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protein coding gene - klp2 (SPAC664.10) - kinesin-14 family minus-end directed microtubule motor Klp2

Gene summary

Standard name
klp2
Systematic ID
SPAC664.10
Product
kinesin-14 family minus-end directed microtubule motor Klp2
Organism
Schizosaccharomyces pombe (fission yeast)
UniProt ID
Q9US03
ORFeome ID
29/29A08
Characterisation status
biological role published
Feature type
mRNA gene
Genomic location
chromosome I: 1721954..1724902 forward strand

Annotation

GO biological process

GO:0031122 - cytoplasmic microtubule organization

References:

GO:0030951 - establishment or maintenance of microtubule cytoskeleton polarity

References:

GO:0000742 - karyogamy involved in conjugation with cellular fusion

References:

GO:1990571 - meiotic centromere clustering

References:

GO:0090306 - meiotic spindle assembly

References:

GO:0140642 - meiotic spindle formation (spindle phase two)

References:

GO:1990810 - microtubule anchoring at mitotic spindle pole body

References:

GO:0001578 - microtubule bundle formation

References:

GO:0051012 - microtubule sliding

References:

GO:0031534 - minus-end directed microtubule sliding

References:

GO:1990942 - mitotic metaphase chromosome recapture

References:

GO:0000022 - mitotic spindle elongation

References:

GO:0140641 - mitotic spindle formation (spindle phase two)

References:

GO:0051256 - mitotic spindle midzone assembly

References:

GO:0007052 - mitotic spindle organization

References:

GO:0090561 - nuclear migration during mitotic telophase

References:

GO:0000743 - nuclear migration involved in conjugation with cellular fusion

References:

GO:0031535 - plus-end directed microtubule sliding

References:

GO cellular component

GO:0000235 - astral microtubule

References:

GO:0055028 - cortical microtubule

References:

GO:0005881 - cytoplasmic microtubule

References:

GO:0000776 - kinetochore

References:

GO:0090619 - meiotic spindle pole

References:

GO:0015630 - microtubule cytoskeleton

References:

GO:0036449 - microtubule minus-end

References:

GO:0005815 - microtubule organizing center

References:

GO:0035371 - microtubule plus-end

References:

GO:0005872 - minus-end kinesin complex

References:

GO:0072686 - mitotic spindle

References:

GO:1990537 - mitotic spindle polar microtubule

References:

GO:0005634 - nucleus

References:

GO:0005827 - polar microtubule

References:

GO:0097431 - mitotic spindle pole

References:

GO molecular function

GO:0005524 - ATP binding

References:

GO:0016887 - ATP hydrolysis activity

References:

GO:0008017 - microtubule binding

References:

GO:0008569 - minus-end-directed microtubule motor activity

References:

GO:0005515 - protein binding

References:

Modification

MOD:00046 - O-phospho-L-serine

References:

MOD:00047 - O-phospho-L-threonine

References:

MOD:00048 - O4'-phospho-L-tyrosine

References:

MOD:00696 - phosphorylated residue

References:

Multi-locus phenotype

FYPO:0007756 - abnormal homologous chromosome segregation with collapsed spindle

References:

Genotypes:

FYPO:0007080 - abnormal initial meiotic spindle pole body separation in meiosis I

References:

Genotypes:

FYPO:0001733 - abnormal mitotic spindle pole body separation

References:

Genotypes:

FYPO:0001894 - abnormal sporulation resulting in formation of ascus with more or fewer than four spores

References:

Genotypes:

FYPO:0007989 - abolished nuclear congression during mating

References:

Genotypes:

FYPO:0007150 - decreased microtubule bundle formation

References:

Genotypes:

FYPO:0005364 - decreased rate of kinetochore sliding during chromosome recapture

References:

Genotypes:

FYPO:0006041 - decreased rate of mitotic metaphase chromosome recapture

References:

Genotypes:

FYPO:0000513 - delayed onset of nuclear fusion during mating

References:

Genotypes:

FYPO:0004950 - elongated vegetative cell with elongated oscillating nucleus

References:

Genotypes:

FYPO:0005411 - increased number of unattached kinetochores

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Genotypes:

FYPO:0001489 - inviable vegetative cell

References:

Genotypes:

FYPO:0003787 - long mitotic spindle microtubules protruding beyond spindle pole body

References:

Genotypes:

FYPO:0000324 - mitotic metaphase/anaphase transition delay

References:

Genotypes:

FYPO:0000276 - monopolar mitotic spindle

References:

Genotypes:

FYPO:0005720 - normal mitotic metaphase chromosome recapture

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Genotypes:

FYPO:0007099 - normal spindle elongation in meiosis I

References:

Genotypes:

FYPO:0000590 - normal sporulation

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Genotypes:

FYPO:0004395 - short bipolar mitotic spindle during metaphase

References:

Genotypes:

FYPO:0007755 - short meiotic spindle during metaphase I

References:

Genotypes:

FYPO:0007744 - spindle collapse during meiotic prophase I

References:

Genotypes:

FYPO:0006884 - thick mitotic spindle during mitotic metaphase

References:

Genotypes:

FYPO:0002058 - viable cell population

References:

Genotypes:

FYPO:0002060 - viable vegetative cell population

References:

Genotypes:

Protein features

PBO:0111839 - kinesin Kar3 subfamily

Quantitative gene expression

PBO:0006310 - protein level

References:

PBO:0011963 - RNA level

References:

Single locus phenotype

FYPO:0007756 - abnormal homologous chromosome segregation with collapsed spindle

References:

Genotypes:

FYPO:0007108 - abnormal meiotic centromere clustering during meiotic prophase I

References:

Genotypes:

FYPO:0000151 - abnormal meiotic chromosome segregation

References:

Genotypes:

FYPO:0006343 - abolished microtubule bundle formation

References:

Genotypes:

FYPO:0000705 - abolished protein-protein interaction

References:

Genotypes:

FYPO:0005363 - decreased kinetochore microtubule depolymerization during mitotic metaphase chromosome recapture

References:

Genotypes:

FYPO:0006342 - decreased microtubule binding

References:

Genotypes:

FYPO:0007150 - decreased microtubule bundle formation

References:

Genotypes:

FYPO:0007749 - decreased protein localization to meiotic spindle during meiosis I

References:

Genotypes:

FYPO:0000933 - decreased protein localization to microtubule cytoskeleton during vegetative growth

References:

Genotypes:

FYPO:0001838 - decreased protein phosphorylation during vegetative growth

References:

Genotypes:

FYPO:0003268 - decreased rate of mitotic spindle elongation

References:

Genotypes:

FYPO:0000513 - delayed onset of nuclear fusion during mating

References:

Genotypes:

FYPO:0007748 - increased duration of spindle assembly during meiosis I

References:

Genotypes:

FYPO:0002061 - inviable vegetative cell population

References:

Genotypes:

FYPO:0000733 - long mitotic spindle

References:

Genotypes:

FYPO:0004307 - long mitotic spindle during metaphase

References:

Genotypes:

FYPO:0000276 - monopolar mitotic spindle

References:

Genotypes:

FYPO:0007747 - normal meiotic spindle length during prophase I

References:

Genotypes:

FYPO:0001513 - normal mitotic sister chromatid segregation

References:

Genotypes:

FYPO:0006259 - normal mitotic spindle length during metaphase

References:

Genotypes:

FYPO:0007750 - normal protein localization to meiotic spindle during meiosis I

References:

Genotypes:

FYPO:0004692 - normal protein localization to mitotic spindle midzone

References:

Genotypes:

FYPO:0002967 - normal protein localization to mitotic spindle pole body

References:

Genotypes:

FYPO:0005342 - normal rate of mitotic spindle elongation during anaphase B

References:

Genotypes:

FYPO:0003187 - protein mislocalized to astral microtubule during mitosis

References:

Genotypes:

FYPO:0002693 - resistance to diamide

References:

Genotypes:

FYPO:0003383 - resistance to tert-butyl hydroperoxide

References:

Genotypes:

FYPO:0001719 - sensitive to lithium

References:

Genotypes:

FYPO:0003305 - separate mitotic half spindles present

References:

Genotypes:

FYPO:0007751 - spindle collapse during meiotic prometaphase I

References:

Genotypes:

FYPO:0001491 - viable vegetative cell

References:

Genotypes:

FYPO:0002060 - viable vegetative cell population

References:

Genotypes:

FYPO:0002177 - viable vegetative cell with normal cell morphology

References:

Genotypes:

Taxonomic conservation

PBO:0011065 - conserved in eukaryotes

PBO:0011071 - conserved in eukaryotes only

PBO:0011064 - conserved in fungi

PBO:0011069 - conserved in metazoa

PBO:0011070 - conserved in vertebrates

Protein features

IDNameInterPro nameDB name
PF00225KinesinKinesin_motor_domPFAM
cd01366KISc_C_terminalCDD
PS00411KINESIN_MOTOR_1Kinesin_motor_CSPROSITE_PATTERNS
PS50067KINESIN_MOTOR_2Kinesin_motor_domPROSITE_PROFILES
SM00129kinesin_4Kinesin_motor_domSMART
PR00380KINESINHEAVYKinesin_motor_domPRINTS
G3DSA:3.40.850.10:FF:000065FUNFAM
SSF52540P-loop containing nucleoside triphosphate hydrolasesP-loop_NTPaseSUPERFAMILY
G3DSA:3.40.850.10Kinesin motor domainKinesin_motor_dom_sfGENE3D
PTHR47972KINESIN-LIKE PROTEIN KLP-3Kinesin-like_famPANTHER
CoilCoilCOILS
mobidb-lite-Disorderdisorder_predictionMOBIDB-Disorder
mobidb-lite-Low-complexitydisorder_predictionMOBIDB-Low-complexity
mobidb-lite-Polardisorder_predictionMOBIDB-Polar

Orthologs

References / Literature

PMID:17409356 - Mitotic chromosome biorientation in fission yeast is enhanced by dynein and a minus-end-directed, kinesin-like protein.
Grishchuk EL et al. Mol Biol Cell 2007 Jun;18(6):2216-25
GO_REF:0000033 - Annotation inferences using phylogenetic trees
PMID:29996109 - Quantitative Phosphoproteomics Reveals the Signaling Dynamics of Cell-Cycle Kinases in the Fission Yeast Schizosaccharomyces pombe.
Swaffer MP et al. Cell Rep 2018 Jul 10;24(2):503-514
PMID:24039245 - The human kinesin-14 HSET tracks the tips of growing microtubules in vitro.
Braun M et al. Cytoskeleton (Hoboken) 2013 Sep;70(9):515-21
PMID:27834216 - Schizosaccharomyces pombe kinesin-5 switches direction using a steric blocking mechanism.
Britto M et al. Proc Natl Acad Sci U S A 2016 Nov 22;113(47):E7483-E7489
PMID:25720772 - Quantitative phosphoproteomics reveals pathways for coordination of cell growth and division by the conserved fission yeast kinase pom1.
Kettenbach AN et al. Mol Cell Proteomics 2015 May;14(5):1275-87
PMID:18256284 - Sister kinetochore recapture in fission yeast occurs by two distinct mechanisms, both requiring Dam1 and Klp2.
Gachet Y et al. Mol Biol Cell 2008 Apr;19(4):1646-62
PMID:17254972 - Crosslinkers and motors organize dynamic microtubules to form stable bipolar arrays in fission yeast.
Janson ME et al. Cell 2007 Jan 26;128(2):357-68
PMID:19430466 - The kinesin-14 Klp2 organizes microtubules into parallel bundles by an ATP-dependent sorting mechanism.
Braun M et al. Nat Cell Biol 2009 Jun;11(6):724-30
PMID:16823372 - ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe.
Matsuyama A et al. Nat Biotechnol 2006 Jul;24(7):841-7
GO_REF:0000002 - Comments
PMID:17036054 - Microtubule depolymerization can drive poleward chromosome motion in fission yeast.
Grishchuk EL et al. EMBO J 2006 Oct 18;25(20):4888-96
PMID:23087209 - The kinesin-14 Klp2 is negatively regulated by the SIN for proper spindle elongation and telophase nuclear positioning.
Mana-Capelli S et al. Mol Biol Cell 2012 Dec;23(23):4592-600
PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.
Marguerat S et al. Cell 2012 Oct 26;151(3):671-83
PMID:25869666 - Microtubule minus end motors kinesin-14 and dynein drive nuclear congression in parallel pathways.
Scheffler K et al. J Cell Biol 2015 Apr 13;209(1):47-58
PMID:26124291 - A stable microtubule array drives fission yeast polarity reestablishment upon quiescence exit.
Laporte D et al. J Cell Biol 2015 Jul 06;210(1):99-113
PMID:27298342 - Identification of S-phase DNA damage-response targets in fission yeast reveals conservation of damage-response networks.
Willis NA et al. Proc Natl Acad Sci U S A 2016 Jun 28;113(26):E3676-85
PMID:22681890 - Hierarchical modularity and the evolution of genetic interactomes across species.
Ryan CJ et al. Mol Cell 2012 Jun 08;46(5):691-704
PMID:28513584 - Kinesin-5-independent mitotic spindle assembly requires the antiparallel microtubule crosslinker Ase1 in fission yeast.
Rincon SA et al. Nat Commun 2017 May 17;8:15286
PMID:30463883 - Suppressor Analysis Uncovers That MAPs and Microtubule Dynamics Balance with the Cut7/Kinesin-5 Motor for Mitotic Spindle Assembly in Schizosaccharomyces pombe .
Yukawa M et al. G3 (Bethesda) 2019 Jan 09;9(1):269-280
PMID:24497846 - A chaperone-assisted degradation pathway targets kinetochore proteins to ensure genome stability.
Kriegenburg F et al. PLoS Genet 2014 Jan;10(1):e1004140
PMID:16002618 - The kinesin Klp2 mediates polarization of interphase microtubules in fission yeast.
Carazo-Salas RE et al. Science 2005 Jul 08;309(5732):297-300
PMID:29684553 - Fission yeast cells overproducing HSET/KIFC1 provides a useful tool for identification and evaluation of human kinesin-14 inhibitors.
Yukawa M et al. Fungal Genet Biol 2018 Jul;116:33-41
PMID:21151990 - The fission yeast XMAP215 homolog Dis1p is involved in microtubule bundle organization.
Roque H et al. PLoS One 2010 Dec 02;5(12):e14201
PMID:24239120 - Antagonistic spindle motors and MAPs regulate metaphase spindle length and chromosome segregation.
Syrovatkina V et al. Curr Biol 2013 Dec 02;23(23):2423-9
PMID:29167352 - Two spatially distinct kinesin-14 proteins, Pkl1 and Klp2, generate collaborative inward forces against kinesin-5 Cut7 in S. pombe .
Yukawa M et al. J Cell Sci 2018 Jan 04;131(1)
PMID:30726745 - Fission Yeast NDR/LATS Kinase Orb6 Regulates Exocytosis via Phosphorylation of the Exocyst Complex.
Tay YD et al. Cell Rep 2019 Feb 05;26(6):1654-1667.e7
PMID:32062975 - Phosphoproteomics Reveals Novel Targets and Phosphoprotein Networks in Cell Cycle Mediated by Dsk1 Kinase.
Wu M et al. J Proteome Res 2020 Apr 03;19(4):1776-1787
PMID:32723864 - Klp2 and Ase1 synergize to maintain meiotic spindle stability during metaphase I.
Zheng F et al. J Biol Chem 2020 Sep 18;295(38):13287-13298
PMID:11694582 - pkl1(+)and klp2(+): Two kinesins of the Kar3 subfamily in fission yeast perform different functions in both mitosis and meiosis.
Troxell CL et al. Mol Biol Cell 2001 Nov;12(11):3476-88
PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.
Hayles J et al. Open Biol 2013 May 22;3(5):130053
PMID:37787768 - Broad functional profiling of fission yeast proteins using phenomics and machine learning.
Rodríguez-López M et al. Elife 2023 Oct 03;12
PMID:24763107 - Absolute proteome and phosphoproteome dynamics during the cell cycle of Schizosaccharomyces pombe (Fission Yeast).
Carpy A et al. Mol Cell Proteomics 2014 Aug;13(8):1925-36
PMID:30806623 - Kinesin-6 regulates cell-size-dependent spindle elongation velocity to keep mitosis duration constant in fission yeast.
Krüger LK et al. Elife 2019 Feb 26;8
PMID:32327557 - Kinesin-14 family proteins and microtubule dynamics define S. pombe mitotic and meiotic spindle assembly, and elongation.
Loncar A et al. J Cell Sci 2020 Jun 08;133(11)
PMID:11018050 - Tea2p is a kinesin-like protein required to generate polarized growth in fission yeast.
Browning H et al. J Cell Biol 2000 Oct 02;151(1):15-28
PMID:34951983 - Force by minus-end motors Dhc1 and Klp2 collapses the S. pombe spindle after laser ablation.
Zareiesfandabadi P et al. Biophys J 2022 Jan 18;121(2):263-276
PMID:23770679 - Microtubules and Alp7-Alp14 (TACC-TOG) reposition chromosomes before meiotic segregation.
Kakui Y et al. Nat Cell Biol 2013 Jul;15(7):786-96
PMID:22264609 - A role for metaphase spindle elongation forces in correction of merotelic kinetochore attachments.
Choi SH et al. Curr Biol 2012 Feb 07;22(3):225-30
PMID:25987607 - The Msd1-Wdr8-Pkl1 complex anchors microtubule minus ends to fission yeast spindle pole bodies.
Yukawa M et al. J Cell Biol 2015 May 25;209(4):549-62
PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.
Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623
PMID:19686686 - Phospho-regulated interaction between kinesin-6 Klp9p and microtubule bundler Ase1p promotes spindle elongation.
Fu C et al. Dev Cell 2009 Aug;17(2):257-67
PMID:33946513 - The Putative RNA-Binding Protein Dri1 Promotes the Loading of Kinesin-14/Klp2 to the Mitotic Spindle and Is Sequestered into Heat-Induced Protein Aggregates in Fission Yeast.
Yukawa M et al. Int J Mol Sci 2021 Apr 30;22(9)
PMID:29259000 - Genes Important for Schizosaccharomyces pombe Meiosis Identified Through a Functional Genomics Screen.
Blyth J et al. Genetics 2018 Feb;208(2):589-603
PMID:31532702 - Kinetochore-mediated outward force promotes spindle pole separation in fission yeast.
Shirasugi Y et al. Mol Biol Cell 2019 Oct 15;30(22):2802-2813
PMID:21511999 - Comparative functional genomics of the fission yeasts.
Rhind N et al. Science 2011 May 20;332(6032):930-6
PMID:16611238 - The carboxy-terminus of Alp4 alters microtubule dynamics to induce oscillatory nuclear movement led by the spindle pole body in Schizosaccharomyces pombe.
Masuda H et al. Genes Cells 2006 Apr;11(4):337-52