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protein coding gene - spp1 (SPAC6B12.10c) - DNA primase catalytic subunit Spp1

Gene summary

Standard name
spp1
Systematic ID
SPAC6B12.10c
Product
DNA primase catalytic subunit Spp1
Organism
Schizosaccharomyces pombe (fission yeast)
Synonyms
pri1
UniProt ID
O14215
ORFeome ID
23/23C12
Characterisation status
biological role published
Feature type
mRNA gene
Genomic location
chromosome I: 2428976..2430635 reverse strand

Annotation

Disease association

MONDO:0859276 - primordial dwarfism-immunodeficiency-lipodystrophy syndrome

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GO biological process

GO:0006269 - DNA replication, synthesis of primer

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GO cellular component

GO:0005658 - alpha DNA polymerase:primase complex

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GO:0000785 - chromatin

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GO:0005737 - cytoplasm

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GO:0005634 - nucleus

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GO molecular function

GO:0003899 - DNA-directed RNA polymerase activity

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GO:0008233 - peptidase activity

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Multi-locus phenotype

FYPO:0000062 - abnormal nuclear morphology during vegetative growth

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Genotypes:

FYPO:0000229 - cut

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Genotypes:

FYPO:0002019 - elongated telomeres during vegetative growth

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Genotypes:

FYPO:0000951 - inviable small vegetative cell

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Genotypes:

FYPO:0002061 - inviable vegetative cell population

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Genotypes:

FYPO:0002060 - viable vegetative cell population

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Genotypes:

Qualitative gene expression

PomGeneEx:0000023 - protein level constant

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PomGeneEx:0000019 - protein level decreased

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Quantitative gene expression

PBO:0006310 - protein level

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PBO:0011963 - RNA level

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Single locus phenotype

FYPO:0000611 - abnormal cell cycle arrest in mitotic S phase

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Genotypes:

FYPO:0000059 - abnormal mitotic cell cycle

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Genotypes:

FYPO:0000444 - abnormal mitotic cell cycle arrest with replicated DNA

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Genotypes:

FYPO:0000062 - abnormal nuclear morphology during vegetative growth

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Genotypes:

FYPO:0000705 - abolished protein-protein interaction

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Genotypes:

FYPO:0003165 - cut with abnormal chromosome segregation

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Genotypes:

FYPO:0000470 - decreased mating type switching

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Genotypes:

FYPO:0001324 - decreased protein level during vegetative growth

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Genotypes:

FYPO:0001838 - decreased protein phosphorylation during vegetative growth

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Genotypes:

FYPO:0001645 - decreased protein-protein interaction

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Genotypes:

FYPO:0004372 - decreased response to mitotic G2 DNA damage checkpoint signaling

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Genotypes:

FYPO:0001355 - decreased vegetative cell population growth

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Genotypes:

FYPO:0002019 - elongated telomeres during vegetative growth

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Genotypes:

FYPO:0002700 - increased protein kinase activity

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Genotypes:

FYPO:0000314 - inviable after spore germination with elongated germ tube

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Genotypes:

FYPO:0002262 - inviable after spore germination, multiple cell divisions, elongated cell

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Genotypes:

FYPO:0001042 - inviable after spore germination, single or double cell division

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Genotypes:

FYPO:0003763 - inviable aseptate mononucleate vegetative cell

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Genotypes:

FYPO:0004255 - inviable elongated mononucleate vegetative cell

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Genotypes:

FYPO:0001490 - inviable elongated vegetative cell

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Genotypes:

FYPO:0002061 - inviable vegetative cell population

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Genotypes:

FYPO:0001387 - loss of viability at high temperature

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Genotypes:

FYPO:0003555 - normal chromatin silencing at subtelomere

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Genotypes:

FYPO:0003353 - normal DNA double-strand break formation at mating-type locus

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Genotypes:

FYPO:0001357 - normal vegetative cell population growth

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Genotypes:

Taxonomic conservation

PBO:0011072 - conserved in archaea

PBO:0011067 - conserved in bacteria

PBO:0011065 - conserved in eukaryotes

PBO:0011064 - conserved in fungi

PBO:0011069 - conserved in metazoa

PBO:0011070 - conserved in vertebrates

PBO:0006222 - predominantly single copy (one to one)

Protein features

IDNameInterPro nameDB name
PF01896DNA_primase_SDNA_primase_SPfam
cd04860AE_Prim_SDNA_primase_ssu_euk/arcCDD
G3DSA:3.90.920.10:FF:000002CATH-FunFam
G3DSA:3.90.920.10CATH-Gene3D
SSF56747SUPERFAMILY
PTHR10536PANTHER
TIGR00335primase_smlDNA_primase_ssu_euk/arcNCBIFAM
mobidb-lite-Disorderdisorder_predictionMobiDB-Disorder
mobidb-lite-Polardisorder_predictionMobiDB-Polar

Orthologs

References / Literature

GO_REF:0000002 - Comments
PMID:11344166 - Essential role of Sna41/Cdc45 in loading of DNA polymerase alpha onto minichromosome maintenance proteins in fission yeast.
Uchiyama M et al. J Biol Chem 2001 Jul 13;276(28):26189-96
PB_REF:0000006 - Disease associations from Monarch via human-pombe orthologs
PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.
Marguerat S et al. Cell 2012 Oct 26;151(3):671-83
PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.
Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623
PMID:15941470 - Nuclear distribution and chromatin association of DNA polymerase alpha-primase is affected by TEV protease cleavage of Cdc23 (Mcm10) in fission yeast.
Yang X et al. BMC Mol Biol 2005 Jun 07;6:13
PMID:12589755 - The fission yeast spSet1p is a histone H3-K4 methyltransferase that functions in telomere maintenance and DNA repair in an ATM kinase Rad3-dependent pathway.
Kanoh J et al. J Mol Biol 2003 Feb 28;326(4):1081-94
PMID:12399381 - The sal3(+) gene encodes an importin-beta implicated in the nuclear import of Cdc25 in Schizosaccharomyces pombe.
Chua G et al. Genetics 2002 Oct;162(2):689-703
PMID:16823372 - ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe.
Matsuyama A et al. Nat Biotechnol 2006 Jul;24(7):841-7
PMID:39094570 - A replisome-associated histone H3-H4 chaperone required for epigenetic inheritance.
Yu J et al. Cell 2024 Sep 05;187(18):5010-5028.e24
PMID:39705284 - Proteomic and phosphoproteomic analyses reveal that TORC1 is reactivated by pheromone signaling during sexual reproduction in fission yeast.
Bérard M et al. PLoS Biol 2024 Dec 20;22(12):e3002963
PMID:11027257 - Analysis of fission yeast primase defines the checkpoint responses to aberrant S phase initiation.
Tan S et al. Mol Cell Biol 2000 Nov;20(21):7853-66
PMID:30759238 - Role of Cdc23/Mcm10 in generating the ribonucleotide imprint at the mat1 locus in fission yeast.
Singh B et al. Nucleic Acids Res 2019 Apr 23;47(7):3422-3433
GO_REF:0000033 - Annotation inferences using phylogenetic trees
PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.
Hayles J et al. Open Biol 2013 May 22;3(5):130053
PMID:24713849 - Post-transcriptional regulation of meiotic genes by a nuclear RNA silencing complex.
Egan ED et al. RNA 2014 Jun;20(6):867-81
PMID:24763107 - Absolute proteome and phosphoproteome dynamics during the cell cycle of Schizosaccharomyces pombe (Fission Yeast).
Carpy A et al. Mol Cell Proteomics 2014 Aug;13(8):1925-36
PMID:15507118 - An interactive gene network for securin-separase, condensin, cohesin, Dis1/Mtc1 and histones constructed by mass transformation.
Yuasa T et al. Genes Cells 2004 Nov;9(11):1069-82
PMID:12697806 - Replication proteins influence the maintenance of telomere length and telomerase protein stability.
Dahlén M et al. Mol Cell Biol 2003 May;23(9):3031-42
PMID:15314153 - The B-subunit of DNA polymerase alpha-primase associates with the origin recognition complex for initiation of DNA replication.
Uchiyama M et al. Mol Cell Biol 2004 Sep;24(17):7419-34
PMID:21511999 - Comparative functional genomics of the fission yeasts.
Rhind N et al. Science 2011 May 20;332(6032):930-6
PMID:30796050 - Ssu72 phosphatase is a conserved telomere replication terminator.
Escandell JM et al. EMBO J 2019 Apr 01;38(7)
PMID:11160827 - Role of fission yeast primase catalytic subunit in the replication checkpoint.
Griffiths DJ et al. Mol Biol Cell 2001 Jan;12(1):115-28