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protein coding gene - rna14 (SPAC6F12.17) - mRNA cleavage and polyadenylation specificity factor complex subunit Rna14

Gene summary

Standard name
rna14
Systematic ID
SPAC6F12.17
Product
mRNA cleavage and polyadenylation specificity factor complex subunit Rna14
Organism
Schizosaccharomyces pombe (fission yeast)
UniProt ID
O14233
ORFeome ID
28/28A11
Characterisation status
biological role published
Feature type
mRNA gene
Genomic location
chromosome I: 1348764..1351231 forward strand

Annotation

GO biological process

GO:0180010 - co-transcriptional mRNA 3'-end processing, cleavage and polyadenylation pathway

References:

GO cellular component

GO:0000785 - chromatin

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GO:0005848 - mRNA cleavage stimulating factor complex

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GO:0005634 - nucleus

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GO molecular function

GO:0003729 - mRNA binding

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Modification

MOD:00046 - O-phospho-L-serine

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MOD:00696 - phosphorylated residue

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MOD:01149 - sumoylated lysine

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Multi-locus phenotype

FYPO:0001407 - decreased cell population growth on glucose carbon source

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Genotypes:

FYPO:0002303 - inviable mononucleate monoseptate vegetative cell with anucleate compartment

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Genotypes:

FYPO:0000838 - normal protein localization to nucleus during vegetative growth

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Genotypes:

FYPO:0000091 - sensitive to thiabendazole

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Genotypes:

Protein features

PBO:0111822 - HAT repeat protein

Quantitative gene expression

PBO:0006310 - protein level

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PBO:0011963 - RNA level

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Single locus phenotype

FYPO:0000359 - abnormal mitochondrial morphology

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Genotypes:

FYPO:0000141 - abnormal mitotic sister chromatid segregation

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Genotypes:

FYPO:0006667 - abnormal nuclear morphology during G0

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Genotypes:

FYPO:0004811 - abnormal termination of RNA polymerase II transcription

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Genotypes:

FYPO:0004481 - abolished cell population growth at high temperature

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Genotypes:

FYPO:0003602 - abolished mRNA splicing, via spliceosome

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Genotypes:

FYPO:0000227 - chromosome loss during mitotic chromosome segregation

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Genotypes:

FYPO:0000229 - cut

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Genotypes:

FYPO:0001052 - cut, small cell

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Genotypes:

FYPO:0001407 - decreased cell population growth on glucose carbon source

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Genotypes:

FYPO:0003412 - decreased chromatin silencing at centromere outer repeat

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Genotypes:

FYPO:0003029 - decreased mRNA splicing, via spliceosome

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Genotypes:

FYPO:0001324 - decreased protein level during vegetative growth

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Genotypes:

FYPO:0006613 - decreased termination of RNA polymerase II transcription

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Genotypes:

FYPO:0001355 - decreased vegetative cell population growth

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Genotypes:

FYPO:0000639 - delayed onset of septum assembly

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Genotypes:

FYPO:0000614 - increased duration of mitotic S phase

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Genotypes:

FYPO:0001840 - increased minichromosome loss during vegetative growth

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Genotypes:

FYPO:0005116 - increased punctate nuclear protein localization

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Genotypes:

FYPO:0003049 - increased transcriptional readthrough

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Genotypes:

FYPO:0000311 - inviable after spore germination with normal, unseptated germ tube morphology

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Genotypes:

FYPO:0002059 - inviable cell population

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Genotypes:

FYPO:0002303 - inviable mononucleate monoseptate vegetative cell with anucleate compartment

References:

Genotypes:

FYPO:0002151 - inviable spore

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Genotypes:

FYPO:0002061 - inviable vegetative cell population

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Genotypes:

FYPO:0001387 - loss of viability at high temperature

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Genotypes:

FYPO:0006660 - loss of viability upon G0 to G1 transition

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Genotypes:

FYPO:0001164 - normal growth on glucose carbon source

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Genotypes:

FYPO:0001357 - normal vegetative cell population growth

References:

Genotypes:

FYPO:0000091 - sensitive to thiabendazole

References:

Genotypes:

Taxonomic conservation

PBO:0011065 - conserved in eukaryotes

PBO:0011071 - conserved in eukaryotes only

PBO:0011064 - conserved in fungi

PBO:0011069 - conserved in metazoa

PBO:0011070 - conserved in vertebrates

PBO:0006222 - predominantly single copy (one to one)

Protein features

IDNameInterPro nameDB name
PF05843SufSufPFAM
SM00386hat_new_1HATSMART
SSF48452TPR-likeTPR-like_helical_dom_sfSUPERFAMILY
G3DSA:1.25.40.1040GENE3D
PTHR19980RNA CLEAVAGE STIMULATION FACTORRna14-likePANTHER
mobidb-lite-Disorderdisorder_predictionMOBIDB-Disorder
mobidb-lite-Low-complexitydisorder_predictionMOBIDB-Low-complexity
mobidb-lite-Polardisorder_predictionMOBIDB-Polar

Orthologs

References / Literature

PMID:30726745 - Fission Yeast NDR/LATS Kinase Orb6 Regulates Exocytosis via Phosphorylation of the Exocyst Complex.
Tay YD et al. Cell Rep 2019 Feb 05;26(6):1654-1667.e7
PMID:32101745 - Dense Transposon Integration Reveals Essential Cleavage and Polyadenylation Factors Promote Heterochromatin Formation.
Lee SY et al. Cell Rep 2020 Feb 25;30(8):2686-2698.e8
PMID:31269446 - CPF Recruitment to Non-canonical Transcription Termination Sites Triggers Heterochromatin Assembly and Gene Silencing.
Vo TV et al. Cell Rep 2019 Jul 02;28(1):267-281.e5
PMID:30076928 - Point mutation A394E in the central intrinsic disordered region of Rna14 leads to chromosomal instability in fission yeast.
Sonkar A et al. Int J Biol Macromol 2018 Nov;119:785-791
PMID:24763107 - Absolute proteome and phosphoproteome dynamics during the cell cycle of Schizosaccharomyces pombe (Fission Yeast).
Carpy A et al. Mol Cell Proteomics 2014 Aug;13(8):1925-36
PMID:29996109 - Quantitative Phosphoproteomics Reveals the Signaling Dynamics of Cell-Cycle Kinases in the Fission Yeast Schizosaccharomyces pombe.
Swaffer MP et al. Cell Rep 2018 Jul 10;24(2):503-514
PMID:26771498 - A Proteome-wide Fission Yeast Interactome Reveals Network Evolution Principles from Yeasts to Human.
Vo TV et al. Cell 2016 Jan 14;164(1-2):310-323
PMID:21712547 - Mitotic substrates of the kinase aurora with roles in chromatin regulation identified through quantitative phosphoproteomics of fission yeast.
Koch A et al. Sci Signal 2011 Jun 28;4(179):rs6
PMID:33823662 - Multiple nutritional phenotypes of fission yeast mutants defective in genes encoding essential mitochondrial proteins.
Uehara L et al. Open Biol 2021 Apr;11(4):200369
PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.
Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623
PMID:24713849 - Post-transcriptional regulation of meiotic genes by a nuclear RNA silencing complex.
Egan ED et al. RNA 2014 Jun;20(6):867-81
GO_REF:0000033 - Annotation inferences using phylogenetic trees
GO_REF:0000024 - Manual transfer of experimentally-verified manual GO annotation data to orthologs by curator judgment of sequence similarity.
PMID:27401558 - The Nrd1-like protein Seb1 coordinates cotranscriptional 3' end processing and polyadenylation site selection.
Lemay JF et al. Genes Dev 2016 Jul 01;30(13):1558-72
PMID:28367989 - The conserved protein Seb1 drives transcription termination by binding RNA polymerase II and nascent RNA.
Wittmann S et al. Nat Commun 2017 Apr 03;8:14861
PMID:39476757 - Characterization of Ksg1 protein kinase-dependent phosphoproteome in the fission yeast S. pombe.
Cipak L et al. Biochem Biophys Res Commun 2024 Oct 25;736:150895
PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.
Hayles J et al. Open Biol 2013 May 22;3(5):130053
PMID:32499400 - Nutrient-dependent control of RNA polymerase II elongation rate regulates specific gene expression programs by alternative polyadenylation.
Yague-Sanz C et al. Genes Dev 2020 Jul 01;34(13-14):883-897
PMID:31615333 - Condensin locates at transcriptional termination sites in mitosis, possibly releasing mitotic transcripts.
Nakazawa N et al. Open Biol 2019 Oct 31;9(10):190125
PMID:39367033 - Quantitative proteomics and phosphoproteomics profiling of meiotic divisions in the fission yeast Schizosaccharomyces pombe.
Sivakova B et al. Sci Rep 2024 Oct 04;14(1):23105
PMID:27350684 - Mutant allele of rna14 in fission yeast affects pre-mRNA splicing.
Yadav S et al. J Genet 2016 Jun;95(2):389-97
PMID:21511999 - Comparative functional genomics of the fission yeasts.
Rhind N et al. Science 2011 May 20;332(6032):930-6
PMID:26581324 - Cleavage and polyadenylation factor, Rna14 is an essential protein required for the maintenance of genomic integrity in fission yeast Schizosaccharomyces pombe.
Sonkar A et al. Biochim Biophys Acta 2016 Feb;1863(2):189-97
PMID:33823663 - A TOR (target of rapamycin) and nutritional phosphoproteome of fission yeast reveals novel targets in networks conserved in humans.
Halova L et al. Open Biol 2021 Apr;11(4):200405
PMID:26537787 - Targeting of SUMO substrates to a Cdc48-Ufd1-Npl4 segregase and STUbL pathway in fission yeast.
Køhler JB et al. Nat Commun 2015 Nov 05;6:8827
PMID:19547744 - Evolution of phosphoregulation: comparison of phosphorylation patterns across yeast species.
Beltrao P et al. PLoS Biol 2009 Jun 16;7(6):e1000134
PMID:16823372 - ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe.
Matsuyama A et al. Nat Biotechnol 2006 Jul;24(7):841-7
PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.
Marguerat S et al. Cell 2012 Oct 26;151(3):671-83
PMID:28567704 - Fission yeast Ctf1, a cleavage and polyadenylation factor subunit is required for the maintenance of genomic integrity.
Sonkar A et al. Mol Genet Genomics 2017 Oct;292(5):1027-1036