phosphoproteome analysis of fission yeast.
Wilson-Grady JT et al. J Proteome Res 2008 Mar;7(3):1088-97
PMID:28264193 - Substrate specificity of TOR complex 2 is determined by a ubiquitin-fold domain of the Sin1 subunit.
Tatebe H et al. Elife 2017 Mar 07;6
PMID:29996109 - Quantitative Phosphoproteomics Reveals the Signaling Dynamics of Cell-Cycle Kinases in the Fission Yeast Schizosaccharomyces pombe.
Swaffer MP et al. Cell Rep 2018 Jul 10;24(2):503-514
PMID:24763107 - Absolute proteome and phosphoproteome dynamics during the cell cycle of Schizosaccharomyces pombe (Fission Yeast).
Carpy A et al. Mol Cell Proteomics 2014 Aug;13(8):1925-36
PMID:18076573 - Rapamycin sensitivity of the Schizosaccharomyces pombe tor2 mutant and organization of two highly phosphorylated TOR complexes by specific and common subunits.
Hayashi T et al. Genes Cells 2007 Dec;12(12):1357-70
PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.
Marguerat S et al. Cell 2012 Oct 26;151(3):671-83
PMID:17261596 - Loss of the TOR kinase Tor2 mimics nitrogen starvation and activates the sexual development pathway in fission yeast.
Matsuo T et al. Mol Cell Biol 2007 Apr;27(8):3154-64
PMID:39367033 - Quantitative proteomics and phosphoproteomics profiling of meiotic divisions in the fission yeast Schizosaccharomyces pombe.
Sivakova B et al. Sci Rep 2024 Oct 04;14(1):23105
PMID:19417002 - Fission yeast Tor1 functions as part of TORC1 to control mitotic entry through the stress MAPK pathway following nutrient stress.
Hartmuth S et al. J Cell Sci 2009 Jun 01;122(Pt 11):1737-46
PMID:22344254 - The Vam6 and Gtr1-Gtr2 pathway activates TORC1 in response to amino acids in fission yeast.
Valbuena N et al. J Cell Sci 2012 Apr 15;125(Pt 8):1920-8
PMID:24741065 - S. pombe TORC1 activates the ubiquitin-proteasomal degradation of the meiotic regulator Mei2 in cooperation with Pat1 kinase.
Otsubo Y et al. J Cell Sci 2014 Jun 15;127(Pt 12):2639-46
PMID:26152587 - TORC1 Regulates Developmental Responses to Nitrogen Stress via Regulation of the GATA Transcription Factor Gaf1.
Laor D et al. mBio 2015 Jul 07;6(4):e00959
PMID:21511999 - Comparative functional genomics of the fission yeasts.
Rhind N et al. Science 2011 May 20;332(6032):930-6
PMID:22645648 - The reverse, but coordinated, roles of Tor2 (TORC1) and Tor1 (TORC2) kinases for growth, cell cycle and separase-mediated mitosis in Schizosaccharomyces pombe.
Ikai N et al. Open Biol 2011 Nov;1(3):110007
PMID:34499159 - Fission yeast TOR complex 1 phosphorylates Psk1 through an evolutionarily conserved interaction mediated by the TOS motif.
Morozumi Y et al. J Cell Sci 2021 Oct 01;134(19)
PMID:25720772 - Quantitative phosphoproteomics reveals pathways for coordination of cell growth and division by the conserved fission yeast kinase pom1.
Kettenbach AN et al. Mol Cell Proteomics 2015 May;14(5):1275-87
PMID:21712547 - Mitotic substrates of the kinase aurora with roles in chromatin regulation identified through quantitative phosphoproteomics of fission yeast.
Koch A et al. Sci Signal 2011 Jun 28;4(179):rs6
PMID:24766403 - Mudi, a web tool for identifying mutations by bioinformatics analysis of whole-genome sequence.
Iida N et al. Genes Cells 2014 Jun;19(6):517-27
PMID:38269097 - Rapamycin-sensitive mechanisms confine the growth of fission yeast below the temperatures detrimental to cell physiology.
Morozumi Y et al. iScience 2024 Jan 19;27(1):108777
PMID:30726745 - Fission Yeast NDR/LATS Kinase Orb6 Regulates Exocytosis via Phosphorylation of the Exocyst Complex.
Tay YD et al. Cell Rep 2019 Feb 05;26(6):1654-1667.e7
PMID:39910760 - Novel TORC1 inhibitor Ecl1 is regulated by phosphorylation in fission yeast.
Ohtsuka H et al. Aging Cell 2025 Feb 05;:e14450