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protein coding gene - gmh1 (SPAC5H10.11) - alpha-1,2-galactosyltransferase Gmh1

Gene summary

Standard name
gmh1
Systematic ID
SPAC5H10.11
Product
alpha-1,2-galactosyltransferase Gmh1
Organism
Schizosaccharomyces pombe (fission yeast)
UniProt ID
Q09679
ORFeome ID
43/43F10
Characterisation status
biological role published
Feature type
mRNA gene
Genomic location
chromosome I: 164728..166335 forward strand

Annotation

GO biological process

GO:0006487 - protein N-linked glycosylation

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GO cellular component

GO:0005783 - endoplasmic reticulum

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GO:0005794 - Golgi apparatus

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GO:0000139 - Golgi membrane

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GO molecular function

GO:0031278 - alpha-1,2-galactosyltransferase activity

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Modification

MOD:00006 - N-glycosylated residue

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Multi-locus phenotype

FYPO:0006277 - abnormal N-linked glycoprotein glycan structure

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Genotypes:

FYPO:0006278 - abnormal O-linked glycoprotein glycan structure

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Genotypes:

FYPO:0003354 - decreased galactose level in glycoprotein glycan

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Genotypes:

FYPO:0001355 - decreased vegetative cell population growth

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Genotypes:

FYPO:0003291 - galactose absent from glycoprotein glycan

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Genotypes:

FYPO:0002061 - inviable vegetative cell population

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Genotypes:

FYPO:0001120 - pear-shaped vegetative cell

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Genotypes:

FYPO:0000106 - sensitive to hygromycin B

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Genotypes:

FYPO:0000021 - spheroid vegetative cell

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Genotypes:

Protein sequence feature

SO:0000418 - signal_peptide

SO:0001812 - transmembrane_helix

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Qualitative gene expression

PomGeneEx:0000018 - protein level increased

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PomGeneEx:0000011 - RNA level increased

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Quantitative gene expression

PBO:0011963 - RNA level

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Single locus phenotype

FYPO:0001357 - normal vegetative cell population growth

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Genotypes:

FYPO:0002693 - resistance to diamide

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Genotypes:

FYPO:0009038 - resistance to egtazic acid

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Genotypes:

FYPO:0009043 - resistance to potassium chloride and sodium dodecyl sulfate

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Genotypes:

FYPO:0000096 - sensitive to cadmium

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Genotypes:

FYPO:0002060 - viable vegetative cell population

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Genotypes:

FYPO:0002177 - viable vegetative cell with normal cell morphology

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Genotypes:

Taxonomic conservation

PBO:0011065 - conserved in eukaryotes

PBO:0011071 - conserved in eukaryotes only

PBO:0011064 - conserved in fungi

Protein features

IDNameInterPro nameDB name
PF05637Glyco_transf_34Glyco_trans_34Pfam
G3DSA:3.90.550.10:FF:000149CATH-FunFam
G3DSA:3.90.550.10Nucleotide-diphossugar_transCATH-Gene3D
PTHR31306Glyco_trans_34PANTHER
Transmembrane alpha helixDeepTMHMM

Orthologs

References / Literature

PMID:21098516 - Structural analysis of α1,3-linked galactose-containing oligosaccharides in Schizosaccharomyces pombe mutants harboring single and multiple α-galactosyltransferase genes disruptions.
Ohashi T et al. Glycobiology 2011 Mar;21(3):340-51
PMID:29432178 - General amino acid control in fission yeast is regulated by a nonconserved transcription factor, with functions analogous to Gcn4/Atf4.
Duncan CDS et al. Proc Natl Acad Sci U S A 2018 Feb 20;115(8):E1829-E1838
PMID:22988247 - Identification of novel α1,3-galactosyltransferase and elimination of α-galactose-containing glycans by disruption of multiple α-galactosyltransferase genes in Schizosaccharomyces pombe.
Ohashi T et al. J Biol Chem 2012 Nov 09;287(46):38866-75
PMID:37787768 - Broad functional profiling of fission yeast proteins using phenomics and machine learning.
Rodríguez-López M et al. Elife 2023 Oct 03;12
PMID:33109728 - High-Throughput Identification of Nuclear Envelope Protein Interactions in Schizosaccharomyces pombe Using an Arrayed Membrane Yeast-Two Hybrid Library.
Varberg JM et al. G3 (Bethesda) 2020 Dec 03;10(12):4649-4663
PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.
Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623
PMID:27558664 - Global Fitness Profiling Identifies Arsenic and Cadmium Tolerance Mechanisms in Fission Yeast.
Guo L et al. G3 (Bethesda) 2016 Oct 13;6(10):3317-3333
PMID:33909078 - Substrate specificities of α1,2- and α1,3-galactosyltransferases and characterization of Gmh1p and Otg1p in Schizosaccharomyces pombe.
Fukunaga T et al. Glycobiology 2021 Sep 09;31(8):1037-1045
PMID:12161753 - The transcriptional program of meiosis and sporulation in fission yeast.
Mata J et al. Nat Genet 2002 Sep;32(1):143-7
PMID:26896847 - Ensembl comparative genomics resources.
Herrero J et al. Database (Oxford) 2016;2016
PMID:39367033 - Quantitative proteomics and phosphoproteomics profiling of meiotic divisions in the fission yeast Schizosaccharomyces pombe.
Sivakova B et al. Sci Rep 2024 Oct 04;14(1):23105
GO_REF:0000033 - Annotation inferences using phylogenetic trees
PMID:36408920 - UniProt: the Universal Protein Knowledgebase in 2023.
UniProt Consortium Nucleic Acids Res 2023 Jan 06;51(D1):D523-D531
PMID:26771498 - A Proteome-wide Fission Yeast Interactome Reveals Network Evolution Principles from Yeasts to Human.
Vo TV et al. Cell 2016 Jan 14;164(1-2):310-323
PMID:33313903 - Ribosome profiling reveals ribosome stalling on tryptophan codons and ribosome queuing upon oxidative stress in fission yeast.
Rubio A et al. Nucleic Acids Res 2021 Jan 11;49(1):383-399
PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.
Hayles J et al. Open Biol 2013 May 22;3(5):130053
PMID:9839953 - Differences in in vivo acceptor specificity of two galactosyltransferases, the gmh3+ and gma12+ gene products from Schizosaccharomyces pombe.
Yoko-o T et al. Eur J Biochem 1998 Nov 01;257(3):630-7
PMID:11152613 - Predicting transmembrane protein topology with a hidden Markov model: application to complete genomes.
Krogh A et al. J Mol Biol 2001 Jan 19;305(3):567-80
PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.
Marguerat S et al. Cell 2012 Oct 26;151(3):671-83
PMID:16823372 - ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe.
Matsuyama A et al. Nat Biotechnol 2006 Jul;24(7):841-7