protein coding gene - rdp1 (SPAC6F12.09) - RNA-directed RNA polymerase (RDRC) Rdp1

Gene summary

Standard name

rdp1

Systematic ID

SPAC6F12.09

Product

RNA-directed RNA polymerase (RDRC) Rdp1

Organism

Schizosaccharomyces pombe (fission yeast)

Synonyms

rdr1, csp7

UniProt ID

O14227

ORFeome ID

39/39B11

Characterisation status

biological role published

Feature type

mRNA gene

Genomic location

chromosome I: 1324941..1329061 forward strand

Annotation

Protein features

Orthologs

• rdp1 (SJAG_03356) Schizosaccharomyces japonicus

References / Literature

PMID:37787768 - Broad functional profiling of fission yeast proteins using phenomics and machine learning.

Rodríguez-López M et al. Elife 2023 Oct 03;12

PMID:33823663 - A TOR (target of rapamycin) and nutritional phosphoproteome of fission yeast reveals novel targets in networks conserved in humans.

Halova L et al. Open Biol 2021 Apr;11(4):200405

PMID:22268381 - The karyopherin Sal3 is required for nuclear import of the core RNA interference pathway protein Rdp1.

Park J et al. Traffic 2012 Apr;13(4):520-31

PMID:28281664 - Genetic interactions and functional analyses of the fission yeast gsk3 and amk2 single and double mutants defective in TORC1-dependent processes.

Rallis C et al. Sci Rep 2017 Mar 10;7:44257

GO_REF:0000033 - Annotation inferences using phylogenetic trees

PMID:18345014 - Roles of the Clr4 methyltransferase complex in nucleation, spreading and maintenance of heterochromatin.

Zhang K et al. Nat Struct Mol Biol 2008 Apr;15(4):381-8

PMID:26771498 - A Proteome-wide Fission Yeast Interactome Reveals Network Evolution Principles from Yeasts to Human.

Vo TV et al. Cell 2016 Jan 14;164(1-2):310-323

PMID:21511999 - Comparative functional genomics of the fission yeasts.

Rhind N et al. Science 2011 May 20;332(6032):930-6

PMID:33378674 - The INO80 Complex Regulates Epigenetic Inheritance of Heterochromatin.

Shan CM et al. Cell Rep 2020 Dec 29;33(13):108561

PMID:37445861 - Resistance to Chemotherapeutic 5-Fluorouracil Conferred by Modulation of Heterochromatic Integrity through Ino80 Function in Fission Yeast.

Lim KK et al. Int J Mol Sci 2023 Jun 26;24(13)

PMID:14704433 - RNAi-mediated targeting of heterochromatin by the RITS complex.

Verdel A et al. Science 2004 Jan 30;303(5658):672-6

PMID:27538348 - Set3 contributes to heterochromatin integrity by promoting transcription of subunits of Clr4-Rik1-Cul4 histone methyltransferase complex in fission yeast.

Yu Y et al. Sci Rep 2016 Aug 19;6:31752

PMID:18684775 - A genome-wide screen of genes involved in cadmium tolerance in Schizosaccharomyces pombe.

Kennedy PJ et al. Toxicol Sci 2008 Nov;106(1):124-39

PMID:20211136 - Stc1: a critical link between RNAi and chromatin modification required for heterochromatin integrity.

Bayne EH et al. Cell 2010 Mar 05;140(5):666-77

PMID:41316862 - Deletion of Elongator Protein 1 (Elp1) relieves heterochromatin defects in a Pol II mutant of Schizosaccharomyces pombe.

Nirmal MB et al. Genetics 2025 Nov 29;

PMID:15632061 - Global effects on gene expression in fission yeast by silencing and RNA interference machineries.

Hansen KR et al. Mol Cell Biol 2005 Jan;25(2):590-601

PMID:39358553 - Ageing-associated long non-coding RNA extends lifespan and reduces translation in non-dividing cells.

Anver S et al. EMBO Rep 2024 Oct 02;

PMID:20178743 - Dicer-independent primal RNAs trigger RNAi and heterochromatin formation.

Halic M et al. Cell 2010 Feb 19;140(4):504-16

PMID:25274039 - A systematic genetic screen identifies new factors influencing centromeric heterochromatin integrity in fission yeast.

Bayne EH et al. Genome Biol 2014;15(10):481

PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.

Marguerat S et al. Cell 2012 Oct 26;151(3):671-83

PMID:22895252 - A novel RNAi protein, Dsh1, assembles RNAi machinery on chromatin to amplify heterochromatic siRNA.

Kawakami K et al. Genes Dev 2012 Aug 15;26(16):1811-24

PMID:22681890 - Hierarchical modularity and the evolution of genetic interactomes across species.

Ryan CJ et al. Mol Cell 2012 Jun 08;46(5):691-704

PMID:22431512 - RNAi keeps Atf1-bound stress response genes in check at nuclear pores.

Woolcock KJ et al. Genes Dev 2012 Apr 01;26(7):683-92

PMID:23151475 - RNAi triggered by specialized machinery silences developmental genes and retrotransposons.

Yamanaka S et al. Nature 2013 Jan 24;493(7433):557-60

PMID:20705239 - The methyltransferase activity of Clr4Suv39h triggers RNAi independently of histone H3K9 methylation.

Gerace EL et al. Mol Cell 2010 Aug 13;39(3):360-72

PMID:27738016 - RNA interference is essential for cellular quiescence.

Roche B et al. Science 2016 Nov 11;354(6313)

PMID:27984744 - Survival in Quiescence Requires the Euchromatic Deployment of Clr4/SUV39H by Argonaute-Associated Small RNAs.

Joh RI et al. Mol Cell 2016 Dec 15;64(6):1088-1101

PMID:40132111 - A fission yeast CENP-B homologue Abp1 prevents RNAi-mediated heterochromatin formation at ribosomal DNA repeats.

Tsunemine S et al. Genetics 2025 Mar 25;

PMID:23050226 - A genetic screen to discover pathways affecting cohesin function in Schizosaccharomyces pombe identifies chromatin effectors.

Chen Z et al. G3 (Bethesda) 2012 Oct;2(10):1161-8

PMID:28541282 - Tailing and degradation of Argonaute-bound small RNAs protect the genome from uncontrolled RNAi.

Pisacane P et al. Nat Commun 2017 May 25;8:15332

PMID:16797182 - Conserved ribonuclease, Eri1, negatively regulates heterochromatin assembly in fission yeast.

Iida T et al. Curr Biol 2006 Jul 25;16(14):1459-64

PMID:12193640 - Regulation of heterochromatic silencing and histone H3 lysine-9 methylation by RNAi.

Volpe TA et al. Science 2002 Sep 13;297(5588):1833-7

PMID:15475954 - RITS acts in cis to promote RNA interference-mediated transcriptional and post-transcriptional silencing.

Noma K et al. Nat Genet 2004 Nov;36(11):1174-80

PMID:29914874 - Long noncoding RNA repertoire and targeting by nuclear exosome, cytoplasmic exonuclease, and RNAi in fission yeast.

Atkinson SR et al. RNA 2018 Sep;24(9):1195-1213

PMID:19264558 - Screening a genome-wide S. pombe deletion library identifies novel genes and pathways involved in genome stability maintenance.

Deshpande GP et al. DNA Repair (Amst) 2009 May 01;8(5):672-9

PMID:19883398 - The Kinesin motor protein Cut7 regulates biogenesis and function of Ago1-complexes.

Stoica C et al. Traffic 2010 Jan;11(1):25-36

PMID:21151114 - Dicer associates with chromatin to repress genome activity in Schizosaccharomyces pombe.

Woolcock KJ et al. Nat Struct Mol Biol 2011 Jan;18(1):94-9

PMID:16738311 - Fission yeast Cid12 has dual functions in chromosome segregation and checkpoint control.

Win TZ et al. Mol Cell Biol 2006 Jun;26(12):4435-47

PMID:22733737 - Ers1 links HP1 to RNAi.

Rougemaille M et al. Proc Natl Acad Sci U S A 2012 Jul 10;109(28):11258-63

PMID:30652128 - Heterochromatin suppresses gross chromosomal rearrangements at centromeres by repressing Tfs1/TFIIS-dependent transcription.

Okita AK et al. Commun Biol 2019;2:17

PMID:32101745 - Dense Transposon Integration Reveals Essential Cleavage and Polyadenylation Factors Promote Heterochromatin Formation.

Lee SY et al. Cell Rep 2020 Feb 25;30(8):2686-2698.e8

PMID:34250083 - Barcode sequencing and a high-throughput assay for chronological lifespan uncover ageing-associated genes in fission yeast.

Romila CA et al. Microb Cell 2021 Jul 05;8(7):146-160

PMID:19111658 - HP1 proteins form distinct complexes and mediate heterochromatic gene silencing by nonoverlapping mechanisms.

Motamedi MR et al. Mol Cell 2008 Dec 26;32(6):778-90

PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.

Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623

PMID:26631744 - Conserved factor Dhp1/Rat1/Xrn2 triggers premature transcription termination and nucleates heterochromatin to promote gene silencing.

Chalamcharla VR et al. Proc Natl Acad Sci U S A 2015 Dec 22;112(51):15548-55

PMID:15218150 - RNAi-independent heterochromatin nucleation by the stress-activated ATF/CREB family proteins.

Jia S et al. Science 2004 Jun 25;304(5679):1971-6

PMID:15292231 - Regulation of Swi6/HP1-dependent heterochromatin assembly by cooperation of components of the mitogen-activated protein kinase pathway and a histone deacetylase Clr6.

Kim HS et al. J Biol Chem 2004 Oct 08;279(41):42850-9

PMID:24957674 - Yeast X-chromosome-associated protein 5 (Xap5) functions with H2A.Z to suppress aberrant transcripts.

Anver S et al. EMBO Rep 2014 Aug;15(8):894-902

PMID:24240238 - Elimination of shelterin components bypasses RNAi for pericentric heterochromatin assembly.

Tadeo X et al. Genes Dev 2013 Nov 15;27(22):2489-99

PMID:19164572 - Msc1 links dynamic Swi6/HP1 binding to cell fate determination.

Lawrence RJ et al. Proc Natl Acad Sci U S A 2009 Jan 27;106(4):1163-8

PMID:16823372 - ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe.

Matsuyama A et al. Nat Biotechnol 2006 Jul;24(7):841-7

PMID:25483073 - Chromosome segregation and organization are targets of 5'-Fluorouracil in eukaryotic cells.

Mojardín L et al. Cell Cycle 2015;14(2):206-18

PMID:21289066 - Elimination of a specific histone H3K14 acetyltransferase complex bypasses the RNAi pathway to regulate pericentric heterochromatin functions.

Reddy BD et al. Genes Dev 2011 Feb 01;25(3):214-9

PMID:15615848 - RNA-dependent RNA polymerase is an essential component of a self-enforcing loop coupling heterochromatin assembly to siRNA production.

Sugiyama T et al. Proc Natl Acad Sci U S A 2005 Jan 04;102(1):152-7

PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.

Hayles J et al. Open Biol 2013 May 22;3(5):130053

PMID:30726745 - Fission Yeast NDR/LATS Kinase Orb6 Regulates Exocytosis via Phosphorylation of the Exocyst Complex.

Tay YD et al. Cell Rep 2019 Feb 05;26(6):1654-1667.e7

PMID:18818364 - Conservation and rewiring of functional modules revealed by an epistasis map in fission yeast.

Roguev A et al. Science 2008 Oct 17;322(5900):405-10

PMID:24493644 - lncRNA recruits RNAi and the exosome to dynamically regulate pho1 expression in response to phosphate levels in fission yeast.

Shah S et al. Genes Dev 2014 Feb 01;28(3):231-44

PMID:22768388 - Translational control of cell division by Elongator.

Bauer F et al. Cell Rep 2012 May 31;1(5):424-33

PMID:28410370 - A systematic screen for morphological abnormalities during fission yeast sexual reproduction identifies a mechanism of actin aster formation for cell fusion.

Dudin O et al. PLoS Genet 2017 Apr;13(4):e1006721

PMID:20062003 - Hairpin RNA induces secondary small interfering RNA synthesis and silencing in trans in fission yeast.

Simmer F et al. EMBO Rep 2010 Feb;11(2):112-8

PMID:28357272 - A central role for TOR signalling in a yeast model for juvenile CLN3 disease.

Bond ME et al. Microb Cell 2015 Nov 11;2(12):466-480

PMID:22474355 - Heterochromatin protein 1 homologue Swi6 acts in concert with Ers1 to regulate RNAi-directed heterochromatin assembly.

Hayashi A et al. Proc Natl Acad Sci U S A 2012 Apr 17;109(16):6159-64

PMID:16931764 - Argonaute slicing is required for heterochromatic silencing and spreading.

Irvine DV et al. Science 2006 Aug 25;313(5790):1134-7

PMID:36617881 - Tandemly repeated genes promote RNAi-mediated heterochromatin formation via an antisilencing factor, Epe1, in fission yeast.

Asanuma T et al. Genes Dev 2022 Nov;36(21-24):1145-1159

PMID:28228545 - Ablation of RNA interference and retrotransposons accompany acquisition and evolution of transposases to heterochromatin protein CENPB.

Upadhyay U et al. Mol Biol Cell 2017 Apr 15;28(8):1132-1146

PMID:15607976 - Two RNAi complexes, RITS and RDRC, physically interact and localize to noncoding centromeric RNAs.

Motamedi MR et al. Cell 2004 Dec 17;119(6):789-802