protein coding gene - arp3 (SPAC630.03) - ARP2/3 actin-organizing complex actin-related protein subunit Arp3

Gene summary

Standard name

arp3

Systematic ID

SPAC630.03

Product

ARP2/3 actin-organizing complex actin-related protein subunit Arp3

Organism

Schizosaccharomyces pombe (fission yeast)

Synonyms

act2

UniProt ID

P32390

ORFeome ID

33/33C01

Characterisation status

biological role published

Feature type

mRNA gene

Genomic location

chromosome I: 350433..352296 forward strand

Annotation

Protein features

Orthologs

• ACTR3 (HGNC:170) Homo sapiens
• ACTR3B (HGNC:17256) Homo sapiens
• ACTR3C (HGNC:37282) Homo sapiens
• arp3 (SJAG_01268) Schizosaccharomyces japonicus
• ARP3 (YJR065C) Saccharomyces cerevisiae

References / Literature

PMID:17412958 - A conserved family of enzymes that phosphorylate inositol hexakisphosphate.

Mulugu S et al. Science 2007 Apr 06;316(5821):106-9

PMID:18640983 - Structure and biochemical properties of fission yeast Arp2/3 complex lacking the Arp2 subunit.

Nolen BJ et al. J Biol Chem 2008 Sep 26;283(39):26490-8

PMID:11231572 - Role of actin polymerization and actin cables in actin-patch movement in Schizosaccharomyces pombe.

Pelham RJ et al. Nat Cell Biol 2001 Mar;3(3):235-44

PMID:8978671 - The Schizosaccharomyces pombe actin-related protein, Arp3, is a component of the cortical actin cytoskeleton and interacts with profilin.

McCollum D et al. EMBO J 1996 Dec 02;15(23):6438-46

PMID:20517925 - GMF is an evolutionarily developed Adf/cofilin-super family protein involved in the Arp2/3 complex-mediated organization of the actin cytoskeleton.

Nakano K et al. Cytoskeleton (Hoboken) 2010 Jun;67(6):373-82

PMID:10388810 - Identification and characterization of Schizosaccharomyces pombe asp1(+), a gene that interacts with mutations in the Arp2/3 complex and actin.

Feoktistova A et al. Genetics 1999 Jul;152(3):895-908

GO_REF:0000111 - Gene Ontology annotations Inferred by Curator (IC) using at least one Inferred by Sequence Similarity (ISS) annotation to support the inference

PMID:18165685 - Pathway of actin filament branch formation by Arp2/3 complex.

Beltzner CC et al. J Biol Chem 2008 Mar 14;283(11):7135-44

GO_REF:0000033 - Annotation inferences using phylogenetic trees

PMID:21676862 - Structural and biochemical characterization of two binding sites for nucleation-promoting factor WASp-VCA on Arp2/3 complex.

Ti SC et al. Proc Natl Acad Sci U S A 2011 Aug 16;108(33):E463-71

PMID:23148219 - Insertions within the actin core of actin-related protein 3 (Arp3) modulate branching nucleation by Arp2/3 complex.

Liu SL et al. J Biol Chem 2013 Jan 04;288(1):487-97

PMID:38448439 - Cryo-EM structures reveal how phosphate release from Arp3 weakens actin filament branches formed by Arp2/3 complex.

Chavali SS et al. Nat Commun 2024 Mar 06;15(1):2059

PMID:41850284 - Arp2/3-dependent actin assembly shapes endosomes and promotes intracellular trafficking in fission yeast.

Melero A et al. Curr Biol 2026 Mar 17;

PMID:37531259 - Membrane binding of endocytic myosin-1s is inhibited by a class of ankyrin repeat proteins.

Willet AH et al. Mol Biol Cell 2023 Oct 01;34(11):br17

PMID:21642440 - Actin filament bundling by fimbrin is important for endocytosis, cytokinesis, and polarization in fission yeast.

Skau CT et al. J Biol Chem 2011 Jul 29;286(30):26964-77

PMID:16823372 - ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe.

Matsuyama A et al. Nat Biotechnol 2006 Jul;24(7):841-7

PMID:30709916 - A Cdc42 GEF, Gef1, through endocytosis organizes F-BAR Cdc15 along the actomyosin ring and promotes concentric furrowing.

Onwubiko UN et al. J Cell Sci 2019 Feb 28;132(5)

PMID:16087707 - Interactions of WASp, myosin-I, and verprolin with Arp2/3 complex during actin patch assembly in fission yeast.

Sirotkin V et al. J Cell Biol 2005 Aug 15;170(4):637-48

PMID:30726745 - Fission Yeast NDR/LATS Kinase Orb6 Regulates Exocytosis via Phosphorylation of the Exocyst Complex.

Tay YD et al. Cell Rep 2019 Feb 05;26(6):1654-1667.e7

PMID:37970674 - SUMOylation regulates Lem2 function in centromere clustering and silencing.

Strachan J et al. J Cell Sci 2023 Dec 01;136(23)

PMID:10588653 - A mutant of Arp2p causes partial disassembly of the Arp2/3 complex and loss of cortical actin function in fission yeast.

Morrell JL et al. Mol Biol Cell 1999 Dec;10(12):4201-15

PMID:21511999 - Comparative functional genomics of the fission yeasts.

Rhind N et al. Science 2011 May 20;332(6032):930-6

PMID:20668161 - Proteasome nuclear import mediated by Arc3 can influence efficient DNA damage repair and mitosis in Schizosaccharomyces pombe.

Cabrera R et al. Mol Biol Cell 2010 Sep 15;21(18):3125-36

PMID:36442092 - Mechanism of actin filament branch formation by Arp2/3 complex revealed by a high-resolution cryo-EM structureof the branch junction.

Chou SZ et al. Proc Natl Acad Sci U S A 2022 Dec 06;119(49):e2206722119

PMID:16224022 - Counting cytokinesis proteins globally and locally in fission yeast.

Wu JQ et al. Science 2005 Oct 14;310(5746):310-4

PMID:16537923 - Sterol regulatory element binding protein is a principal regulator of anaerobic gene expression in fission yeast.

Todd BL et al. Mol Cell Biol 2006 Apr;26(7):2817-31

PMID:25543282 - Profilin regulates F-actin network homeostasis by favoring formin over Arp2/3 complex.

Suarez C et al. Dev Cell 2015 Jan 12;32(1):43-53

PMID:24763107 - Absolute proteome and phosphoproteome dynamics during the cell cycle of Schizosaccharomyces pombe (Fission Yeast).

Carpy A et al. Mol Cell Proteomics 2014 Aug;13(8):1925-36

PMID:15743909 - Profilin-mediated competition between capping protein and formin Cdc12p during cytokinesis in fission yeast.

Kovar DR et al. Mol Biol Cell 2005 May;16(5):2313-24

PMID:12214236 - Actin dynamics in the contractile ring during cytokinesis in fission yeast.

Pelham RJ et al. Nature 2002 Sep 05;419(6902):82-6

PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.

Hayles J et al. Open Biol 2013 May 22;3(5):130053

PMID:27417392 - Identification of an ATP-controlled allosteric switch that controls actin filament nucleation by Arp2/3 complex.

Rodnick-Smith M et al. Nat Commun 2016 Jul 15;7:12226

PMID:28218250 - Chromatin remodeller Fun30 Fft3 induces nucleosome disassembly to facilitate RNA polymerase II elongation.

Lee J et al. Nat Commun 2017 Feb 20;8:14527

PMID:30150414 - Conformational changes in Arp2/3 complex induced by ATP, WASp-VCA, and actin filaments.

Espinoza-Sanchez S et al. Proc Natl Acad Sci U S A 2018 Sep 11;115(37):E8642-E8651

PMID:19362000 - Cofilin dissociates Arp2/3 complex and branches from actin filaments.

Chan C et al. Curr Biol 2009 Apr 14;19(7):537-45

PMID:16864655 - Assembly of the cytokinetic contractile ring from a broad band of nodes in fission yeast.

Wu JQ et al. J Cell Biol 2006 Jul 31;174(3):391-402

PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.

Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623

PMID:39012625 - Arp2/3-dependent endocytosis ensures Cdc42 oscillations by removing Pak1-mediated negative feedback.

Harrell MA et al. J Cell Biol 2024 Sep 02;223(10)

PMID:40015273 - A comprehensive Schizosaccharomyces pombe atlas of physical transcription factor interactions with proteins and chromatin.

Skribbe M et al. Mol Cell 2025 Feb 19;

PMID:30471998 - Dip1 Co-opts Features of Branching Nucleation to Create Linear Actin Filaments that Activate WASP-Bound Arp2/3 Complex.

Balzer CJ et al. Curr Biol 2018 Dec 03;28(23):3886-3891.e4

PMID:9765059 - Subcellular localization and possible function of actin, tropomyosin and actin-related protein 3 (Arp3) in the fission yeast Schizosaccharomyces pombe.

Arai R et al. Eur J Cell Biol 1998 Aug;76(4):288-95

PMID:20624911 - Asp1, a conserved 1/3 inositol polyphosphate kinase, regulates the dimorphic switch in Schizosaccharomyces pombe.

Pöhlmann J et al. Mol Cell Biol 2010 Sep;30(18):4535-47

PMID:24127216 - The formins Cdc12 and For3 cooperate during contractile ring assembly in cytokinesis.

Coffman VC et al. J Cell Biol 2013 Oct 14;203(1):101-14

PMID:39367033 - Quantitative proteomics and phosphoproteomics profiling of meiotic divisions in the fission yeast Schizosaccharomyces pombe.

Sivakova B et al. Sci Rep 2024 Oct 04;14(1):23105

PMID:24120641 - Dip1 defines a class of Arp2/3 complex activators that function without preformed actin filaments.

Wagner AR et al. Curr Biol 2013 Oct 21;23(20):1990-8

PMID:8978670 - Fission yeast Sop2p: a novel and evolutionarily conserved protein that interacts with Arp3p and modulates profilin function.

Balasubramanian MK et al. EMBO J 1996 Dec 02;15(23):6426-37

PMID:1729722 - Identification of act2, an essential gene in the fission yeast Schizosaccharomyces pombe that encodes a protein related to actin.

Lees-Miller JP et al. Proc Natl Acad Sci U S A 1992 Jan 01;89(1):80-3

PMID:11294907 - Roles of a fimbrin and an alpha-actinin-like protein in fission yeast cell polarization and cytokinesis.

Wu JQ et al. Mol Biol Cell 2001 Apr;12(4):1061-77

PMID:21449051 - Schizosaccharomyces pombe Arc3 is a conserved subunit of the Arp2/3 complex required for polarity, actin organization, and endocytosis.

Cabrera R et al. Yeast 2011 Jun;28(6):495-503

PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.

Marguerat S et al. Cell 2012 Oct 26;151(3):671-83

PMID:32839613 - Cryo-EM reveals the transition of Arp2/3 complex from inactive to nucleation-competent state.

Shaaban M et al. Nat Struct Mol Biol 2020 Nov;27(11):1009-1016

PMID:31064814 - Proximity-dependent biotinylation mediated by TurboID to identify protein-protein interaction networks in yeast.

Larochelle M et al. J Cell Sci 2019 May 31;132(11)

PMID:25720772 - Quantitative phosphoproteomics reveals pathways for coordination of cell growth and division by the conserved fission yeast kinase pom1.

Kettenbach AN et al. Mol Cell Proteomics 2015 May;14(5):1275-87

PMID:16866873 - Actin-capping protein is involved in controlling organization of actin cytoskeleton together with ADF/cofilin, profilin and F-actin crosslinking proteins in fission yeast.

Nakano K et al. Genes Cells 2006 Aug;11(8):893-905

PMID:20587778 - Quantitative analysis of the mechanism of endocytic actin patch assembly and disassembly in fission yeast.

Sirotkin V et al. Mol Biol Cell 2010 Aug 15;21(16):2894-904

PMID:22730331 - Dual recruitment of Cdc48 (p97)-Ufd1-Npl4 ubiquitin-selective segregase by small ubiquitin-like modifier protein (SUMO) and ubiquitin in SUMO-targeted ubiquitin ligase-mediated genome stability functions.

Nie M et al. J Biol Chem 2012 Aug 24;287(35):29610-9