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protein coding gene - cwh43 (SPAC589.12) - glycosylceramide biosynthesis protein Cwh43

Gene summary

Standard name
cwh43
Systematic ID
SPAC589.12
Product
glycosylceramide biosynthesis protein Cwh43
Organism
Schizosaccharomyces pombe (fission yeast)
Synonyms
SPAC688.01
UniProt ID
Q9HDZ2
ORFeome ID
49/49F09
Characterisation status
biological role inferred
Feature type
mRNA gene
Genomic location
chromosome I: 3110201..3113396 forward strand

Annotation

GO biological process

GO:0031505 - fungal-type cell wall organization

References:

GO:0006506 - GPI anchor biosynthetic process

References:

GO cellular component

GO:0005783 - endoplasmic reticulum

References:

GO:0005789 - endoplasmic reticulum membrane

References:

GO:0005635 - nuclear envelope

References:

GO:0005886 - plasma membrane

References:

Modification

MOD:00048 - O4'-phospho-L-tyrosine

References:

Multi-locus phenotype

FYPO:0000082 - decreased cell population growth at high temperature

References:

Genotypes:

FYPO:0006054 - decreased lipid droplet formation

References:

Genotypes:

FYPO:0001355 - decreased vegetative cell population growth

References:

Genotypes:

FYPO:0002061 - inviable vegetative cell population

References:

Genotypes:

FYPO:0006660 - loss of viability upon G0 to G1 transition

References:

Genotypes:

FYPO:0002048 - normal cell morphology during nitrogen starvation

References:

Genotypes:

FYPO:0006579 - sensitive to manganese

References:

Genotypes:

FYPO:0001234 - slow vegetative cell population growth

References:

Genotypes:

FYPO:0002903 - viable pear-shaped vegetative cell

References:

Genotypes:

FYPO:0002060 - viable vegetative cell population

References:

Genotypes:

Protein sequence feature

SO:0001812 - transmembrane_helix

References:

Qualitative gene expression

PomGeneEx:0000018 - protein level increased

References:

PomGeneEx:0000011 - RNA level increased

References:

Quantitative gene expression

PBO:0006310 - protein level

References:

PBO:0011963 - RNA level

References:

Single locus phenotype

FYPO:0000031 - abnormal mating

References:

Genotypes:

FYPO:0000357 - abnormal shmoo morphology

References:

Genotypes:

FYPO:0001118 - abnormal vegetative cell morphology

References:

Genotypes:

FYPO:0002196 - abnormal vegetative cell shape

References:

Genotypes:

FYPO:0004481 - abolished cell population growth at high temperature

References:

Genotypes:

FYPO:0000417 - abolished cytokinesis

References:

Genotypes:

FYPO:0006010 - abolished shmoo contact with partner cell

References:

Genotypes:

FYPO:0000082 - decreased cell population growth at high temperature

References:

Genotypes:

FYPO:0003743 - decreased cell population growth during glucose starvation

References:

Genotypes:

FYPO:0006967 - decreased cellular AICAR level

References:

Genotypes:

FYPO:0006971 - decreased cellular biotin level

References:

Genotypes:

FYPO:0006970 - decreased cellular bisphosphoglyceric acid level

References:

Genotypes:

FYPO:0006700 - decreased cellular CDP level

References:

Genotypes:

FYPO:0006969 - decreased cellular cytidine level

References:

Genotypes:

FYPO:0006973 - decreased cellular L-glutamate methyl ester level

References:

Genotypes:

FYPO:0006972 - decreased cellular S-adenosyl-L-cysteine level

References:

Genotypes:

FYPO:0006696 - decreased cellular S-adenosyl-L-homocysteine level during vegetative growth

References:

Genotypes:

FYPO:0006968 - decreased cellular SAICAR level

References:

Genotypes:

FYPO:0000708 - decreased mating efficiency

References:

Genotypes:

FYPO:0000419 - decreased rate of cytokinesis

References:

Genotypes:

FYPO:0000998 - elongated cell during nitrogen starvation

References:

Genotypes:

FYPO:0004860 - increased cell wall beta-glucan level

References:

Genotypes:

FYPO:0006963 - increased cellular 1-methyladenosine level

References:

Genotypes:

FYPO:0006958 - increased cellular 2-oxoglutarate level

References:

Genotypes:

FYPO:0006966 - increased cellular acetyl-CoA level

References:

Genotypes:

FYPO:0006960 - increased cellular adenosine level

References:

Genotypes:

FYPO:0006953 - increased cellular asparagine level

References:

Genotypes:

FYPO:0006964 - increased cellular coenzyme A level

References:

Genotypes:

FYPO:0006956 - increased cellular deferrichrome level

References:

Genotypes:

FYPO:0005729 - increased cellular ferrichrome level

References:

Genotypes:

FYPO:0006955 - increased cellular glutamine level

References:

Genotypes:

FYPO:0003518 - increased cellular hercynine level during vegetative growth

References:

Genotypes:

FYPO:0003519 - increased cellular hercynylcysteine sulfoxide level during vegetative growth

References:

Genotypes:

FYPO:0006957 - increased cellular histidinol level

References:

Genotypes:

FYPO:0006965 - increased cellular HMG-CoA level

References:

Genotypes:

FYPO:0006961 - increased cellular inosine level

References:

Genotypes:

FYPO:0006954 - increased cellular isoleucine level

References:

Genotypes:

FYPO:0003516 - increased cellular L-ergothioneine level during vegetative growth

References:

Genotypes:

FYPO:0006950 - increased cellular myo-inositol level

References:

Genotypes:

FYPO:0006951 - increased cellular N(alpha),N(alpha)-dimethyl-L-histidine level

References:

Genotypes:

FYPO:0006952 - increased cellular N2-acetyl-L-lysine level

References:

Genotypes:

FYPO:0005237 - increased cellular proline level

References:

Genotypes:

FYPO:0006949 - increased cellular S-methyl-L-ergothioneine level during vegetative growth

References:

Genotypes:

FYPO:0002327 - increased cellular sterol level

References:

Genotypes:

FYPO:0006959 - increased cellular succinate level

References:

Genotypes:

FYPO:0002658 - increased cellular trehalose 6-phosphate level

References:

Genotypes:

FYPO:0001444 - increased cellular trehalose level during vegetative growth

References:

Genotypes:

FYPO:0002227 - increased cellular triglyceride level during vegetative growth

References:

Genotypes:

FYPO:0006962 - increased cellular UDP-N-acetylglucosamine level

References:

Genotypes:

FYPO:0000651 - increased duration of septum assembly

References:

Genotypes:

FYPO:0006945 - increased lipid droplet formation

References:

Genotypes:

FYPO:0000650 - increased septation index

References:

Genotypes:

FYPO:0002251 - inviable swollen elongated vegetative cell

References:

Genotypes:

FYPO:0002414 - inviable swollen vegetative cell

References:

Genotypes:

FYPO:0006660 - loss of viability upon G0 to G1 transition

References:

Genotypes:

FYPO:0001178 - loss of viability upon nitrogen starvation

References:

Genotypes:

FYPO:0002002 - multiseptate vegetative cell, septa grouped

References:

Genotypes:

FYPO:0004165 - normal glucose consumption

References:

Genotypes:

FYPO:0001020 - normal growth on calcium

References:

Genotypes:

FYPO:0006948 - normal protein level during glucose starvation

References:

Genotypes:

FYPO:0004887 - normal protein localization to nuclear envelope during vegetative growth

References:

Genotypes:

FYPO:0002674 - normal protein localization to plasma membrane

References:

Genotypes:

FYPO:0006947 - protein mislocalized to cytoplasm during glucose starvation

References:

Genotypes:

FYPO:0000067 - resistance to brefeldin A

References:

Genotypes:

FYPO:0006933 - resistance to butylated hydroxytoluene

References:

Genotypes:

FYPO:0000763 - resistance to cadmium

References:

Genotypes:

FYPO:0000764 - resistance to cycloheximide

References:

Genotypes:

FYPO:0002693 - resistance to diamide

References:

Genotypes:

FYPO:0009038 - resistance to egtazic acid

References:

Genotypes:

FYPO:0009085 - resistance to lithium chloride and sodium dodecyl sulfate

References:

Genotypes:

FYPO:0009043 - resistance to potassium chloride and sodium dodecyl sulfate

References:

Genotypes:

FYPO:0006579 - sensitive to manganese

References:

Genotypes:

FYPO:0003877 - single cell division prior to G0 entry

References:

Genotypes:

FYPO:0001234 - slow vegetative cell population growth

References:

Genotypes:

FYPO:0000646 - swollen vegetative cell

References:

Genotypes:

FYPO:0006974 - triglyceride absent from cell

References:

Genotypes:

FYPO:0002380 - viable spheroid vegetative cell

References:

Genotypes:

FYPO:0002060 - viable vegetative cell population

References:

Genotypes:

Taxonomic conservation

PBO:0011065 - conserved in eukaryotes

PBO:0011071 - conserved in eukaryotes only

PBO:0011064 - conserved in fungi

PBO:0011069 - conserved in metazoa

PBO:0011070 - conserved in vertebrates

PBO:0006222 - predominantly single copy (one to one)

Protein features

IDNameInterPro nameDB name
PF10277Frag1CWH43_NPfam
PF230216TM_2nd_PGAP2IPPGAP2IP_TM_2ndPfam
PF230226TM_1st_PGAP2IPPGAP2IP_TM_1ndPfam
PF23226Exo_endo_phos_PGAP2IPExo_endo_phos_PGAP2IP_CPfam
G3DSA:3.60.10.10:FF:000100CATH-FunFam
G3DSA:3.60.10.10Endo/exonu/phosph_ase_sfCATH-Gene3D
SSF56219Endo/exonu/phosph_ase_sfSUPERFAMILY
PTHR14859GPI-anchor_lipid_remodelerPANTHER
Transmembrane alpha helixDeepTMHMM

Orthologs

References / Literature

PMID:37787768 - Broad functional profiling of fission yeast proteins using phenomics and machine learning.
Rodríguez-López M et al. Elife 2023 Oct 03;12
PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.
Hayles J et al. Open Biol 2013 May 22;3(5):130053
PMID:30726745 - Fission Yeast NDR/LATS Kinase Orb6 Regulates Exocytosis via Phosphorylation of the Exocyst Complex.
Tay YD et al. Cell Rep 2019 Feb 05;26(6):1654-1667.e7
PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.
Marguerat S et al. Cell 2012 Oct 26;151(3):671-83
PMID:28410370 - A systematic screen for morphological abnormalities during fission yeast sexual reproduction identifies a mechanism of actin aster formation for cell fusion.
Dudin O et al. PLoS Genet 2017 Apr;13(4):e1006721
PMID:39367033 - Quantitative proteomics and phosphoproteomics profiling of meiotic divisions in the fission yeast Schizosaccharomyces pombe.
Sivakova B et al. Sci Rep 2024 Oct 04;14(1):23105
PMID:40015273 - A comprehensive Schizosaccharomyces pombe atlas of physical transcription factor interactions with proteins and chromatin.
Skribbe M et al. Mol Cell 2025 Feb 19;
PMID:24763107 - Absolute proteome and phosphoproteome dynamics during the cell cycle of Schizosaccharomyces pombe (Fission Yeast).
Carpy A et al. Mol Cell Proteomics 2014 Aug;13(8):1925-36
PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.
Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623
PMID:20519959 - S. pombe genome deletion project: an update.
Spirek M et al. Cell Cycle 2010 Jun 15;9(12):2399-402
PMID:16823372 - ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe.
Matsuyama A et al. Nat Biotechnol 2006 Jul;24(7):841-7
PMID:26771498 - A Proteome-wide Fission Yeast Interactome Reveals Network Evolution Principles from Yeasts to Human.
Vo TV et al. Cell 2016 Jan 14;164(1-2):310-323
PMID:30072439 - The putative ceramide-conjugation protein Cwh43 regulates G0 quiescence, nutrient metabolism and lipid homeostasis in fission yeast.
Nakazawa N et al. J Cell Sci 2018 Aug 21;131(16)
PMID:30647105 - Comparative Genomic Screen in Two Yeasts Reveals Conserved Pathways in the Response Network to Phenol Stress.
Alhoch B et al. G3 (Bethesda) 2019 Mar 07;9(3):639-650
PMID:12161753 - The transcriptional program of meiosis and sporulation in fission yeast.
Mata J et al. Nat Genet 2002 Sep;32(1):143-7
PMID:29259000 - Genes Important for Schizosaccharomyces pombe Meiosis Identified Through a Functional Genomics Screen.
Blyth J et al. Genetics 2018 Feb;208(2):589-603
PMID:31201205 - Coordinated Roles of the Putative Ceramide-Conjugation Protein, Cwh43, and a Mn 2+ -Transporting, P-Type ATPase, Pmr1, in Fission Yeast.
Nakazawa N et al. G3 (Bethesda) 2019 Aug 08;9(8):2667-2676
GO_REF:0000033 - Annotation inferences using phylogenetic trees
PMID:11152613 - Predicting transmembrane protein topology with a hidden Markov model: application to complete genomes.
Krogh A et al. J Mol Biol 2001 Jan 19;305(3):567-80
PMID:21511999 - Comparative functional genomics of the fission yeasts.
Rhind N et al. Science 2011 May 20;332(6032):930-6
PMID:36799444 - Inner nuclear membrane proteins Lem2 and Bqt4 interact with different lipid synthesis enzymes in fission yeast.
Hirano Y et al. J Biochem 2023 Jun 30;174(1):33-46
PMID:28974540 - The fission yeast nucleoporin Alm1 is required for proteasomal degradation of kinetochore components.
Salas-Pino S et al. J Cell Biol 2017 Nov 06;216(11):3591-3608
PMID:16537923 - Sterol regulatory element binding protein is a principal regulator of anaerobic gene expression in fission yeast.
Todd BL et al. Mol Cell Biol 2006 Apr;26(7):2817-31