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protein coding gene - dam1 (SPAC589.08c) - DASH complex subunit Dam1

Gene summary

Standard name
dam1
Systematic ID
SPAC589.08c
Product
DASH complex subunit Dam1
Organism
Schizosaccharomyces pombe (fission yeast)
UniProt ID
Q9HDZ6
ORFeome ID
41/41C05
Characterisation status
biological role published
Feature type
mRNA gene
Genomic location
chromosome I: 3106191..3106875 reverse strand

Annotation

GO biological process

GO:0051315 - attachment of mitotic spindle microtubules to kinetochore

References:

GO:0031619 - homologous chromosome orientation in meiotic metaphase I

References:

GO:0007019 - microtubule depolymerization

References:

GO:1990942 - mitotic metaphase chromosome recapture

References:

GO:1990758 - mitotic sister chromatid biorientation

References:

GO:0061804 - mitotic spindle formation (spindle phase one)

References:

GO:1990976 - protein transport along microtubule to mitotic spindle pole body

References:

GO:0140274 - repair of kinetochore microtubule attachment defect

References:

GO:0051455 - spindle attachment to meiosis I kinetochore

References:

GO cellular component

GO:0000779 - condensed chromosome, centromeric region

References:

GO:0055028 - cortical microtubule

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GO:0042729 - DASH complex

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GO:0000776 - kinetochore

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GO:0072686 - mitotic spindle

References:

GO:1990537 - mitotic spindle polar microtubule

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GO:0044732 - mitotic spindle pole body

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GO:0005634 - nucleus

References:

GO:0000940 - outer kinetochore

References:

Modification

MOD:00046 - O-phospho-L-serine

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MOD:00047 - O-phospho-L-threonine

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MOD:00696 - phosphorylated residue

References:

MOD:01149 - sumoylated lysine

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Multi-locus phenotype

FYPO:0000620 - abnormal cell cycle arrest in mitotic metaphase

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Genotypes:

FYPO:0001779 - abnormal centromere clustering at nuclear periphery during vegetative growth

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Genotypes:

FYPO:0000030 - abnormal mitotic chromosome congression

References:

Genotypes:

FYPO:0000326 - abnormal mitotic sister chromatid biorientation

References:

Genotypes:

FYPO:0000141 - abnormal mitotic sister chromatid segregation

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Genotypes:

FYPO:0001894 - abnormal sporulation resulting in formation of ascus with more or fewer than four spores

References:

Genotypes:

FYPO:0005719 - abolished mitotic metaphase chromosome recapture

References:

Genotypes:

FYPO:0001407 - decreased cell population growth on glucose carbon source

References:

Genotypes:

FYPO:0001919 - fragmented nucleus during vegetative growth

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Genotypes:

FYPO:0000316 - inviable after spore germination

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Genotypes:

FYPO:0002150 - inviable spore population

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Genotypes:

FYPO:0002061 - inviable vegetative cell population

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Genotypes:

FYPO:0000228 - lagging mitotic chromosomes

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Genotypes:

FYPO:0000284 - large and small daughter nuclei, with unequal mitotic sister chromatid segregation

References:

Genotypes:

FYPO:0001164 - normal growth on glucose carbon source

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Genotypes:

FYPO:0000964 - normal growth on thiabendazole

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Genotypes:

FYPO:0006259 - normal mitotic spindle length during metaphase

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Genotypes:

FYPO:0006917 - normal onset of mitotic metaphase/anaphase transition

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Genotypes:

FYPO:0000069 - resistance to thiabendazole

References:

Genotypes:

FYPO:0005634 - sister kinetochore dissociation in meiotic metaphase I with equational sister chromatid segregation in meiosis I

References:

Genotypes:

FYPO:0003241 - unequal mitotic sister chromatid segregation

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Genotypes:

FYPO:0002060 - viable vegetative cell population

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Genotypes:

Qualitative gene expression

PomGeneEx:0000011 - RNA level increased

References:

Quantitative gene expression

PBO:0006310 - protein level

References:

PBO:0011963 - RNA level

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Single locus phenotype

FYPO:0004022 - abnormal cell cycle arrest in mitotic metaphase with long polar microtubules

References:

Genotypes:

FYPO:0004159 - abnormal homologous chromosome segregation

References:

Genotypes:

FYPO:0000030 - abnormal mitotic chromosome congression

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Genotypes:

FYPO:0005362 - abnormal mitotic metaphase chromosome recapture

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Genotypes:

FYPO:0008088 - abolished chromosome oscillation at meiotic metaphase I

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Genotypes:

FYPO:0008099 - abolished homologous chromosome movement at anaphase A

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Genotypes:

FYPO:0005365 - abolished kinetochore sliding during chromosome recapture

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Genotypes:

FYPO:0005366 - abolished protein phosphorylation during mitosis

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Genotypes:

FYPO:0001270 - complete but unequal mitotic sister chromatid segregation

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Genotypes:

FYPO:0003165 - cut with abnormal chromosome segregation

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Genotypes:

FYPO:0003743 - decreased cell population growth during glucose starvation

References:

Genotypes:

FYPO:0001407 - decreased cell population growth on glucose carbon source

References:

Genotypes:

FYPO:0005423 - decreased mitotic spindle microtubule depolymerization during mitotic anaphase A

References:

Genotypes:

FYPO:0007112 - decreased rate of microtubule depolymerization involved in meiotic centromere clustering during meiotic prophase I

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Genotypes:

FYPO:0002638 - increased activation of mitotic spindle assembly checkpoint

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Genotypes:

FYPO:0004023 - increased duration of protein localization to mitotic spindle

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Genotypes:

FYPO:0003003 - increased protein localization to kinetochore during vegetative growth

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Genotypes:

FYPO:0000228 - lagging mitotic chromosomes

References:

Genotypes:

FYPO:0004101 - lagging mitotic chromosomes, with complete sister chromatid separation

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Genotypes:

FYPO:0000733 - long mitotic spindle

References:

Genotypes:

FYPO:0004307 - long mitotic spindle during metaphase

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Genotypes:

FYPO:0001387 - loss of viability at high temperature

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Genotypes:

FYPO:0006518 - loss of viability in G0

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Genotypes:

FYPO:0000283 - mitotic chromosome fragmentation upon segregation

References:

Genotypes:

FYPO:0000324 - mitotic metaphase/anaphase transition delay

References:

Genotypes:

FYPO:0002688 - mitotic spindle collapse without elongation during prophase

References:

Genotypes:

FYPO:0007553 - normal G1 to G0 transition

References:

Genotypes:

FYPO:0001164 - normal growth on glucose carbon source

References:

Genotypes:

FYPO:0005720 - normal mitotic metaphase chromosome recapture

References:

Genotypes:

FYPO:0003627 - normal protein localization

References:

Genotypes:

FYPO:0004214 - normal protein localization to kinetochore during meiosis I

References:

Genotypes:

FYPO:0002901 - normal protein localization to kinetochore during vegetative growth

References:

Genotypes:

FYPO:0004082 - normal protein phosphorylation during mitosis

References:

Genotypes:

FYPO:0000703 - normal protein-protein interaction

References:

Genotypes:

FYPO:0000943 - normal spore morphology

References:

Genotypes:

FYPO:0009031 - resistance to bleomycin

References:

Genotypes:

FYPO:0000764 - resistance to cycloheximide

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Genotypes:

FYPO:0002693 - resistance to diamide

References:

Genotypes:

FYPO:0009038 - resistance to egtazic acid

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Genotypes:

FYPO:0001453 - resistance to ethanol

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Genotypes:

FYPO:0009087 - resistance to magnesium chloride and sodium dodecyl sulfate

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Genotypes:

FYPO:0009043 - resistance to potassium chloride and sodium dodecyl sulfate

References:

Genotypes:

FYPO:0003383 - resistance to tert-butyl hydroperoxide

References:

Genotypes:

FYPO:0000069 - resistance to thiabendazole

References:

Genotypes:

FYPO:0000096 - sensitive to cadmium

References:

Genotypes:

FYPO:0000088 - sensitive to hydroxyurea

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Genotypes:

FYPO:0000270 - sensitive to osmotic stress

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Genotypes:

FYPO:0001214 - sensitive to potassium chloride

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Genotypes:

FYPO:0000091 - sensitive to thiabendazole

References:

Genotypes:

FYPO:0003305 - separate mitotic half spindles present

References:

Genotypes:

FYPO:0005634 - sister kinetochore dissociation in meiotic metaphase I with equational sister chromatid segregation in meiosis I

References:

Genotypes:

FYPO:0000678 - unequal homologous chromosome segregation

References:

Genotypes:

FYPO:0003241 - unequal mitotic sister chromatid segregation

References:

Genotypes:

FYPO:0001491 - viable vegetative cell

References:

Genotypes:

FYPO:0002060 - viable vegetative cell population

References:

Genotypes:

FYPO:0002177 - viable vegetative cell with normal cell morphology

References:

Genotypes:

Taxonomic conservation

PBO:0011065 - conserved in eukaryotes

PBO:0011064 - conserved in fungi

PBO:0011063 - conserved in fungi only

PBO:0006222 - predominantly single copy (one to one)

Protein features

IDNameInterPro nameDB name
PF08653DASH_Dam1DASH_Dam1Pfam
PTHR28113DASH_Dam1PANTHER

Orthologs

References / Literature

PMID:18029449 - In search of an optimal ring to couple microtubule depolymerization to processive chromosome motions.
Efremov A et al. Proc Natl Acad Sci U S A 2007 Nov 27;104(48):19017-22
PMID:16823372 - ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe.
Matsuyama A et al. Nat Biotechnol 2006 Jul;24(7):841-7
PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.
Hayles J et al. Open Biol 2013 May 22;3(5):130053
PMID:24463365 - Systematic screen for mutants resistant to TORC1 inhibition in fission yeast reveals genes involved in cellular ageing and growth.
Rallis C et al. Biol Open 2014 Feb 15;3(2):161-71
PMID:32327557 - Kinesin-14 family proteins and microtubule dynamics define S. pombe mitotic and meiotic spindle assembly, and elongation.
Loncar A et al. J Cell Sci 2020 Jun 08;133(11)
PMID:26537787 - Targeting of SUMO substrates to a Cdc48-Ufd1-Npl4 segregase and STUbL pathway in fission yeast.
Køhler JB et al. Nat Commun 2015 Nov 05;6:8827
PMID:29432178 - General amino acid control in fission yeast is regulated by a nonconserved transcription factor, with functions analogous to Gcn4/Atf4.
Duncan CDS et al. Proc Natl Acad Sci U S A 2018 Feb 20;115(8):E1829-E1838
PMID:22521786 - Phosphodependent recruitment of Bub1 and Bub3 to Spc7/KNL1 by Mph1 kinase maintains the spindle checkpoint.
Shepperd LA et al. Curr Biol 2012 May 22;22(10):891-9
PMID:23166349 - Csi1 links centromeres to the nuclear envelope for centromere clustering.
Hou H et al. J Cell Biol 2012 Nov 26;199(5):735-44
PMID:18262494 - Fission yeast dam1-A8 mutant is resistant to and rescued by an anti-microtubule agent.
Griffiths K et al. Biochem Biophys Res Commun 2008 Apr 11;368(3):670-6
PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.
Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623
GO_REF:0000024 - Manual transfer of experimentally-verified manual GO annotation data to orthologs by curator judgment of sequence similarity.
PMID:26771498 - A Proteome-wide Fission Yeast Interactome Reveals Network Evolution Principles from Yeasts to Human.
Vo TV et al. Cell 2016 Jan 14;164(1-2):310-323
PMID:16079914 - Molecular analysis of kinetochore architecture in fission yeast.
Liu X et al. EMBO J 2005 Aug 17;24(16):2919-30
PMID:33260998 - High-Throughput Flow Cytometry Combined with Genetic Analysis Brings New Insights into the Understanding of Chromatin Regulation of Cellular Quiescence.
Zahedi Y et al. Int J Mol Sci 2020 Nov 27;21(23)
PMID:16537923 - Sterol regulatory element binding protein is a principal regulator of anaerobic gene expression in fission yeast.
Todd BL et al. Mol Cell Biol 2006 Apr;26(7):2817-31
PMID:30824696 - Systematic analysis reveals the prevalence and principles of bypassable gene essentiality.
Li J et al. Nat Commun 2019 Mar 01;10(1):1002
PMID:17035632 - Reconstruction of the kinetochore during meiosis in fission yeast Schizosaccharomyces pombe.
Hayashi A et al. Mol Biol Cell 2006 Dec;17(12):5173-84
PMID:22084306 - CENP-A exceeds microtubule attachment sites in centromere clusters of both budding and fission yeast.
Coffman VC et al. J Cell Biol 2011 Nov 14;195(4):563-72
PMID:24239120 - Antagonistic spindle motors and MAPs regulate metaphase spindle length and chromosome segregation.
Syrovatkina V et al. Curr Biol 2013 Dec 02;23(23):2423-9
PMID:36108046 - Recovery from spindle checkpoint-mediated arrest requires a novel Dnt1-dependent APC/C activation mechanism.
Bai S et al. PLoS Genet 2022 Sep;18(9):e1010397
PMID:20624975 - A non-ring-like form of the Dam1 complex modulates microtubule dynamics in fission yeast.
Gao Q et al. Proc Natl Acad Sci U S A 2010 Jul 27;107(30):13330-5
PMID:23695164 - Cross-species protein interactome mapping reveals species-specific wiring of stress response pathways.
Das J et al. Sci Signal 2013 May 21;6(276):ra38
PMID:18256284 - Sister kinetochore recapture in fission yeast occurs by two distinct mechanisms, both requiring Dam1 and Klp2.
Gachet Y et al. Mol Biol Cell 2008 Apr;19(4):1646-62
PMID:21511999 - Comparative functional genomics of the fission yeasts.
Rhind N et al. Science 2011 May 20;332(6032):930-6
GO_REF:0000111 - Gene Ontology annotations Inferred by Curator (IC) using at least one Inferred by Sequence Similarity (ISS) annotation to support the inference
PMID:36705602 - Unraveling the kinetochore nanostructure in Schizosaccharomyces pombe using multi-color SMLM imaging.
Virant D et al. J Cell Biol 2023 Apr 03;222(4)
PMID:23770679 - Microtubules and Alp7-Alp14 (TACC-TOG) reposition chromosomes before meiotic segregation.
Kakui Y et al. Nat Cell Biol 2013 Jul;15(7):786-96
PMID:33529549 - Chiasmata and the kinetochore component Dam1 are crucial for elimination of erroneous chromosome attachments and centromere oscillation at meiosis I.
Wakiya M et al. Open Biol 2021 Feb;11(2):200308
PMID:37787768 - Broad functional profiling of fission yeast proteins using phenomics and machine learning.
Rodríguez-López M et al. Elife 2023 Oct 03;12
PMID:22681890 - Hierarchical modularity and the evolution of genetic interactomes across species.
Ryan CJ et al. Mol Cell 2012 Jun 08;46(5):691-704
PMID:18684775 - A genome-wide screen of genes involved in cadmium tolerance in Schizosaccharomyces pombe.
Kennedy PJ et al. Toxicol Sci 2008 Nov;106(1):124-39
PMID:21256022 - Ndc80 internal loop interacts with Dis1/TOG to ensure proper kinetochore-spindle attachment in fission yeast.
Hsu KS et al. Curr Biol 2011 Feb 08;21(3):214-20
PMID:22235339 - The S. pombe histone H2A dioxygenase Ofd2 regulates gene expression during hypoxia.
Lando D et al. PLoS One 2012;7(1):e29765
PMID:22375062 - Plo1 phosphorylates Dam1 to promote chromosome bi-orientation in fission yeast.
Buttrick GJ et al. J Cell Sci 2012 Apr 01;125(Pt 7):1645-51
PMID:16079915 - The DASH complex and Klp5/Klp6 kinesin coordinate bipolar chromosome attachment in fission yeast.
Sanchez-Perez I et al. EMBO J 2005 Aug 17;24(16):2931-43
PMID:17881496 - The Dam1/DASH complex is required for the retrieval of unclustered kinetochores in fission yeast.
Franco A et al. J Cell Sci 2007 Oct 01;120(Pt 19):3345-51
PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.
Marguerat S et al. Cell 2012 Oct 26;151(3):671-83