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protein coding gene - pqr1 (SPAC6B12.07c) - ubiquitin-protein ligase E3, phosphate quantity regulator Pqr1/Spx1

Gene summary

Standard name
pqr1
Systematic ID
SPAC6B12.07c
Product
ubiquitin-protein ligase E3, phosphate quantity regulator Pqr1/Spx1
Organism
Schizosaccharomyces pombe (fission yeast)
Synonyms
spx1
UniProt ID
O14212
ORFeome ID
21/21C02
Characterisation status
biological role published
Feature type
mRNA gene
Genomic location
chromosome I: 2421446..2423826 reverse strand

Annotation

GO biological process

GO:2000186 - negative regulation of phosphate transmembrane transport

References:

GO cellular component

GO:0005829 - cytosol

References:

GO molecular function

GO:0046872 - metal ion binding

References:

GO:0061630 - ubiquitin protein ligase activity

References:

Modification

MOD:01148 - ubiquitinylated lysine

References:

Multi-locus phenotype

FYPO:0008228 - abnormal phosphate export

References:

Genotypes:

FYPO:0000364 - abnormal plasma membrane morphology during vegetative growth

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Genotypes:

FYPO:0001045 - decreased acid phosphatase activity

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Genotypes:

FYPO:0000082 - decreased cell population growth at high temperature

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Genotypes:

FYPO:0000080 - decreased cell population growth at low temperature

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Genotypes:

FYPO:0008024 - decreased cellular polyphosphate level

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Genotypes:

FYPO:0008269 - decreased total cellular phosphate (Pi) level

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Genotypes:

FYPO:0001355 - decreased vegetative cell population growth

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Genotypes:

FYPO:0002243 - increased acid phosphatase activity

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Genotypes:

FYPO:0007816 - increased cellular polyphosphate level

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Genotypes:

FYPO:0008227 - increased free cellular phosphate (Pi) level

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Genotypes:

FYPO:0008027 - increased rate of acid phosphatase activation during phosphate starvation

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Genotypes:

FYPO:0008226 - increased total cellular phosphate (Pi) level

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Genotypes:

FYPO:0002061 - inviable vegetative cell population

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Genotypes:

FYPO:0003267 - normal acid phosphatase activity

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Genotypes:

FYPO:0002801 - normal protein degradation during nitrogen starvation

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Genotypes:

FYPO:0004083 - normal protein level

References:

Genotypes:

FYPO:0005572 - normal protein localization to cell periphery

References:

Genotypes:

FYPO:0002085 - normal vegetative cell growth

References:

Genotypes:

FYPO:0001357 - normal vegetative cell population growth

References:

Genotypes:

FYPO:0007629 - normal viability during G0

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Genotypes:

FYPO:0008229 - sensitive to phosphate (Pi)

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Genotypes:

FYPO:0000646 - swollen vegetative cell

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Genotypes:

FYPO:0002060 - viable vegetative cell population

References:

Genotypes:

Protein features

PBO:0111866 - SPX domain protein

PBO:0111749 - ubiquitin-protein ligase E3

PBO:0111746 - zf-C3HC4 type (RING finger)

PBO:0111743 - zinc finger protein

Qualitative gene expression

PomGeneEx:0000019 - protein level decreased

References:

PomGeneEx:0000027 - ribosomal density decreased

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PomGeneEx:0000011 - RNA level increased

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Quantitative gene expression

PBO:0006310 - protein level

References:

PBO:0011963 - RNA level

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Single locus phenotype

FYPO:0008228 - abnormal phosphate export

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Genotypes:

FYPO:0000121 - abnormal sporulation

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Genotypes:

FYPO:0005178 - abnormal vacuolar morphology during nitrogen starvation

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Genotypes:

FYPO:0004671 - abolished protein localization to vacuole during nitrogen starvation

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Genotypes:

FYPO:0002488 - cell lysis

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Genotypes:

FYPO:0001045 - decreased acid phosphatase activity

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Genotypes:

FYPO:0009091 - decreased cell population growth on lysine and proline nitrogen source

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Genotypes:

FYPO:0009092 - decreased cell population growth on lysine and serine nitrogen source

References:

Genotypes:

FYPO:0009073 - decreased cell population growth on lysine nitrogen source

References:

Genotypes:

FYPO:0000708 - decreased mating efficiency

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Genotypes:

FYPO:0002798 - decreased protein degradation during nitrogen starvation

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Genotypes:

FYPO:0001422 - decreased protein processing during vegetative growth

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Genotypes:

FYPO:0007818 - decreased protein ubiquitination during nitrogen starvation

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Genotypes:

FYPO:0008028 - decreased rate of acid phosphatase activation during phosphate starvation

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Genotypes:

FYPO:0006012 - elongated cell with abolished shmoo formation

References:

Genotypes:

FYPO:0007816 - increased cellular polyphosphate level

References:

Genotypes:

FYPO:0008226 - increased total cellular phosphate (Pi) level

References:

Genotypes:

FYPO:0004557 - increased vegetative cell population growth

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Genotypes:

FYPO:0007822 - large vacuoles during G0

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Genotypes:

FYPO:0006518 - loss of viability in G0

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Genotypes:

FYPO:0000245 - loss of viability in stationary phase

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Genotypes:

FYPO:0007817 - mislocalized nucleus during G0

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Genotypes:

FYPO:0004295 - multiseptate cell

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Genotypes:

FYPO:0007553 - normal G1 to G0 transition

References:

Genotypes:

FYPO:0002620 - normal growth on trichostatin A

References:

Genotypes:

FYPO:0002801 - normal protein degradation during nitrogen starvation

References:

Genotypes:

FYPO:0004083 - normal protein level

References:

Genotypes:

FYPO:0005572 - normal protein localization to cell periphery

References:

Genotypes:

FYPO:0006380 - normal protein localization to vacuole during nitrogen starvation

References:

Genotypes:

FYPO:0002085 - normal vegetative cell growth

References:

Genotypes:

FYPO:0001357 - normal vegetative cell population growth

References:

Genotypes:

FYPO:0007629 - normal viability during G0

References:

Genotypes:

FYPO:0000764 - resistance to cycloheximide

References:

Genotypes:

FYPO:0002693 - resistance to diamide

References:

Genotypes:

FYPO:0009038 - resistance to egtazic acid

References:

Genotypes:

FYPO:0003383 - resistance to tert-butyl hydroperoxide

References:

Genotypes:

FYPO:0004325 - sensitive to 5-fluorouracil

References:

Genotypes:

FYPO:0001097 - sensitive to amitrole

References:

Genotypes:

FYPO:0001501 - sensitive to brefeldin A

References:

Genotypes:

FYPO:0000096 - sensitive to cadmium

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Genotypes:

FYPO:0001245 - sensitive to cobalt

References:

Genotypes:

FYPO:0000104 - sensitive to cycloheximide

References:

Genotypes:

FYPO:0000842 - sensitive to ethanol during vegetative growth

References:

Genotypes:

FYPO:0001719 - sensitive to lithium

References:

Genotypes:

FYPO:0009084 - sensitive to lithium chloride and methyl methanesulfonate

References:

Genotypes:

FYPO:0009086 - sensitive to lithium chloride and sodium dodecyl sulfate

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Genotypes:

FYPO:0001214 - sensitive to potassium chloride

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Genotypes:

FYPO:0009082 - sensitive to potassium chloride and methyl methanesulfonate

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Genotypes:

FYPO:0007924 - sensitive to potassium chloride and sodium dodecyl sulfate

References:

Genotypes:

FYPO:0002617 - sensitive to sodium butyrate

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Genotypes:

FYPO:0005889 - sensitive to sodium chloride

References:

Genotypes:

FYPO:0009090 - sensitive to sodium chloride and sodium dodecyl sulfate

References:

Genotypes:

FYPO:0000086 - sensitive to tacrolimus

References:

Genotypes:

FYPO:0000115 - sensitive to valproic acid

References:

Genotypes:

FYPO:0003656 - sensitive to vanadate

References:

Genotypes:

FYPO:0002060 - viable vegetative cell population

References:

Genotypes:

FYPO:0002177 - viable vegetative cell with normal cell morphology

References:

Genotypes:

Taxonomic conservation

PBO:0011065 - conserved in eukaryotes

PBO:0011071 - conserved in eukaryotes only

PBO:0011064 - conserved in fungi

PBO:0000055 - no apparent S. cerevisiae ortholog

Warnings

PBO:0000070 - gene structure updated

References:

Protein features

IDNameInterPro nameDB name
PF00097zf-C3HC4Znf_C3HC4_RING-typePfam
PF03105SPXSPX_domPfam
cd23137RING-HC_TRY3-likeCDD
PS00518ZF_RING_1Znf_RING_CSPROSITE patterns
PS50089ZF_RING_2Znf_RINGPROSITE profiles
PS51382SPXSPX_domPROSITE profiles
SM00184RINGZnf_RINGSMART
G3DSA:3.30.40.10Znf_RING/FYVE/PHDCATH-Gene3D
SSF57850SUPERFAMILY
PTHR23327PANTHER
mobidb-lite-Disorderdisorder_predictionMobiDB-Disorder
mobidb-lite-Low-complexitydisorder_predictionMobiDB-Low-complexity

Orthologs

References / Literature

PMID:37949217 - Phosphate uptake restriction, phosphate export, and polyphosphate synthesis contribute synergistically to cellular proliferation and survival.
Takado M et al. J Biol Chem 2023 Nov 08;299(12):105454
PMID:21850271 - Genome-wide screening for genes associated with FK506 sensitivity in fission yeast.
Ma Y et al. PLoS One 2011;6(8):e23422
PMID:34250083 - Barcode sequencing and a high-throughput assay for chronological lifespan uncover ageing-associated genes in fission yeast.
Romila CA et al. Microb Cell 2021 Jul 05;8(7):146-160
PMID:33260998 - High-Throughput Flow Cytometry Combined with Genetic Analysis Brings New Insights into the Understanding of Chromatin Regulation of Cellular Quiescence.
Zahedi Y et al. Int J Mol Sci 2020 Nov 27;21(23)
PMID:22681890 - Hierarchical modularity and the evolution of genetic interactomes across species.
Ryan CJ et al. Mol Cell 2012 Jun 08;46(5):691-704
PMID:12529438 - Global transcriptional responses of fission yeast to environmental stress.
Chen D et al. Mol Biol Cell 2003 Jan;14(1):214-29
PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.
Marguerat S et al. Cell 2012 Oct 26;151(3):671-83
PMID:28974540 - The fission yeast nucleoporin Alm1 is required for proteasomal degradation of kinetochore components.
Salas-Pino S et al. J Cell Biol 2017 Nov 06;216(11):3591-3608
PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.
Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623
PMID:37787768 - Broad functional profiling of fission yeast proteins using phenomics and machine learning.
Rodríguez-López M et al. Elife 2023 Oct 03;12
GO_REF:0000002 - Comments
PMID:26412298 - A Degenerate Cohort of Yeast Membrane Trafficking DUBs Mediates Cell Polarity and Survival.
Beckley JR et al. Mol Cell Proteomics 2015 Dec;14(12):3132-41
PMID:37970674 - SUMOylation regulates Lem2 function in centromere clustering and silencing.
Strachan J et al. J Cell Sci 2023 Dec 01;136(23)
PMID:38899862 - Suppression of inositol pyrophosphate toxicosis and hyper-repression of the fission yeast PHO regulon by loss-of-function mutations in chromatin remodelers Snf22 and Sol1.
Schwer B et al. mBio 2024 Jun 20;:e0125224
PMID:36882296 - Duf89 abets lncRNA control of fission yeast phosphate homeostasis via its antagonism of precocious lncRNA transcription termination.
Sanchez AM et al. RNA 2023 Jun;29(6):808-825
PMID:27984744 - Survival in Quiescence Requires the Euchromatic Deployment of Clr4/SUV39H by Argonaute-Associated Small RNAs.
Joh RI et al. Mol Cell 2016 Dec 15;64(6):1088-1101
PMID:24763107 - Absolute proteome and phosphoproteome dynamics during the cell cycle of Schizosaccharomyces pombe (Fission Yeast).
Carpy A et al. Mol Cell Proteomics 2014 Aug;13(8):1925-36
PMID:37772819 - Activities, substrate specificity, and genetic interactions of fission yeast Siw14, a cysteinyl-phosphatase-type inositol pyrophosphatase.
Sanchez AM et al. mBio 2023 Sep 29;14(5):e0205623
PMID:28410370 - A systematic screen for morphological abnormalities during fission yeast sexual reproduction identifies a mechanism of actin aster formation for cell fusion.
Dudin O et al. PLoS Genet 2017 Apr;13(4):e1006721
PMID:23163955 - Analysis of stress-induced duplex destabilization (SIDD) properties of replication origins, genes and intergenes in the fission yeast, Schizosaccharomyces pombe.
Yadav MP et al. BMC Res Notes 2012 Nov 19;5:643
PMID:35012333 - Cleavage-Polyadenylation Factor Cft1 and SPX Domain Proteins Are Agents of Inositol Pyrophosphate Toxicosis in Fission Yeast.
Schwer B et al. mBio 2022 Feb 22;13(1):e0347621
PMID:22806344 - Genome-wide screen reveals novel mechanisms for regulating cobalt uptake and detoxification in fission yeast.
Ryuko S et al. Mol Genet Genomics 2012 Aug;287(8):651-62
PMID:23861937 - Genome-wide screening for genes associated with valproic acid sensitivity in fission yeast.
Zhang L et al. PLoS One 2013;8(7):e68738
PMID:25483073 - Chromosome segregation and organization are targets of 5'-Fluorouracil in eukaryotic cells.
Mojardín L et al. Cell Cycle 2015;14(2):206-18
PMID:16823372 - ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe.
Matsuyama A et al. Nat Biotechnol 2006 Jul;24(7):841-7
PMID:33313903 - Ribosome profiling reveals ribosome stalling on tryptophan codons and ribosome queuing upon oxidative stress in fission yeast.
Rubio A et al. Nucleic Acids Res 2021 Jan 11;49(1):383-399
PMID:39705284 - Proteomic and phosphoproteomic analyses reveal that TORC1 is reactivated by pheromone signaling during sexual reproduction in fission yeast.
Bérard M et al. PLoS Biol 2024 Dec 20;22(12):e3002963
PMID:21511999 - Comparative functional genomics of the fission yeasts.
Rhind N et al. Science 2011 May 20;332(6032):930-6
PMID:29259000 - Genes Important for Schizosaccharomyces pombe Meiosis Identified Through a Functional Genomics Screen.
Blyth J et al. Genetics 2018 Feb;208(2):589-603
PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.
Hayles J et al. Open Biol 2013 May 22;3(5):130053
PMID:40015273 - A comprehensive Schizosaccharomyces pombe atlas of physical transcription factor interactions with proteins and chromatin.
Skribbe M et al. Mol Cell 2025 Feb 19;
PMID:34147496 - Regulation of inorganic polyphosphate is required for proper vacuolar proteolysis in fission yeast.
Sawada N et al. J Biol Chem 2021 Jul;297(1):100891