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protein coding gene - slx4 (SPAC688.06c) - structure-specific endonuclease subunit Slx4

Gene summary

Standard name
slx4
Systematic ID
SPAC688.06c
Product
structure-specific endonuclease subunit Slx4
Organism
Schizosaccharomyces pombe (fission yeast)
UniProt ID
Q9P6M0
ORFeome ID
20/20G07
Characterisation status
biological role published
Feature type
mRNA gene
Genomic location
chromosome I: 3116880..3119173 reverse strand

Annotation

Disease association

MONDO:0019391 - Fanconi anemia

References:

MONDO:0013499 - Fanconi anemia complementation group P

References:

GO biological process

GO:0000727 - double-strand break repair via break-induced replication

References:

GO:0043007 - maintenance of rDNA

References:

GO:0033260 - nuclear DNA replication

References:

GO:0031297 - replication fork processing

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GO cellular component

GO:0005634 - nucleus

References:

GO:0030875 - rDNA protrusion

References:

GO:0033557 - Slx1-Slx4 complex

References:

GO molecular function

GO:0005515 - protein binding

References:

Modification

MOD:00046 - O-phospho-L-serine

References:

Multi-locus phenotype

FYPO:0007140 - inviable after spore germination, multiple cell divisions, swollen, elongated cell

References:

Genotypes:

FYPO:0002061 - inviable vegetative cell population

References:

Genotypes:

FYPO:0000969 - normal growth during cellular response to UV

References:

Genotypes:

FYPO:0000963 - normal growth on hydroxyurea

References:

Genotypes:

FYPO:0001357 - normal vegetative cell population growth

References:

Genotypes:

FYPO:0005775 - sensitive to acetaldehyde

References:

Genotypes:

Protein features

PBO:0111804 - SAP domain

Qualitative gene expression

PomGeneEx:0000018 - protein level increased

References:

PomGeneEx:0000011 - RNA level increased

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Quantitative gene expression

PBO:0011963 - RNA level

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Single locus phenotype

FYPO:0002037 - decreased endodeoxyribonuclease activity

References:

Genotypes:

FYPO:0005777 - increased number of Rad52 foci during cellular response to acetaldehyde

References:

Genotypes:

FYPO:0006518 - loss of viability in G0

References:

Genotypes:

FYPO:0007553 - normal G1 to G0 transition

References:

Genotypes:

FYPO:0000969 - normal growth during cellular response to UV

References:

Genotypes:

FYPO:0000963 - normal growth on hydroxyurea

References:

Genotypes:

FYPO:0000478 - normal meiosis

References:

Genotypes:

FYPO:0001357 - normal vegetative cell population growth

References:

Genotypes:

FYPO:0009043 - resistance to potassium chloride and sodium dodecyl sulfate

References:

Genotypes:

FYPO:0003383 - resistance to tert-butyl hydroperoxide

References:

Genotypes:

FYPO:0000104 - sensitive to cycloheximide

References:

Genotypes:

FYPO:0007924 - sensitive to potassium chloride and sodium dodecyl sulfate

References:

Genotypes:

FYPO:0002060 - viable vegetative cell population

References:

Genotypes:

FYPO:0002177 - viable vegetative cell with normal cell morphology

References:

Genotypes:

Subunit composition

PBO:0015221 - heteromeric(2)

References:

Taxonomic conservation

PBO:0011065 - conserved in eukaryotes

PBO:0011071 - conserved in eukaryotes only

PBO:0011064 - conserved in fungi

PBO:0011069 - conserved in metazoa

PBO:0011070 - conserved in vertebrates

PBO:0006222 - predominantly single copy (one to one)

Protein features

IDNameInterPro nameDB name
PF09494Slx4Slx4_HTHPfam
CoilCOILS

Orthologs

References / Literature

PMID:14528010 - Slx1-Slx4 are subunits of a structure-specific endonuclease that maintains ribosomal DNA in fission yeast.
Coulon S et al. Mol Biol Cell 2004 Jan;15(1):71-80
PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.
Hayles J et al. Open Biol 2013 May 22;3(5):130053
PMID:16710300 - Sws1 is a conserved regulator of homologous recombination in eukaryotic cells.
Martín V et al. EMBO J 2006 Jun 07;25(11):2564-74
PMID:30726745 - Fission Yeast NDR/LATS Kinase Orb6 Regulates Exocytosis via Phosphorylation of the Exocyst Complex.
Tay YD et al. Cell Rep 2019 Feb 05;26(6):1654-1667.e7
PMID:12161753 - The transcriptional program of meiosis and sporulation in fission yeast.
Mata J et al. Nat Genet 2002 Sep;32(1):143-7
PMID:27687866 - Genetic controls of DNA damage avoidance in response to acetaldehyde in fission yeast.
Noguchi C et al. Cell Cycle 2017 Jan 02;16(1):45-58
PMID:27984744 - Survival in Quiescence Requires the Euchromatic Deployment of Clr4/SUV39H by Argonaute-Associated Small RNAs.
Joh RI et al. Mol Cell 2016 Dec 15;64(6):1088-1101
PMID:16467377 - Rad22Rad52-dependent repair of ribosomal DNA repeats cleaved by Slx1-Slx4 endonuclease.
Coulon S et al. Mol Biol Cell 2006 Apr;17(4):2081-90
PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.
Marguerat S et al. Cell 2012 Oct 26;151(3):671-83
PMID:21511999 - Comparative functional genomics of the fission yeasts.
Rhind N et al. Science 2011 May 20;332(6032):930-6
PB_REF:0000006 - Disease associations from Monarch via human-pombe orthologs
PMID:32269268 - Abo1 is required for the H3K9me2 to H3K9me3 transition in heterochromatin.
Dong W et al. Sci Rep 2020 Apr 08;10(1):6055
GO_REF:0000024 - Manual transfer of experimentally-verified manual GO annotation data to orthologs by curator judgment of sequence similarity.
PB_REF:0000003 - Disease Association Curation
PMID:26787556 - Crystal structure and SUMO binding of Slx1-Slx4 complex.
Lian FM et al. Sci Rep 2016 Jan 20;6:19331
PMID:22681890 - Hierarchical modularity and the evolution of genetic interactomes across species.
Ryan CJ et al. Mol Cell 2012 Jun 08;46(5):691-704
PMID:16169489 - Novel genes required for meiotic chromosome segregation are identified by a high-throughput knockout screen in fission yeast.
Gregan J et al. Curr Biol 2005 Sep 20;15(18):1663-9
PMID:16823372 - ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe.
Matsuyama A et al. Nat Biotechnol 2006 Jul;24(7):841-7
PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.
Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623
PMID:33260998 - High-Throughput Flow Cytometry Combined with Genetic Analysis Brings New Insights into the Understanding of Chromatin Regulation of Cellular Quiescence.
Zahedi Y et al. Int J Mol Sci 2020 Nov 27;21(23)
PMID:32282918 - Genetic interactions and transcriptomics implicate fission yeast CTD prolyl isomerase Pin1 as an agent of RNA 3' processing and transcription termination that functions via its effects on CTD phosphatase Ssu72.
Sanchez AM et al. Nucleic Acids Res 2020 May 21;48(9):4811-4826
PMID:37787768 - Broad functional profiling of fission yeast proteins using phenomics and machine learning.
Rodríguez-López M et al. Elife 2023 Oct 03;12
PMID:39367033 - Quantitative proteomics and phosphoproteomics profiling of meiotic divisions in the fission yeast Schizosaccharomyces pombe.
Sivakova B et al. Sci Rep 2024 Oct 04;14(1):23105