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protein coding gene - ssc1 (SPAC664.11) - PAM complex ATPase motor/mitochondrial [2Fe-2S] cluster assembly chaperone Ssc1

Gene summary

Standard name
ssc1
Systematic ID
SPAC664.11
Product
PAM complex ATPase motor/mitochondrial [2Fe-2S] cluster assembly chaperone Ssc1
Organism
Schizosaccharomyces pombe (fission yeast)
Synonyms
ssp1
UniProt ID
P22774
ORFeome ID
37/37B12
Characterisation status
biological role published
Feature type
mRNA gene
Genomic location
chromosome I: 1725752..1728189 forward strand

Annotation

Disease association

MONDO:0008422 - autosomal dominant sideroblastic anemia

References:

MONDO:0014801 - even-plus syndrome

References:

GO biological process

GO:0044571 - [2Fe-2S] cluster assembly

References:

GO:0030150 - protein import into mitochondrial matrix

References:

GO:0042026 - protein refolding

References:

GO cellular component

GO:0005739 - mitochondrion

References:

GO:0001405 - PAM complex, Tim23 associated import motor

References:

GO molecular function

GO:0005524 - ATP binding

References:

GO:0016887 - ATP hydrolysis activity

References:

GO:0140662 - ATP-dependent protein folding chaperone

References:

GO:0031072 - heat shock protein binding

References:

GO:0140388 - protein translocation chaperone activity

References:

Modification

MOD:00046 - O-phospho-L-serine

References:

MOD:00047 - O-phospho-L-threonine

References:

MOD:00696 - phosphorylated residue

References:

MOD:01148 - ubiquitinylated lysine

References:

Protein sequence feature

SO:0001808 - mitochondrial_targeting_signal

References:

Qualitative gene expression

PomGeneEx:0000019 - protein level decreased

References:

PomGeneEx:0000012 - RNA level decreased

References:

PomGeneEx:0000011 - RNA level increased

References:

PomGeneEx:0000014 - RNA present

References:

Quantitative gene expression

PBO:0006310 - protein level

References:

PBO:0011963 - RNA level

References:

Single locus phenotype

FYPO:0002151 - inviable spore

References:

Genotypes:

FYPO:0002061 - inviable vegetative cell population

References:

Genotypes:

Taxonomic conservation

PBO:0011067 - conserved in bacteria

PBO:0011065 - conserved in eukaryotes

PBO:0011064 - conserved in fungi

PBO:0011069 - conserved in metazoa

PBO:0011070 - conserved in vertebrates

PBO:0006222 - predominantly single copy (one to one)

Protein features

IDNameInterPro nameDB name
PF00012HSP70Hsp_70_famPFAM
cd11734ASKHA_NBD_HSP70_Ssc1_3CDD
PS00297HSP70_1Heat_shock_70_CSPROSITE_PATTERNS
PS00329HSP70_2Heat_shock_70_CSPROSITE_PATTERNS
PS01036HSP70_3Heat_shock_70_CSPROSITE_PATTERNS
PR00301HEATSHOCK70PRINTS
G3DSA:3.90.640.10:FF:000003FUNFAM
G3DSA:3.30.30.30:FF:000003FUNFAM
G3DSA:3.30.420.40:FF:000004FUNFAM
G3DSA:2.60.34.10:FF:000014FUNFAM
G3DSA:1.20.1270.10:FF:000007FUNFAM
SSF100934Heat shock protein 70kD (HSP70), C-terminal subdomainHSP70_C_sfSUPERFAMILY
SSF100920Heat shock protein 70kD (HSP70), peptide-binding domainHSP70_peptide-bd_sfSUPERFAMILY
SSF53067Actin-like ATPase domainATPase_NBDSUPERFAMILY
G3DSA:3.90.640.10Actin; Chain A, domain 4GENE3D
G3DSA:2.60.34.10Substrate Binding Domain Of DNAk; Chain A, domain 1HSP70_peptide-bd_sfGENE3D
G3DSA:3.30.420.40GENE3D
G3DSA:1.20.1270.10HSP70_C_sfGENE3D
PTHR19375HEAT SHOCK PROTEIN 70KDAHsp_70_famPANTHER
CoilCoilCOILS
MF_00332DnaKChaperone_DnaKHAMAP
mobidb-lite-Disorderdisorder_predictionMOBIDB-Disorder
mobidb-lite-Polardisorder_predictionMOBIDB-Polar
NF001413PRK00290.1NCBIFAM
TIGR02350prok_dnaKChaperone_DnaKNCBIFAM

Orthologs

References / Literature

PMID:22540037 - Predicting the fission yeast protein interaction network.
Pancaldi V et al. G3 (Bethesda) 2012 Apr;2(4):453-67
PMID:21340088 - Microarray-based target identification using drug hypersensitive fission yeast expressing ORFeome.
Arita Y et al. Mol Biosyst 2011 May;7(5):1463-72
PMID:21828039 - Mfc1 is a novel forespore membrane copper transporter in meiotic and sporulating cells.
Beaudoin J et al. J Biol Chem 2011 Sep 30;286(39):34356-72
PMID:39367033 - Quantitative proteomics and phosphoproteomics profiling of meiotic divisions in the fission yeast Schizosaccharomyces pombe.
Sivakova B et al. Sci Rep 2024 Oct 04;14(1):23105
PMID:20085751 - Monothiol glutaredoxin Grx5 interacts with Fe-S scaffold proteins Isa1 and Isa2 and supports Fe-S assembly and DNA integrity in mitochondria of fission yeast.
Kim KD et al. Biochem Biophys Res Commun 2010 Feb 12;392(3):467-72
PB_REF:0000006 - Disease associations from Monarch via human-pombe orthologs
PMID:38269097 - Rapamycin-sensitive mechanisms confine the growth of fission yeast below the temperatures detrimental to cell physiology.
Morozumi Y et al. iScience 2024 Jan 19;27(1):108777
PMID:19547744 - Evolution of phosphoregulation: comparison of phosphorylation patterns across yeast species.
Beltrao P et al. PLoS Biol 2009 Jun 16;7(6):e1000134
PMID:24497846 - A chaperone-assisted degradation pathway targets kinetochore proteins to ensure genome stability.
Kriegenburg F et al. PLoS Genet 2014 Jan;10(1):e1004140
PMID:2123810 - Isolation of a gene encoding a mitochondrial HSP70 protein from Schizosaccharomyces pombe.
Powell MJ et al. Gene 1990 Oct 30;95(1):105-10
PMID:36408920 - UniProt: the Universal Protein Knowledgebase in 2023.
UniProt Consortium Nucleic Acids Res 2023 Jan 06;51(D1):D523-D531
PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.
Marguerat S et al. Cell 2012 Oct 26;151(3):671-83
PMID:21511999 - Comparative functional genomics of the fission yeasts.
Rhind N et al. Science 2011 May 20;332(6032):930-6
PMID:29996109 - Quantitative Phosphoproteomics Reveals the Signaling Dynamics of Cell-Cycle Kinases in the Fission Yeast Schizosaccharomyces pombe.
Swaffer MP et al. Cell Rep 2018 Jul 10;24(2):503-514
PMID:40015273 - A comprehensive Schizosaccharomyces pombe atlas of physical transcription factor interactions with proteins and chromatin.
Skribbe M et al. Mol Cell 2025 Feb 19;
PMID:21652630 - Characterization of Mug33 reveals complementary roles for actin cable-dependent transport and exocyst regulators in fission yeast exocytosis.
Snaith HA et al. J Cell Sci 2011 Jul 01;124(Pt 13):2187-99
PMID:16823372 - ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe.
Matsuyama A et al. Nat Biotechnol 2006 Jul;24(7):841-7
PMID:27257059 - Ribosome recycling defects modify the balance between the synthesis and assembly of specific subunits of the oxidative phosphorylation complexes in yeast mitochondria.
Ostojić J et al. Nucleic Acids Res 2016 Jul 08;44(12):5785-97
GO_REF:0000024 - Manual transfer of experimentally-verified manual GO annotation data to orthologs by curator judgment of sequence similarity.
PMID:36361590 - Defining the Functional Interactome of Spliceosome-Associated G-Patch Protein Gpl1 in the Fission Yeast Schizosaccharomyces pombe .
Selicky T et al. Int J Mol Sci 2022 Oct 24;23(21)
PMID:21712547 - Mitotic substrates of the kinase aurora with roles in chromatin regulation identified through quantitative phosphoproteomics of fission yeast.
Koch A et al. Sci Signal 2011 Jun 28;4(179):rs6
PMID:1923816 - Dual modes of transcriptional and translational initiation of SSP1, the gene for a mitochondrial HSP70, responding to heat-shock in Schizosaccharomyces pombe.
Kasai H et al. Nucleic Acids Res 1991 Oct 11;19(19):5331-7
PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.
Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623
PMID:25720772 - Quantitative phosphoproteomics reveals pathways for coordination of cell growth and division by the conserved fission yeast kinase pom1.
Kettenbach AN et al. Mol Cell Proteomics 2015 May;14(5):1275-87
PMID:23297348 - Comprehensive proteomics analysis reveals new substrates and regulators of the fission yeast clp1/cdc14 phosphatase.
Chen JS et al. Mol Cell Proteomics 2013 May;12(5):1074-86
PMID:26545917 - Iron-Sulfur Cluster Biogenesis Chaperones: Evidence for Emergence of Mutational Robustness of a Highly Specific Protein-Protein Interaction.
Delewski W et al. Mol Biol Evol 2016 Mar;33(3):643-56
GO_REF:0000033 - Annotation inferences using phylogenetic trees
PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.
Hayles J et al. Open Biol 2013 May 22;3(5):130053
PMID:27984744 - Survival in Quiescence Requires the Euchromatic Deployment of Clr4/SUV39H by Argonaute-Associated Small RNAs.
Joh RI et al. Mol Cell 2016 Dec 15;64(6):1088-1101
PMID:30726745 - Fission Yeast NDR/LATS Kinase Orb6 Regulates Exocytosis via Phosphorylation of the Exocyst Complex.
Tay YD et al. Cell Rep 2019 Feb 05;26(6):1654-1667.e7
PMID:23956636 - Possible Roles of LAMMER Kinase Lkh1 in Fission Yeast by Comparative Proteome Analysis.
Cho SJ et al. Mycobiology 2010 Jun;38(2):108-12
PMID:33313903 - Ribosome profiling reveals ribosome stalling on tryptophan codons and ribosome queuing upon oxidative stress in fission yeast.
Rubio A et al. Nucleic Acids Res 2021 Jan 11;49(1):383-399
GO_REF:0000002 - Comments
PMID:18257517 - Phosphoproteome analysis of fission yeast.
Wilson-Grady JT et al. J Proteome Res 2008 Mar;7(3):1088-97
PMID:37970674 - SUMOylation regulates Lem2 function in centromere clustering and silencing.
Strachan J et al. J Cell Sci 2023 Dec 01;136(23)
PMID:39705284 - Proteomic and phosphoproteomic analyses reveal that TORC1 is reactivated by pheromone signaling during sexual reproduction in fission yeast.
Bérard M et al. PLoS Biol 2024 Dec 20;22(12):e3002963
PMID:24763107 - Absolute proteome and phosphoproteome dynamics during the cell cycle of Schizosaccharomyces pombe (Fission Yeast).
Carpy A et al. Mol Cell Proteomics 2014 Aug;13(8):1925-36