PomBase home

protein coding gene - spp1 (SPAC6B12.10c) - DNA primase catalytic subunit Spp1

Gene summary

Standard name
spp1
Systematic ID
SPAC6B12.10c
Product
DNA primase catalytic subunit Spp1
Organism
Schizosaccharomyces pombe (fission yeast)
Synonyms
pri1
UniProt ID
O14215
ORFeome ID
23/23C12
Characterisation status
biological role published
Feature type
mRNA gene
Genomic location
chromosome I: 2428976..2430635 reverse strand

Annotation

Disease association

MONDO:0859276 - primordial dwarfism-immunodeficiency-lipodystrophy syndrome

References:

GO biological process

GO:0006269 - DNA replication, synthesis of primer

References:

GO cellular component

GO:0005658 - alpha DNA polymerase:primase complex

References:

GO:0000785 - chromatin

References:

GO:0005737 - cytoplasm

References:

GO:0005634 - nucleus

References:

GO molecular function

GO:0003899 - DNA-directed RNA polymerase activity

References:

GO:0008233 - peptidase activity

References:

Multi-locus phenotype

FYPO:0000062 - abnormal nuclear morphology during vegetative growth

References:

Genotypes:

FYPO:0000229 - cut

References:

Genotypes:

FYPO:0002019 - elongated telomeres during vegetative growth

References:

Genotypes:

FYPO:0000951 - inviable small vegetative cell

References:

Genotypes:

FYPO:0002061 - inviable vegetative cell population

References:

Genotypes:

FYPO:0002060 - viable vegetative cell population

References:

Genotypes:

Qualitative gene expression

PomGeneEx:0000023 - protein level constant

References:

PomGeneEx:0000019 - protein level decreased

References:

Quantitative gene expression

PBO:0006310 - protein level

References:

PBO:0011963 - RNA level

References:

Single locus phenotype

FYPO:0000611 - abnormal cell cycle arrest in mitotic S phase

References:

Genotypes:

FYPO:0000059 - abnormal mitotic cell cycle

References:

Genotypes:

FYPO:0000444 - abnormal mitotic cell cycle arrest with replicated DNA

References:

Genotypes:

FYPO:0000062 - abnormal nuclear morphology during vegetative growth

References:

Genotypes:

FYPO:0000705 - abolished protein-protein interaction

References:

Genotypes:

FYPO:0003165 - cut with abnormal chromosome segregation

References:

Genotypes:

FYPO:0000470 - decreased mating type switching

References:

Genotypes:

FYPO:0001324 - decreased protein level during vegetative growth

References:

Genotypes:

FYPO:0001838 - decreased protein phosphorylation during vegetative growth

References:

Genotypes:

FYPO:0001645 - decreased protein-protein interaction

References:

Genotypes:

FYPO:0004372 - decreased response to mitotic G2 DNA damage checkpoint signaling

References:

Genotypes:

FYPO:0001355 - decreased vegetative cell population growth

References:

Genotypes:

FYPO:0002019 - elongated telomeres during vegetative growth

References:

Genotypes:

FYPO:0002700 - increased protein kinase activity

References:

Genotypes:

FYPO:0000314 - inviable after spore germination with elongated germ tube

References:

Genotypes:

FYPO:0002262 - inviable after spore germination, multiple cell divisions, elongated cell

References:

Genotypes:

FYPO:0001042 - inviable after spore germination, single or double cell division

References:

Genotypes:

FYPO:0003763 - inviable aseptate mononucleate vegetative cell

References:

Genotypes:

FYPO:0004255 - inviable elongated mononucleate vegetative cell

References:

Genotypes:

FYPO:0001490 - inviable elongated vegetative cell

References:

Genotypes:

FYPO:0002061 - inviable vegetative cell population

References:

Genotypes:

FYPO:0001387 - loss of viability at high temperature

References:

Genotypes:

FYPO:0003555 - normal chromatin silencing at subtelomere

References:

Genotypes:

FYPO:0003353 - normal DNA double-strand break formation at mating-type locus

References:

Genotypes:

FYPO:0001357 - normal vegetative cell population growth

References:

Genotypes:

Taxonomic conservation

PBO:0011072 - conserved in archaea

PBO:0011067 - conserved in bacteria

PBO:0011065 - conserved in eukaryotes

PBO:0011064 - conserved in fungi

PBO:0011069 - conserved in metazoa

PBO:0011070 - conserved in vertebrates

PBO:0006222 - predominantly single copy (one to one)

Protein features

IDNameInterPro nameDB name
PF01896DNA_primase_SDNA_primase_SPFAM
cd04860AE_Prim_SDNA_primase_ssu_euk/arcCDD
G3DSA:3.90.920.10:FF:000002FUNFAM
SSF56747Prim-pol domainSUPERFAMILY
G3DSA:3.90.920.10DNA primase, PRIM domainGENE3D
PTHR10536DNA PRIMASE SMALL SUBUNITPANTHER
mobidb-lite-Disorderdisorder_predictionMOBIDB-Disorder
mobidb-lite-Polardisorder_predictionMOBIDB-Polar
TIGR00335primase_smlDNA_primase_ssu_euk/arcNCBIFAM

Orthologs

References / Literature

PMID:11160827 - Role of fission yeast primase catalytic subunit in the replication checkpoint.
Griffiths DJ et al. Mol Biol Cell 2001 Jan;12(1):115-28
PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.
Marguerat S et al. Cell 2012 Oct 26;151(3):671-83
PMID:24713849 - Post-transcriptional regulation of meiotic genes by a nuclear RNA silencing complex.
Egan ED et al. RNA 2014 Jun;20(6):867-81
PMID:15507118 - An interactive gene network for securin-separase, condensin, cohesin, Dis1/Mtc1 and histones constructed by mass transformation.
Yuasa T et al. Genes Cells 2004 Nov;9(11):1069-82
PMID:39705284 - Proteomic and phosphoproteomic analyses reveal that TORC1 is reactivated by pheromone signaling during sexual reproduction in fission yeast.
Bérard M et al. PLoS Biol 2024 Dec 20;22(12):e3002963
GO_REF:0000002 - Comments
PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.
Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623
PMID:15314153 - The B-subunit of DNA polymerase alpha-primase associates with the origin recognition complex for initiation of DNA replication.
Uchiyama M et al. Mol Cell Biol 2004 Sep;24(17):7419-34
PMID:11344166 - Essential role of Sna41/Cdc45 in loading of DNA polymerase alpha onto minichromosome maintenance proteins in fission yeast.
Uchiyama M et al. J Biol Chem 2001 Jul 13;276(28):26189-96
GO_REF:0000033 - Annotation inferences using phylogenetic trees
PMID:30759238 - Role of Cdc23/Mcm10 in generating the ribonucleotide imprint at the mat1 locus in fission yeast.
Singh B et al. Nucleic Acids Res 2019 Apr 23;47(7):3422-3433
PMID:12697806 - Replication proteins influence the maintenance of telomere length and telomerase protein stability.
Dahlén M et al. Mol Cell Biol 2003 May;23(9):3031-42
PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.
Hayles J et al. Open Biol 2013 May 22;3(5):130053
PMID:12589755 - The fission yeast spSet1p is a histone H3-K4 methyltransferase that functions in telomere maintenance and DNA repair in an ATM kinase Rad3-dependent pathway.
Kanoh J et al. J Mol Biol 2003 Feb 28;326(4):1081-94
PMID:30796050 - Ssu72 phosphatase is a conserved telomere replication terminator.
Escandell JM et al. EMBO J 2019 Apr 01;38(7)
PMID:24763107 - Absolute proteome and phosphoproteome dynamics during the cell cycle of Schizosaccharomyces pombe (Fission Yeast).
Carpy A et al. Mol Cell Proteomics 2014 Aug;13(8):1925-36
PMID:39094570 - A replisome-associated histone H3-H4 chaperone required for epigenetic inheritance.
Yu J et al. Cell 2024 Sep 05;187(18):5010-5028.e24
PMID:21511999 - Comparative functional genomics of the fission yeasts.
Rhind N et al. Science 2011 May 20;332(6032):930-6
PMID:11027257 - Analysis of fission yeast primase defines the checkpoint responses to aberrant S phase initiation.
Tan S et al. Mol Cell Biol 2000 Nov;20(21):7853-66
PMID:16823372 - ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe.
Matsuyama A et al. Nat Biotechnol 2006 Jul;24(7):841-7
PMID:15941470 - Nuclear distribution and chromatin association of DNA polymerase alpha-primase is affected by TEV protease cleavage of Cdc23 (Mcm10) in fission yeast.
Yang X et al. BMC Mol Biol 2005 Jun 07;6:13
PB_REF:0000006 - Disease associations from Monarch via human-pombe orthologs
PMID:12399381 - The sal3(+) gene encodes an importin-beta implicated in the nuclear import of Cdc25 in Schizosaccharomyces pombe.
Chua G et al. Genetics 2002 Oct;162(2):689-703