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protein coding gene - nbp35 (SPAC637.08) - CIA machinery ATPase Nbp35

Gene summary

Standard name
nbp35
Systematic ID
SPAC637.08
Product
CIA machinery ATPase Nbp35
Organism
Schizosaccharomyces pombe (fission yeast)
UniProt ID
O94442
ORFeome ID
13/13D10
Characterisation status
biological role inferred
Feature type
mRNA gene
Genomic location
chromosome I: 4553907..4555164 forward strand

Annotation

GO biological process

GO:0044572 - [4Fe-4S] cluster assembly

References:

GO cellular component

GO:0005829 - cytosol

References:

GO:1904564 - cytosolic [4Fe-4S] assembly scaffold complex

References:

GO:0005634 - nucleus

References:

GO molecular function

GO:0051539 - 4 iron, 4 sulfur cluster binding

References:

GO:0005524 - ATP binding

References:

GO:0016887 - ATP hydrolysis activity

References:

GO:0140663 - ATP-dependent FeS chaperone activity

References:

Modification

MOD:01148 - ubiquitinylated lysine

References:

Protein features

PBO:0111792 - AAA family ATPase

Quantitative gene expression

PBO:0006310 - protein level

References:

PBO:0011963 - RNA level

References:

Single locus phenotype

FYPO:0000311 - inviable after spore germination with normal, unseptated germ tube morphology

References:

Genotypes:

FYPO:0002061 - inviable vegetative cell population

References:

Genotypes:

Taxonomic conservation

PBO:0011067 - conserved in bacteria

PBO:0011065 - conserved in eukaryotes

PBO:0011064 - conserved in fungi

PBO:0011069 - conserved in metazoa

PBO:0011070 - conserved in vertebrates

PBO:0006222 - predominantly single copy (one to one)

Protein features

IDNameInterPro nameDB name
PF10609ParAYlxH/NBP35Pfam
cd02037Mrp_NBP35Mrp/NBP35_ATP-bdCDD
PS01215MRPMrp-like_CSPROSITE patterns
G3DSA:3.40.50.300:FF:000427CATH-FunFam
G3DSA:3.40.50.300P-loop_NTPaseCATH-Gene3D
SSF52540P-loop_NTPaseSUPERFAMILY
PTHR23264Mrp/NBP35_ATP-bdPANTHER
MF_02040Mrp_NBP35Mrp/NBP35_ATP-bdHAMAP
MF_03038NUBP1NUBP1/Nbp35HAMAP
mobidb-lite-Disorderdisorder_predictionMobiDB-Disorder

Orthologs

References / Literature

PMID:21511999 - Comparative functional genomics of the fission yeasts.
Rhind N et al. Science 2011 May 20;332(6032):930-6
GO_REF:0000002 - Comments
PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.
Hayles J et al. Open Biol 2013 May 22;3(5):130053
PMID:16823372 - ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe.
Matsuyama A et al. Nat Biotechnol 2006 Jul;24(7):841-7
PMID:24763107 - Absolute proteome and phosphoproteome dynamics during the cell cycle of Schizosaccharomyces pombe (Fission Yeast).
Carpy A et al. Mol Cell Proteomics 2014 Aug;13(8):1925-36
PMID:26099175 - The role of mitochondria and the CIA machinery in the maturation of cytosolic and nuclear iron-sulfur proteins.
Lill R et al. Eur J Cell Biol 2015;94(7-9):280-91
GO_REF:0000024 - Manual transfer of experimentally-verified manual GO annotation data to orthologs by curator judgment of sequence similarity.
PMID:40015273 - A comprehensive Schizosaccharomyces pombe atlas of physical transcription factor interactions with proteins and chromatin.
Skribbe M et al. Mol Cell 2025 Feb 19;
PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.
Marguerat S et al. Cell 2012 Oct 26;151(3):671-83
PMID:28218250 - Chromatin remodeller Fun30 Fft3 induces nucleosome disassembly to facilitate RNA polymerase II elongation.
Lee J et al. Nat Commun 2017 Feb 20;8:14527
PMID:34660592 - Biogenesis of Iron-Sulfur Clusters and Their Role in DNA Metabolism.
Shi R et al. Front Cell Dev Biol 2021;9:735678
PMID:37970674 - SUMOylation regulates Lem2 function in centromere clustering and silencing.
Strachan J et al. J Cell Sci 2023 Dec 01;136(23)
PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.
Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623