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protein coding gene - tnr3 (SPAC6F12.05c) - thiamine diphosphokinase Tnr3/ Nudix hydrolase fusion protein

Gene summary

Standard name
tnr3
Systematic ID
SPAC6F12.05c
Product
thiamine diphosphokinase Tnr3/ Nudix hydrolase fusion protein
Organism
Schizosaccharomyces pombe (fission yeast)
UniProt ID
P41888
ORFeome ID
35/35E08
Characterisation status
biological role published
Feature type
mRNA gene
Genomic location
chromosome I: 1316981..1318978 reverse strand

Annotation

Catalytic activity attributes

PBO:0091320 - protein substrate KM, ATP KM 1.9 mM

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PBO:0091321 - protein substrate KM, Mg2+ KM 3.0 mM

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PBO:0091322 - protein substrate KM, thiamine KM 6 uM

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Complementation

PBO:0011086 - functionally complemented by S. cerevisiae THI80

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Disease association

MONDO:0013761 - childhood encephalopathy due to thiamine pyrophosphokinase deficiency

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GO biological process

GO:0009228 - thiamine biosynthetic process

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GO:0009229 - thiamine diphosphate biosynthetic process

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GO:0006772 - thiamine metabolic process

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GO:0036172 - thiamine salvage

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GO cellular component

GO:0005737 - cytoplasm

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GO:0005829 - cytosol

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GO molecular function

GO:0044715 - 8-oxo-dGDP phosphatase activity

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GO:0005524 - ATP binding

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GO:0030975 - thiamine binding

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GO:0004788 - thiamine diphosphokinase activity

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Genome organisation

PBO:0000229 - tandem fusion protein

Modification

MOD:00046 - O-phospho-L-serine

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MOD:00047 - O-phospho-L-threonine

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Multi-locus phenotype

FYPO:0000080 - decreased cell population growth at low temperature

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Genotypes:

FYPO:0002243 - increased acid phosphatase activity

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Genotypes:

FYPO:0003267 - normal acid phosphatase activity

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Genotypes:

Quantitative gene expression

PBO:0006310 - protein level

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PBO:0011963 - RNA level

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Single locus phenotype

FYPO:0001118 - abnormal vegetative cell morphology

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Genotypes:

FYPO:0000708 - decreased mating efficiency

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Genotypes:

FYPO:0001040 - decreased thiamine diphosphokinase activity

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Genotypes:

FYPO:0002243 - increased acid phosphatase activity

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Genotypes:

FYPO:0001041 - increased cellular thiamine level

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Genotypes:

FYPO:0001043 - increased mating efficiency

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Genotypes:

FYPO:0001243 - increased RNA level during cellular response to thiamine

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Genotypes:

FYPO:0000825 - increased RNA level during vegetative growth

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Genotypes:

FYPO:0001042 - inviable after spore germination, single or double cell division

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Genotypes:

FYPO:0002187 - inviable normal volume spheroid vegetative cell

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Genotypes:

FYPO:0002061 - inviable vegetative cell population

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Genotypes:

FYPO:0001234 - slow vegetative cell population growth

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Genotypes:

Taxonomic conservation

PBO:0011065 - conserved in eukaryotes

PBO:0011064 - conserved in fungi

PBO:0011069 - conserved in metazoa

PBO:0011070 - conserved in vertebrates

Protein features

IDNameInterPro nameDB name
PF00293NUDIXNUDIX_hydrolase_domPfam
PF04263TPK_catalyticTPK_catalyticPfam
PF04265TPK_B1_bindingThiamin_PyroPKinase_B1-bdPfam
PF15916DUF4743DUF4743Pfam
cd03676NUDIX_Tnr3_likeCDD
cd07995TPKThi_PPkinaseCDD
PS51462NUDIXNUDIX_hydrolase_domPROSITE profiles
SM00983TPK_B1_bindingThiamin_PyroPKinase_B1-bdSMART
G3DSA:3.90.79.10:FF:000019CATH-FunFam
G3DSA:3.40.50.10240TPK_catalytic_sfCATH-Gene3D
G3DSA:3.90.79.10NUDIX_hydrolase-like_dom_sfCATH-Gene3D
SSF55811NUDIX_hydrolase-like_dom_sfSUPERFAMILY
SSF63862TPK_B1-bd_sfSUPERFAMILY
SSF63999TPK_catalytic_sfSUPERFAMILY
PTHR13622PANTHER
TIGR01378thi_PPkinaseThi_PPkinaseNCBIFAM

Orthologs

References / Literature

PMID:24763107 - Absolute proteome and phosphoproteome dynamics during the cell cycle of Schizosaccharomyces pombe (Fission Yeast).
Carpy A et al. Mol Cell Proteomics 2014 Aug;13(8):1925-36
PMID:25720772 - Quantitative phosphoproteomics reveals pathways for coordination of cell growth and division by the conserved fission yeast kinase pom1.
Kettenbach AN et al. Mol Cell Proteomics 2015 May;14(5):1275-87
PMID:1551569 - Isolation and characterization of regulatory mutants from Schizosaccharomyces pombe involved in thiamine-regulated gene expression.
Schweingruber AM et al. Genetics 1992 Mar;130(3):445-9
PMID:16823372 - ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe.
Matsuyama A et al. Nat Biotechnol 2006 Jul;24(7):841-7
GO_REF:0000033 - Annotation inferences using phylogenetic trees
PMID:21340088 - Microarray-based target identification using drug hypersensitive fission yeast expressing ORFeome.
Arita Y et al. Mol Biosyst 2011 May;7(5):1463-72
PMID:21511999 - Comparative functional genomics of the fission yeasts.
Rhind N et al. Science 2011 May 20;332(6032):930-6
GO_REF:0000002 - Comments
PMID:1644306 - Cloning and regulation of Schizosaccharomyces pombe thi2, a gene involved in thiamine biosynthesis.
Zurlinden A et al. Gene 1992 Aug 01;117(1):141-3
PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.
Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623
PMID:23834287 - A cross-kingdom Nudix enzyme that pre-empts damage in thiamin metabolism.
Goyer A et al. Biochem J 2013 Sep 15;454(3):533-42
PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.
Hayles J et al. Open Biol 2013 May 22;3(5):130053
PB_REF:0000006 - Disease associations from Monarch via human-pombe orthologs
PMID:34967420 - Genetic screen for suppression of transcriptional interference reveals fission yeast 14-3-3 protein Rad24 as an antagonist of precocious Pol2 transcription termination.
Garg A et al. Nucleic Acids Res 2022 Jan 25;50(2):803-819
PMID:7499352 - Schizosaccharomyces pombe thiamine pyrophosphokinase is encoded by gene tnr3 and is a regulator of thiamine metabolism, phosphate metabolism, mating, and growth.
Fankhauser H et al. J Biol Chem 1995 Nov 24;270(47):28457-62
PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.
Marguerat S et al. Cell 2012 Oct 26;151(3):671-83
PMID:7961415 - Cloning, nucleotide sequence, and regulation of Schizosaccharomyces pombe thi4, a thiamine biosynthetic gene.
Zurlinden A et al. J Bacteriol 1994 Nov;176(21):6631-5
PMID:30726745 - Fission Yeast NDR/LATS Kinase Orb6 Regulates Exocytosis via Phosphorylation of the Exocyst Complex.
Tay YD et al. Cell Rep 2019 Feb 05;26(6):1654-1667.e7
PMID:8675019 - Amiloride toxicity in the fission yeast Schizosaccharomyces pombe is released by thiamine and mutations in the thiamine-repressible gene car1.
Niederberger C et al. Gene 1996 May 24;171(1):119-22
PMID:26771498 - A Proteome-wide Fission Yeast Interactome Reveals Network Evolution Principles from Yeasts to Human.
Vo TV et al. Cell 2016 Jan 14;164(1-2):310-323