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protein coding gene - gmh2 (SPAC5H10.13c) - alpha-1,2-galactosyltransferase Gmh2

Gene summary

Standard name
gmh2
Systematic ID
SPAC5H10.13c
Product
alpha-1,2-galactosyltransferase Gmh2
Organism
Schizosaccharomyces pombe (fission yeast)
UniProt ID
Q09681
ORFeome ID
15/15G07
Characterisation status
biological role published
Feature type
mRNA gene
Genomic location
chromosome I: 167766..170055 reverse strand

Annotation

GO biological process

GO:0006487 - protein N-linked glycosylation

References:

GO cellular component

GO:0000139 - Golgi membrane

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GO molecular function

GO:0031278 - alpha-1,2-galactosyltransferase activity

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Modification

MOD:00006 - N-glycosylated residue

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Multi-locus phenotype

FYPO:0006277 - abnormal N-linked glycoprotein glycan structure

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Genotypes:

FYPO:0006278 - abnormal O-linked glycoprotein glycan structure

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Genotypes:

FYPO:0003354 - decreased galactose level in glycoprotein glycan

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Genotypes:

FYPO:0001355 - decreased vegetative cell population growth

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Genotypes:

FYPO:0003291 - galactose absent from glycoprotein glycan

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Genotypes:

FYPO:0002061 - inviable vegetative cell population

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Genotypes:

FYPO:0001120 - pear-shaped vegetative cell

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Genotypes:

FYPO:0000106 - sensitive to hygromycin B

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Genotypes:

FYPO:0000021 - spheroid vegetative cell

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Genotypes:

Protein sequence feature

SO:0000418 - signal_peptide

SO:0001812 - transmembrane_helix

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Qualitative gene expression

PomGeneEx:0000019 - protein level decreased

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PomGeneEx:0000018 - protein level increased

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PomGeneEx:0000012 - RNA level decreased

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Quantitative gene expression

PBO:0006310 - protein level

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PBO:0011963 - RNA level

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Single locus phenotype

FYPO:0003354 - decreased galactose level in glycoprotein glycan

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Genotypes:

FYPO:0009072 - increased cell population growth on lysine nitrogen source

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Genotypes:

FYPO:0001309 - increased viability in stationary phase

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Genotypes:

FYPO:0001357 - normal vegetative cell population growth

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Genotypes:

FYPO:0002578 - resistance to hydroxyurea

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Genotypes:

FYPO:0009070 - resistance to itraconazole

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Genotypes:

FYPO:0000096 - sensitive to cadmium

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Genotypes:

FYPO:0000104 - sensitive to cycloheximide

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Genotypes:

FYPO:0007931 - sensitive to egtazic acid

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Genotypes:

FYPO:0009086 - sensitive to lithium chloride and sodium dodecyl sulfate

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Genotypes:

FYPO:0000797 - sensitive to tert-butyl hydroperoxide

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Genotypes:

FYPO:0002060 - viable vegetative cell population

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Genotypes:

FYPO:0002177 - viable vegetative cell with normal cell morphology

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Genotypes:

Taxonomic conservation

PBO:0011065 - conserved in eukaryotes

PBO:0011071 - conserved in eukaryotes only

PBO:0011064 - conserved in fungi

Protein features

IDNameInterPro nameDB name
PF05637Glyco_transf_34Glyco_trans_34PFAM
G3DSA:3.90.550.10:FF:000149FUNFAM
G3DSA:3.90.550.10Spore Coat Polysaccharide Biosynthesis Protein SpsA; Chain ANucleotide-diphossugar_transGENE3D
PTHR31306ALPHA-1,6-MANNOSYLTRANSFERASE MNN11-RELATEDGlyco_trans_34PANTHER

Orthologs

References / Literature

PMID:33313903 - Ribosome profiling reveals ribosome stalling on tryptophan codons and ribosome queuing upon oxidative stress in fission yeast.
Rubio A et al. Nucleic Acids Res 2021 Jan 11;49(1):383-399
PMID:34250083 - Barcode sequencing and a high-throughput assay for chronological lifespan uncover ageing-associated genes in fission yeast.
Romila CA et al. Microb Cell 2021 Jul 05;8(7):146-160
PMID:39367033 - Quantitative proteomics and phosphoproteomics profiling of meiotic divisions in the fission yeast Schizosaccharomyces pombe.
Sivakova B et al. Sci Rep 2024 Oct 04;14(1):23105
PMID:22681890 - Hierarchical modularity and the evolution of genetic interactomes across species.
Ryan CJ et al. Mol Cell 2012 Jun 08;46(5):691-704
PMID:21098516 - Structural analysis of α1,3-linked galactose-containing oligosaccharides in Schizosaccharomyces pombe mutants harboring single and multiple α-galactosyltransferase genes disruptions.
Ohashi T et al. Glycobiology 2011 Mar;21(3):340-51
PMID:11152613 - Predicting transmembrane protein topology with a hidden Markov model: application to complete genomes.
Krogh A et al. J Mol Biol 2001 Jan 19;305(3):567-80
PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.
Marguerat S et al. Cell 2012 Oct 26;151(3):671-83
PMID:36408920 - UniProt: the Universal Protein Knowledgebase in 2023.
UniProt Consortium Nucleic Acids Res 2023 Jan 06;51(D1):D523-D531
PMID:24763107 - Absolute proteome and phosphoproteome dynamics during the cell cycle of Schizosaccharomyces pombe (Fission Yeast).
Carpy A et al. Mol Cell Proteomics 2014 Aug;13(8):1925-36
PMID:22633491 - Mapping N-glycosylation sites across seven evolutionarily distant species reveals a divergent substrate proteome despite a common core machinery.
Zielinska DF et al. Mol Cell 2012 May 25;46(4):542-8
PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.
Hayles J et al. Open Biol 2013 May 22;3(5):130053
PMID:9839953 - Differences in in vivo acceptor specificity of two galactosyltransferases, the gmh3+ and gma12+ gene products from Schizosaccharomyces pombe.
Yoko-o T et al. Eur J Biochem 1998 Nov 01;257(3):630-7
PMID:39705284 - Proteomic and phosphoproteomic analyses reveal that TORC1 is reactivated by pheromone signaling during sexual reproduction in fission yeast.
Bérard M et al. PLoS Biol 2024 Dec 20;22(12):e3002963
PMID:19547744 - Evolution of phosphoregulation: comparison of phosphorylation patterns across yeast species.
Beltrao P et al. PLoS Biol 2009 Jun 16;7(6):e1000134
PMID:26896847 - Ensembl comparative genomics resources.
Herrero J et al. Database (Oxford) 2016;2016
PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.
Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623
PMID:37787768 - Broad functional profiling of fission yeast proteins using phenomics and machine learning.
Rodríguez-López M et al. Elife 2023 Oct 03;12
PMID:33909078 - Substrate specificities of α1,2- and α1,3-galactosyltransferases and characterization of Gmh1p and Otg1p in Schizosaccharomyces pombe.
Fukunaga T et al. Glycobiology 2021 Sep 09;31(8):1037-1045
PMID:26771498 - A Proteome-wide Fission Yeast Interactome Reveals Network Evolution Principles from Yeasts to Human.
Vo TV et al. Cell 2016 Jan 14;164(1-2):310-323
PMID:22988247 - Identification of novel α1,3-galactosyltransferase and elimination of α-galactose-containing glycans by disruption of multiple α-galactosyltransferase genes in Schizosaccharomyces pombe.
Ohashi T et al. J Biol Chem 2012 Nov 09;287(46):38866-75
GO_REF:0000033 - Annotation inferences using phylogenetic trees