protein coding gene - cut9 (SPAC6F12.15c) - anaphase-promoting complex TPR lobe subcomplex subunit Cut9/Apc6

Gene summary

Standard name

cut9

Systematic ID

SPAC6F12.15c

Product

anaphase-promoting complex TPR lobe subcomplex subunit Cut9/Apc6

Organism

Schizosaccharomyces pombe (fission yeast)

Synonyms

dre1, apc6

UniProt ID

P41889

ORFeome ID

37/37D09

Characterisation status

biological role published

Feature type

mRNA gene

Genomic location

chromosome I: 1342057..1344785 reverse strand

Annotation

Protein features

Orthologs

• CDC16 (HGNC:1720) Homo sapiens
• cut9 (SJAG_03350) Schizosaccharomyces japonicus
• CDC16 (YKL022C) Saccharomyces cerevisiae

References / Literature

PMID:31719112 - Checkpoint Regulation of Nuclear Tos4 Defines S Phase Arrest in Fission Yeast.

Kim SM et al. G3 (Bethesda) 2020 Jan 07;10(1):255-266

PMID:10921876 - APC(ste9/srw1) promotes degradation of mitotic cyclins in G(1) and is inhibited by cdc2 phosphorylation.

Blanco MA et al. EMBO J 2000 Aug 01;19(15):3945-55

PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.

Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623

PMID:35320724 - Cdc48 influence on separase levels is independent of mitosis and suggests translational sensitivity of separase.

Vijayakumari D et al. Cell Rep 2022 Mar 22;38(12):110554

PMID:18556659 - The spindle checkpoint functions of Mad3 and Mad2 depend on a Mad3 KEN box-mediated interaction with Cdc20-anaphase-promoting complex (APC/C).

Sczaniecka M et al. J Biol Chem 2008 Aug 22;283(34):23039-47

PMID:24161933 - Determinants of robustness in spindle assembly checkpoint signalling.

Heinrich S et al. Nat Cell Biol 2013 Nov;15(11):1328-39

PMID:28366744 - Fission Yeast Apc15 Stabilizes MCC-Cdc20-APC/C Complexes, Ensuring Efficient Cdc20 Ubiquitination and Checkpoint Arrest.

May KM et al. Curr Biol 2017 Apr 24;27(8):1221-1228

PMID:16682348 - A screen for cohesion mutants uncovers Ssl3, the fission yeast counterpart of the cohesin loading factor Scc4.

Bernard P et al. Curr Biol 2006 May 09;16(9):875-81

PMID:18082611 - Structural organization of the anaphase-promoting complex bound to the mitotic activator Slp1.

Ohi MD et al. Mol Cell 2007 Dec 14;28(5):871-85

PMID:26527280 - Robust Ordering of Anaphase Events by Adaptive Thresholds and Competing Degradation Pathways.

Kamenz J et al. Mol Cell 2015 Nov 05;60(3):446-59

PMID:20924356 - The APC/C subunit Cdc16/Cut9 is a contiguous tetratricopeptide repeat superhelix with a homo-dimer interface similar to Cdc27.

Zhang Z et al. EMBO J 2010 Nov 03;29(21):3733-44

PMID:24713849 - Post-transcriptional regulation of meiotic genes by a nuclear RNA silencing complex.

Egan ED et al. RNA 2014 Jun;20(6):867-81

PMID:15507118 - An interactive gene network for securin-separase, condensin, cohesin, Dis1/Mtc1 and histones constructed by mass transformation.

Yuasa T et al. Genes Cells 2004 Nov;9(11):1069-82

PMID:7798319 - Bypassing anaphase by fission yeast cut9 mutation: requirement of cut9+ to initiate anaphase.

Samejima I et al. J Cell Biol 1994 Dec;127(6 Pt 1):1655-70

PMID:9808627 - Faithful anaphase is ensured by Mis4, a sister chromatid cohesion molecule required in S phase and not destroyed in G1 phase.

Furuya K et al. Genes Dev 1998 Nov 01;12(21):3408-18

PMID:9264466 - Distinct subunit functions and cell cycle regulated phosphorylation of 20S APC/cyclosome required for anaphase in fission yeast.

Yamada H et al. J Cell Sci 1997 Aug;110 ( Pt 15):1793-804

GO_REF:0000033 - Annotation inferences using phylogenetic trees

PMID:18354085 - Diminishing HDACs by drugs or mutations promotes normal or abnormal sister chromatid separation by affecting APC/C and adherin.

Kimata Y et al. J Cell Sci 2008 Apr 01;121(Pt 7):1107-18

PMID:11279037 - Two WD repeat-containing TATA-binding protein-associated factors in fission yeast that suppress defects in the anaphase-promoting complex.

Mitsuzawa H et al. J Biol Chem 2001 May 18;276(20):17117-24

PMID:16950791 - Role of Hcn1 and its phosphorylation in fission yeast anaphase-promoting complex/cyclosome function.

Yoon HJ et al. J Biol Chem 2006 Oct 27;281(43):32284-93

PMID:15161942 - The nucleolus is involved in mRNA export from the nucleus in fission yeast.

Ideue T et al. J Cell Sci 2004 Jun 15;117(Pt 14):2887-95

PMID:8918880 - 20S cyclosome complex formation and proteolytic activity inhibited by the cAMP/PKA pathway.

Yamashita YM et al. Nature 1996 Nov 21;384(6606):276-9

PMID:12653962 - A brute force postgenome approach to identify temperature-sensitive mutations that negatively interact with separase and securin plasmids.

Matsumura T et al. Genes Cells 2003 Apr;8(4):341-55

PMID:30355493 - Expanded Interactome of the Intrinsically Disordered Protein Dss1.

Schenstrøm SM et al. Cell Rep 2018 Oct 23;25(4):862-870

PMID:10526233 - Fission yeast APC/cyclosome subunits, Cut20/Apc4 and Cut23/Apc8, in regulating metaphase-anaphase progression and cellular stress responses.

Yamashita YM et al. Genes Cells 1999 Aug;4(8):445-63

PMID:25720772 - Quantitative phosphoproteomics reveals pathways for coordination of cell growth and division by the conserved fission yeast kinase pom1.

Kettenbach AN et al. Mol Cell Proteomics 2015 May;14(5):1275-87

PMID:21511999 - Comparative functional genomics of the fission yeasts.

Rhind N et al. Science 2011 May 20;332(6032):930-6

PMID:26771498 - A Proteome-wide Fission Yeast Interactome Reveals Network Evolution Principles from Yeasts to Human.

Vo TV et al. Cell 2016 Jan 14;164(1-2):310-323

PMID:37970674 - SUMOylation regulates Lem2 function in centromere clustering and silencing.

Strachan J et al. J Cell Sci 2023 Dec 01;136(23)

PMID:12477395 - Proteomics analysis identifies new components of the fission and budding yeast anaphase-promoting complexes.

Yoon HJ et al. Curr Biol 2002 Dec 10;12(23):2048-54

PMID:18414064 - Schizosaccharomyces pombe Orc5 plays multiple roles in the maintenance of genome stability throughout the cell cycle.

Kato H et al. Cell Cycle 2008 Apr 15;7(8):1085-96

PMID:23986474 - Mutation of a conserved residue enhances the sensitivity of analogue-sensitised kinases to generate a novel approach to the study of mitosis in fission yeast.

Tay YD et al. J Cell Sci 2013 Nov 01;126(Pt 21):5052-61

PMID:9475720 - Isolation of a Schizosaccharomyces pombe rad21ts mutant that is aberrant in chromosome segregation, microtubule function, DNA repair and sensitive to hydroxyurea: possible involvement of Rad21 in ubiquitin-mediated proteolysis.

Tatebayashi K et al. Genetics 1998 Jan;148(1):49-57

PMID:2203537 - The fission yeast cut1+ gene regulates spindle pole body duplication and has homology to the budding yeast ESP1 gene.

Uzawa S et al. Cell 1990 Sep 07;62(5):913-25

PMID:8632802 - Cut2 proteolysis required for sister-chromatid seperation in fission yeast.

Funabiki H et al. Nature 1996 May 30;381(6581):438-41

PMID:39367033 - Quantitative proteomics and phosphoproteomics profiling of meiotic divisions in the fission yeast Schizosaccharomyces pombe.

Sivakova B et al. Sci Rep 2024 Oct 04;14(1):23105

PMID:33313903 - Ribosome profiling reveals ribosome stalling on tryptophan codons and ribosome queuing upon oxidative stress in fission yeast.

Rubio A et al. Nucleic Acids Res 2021 Jan 11;49(1):383-399

PMID:35970865 - The Mis6 inner kinetochore subcomplex maintains CENP-A nucleosomes against centromeric non-coding transcription during mitosis.

Hirai H et al. Commun Biol 2022 Aug 15;5(1):818

PMID:28218250 - Chromatin remodeller Fun30 Fft3 induces nucleosome disassembly to facilitate RNA polymerase II elongation.

Lee J et al. Nat Commun 2017 Feb 20;8:14527

PMID:39476757 - Characterization of Ksg1 protein kinase-dependent phosphoproteome in the fission yeast S. pombe.

Cipak L et al. Biochem Biophys Res Commun 2024 Oct 25;736:150895

PMID:11058086 - High dosage expression of a zinc finger protein, Grt1, suppresses a mutant of fission yeast slp1(+), a homolog of CDC20/p55CDC/Fizzy.

Yamada HY et al. J Cell Sci 2000 Nov;113 ( Pt 22):3989-99

PMID:15466421 - A screen for Schizosaccharomyces pombe mutants defective in rereplication identifies new alleles of rad4+, cut9+ and psf2+.

Gómez EB et al. Genetics 2005 Jan;169(1):77-89

PMID:8978689 - Requirement for PP1 phosphatase and 20S cyclosome/APC for the onset of anaphase is lessened by the dosage increase of a novel gene sds23+.

Ishii K et al. EMBO J 1996 Dec 02;15(23):6629-40

PMID:9461438 - Fission yeast Slp1: an effector of the Mad2-dependent spindle checkpoint.

Kim SH et al. Science 1998 Feb 13;279(5353):1045-7

PMID:15126629 - Suppression of a mitotic mutant by tRNA-Ala anticodon mutations that produce a dominant defect in late mitosis.

Kimata Y et al. J Cell Sci 2004 May 01;117(Pt 11):2283-93

PMID:15060174 - Swm1/Apc13 is an evolutionarily conserved subunit of the anaphase-promoting complex stabilizing the association of Cdc16 and Cdc27.

Schwickart M et al. Mol Cell Biol 2004 Apr;24(8):3562-76

PMID:10082519 - The schizosaccharomyces pombe dim1(+) gene interacts with the anaphase-promoting complex or cyclosome (APC/C) component lid1(+) and is required for APC/C function.

Berry LD et al. Mol Cell Biol 1999 Apr;19(4):2535-46

PMID:16453724 - Isolation and characterization of Schizosaccharomyces pombe cutmutants that block nuclear division but not cytokinesis.

Hirano T et al. EMBO J 1986 Nov;5(11):2973-9

PMID:11084332 - Cut8, essential for anaphase, controls localization of 26S proteasome, facilitating destruction of cyclin and Cut2.

Tatebe H et al. Curr Biol 2000 Nov 02;10(21):1329-38

PMID:37694715 - A ubiquitin-proteasome pathway degrades the inner nuclear membrane protein Bqt4 to maintain nuclear membrane homeostasis.

Le TK et al. J Cell Sci 2023 Oct 01;136(19)

PMID:9154838 - A ubiquitin-conjugating enzyme in fission yeast that is essential for the onset of anaphase in mitosis.

Osaka F et al. Mol Cell Biol 1997 Jun;17(6):3388-97

PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.

Hayles J et al. Open Biol 2013 May 22;3(5):130053

PMID:9223296 - The Schizosaccharomyces pombe spindle checkpoint protein mad2p blocks anaphase and genetically interacts with the anaphase-promoting complex.

He X et al. Proc Natl Acad Sci U S A 1997 Jul 22;94(15):7965-70

PMID:24763107 - Absolute proteome and phosphoproteome dynamics during the cell cycle of Schizosaccharomyces pombe (Fission Yeast).

Carpy A et al. Mol Cell Proteomics 2014 Aug;13(8):1925-36

PMID:10582241 - Control of metaphase-anaphase progression by proteolysis: cyclosome function regulated by the protein kinase A pathway, ubiquitination and localization.

Yanagida M et al. Philos Trans R Soc Lond B Biol Sci 1999 Sep 29;354(1389):1559-69; discussion 1569-70

PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.

Marguerat S et al. Cell 2012 Oct 26;151(3):671-83

PMID:16823372 - ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe.

Matsuyama A et al. Nat Biotechnol 2006 Jul;24(7):841-7

PMID:30647105 - Comparative Genomic Screen in Two Yeasts Reveals Conserved Pathways in the Response Network to Phenol Stress.

Alhoch B et al. G3 (Bethesda) 2019 Mar 07;9(3):639-650

PMID:33506191 - Escape from mitotic catastrophe by actin-dependent nuclear displacement in fission yeast.

Yukawa M et al. iScience 2021 Jan 22;24(1):102031

PMID:9001228 - A fission yeast homolog of CDC20/p55CDC/Fizzy is required for recovery from DNA damage and genetically interacts with p34cdc2.

Matsumoto T Mol Cell Biol 1997 Feb;17(2):742-50

PMID:12615927 - Interaction of the anaphase-promoting complex/cyclosome and proteasome protein complexes with multiubiquitin chain-binding proteins.

Seeger M et al. J Biol Chem 2003 May 09;278(19):16791-6

PMID:8395535 - Identification of seven new cut genes involved in Schizosaccharomyces pombe mitosis.

Samejima I et al. J Cell Sci 1993 May;105 ( Pt 1):135-43

PMID:19117951 - Interaction of APC/C-E3 ligase with Swi6/HP1 and Clr4/Suv39 in heterochromatin assembly in fission yeast.

Dubey RN et al. J Biol Chem 2009 Mar 13;284(11):7165-76

PMID:12242294 - The mal2p protein is an essential component of the fission yeast centromere.

Jin QW et al. Mol Cell Biol 2002 Oct;22(20):7168-83

PMID:24948786 - Histone H2B ubiquitination promotes the function of the anaphase-promoting complex/cyclosome in Schizosaccharomyces pombe.

Elmore ZC et al. G3 (Bethesda) 2014 Jun 19;4(8):1529-38

PMID:38780300 - Nitrogen availability is important for preventing catastrophic mitosis in fission yeast.

Zemlianski V et al. J Cell Sci 2024 May 23;

PMID:28366743 - Different Functionality of Cdc20 Binding Sites within the Mitotic Checkpoint Complex.

Sewart K et al. Curr Biol 2017 Apr 24;27(8):1213-1220

PMID:12724408 - Two ubiquitin-conjugating enzymes, UbcP1/Ubc4 and UbcP4/Ubc11, have distinct functions for ubiquitination of mitotic cyclin.

Seino H et al. Mol Cell Biol 2003 May;23(10):3497-505

PMID:9755167 - The role of the destruction box and its neighbouring lysine residues in cyclin B for anaphase ubiquitin-dependent proteolysis in fission yeast: defining the D-box receptor.

Yamano H et al. EMBO J 1998 Oct 01;17(19):5670-8