protein coding gene - ecm31 (SPAC5H10.09c) - 3-methyl-2-oxobutanoatehydroxymethyltransferase Ecm31

Gene summary

Standard name: ecm31
Systematic ID: SPAC5H10.09c
Product: 3-methyl-2-oxobutanoatehydroxymethyltransferase Ecm31
Organism: Schizosaccharomyces pombe (fission yeast)
UniProt ID: Q09672
ORFeome ID: 10/10B09
Characterisation status: biological role inferred
Feature type: mRNA gene
Genomic location: chromosome I: 160977..161816 reverse strand

Annotation

GO biological process

GO:0015940 - pantothenate biosynthetic process

References:

GO_REF:0000033

GO cellular component

GO:0005739 - mitochondrion

References:

PMID:16823372

GO molecular function

GO:0003864 - 3-methyl-2-oxobutanoate hydroxymethyltransferase activity

References:

GO_REF:0000033

GO:0000287 - magnesium ion binding

References:

GO_REF:0000033

Qualitative gene expression

PomGeneEx:0000012 - RNA level decreased

References:

PMID:27984744

Quantitative gene expression

PBO:0006310 - protein level

References:

PBO:0011963 - RNA level

References:

PMID:23101633

Single locus phenotype

FYPO:0003625 - abnormal microtubule cytoskeleton morphology during mitotic interphase

References:

PMID:25373780

Genotypes:

ecm31delta

FYPO:0000764 - resistance to cycloheximide

References:

PMID:37787768

Genotypes:

ecm31delta

FYPO:0009038 - resistance to egtazic acid

References:

PMID:37787768

Genotypes:

ecm31delta

FYPO:0009085 - resistance to lithium chloride and sodium dodecyl sulfate

References:

PMID:37787768

Genotypes:

ecm31delta

FYPO:0009043 - resistance to potassium chloride and sodium dodecyl sulfate

References:

PMID:37787768

Genotypes:

ecm31delta

FYPO:0003383 - resistance to tert-butyl hydroperoxide

References:

PMID:37787768

Genotypes:

ecm31delta

FYPO:0001034 - resistance to tunicamycin

References:

PMID:37787768

Genotypes:

ecm31delta

FYPO:0002060 - viable vegetative cell population

References:

Genotypes:

ecm31delta

FYPO:0002177 - viable vegetative cell with normal cell morphology

References:

PMID:23697806

Genotypes:

ecm31delta

Taxonomic conservation

PBO:0011067 - conserved in bacteria

PBO:0011065 - conserved in eukaryotes

PBO:0011064 - conserved in fungi

Warnings

PBO:0000164 - co-expressed with adjacent gene

PBO:0000085 - possible horizontal transfer

References:

PMID:21511999

Protein features

ID	Name	InterPro name	DB name
PF02548	Pantoate_transf	Pantoate_hydroxy_MeTrfase	PFAM
cd06557	KPHMT-like	Pantoate_hydroxy_MeTrfase	CDD
G3DSA:3.20.20.60:FF:000003			FUNFAM
SSF51621	Phosphoenolpyruvate/pyruvate domain	Pyrv/PenolPyrv_kinase-like_dom	SUPERFAMILY
G3DSA:3.20.20.60		Pyrv_kinase-like_dom_sf	GENE3D
PTHR20881	3-METHYL-2-OXOBUTANOATE HYDROXYMETHYLTRANSFERASE	Pantoate_hydroxy_MeTrfase	PANTHER
PIRSF000388	PanB	Pantoate_hydroxy_MeTrfase	PIRSF
MF_00156	PanB	Pantoate_hydroxy_MeTrfase	HAMAP
TIGR00222	panB	Pantoate_hydroxy_MeTrfase	NCBIFAM
NF001452	PRK00311.1		NCBIFAM

Orthologs

ecm31 (SJAG_05200) Schizosaccharomyces japonicus
ECM31 (YBR176W) Saccharomyces cerevisiae

References / Literature

PMID:21511999 - Comparative functional genomics of the fission yeasts.: Rhind N et al. Science 2011 May 20;332(6032):930-6
PMID:26771498 - A Proteome-wide Fission Yeast Interactome Reveals Network Evolution Principles from Yeasts to Human.: Vo TV et al. Cell 2016 Jan 14;164(1-2):310-323
PMID:41526646 - Development of a global screening system for detecting protein-protein interactions by luminescence complementation in fission yeast.: Azadeh F et al. Sci Rep 2026 Jan 12;
PMID:27984744 - Survival in Quiescence Requires the Euchromatic Deployment of Clr4/SUV39H by Argonaute-Associated Small RNAs.: Joh RI et al. Mol Cell 2016 Dec 15;64(6):1088-1101
PMID:23695164 - Cross-species protein interactome mapping reveals species-specific wiring of stress response pathways.: Das J et al. Sci Signal 2013 May 21;6(276):ra38
PMID:16823372 - ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe.: Matsuyama A et al. Nat Biotechnol 2006 Jul;24(7):841-7
PMID:22681890 - Hierarchical modularity and the evolution of genetic interactomes across species.: Ryan CJ et al. Mol Cell 2012 Jun 08;46(5):691-704
PMID:25373780 - A genomic Multiprocess survey of machineries that control and link cell shape, microtubule organization, and cell-cycle progression.: Graml V et al. Dev Cell 2014 Oct 27;31(2):227-239
PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.: Hayles J et al. Open Biol 2013 May 22;3(5):130053
PMID:24763107 - Absolute proteome and phosphoproteome dynamics during the cell cycle of Schizosaccharomyces pombe (Fission Yeast).: Carpy A et al. Mol Cell Proteomics 2014 Aug;13(8):1925-36
PMID:23050226 - A genetic screen to discover pathways affecting cohesin function in Schizosaccharomyces pombe identifies chromatin effectors.: Chen Z et al. G3 (Bethesda) 2012 Oct;2(10):1161-8
PMID:24957674 - Yeast X-chromosome-associated protein 5 (Xap5) functions with H2A.Z to suppress aberrant transcripts.: Anver S et al. EMBO Rep 2014 Aug;15(8):894-902
PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.: Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623
GO_REF:0000033 - Annotation inferences using phylogenetic trees
PMID:37787768 - Broad functional profiling of fission yeast proteins using phenomics and machine learning.: Rodríguez-López M et al. Elife 2023 Oct 03;12
PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.: Marguerat S et al. Cell 2012 Oct 26;151(3):671-83