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protein coding gene - cut23 (SPAC6F12.14) - anaphase-promoting complex TPR lobe subcomplex subunit Apc8

Gene summary

Standard name
cut23
Systematic ID
SPAC6F12.14
Product
anaphase-promoting complex TPR lobe subcomplex subunit Apc8
Organism
Schizosaccharomyces pombe (fission yeast)
Synonyms
apc8
UniProt ID
O94556
ORFeome ID
27/27E10
Characterisation status
biological role published
Feature type
mRNA gene
Genomic location
chromosome I: 1340221..1342448 forward strand

Annotation

GO biological process

GO:0031145 - anaphase-promoting complex-dependent catabolic process

References:

GO:1901970 - positive regulation of mitotic sister chromatid separation

References:

GO cellular component

GO:0005680 - anaphase-promoting complex

References:

GO:0044732 - mitotic spindle pole body

References:

GO:0005634 - nucleus

References:

GO molecular function

GO:0140767 - enzyme-substrate adaptor activity

References:

GO:0005515 - protein binding

References:

Modification

MOD:00046 - O-phospho-L-serine

References:

MOD:00696 - phosphorylated residue

References:

MOD:01148 - ubiquitinylated lysine

References:

Multi-locus phenotype

FYPO:0005055 - binucleate multiseptate cell, septa grouped

References:

Genotypes:

FYPO:0000082 - decreased cell population growth at high temperature

References:

Genotypes:

FYPO:0000411 - normal mitotic cell cycle

References:

Genotypes:

FYPO:0000673 - normal septum assembly

References:

Genotypes:

FYPO:0005440 - swollen elongated cell with enlarged nucleus

References:

Genotypes:

Protein features

PBO:0111755 - TPR repeat protein

Qualitative gene expression

PomGeneEx:0000018 - protein level increased

References:

PomGeneEx:0000011 - RNA level increased

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Quantitative gene expression

PBO:0006310 - protein level

References:

PBO:0011963 - RNA level

References:

Single locus phenotype

FYPO:0000620 - abnormal cell cycle arrest in mitotic metaphase

References:

Genotypes:

FYPO:0003593 - abolished anaphase-promoting complex-dependent protein catabolic process

References:

Genotypes:

FYPO:0001946 - abolished mitotic sister chromatid separation

References:

Genotypes:

FYPO:0000705 - abolished protein-protein interaction

References:

Genotypes:

FYPO:0001055 - cut following normal mitotic chromosome condensation

References:

Genotypes:

FYPO:0001645 - decreased protein-protein interaction

References:

Genotypes:

FYPO:0000310 - inviable after spore germination, without cell division, with normal germ tube morphology

References:

Genotypes:

FYPO:0005321 - inviable elongated mononucleate aseptate cell with cell cycle arrest in mitotic metaphase

References:

Genotypes:

FYPO:0001490 - inviable elongated vegetative cell

References:

Genotypes:

FYPO:0002061 - inviable vegetative cell population

References:

Genotypes:

FYPO:0002273 - inviable vegetative cell with abnormal cell morphology

References:

Genotypes:

FYPO:0003166 - monoseptate vegetative cell with binucleate and anucleate compartments

References:

Genotypes:

FYPO:0005322 - normal anaphase-promoting complex assembly

References:

Genotypes:

FYPO:0000280 - sterile

References:

Genotypes:

Taxonomic conservation

PBO:0011065 - conserved in eukaryotes

PBO:0011071 - conserved in eukaryotes only

PBO:0011064 - conserved in fungi

PBO:0011069 - conserved in metazoa

PBO:0011070 - conserved in vertebrates

PBO:0006222 - predominantly single copy (one to one)

Protein features

IDNameInterPro nameDB name
PF04049ANAPC8APC8Pfam
PF13176TPR_7TPR_rptPfam
PF13181TPR_8TPR_rptPfam
PS50005TPRTPR_rptPROSITE profiles
SM00028TPRTPR_rptSMART
G3DSA:1.25.40.10:FF:003705CATH-FunFam
G3DSA:1.25.40.10TPR-like_helical_dom_sfCATH-Gene3D
SSF48452TPR-like_helical_dom_sfSUPERFAMILY
PTHR12558PANTHER

Orthologs

References / Literature

PMID:26771498 - A Proteome-wide Fission Yeast Interactome Reveals Network Evolution Principles from Yeasts to Human.
Vo TV et al. Cell 2016 Jan 14;164(1-2):310-323
PMID:24713849 - Post-transcriptional regulation of meiotic genes by a nuclear RNA silencing complex.
Egan ED et al. RNA 2014 Jun;20(6):867-81
PMID:24763107 - Absolute proteome and phosphoproteome dynamics during the cell cycle of Schizosaccharomyces pombe (Fission Yeast).
Carpy A et al. Mol Cell Proteomics 2014 Aug;13(8):1925-36
PMID:12477395 - Proteomics analysis identifies new components of the fission and budding yeast anaphase-promoting complexes.
Yoon HJ et al. Curr Biol 2002 Dec 10;12(23):2048-54
PMID:16823372 - ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe.
Matsuyama A et al. Nat Biotechnol 2006 Jul;24(7):841-7
PMID:15507118 - An interactive gene network for securin-separase, condensin, cohesin, Dis1/Mtc1 and histones constructed by mass transformation.
Yuasa T et al. Genes Cells 2004 Nov;9(11):1069-82
PMID:37970674 - SUMOylation regulates Lem2 function in centromere clustering and silencing.
Strachan J et al. J Cell Sci 2023 Dec 01;136(23)
PMID:15060174 - Swm1/Apc13 is an evolutionarily conserved subunit of the anaphase-promoting complex stabilizing the association of Cdc16 and Cdc27.
Schwickart M et al. Mol Cell Biol 2004 Apr;24(8):3562-76
PMID:36108046 - Recovery from spindle checkpoint-mediated arrest requires a novel Dnt1-dependent APC/C activation mechanism.
Bai S et al. PLoS Genet 2022 Sep;18(9):e1010397
PMID:39367033 - Quantitative proteomics and phosphoproteomics profiling of meiotic divisions in the fission yeast Schizosaccharomyces pombe.
Sivakova B et al. Sci Rep 2024 Oct 04;14(1):23105
PMID:10582241 - Control of metaphase-anaphase progression by proteolysis: cyclosome function regulated by the protein kinase A pathway, ubiquitination and localization.
Yanagida M et al. Philos Trans R Soc Lond B Biol Sci 1999 Sep 29;354(1389):1559-69; discussion 1569-70
PMID:23442800 - An E2 enzyme Ubc11 is required for ubiquitination of Slp1/Cdc20 and spindle checkpoint silencing in fission yeast.
Horikoshi Y et al. Cell Cycle 2013 Mar 15;12(6):961-71
PMID:26960127 - Data collection with a tailored X-ray beam size at 2.69 Å wavelength (4.6 keV): sulfur SAD phasing of Cdc23(Nterm).
Cianci M et al. Acta Crystallogr D Struct Biol 2016 Mar;72(Pt 3):403-12
PMID:10526233 - Fission yeast APC/cyclosome subunits, Cut20/Apc4 and Cut23/Apc8, in regulating metaphase-anaphase progression and cellular stress responses.
Yamashita YM et al. Genes Cells 1999 Aug;4(8):445-63
PMID:18556659 - The spindle checkpoint functions of Mad3 and Mad2 depend on a Mad3 KEN box-mediated interaction with Cdc20-anaphase-promoting complex (APC/C).
Sczaniecka M et al. J Biol Chem 2008 Aug 22;283(34):23039-47
PMID:21511999 - Comparative functional genomics of the fission yeasts.
Rhind N et al. Science 2011 May 20;332(6032):930-6
PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.
Hayles J et al. Open Biol 2013 May 22;3(5):130053
PMID:11777938 - Polo boxes and Cut23 (Apc8) mediate an interaction between polo kinase and the anaphase-promoting complex for fission yeast mitosis.
May KM et al. J Cell Biol 2002 Jan 07;156(1):23-8
GO_REF:0000033 - Annotation inferences using phylogenetic trees
PMID:18082611 - Structural organization of the anaphase-promoting complex bound to the mitotic activator Slp1.
Ohi MD et al. Mol Cell 2007 Dec 14;28(5):871-85
PMID:16950791 - Role of Hcn1 and its phosphorylation in fission yeast anaphase-promoting complex/cyclosome function.
Yoon HJ et al. J Biol Chem 2006 Oct 27;281(43):32284-93
PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.
Marguerat S et al. Cell 2012 Oct 26;151(3):671-83
PMID:12653962 - A brute force postgenome approach to identify temperature-sensitive mutations that negatively interact with separase and securin plasmids.
Matsumura T et al. Genes Cells 2003 Apr;8(4):341-55
PMID:27298342 - Identification of S-phase DNA damage-response targets in fission yeast reveals conservation of damage-response networks.
Willis NA et al. Proc Natl Acad Sci U S A 2016 Jun 28;113(26):E3676-85
PMID:12615979 - Polo boxes form a single functional domain that mediates interactions with multiple proteins in fission yeast polo kinase.
Reynolds N et al. J Cell Sci 2003 Apr 01;116(Pt 7):1377-87
PMID:12161753 - The transcriptional program of meiosis and sporulation in fission yeast.
Mata J et al. Nat Genet 2002 Sep;32(1):143-7
PMID:18354085 - Diminishing HDACs by drugs or mutations promotes normal or abnormal sister chromatid separation by affecting APC/C and adherin.
Kimata Y et al. J Cell Sci 2008 Apr 01;121(Pt 7):1107-18
PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.
Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623
PMID:23583778 - The four canonical tpr subunits of human APC/C form related homo-dimeric structures and stack in parallel to form a TPR suprahelix.
Zhang Z et al. J Mol Biol 2013 Nov 15;425(22):4236-48
PMID:30355493 - Expanded Interactome of the Intrinsically Disordered Protein Dss1.
Schenstrøm SM et al. Cell Rep 2018 Oct 23;25(4):862-870