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protein coding gene - drc1 (SPAC6B12.11) - DNA replication preinitiation complex assembly protein

Gene summary

Standard name
drc1
Systematic ID
SPAC6B12.11
Product
DNA replication preinitiation complex assembly protein
Organism
Schizosaccharomyces pombe (fission yeast)
Synonyms
sld2
UniProt ID
O14216
ORFeome ID
17/17D11
Characterisation status
biological role published
Feature type
mRNA gene
Genomic location
chromosome I: 2430789..2432838 forward strand

Annotation

Disease association

MONDO:0009039 - Baller-Gerold syndrome

References:

MONDO:0009955 - rapadilino syndrome

References:

MONDO:0016369 - Rothmund-Thomson syndrome type 2

References:

GO biological process

GO:1902975 - mitotic DNA replication initiation

References:

GO:1902977 - mitotic DNA replication preinitiation complex assembly

References:

GO cellular component

GO:0043596 - nuclear replication fork

References:

GO:0005634 - nucleus

References:

Modification

MOD:00046 - O-phospho-L-serine

References:

MOD:00047 - O-phospho-L-threonine

References:

MOD:00696 - phosphorylated residue

References:

Multi-locus phenotype

FYPO:0001645 - decreased protein-protein interaction

References:

Genotypes:

FYPO:0000703 - normal protein-protein interaction

References:

Genotypes:

FYPO:0001357 - normal vegetative cell population growth

References:

Genotypes:

Quantitative gene expression

PBO:0011963 - RNA level

References:

Single locus phenotype

FYPO:0000444 - abnormal mitotic cell cycle arrest with replicated DNA

References:

Genotypes:

FYPO:0001343 - abnormal mitotic DNA replication

References:

Genotypes:

FYPO:0005095 - abolished protein localization to chromatin at replication origin

References:

Genotypes:

FYPO:0000705 - abolished protein-protein interaction

References:

Genotypes:

FYPO:0003165 - cut with abnormal chromosome segregation

References:

Genotypes:

FYPO:0001355 - decreased vegetative cell population growth

References:

Genotypes:

FYPO:0000453 - DNA content decreased during vegetative growth

References:

Genotypes:

FYPO:0001122 - elongated vegetative cell

References:

Genotypes:

FYPO:0000311 - inviable after spore germination with normal, unseptated germ tube morphology

References:

Genotypes:

FYPO:0001490 - inviable elongated vegetative cell

References:

Genotypes:

FYPO:0002151 - inviable spore

References:

Genotypes:

FYPO:0002061 - inviable vegetative cell population

References:

Genotypes:

FYPO:0000284 - large and small daughter nuclei, with unequal mitotic sister chromatid segregation

References:

Genotypes:

FYPO:0001387 - loss of viability at high temperature

References:

Genotypes:

FYPO:0000703 - normal protein-protein interaction

References:

Genotypes:

FYPO:0001357 - normal vegetative cell population growth

References:

Genotypes:

Taxonomic conservation

PBO:0011065 - conserved in eukaryotes

PBO:0011071 - conserved in eukaryotes only

PBO:0011064 - conserved in fungi

PBO:0011069 - conserved in metazoa

PBO:0011070 - conserved in vertebrates

PBO:0006222 - predominantly single copy (one to one)

Protein features

IDNameInterPro nameDB name
PF11719Drc1-Sld2DNA_rep_checkpnt_proteinPfam
cd22289RecQL4_SLD2_NTDCDD
G3DSA:1.10.10.1460:FF:000001CATH-FunFam
G3DSA:1.10.10.1460CATH-Gene3D
PTHR28124Sld2PANTHER
CoilCOILS
mobidb-lite-Disorderdisorder_predictionMobiDB-Disorder
mobidb-lite-Negative-Polyelectrolytedisorder_predictionMobiDB-Negative-Polyelectrolyte

Orthologs

References / Literature

PMID:22718908 - DNA polymerization-independent functions of DNA polymerase epsilon in assembly and progression of the replisome in fission yeast.
Handa T et al. Mol Biol Cell 2012 Aug;23(16):3240-53
PMID:27984725 - CDK Substrate Phosphorylation and Ordering the Cell Cycle.
Swaffer MP et al. Cell 2016 Dec 15;167(7):1750-1761.e16
PMID:27298342 - Identification of S-phase DNA damage-response targets in fission yeast reveals conservation of damage-response networks.
Willis NA et al. Proc Natl Acad Sci U S A 2016 Jun 28;113(26):E3676-85
PMID:25720772 - Quantitative phosphoproteomics reveals pathways for coordination of cell growth and division by the conserved fission yeast kinase pom1.
Kettenbach AN et al. Mol Cell Proteomics 2015 May;14(5):1275-87
PMID:18257517 - Phosphoproteome analysis of fission yeast.
Wilson-Grady JT et al. J Proteome Res 2008 Mar;7(3):1088-97
PMID:29996109 - Quantitative Phosphoproteomics Reveals the Signaling Dynamics of Cell-Cycle Kinases in the Fission Yeast Schizosaccharomyces pombe.
Swaffer MP et al. Cell Rep 2018 Jul 10;24(2):503-514
PMID:30726745 - Fission Yeast NDR/LATS Kinase Orb6 Regulates Exocytosis via Phosphorylation of the Exocyst Complex.
Tay YD et al. Cell Rep 2019 Feb 05;26(6):1654-1667.e7
PMID:33823663 - A TOR (target of rapamycin) and nutritional phosphoproteome of fission yeast reveals novel targets in networks conserved in humans.
Halova L et al. Open Biol 2021 Apr;11(4):200405
PMID:21593208 - CDK promotes interactions of Sld3 and Drc1 with Cut5 for initiation of DNA replication in fission yeast.
Fukuura M et al. Mol Biol Cell 2011 Jul 15;22(14):2620-33
PMID:39476757 - Characterization of Ksg1 protein kinase-dependent phosphoproteome in the fission yeast S. pombe.
Cipak L et al. Biochem Biophys Res Commun 2024 Oct 25;736:150895
PMID:21712547 - Mitotic substrates of the kinase aurora with roles in chromatin regulation identified through quantitative phosphoproteomics of fission yeast.
Koch A et al. Sci Signal 2011 Jun 28;4(179):rs6
PMID:21945095 - Mcm10 interacts with Rad4/Cut5(TopBP1) and its association with origins of DNA replication is dependent on Rad4/Cut5(TopBP1).
Taylor M et al. DNA Repair (Amst) 2011 Nov 10;10(11):1154-63
PMID:39705284 - Proteomic and phosphoproteomic analyses reveal that TORC1 is reactivated by pheromone signaling during sexual reproduction in fission yeast.
Bérard M et al. PLoS Biol 2024 Dec 20;22(12):e3002963
PB_REF:0000006 - Disease associations from Monarch via human-pombe orthologs
PMID:21511999 - Comparative functional genomics of the fission yeasts.
Rhind N et al. Science 2011 May 20;332(6032):930-6
PMID:22681890 - Hierarchical modularity and the evolution of genetic interactomes across species.
Ryan CJ et al. Mol Cell 2012 Jun 08;46(5):691-704
PMID:16990792 - Ordered assembly of Sld3, GINS and Cdc45 is distinctly regulated by DDK and CDK for activation of replication origins.
Yabuuchi H et al. EMBO J 2006 Oct 04;25(19):4663-74
PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.
Hayles J et al. Open Biol 2013 May 22;3(5):130053
GO_REF:0000024 - Manual transfer of experimentally-verified manual GO annotation data to orthologs by curator judgment of sequence similarity.
PMID:22433840 - Mcm10 plays an essential role in origin DNA unwinding after loading of the CMG components.
Kanke M et al. EMBO J 2012 May 02;31(9):2182-94
PMID:39367033 - Quantitative proteomics and phosphoproteomics profiling of meiotic divisions in the fission yeast Schizosaccharomyces pombe.
Sivakova B et al. Sci Rep 2024 Oct 04;14(1):23105
PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.
Marguerat S et al. Cell 2012 Oct 26;151(3):671-83
PMID:28218250 - Chromatin remodeller Fun30 Fft3 induces nucleosome disassembly to facilitate RNA polymerase II elongation.
Lee J et al. Nat Commun 2017 Feb 20;8:14527
PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.
Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623
PMID:24763107 - Absolute proteome and phosphoproteome dynamics during the cell cycle of Schizosaccharomyces pombe (Fission Yeast).
Carpy A et al. Mol Cell Proteomics 2014 Aug;13(8):1925-36
PMID:11937031 - CDK phosphorylation of Drc1 regulates DNA replication in fission yeast.
Noguchi E et al. Curr Biol 2002 Apr 02;12(7):599-605