protein coding gene - cdc16 (SPAC6F6.08c) - SIN signalling inhibitor, two-component GAP Cdc16

Gene summary

Standard name

cdc16

Systematic ID

SPAC6F6.08c

Product

SIN signalling inhibitor, two-component GAP Cdc16

Organism

Schizosaccharomyces pombe (fission yeast)

Synonyms

bub2

UniProt ID

P36618

ORFeome ID

44/44A11

Characterisation status

biological role published

Feature type

mRNA gene

Genomic location

chromosome I: 2746667..2748156 reverse strand

Annotation

Protein features

Orthologs

• cdc16 (SJAG_01386) Schizosaccharomyces japonicus
• BUB2 (YMR055C) Saccharomyces cerevisiae

References / Literature

PMID:11683392 - Flp1, a fission yeast orthologue of the s. cerevisiae CDC14 gene, is not required for cyclin degradation or rum1p stabilisation at the end of mitosis.

Cueille N et al. J Cell Sci 2001 Jul;114(Pt 14):2649-64

PMID:15121844 - The splicing factor U2AF small subunit is functionally conserved between fission yeast and humans.

Webb CJ et al. Mol Cell Biol 2004 May;24(10):4229-40

PMID:21131906 - Dma1 ubiquitinates the SIN scaffold, Sid4, to impede the mitotic localization of Plo1 kinase.

Johnson AE et al. EMBO J 2011 Jan 19;30(2):341-54

PMID:20498706 - Tip1/CLIP-170 protein is required for correct chromosome poleward movement in fission yeast.

Goldstone S et al. PLoS One 2010 May 13;5(5):e10634

PMID:11739799 - Localization of fission yeast type II myosin, Myo2, to the cytokinetic actin ring is regulated by phosphorylation of a C-terminal coiled-coil domain and requires a functional septation initiation network.

Mulvihill DP et al. Mol Biol Cell 2001 Dec;12(12):4044-53

PMID:12242222 - The Scw1 RNA-binding domain protein regulates septation and cell-wall structure in fission yeast.

Karagiannis J et al. Genetics 2002 Sep;162(1):45-58

PMID:10799520 - Byr4 localizes to spindle-pole bodies in a cell cycle-regulated manner to control Cdc7 localization and septation in fission yeast.

Li C et al. J Biol Chem 2000 May 12;275(19):14381-7

PMID:11950932 - The spindle pole body protein Cdc11p links Sid4p to the fission yeast septation initiation network.

Tomlin GC et al. Mol Biol Cell 2002 Apr;13(4):1203-14

PMID:22419817 - The fission yeast septation initiation network (SIN) kinase, Sid2, is required for SIN asymmetry and regulates the SIN scaffold, Cdc11.

Feoktistova A et al. Mol Biol Cell 2012 May;23(9):1636-45

PMID:9398685 - Identification of a second myosin-II in Schizosaccharomyces pombe: Myp2p is conditionally required for cytokinesis.

Bezanilla M et al. Mol Biol Cell 1997 Dec;8(12):2693-705

PMID:8334988 - The S. pombe cdc16 gene is required both for maintenance of p34cdc2 kinase activity and regulation of septum formation: a link between mitosis and cytokinesis?

Fankhauser C et al. EMBO J 1993 Jul;12(7):2697-704

PMID:16787941 - The Schizosaccharomyces pombe septation initiation network (SIN) is required for spore formation in meiosis.

Krapp A et al. J Cell Sci 2006 Jul 15;119(Pt 14):2882-91

PMID:12479804 - Dma1 prevents mitotic exit and cytokinesis by inhibiting the septation initiation network (SIN).

Guertin DA et al. Dev Cell 2002 Dec;3(6):779-90

PMID:16085489 - The 14-3-3 protein rad24p modulates function of the cdc14p family phosphatase clp1p/flp1p in fission yeast.

Mishra M et al. Curr Biol 2005 Aug 09;15(15):1376-83

PMID:25501814 - The septation initiation network controls the assembly of nodes containing Cdr2p for cytokinesis in fission yeast.

Pu KM et al. J Cell Sci 2015 Feb 01;128(3):441-6

PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.

Marguerat S et al. Cell 2012 Oct 26;151(3):671-83

PMID:11707284 - The protein phosphatase 2A B'-regulatory subunit par1p is implicated in regulation of the S. pombe septation initiation network.

Le Goff X et al. FEBS Lett 2001 Nov 09;508(1):136-42

PMID:10392445 - Eleven novel sep genes of Schizosaccharomyces pombe required for efficient cell separation and sexual differentiation.

Grallert A et al. Yeast 1999 Jun 15;15(8):669-86

PMID:20603077 - Dephosphorylation of F-BAR protein Cdc15 modulates its conformation and stimulates its scaffolding activity at the cell division site.

Roberts-Galbraith RH et al. Mol Cell 2010 Jul 09;39(1):86-99

PMID:11250892 - The role of Plo1 kinase in mitotic commitment and septation in Schizosaccharomyces pombe.

Tanaka K et al. EMBO J 2001 Mar 15;20(6):1259-70

PMID:17502918 - A cyclin-dependent kinase that promotes cytokinesis through modulating phosphorylation of the carboxy terminal domain of the RNA Pol II Rpb1p sub-unit.

Karagiannis J et al. PLoS One 2007 May 09;2(5):e433

PMID:10924454 - A new genetic method for isolating functionally interacting genes: high plo1(+)-dependent mutants and their suppressors define genes in mitotic and septation pathways in fission yeast.

Cullen CF et al. Genetics 2000 Aug;155(4):1521-34

PMID:15548596 - Analysis of mutant phenotypes and splicing defects demonstrates functional collaboration between the large and small subunits of the essential splicing factor U2AF in vivo.

Webb CJ et al. Mol Biol Cell 2005 Feb;16(2):584-96

PMID:16772338 - The fission yeast Chs2 protein interacts with the type-II myosin Myo3p and is required for the integrity of the actomyosin ring.

Martín-García R et al. J Cell Sci 2006 Jul 01;119(Pt 13):2768-79

PMID:38865179 - Transient PP2A SIP complex localization to mitotic SPBs for SIN inhibition is mediated solely by the Csc1 FHA domain.

Willet AH et al. Mol Biol Cell 2024 Jun 12;:mbcE24040196

PMID:10775265 - The role of the sid1p kinase and cdc14p in regulating the onset of cytokinesis in fission yeast.

Guertin DA et al. EMBO J 2000 Apr 17;19(8):1803-15

PMID:9638658 - Byr4, a dosage-dependent regulator of cytokinesis in S. pombe, interacts with a possible small GTPase pathway including Spg1 and Cdc16.

Jwa M et al. Mol Cells 1998 Apr 30;8(2):240-5

PMID:24920823 - Cdk1 promotes cytokinesis in fission yeast through activation of the septation initiation network.

Rachfall N et al. Mol Biol Cell 2014 Aug 01;25(15):2250-9

PMID:12546793 - Mitotic hyperphosphorylation of the fission yeast SIN scaffold protein cdc11p is regulated by the protein kinase cdc7p.

Krapp A et al. Curr Biol 2003 Jan 21;13(2):168-72

PMID:22905165 - The integrity of the cytokinesis machinery under stress conditions requires the glucan synthase Bgs1p and its regulator Cfh3p.

Sharifmoghadam MR et al. PLoS One 2012;7(8):e42726

PMID:23087209 - The kinesin-14 Klp2 is negatively regulated by the SIN for proper spindle elongation and telophase nuclear positioning.

Mana-Capelli S et al. Mol Biol Cell 2012 Dec;23(23):4592-600

PMID:33823663 - A TOR (target of rapamycin) and nutritional phosphoproteome of fission yeast reveals novel targets in networks conserved in humans.

Halova L et al. Open Biol 2021 Apr;11(4):200405

PMID:762020 - Uncontrolled septation in a cell division cycle mutant of the fission yeast Schizosaccharomyces pombe.

Minet M et al. J Bacteriol 1979 Jan;137(1):440-6

PMID:10196225 - Regions of Byr4, a regulator of septation in fission yeast, that bind Spg1 or Cdc16 and form a two-component GTPase-activating protein with Cdc16.

Furge KA et al. J Biol Chem 1999 Apr 16;274(16):11339-43

PMID:1527180 - Genetic interactions in the control of septation in Schizosaccharomyces pombe.

Marks J et al. J Cell Sci 1992 Apr;101 ( Pt 4):801-8

PMID:35099006 - Fission yeast polycystin Pkd2p promotes cell size expansion and antagonizes the Hippo-related SIN pathway.

Sinha D et al. J Cell Sci 2022 Feb 15;135(4)

PMID:10462526 - The S. pombe zfs1 gene is required to prevent septation if mitotic progression is inhibited.

Beltraminelli N et al. J Cell Sci 1999 Sep;112 Pt 18:3103-14

PMID:16823372 - ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe.

Matsuyama A et al. Nat Biotechnol 2006 Jul;24(7):841-7

PMID:22119525 - SIN-inhibitory phosphatase complex promotes Cdc11p dephosphorylation and propagates SIN asymmetry in fission yeast.

Singh NS et al. Curr Biol 2011 Dec 06;21(23):1968-78

PMID:24790095 - Characterization of the roles of Blt1p in fission yeast cytokinesis.

Goss JW et al. Mol Biol Cell 2014 Jul 01;25(13):1946-57

PMID:15062098 - Sid4p-Cdc11p assembles the septation initiation network and its regulators at the S. pombe SPB.

Morrell JL et al. Curr Biol 2004 Apr 06;14(7):579-84

PMID:26702831 - Oligomerization but Not Membrane Bending Underlies the Function of Certain F-BAR Proteins in Cell Motility and Cytokinesis.

McDonald NA et al. Dev Cell 2015 Dec 21;35(6):725-36

PMID:15265986 - The Clp1p/Flp1p phosphatase ensures completion of cytokinesis in response to minor perturbation of the cell division machinery in Schizosaccharomyces pombe.

Mishra M et al. J Cell Sci 2004 Aug 01;117(Pt 17):3897-910

PMID:19736319 - Proper timing of cytokinesis is regulated by Schizosaccharomyces pombe Etd1.

García-Cortés JC et al. J Cell Biol 2009 Sep 07;186(5):739-53

PMID:21931816 - Global gene expression analysis of fission yeast mutants impaired in Ser-2 phosphorylation of the RNA pol II carboxy terminal domain.

Saberianfar R et al. PLoS One 2011;6(9):e24694

PMID:29996109 - Quantitative Phosphoproteomics Reveals the Signaling Dynamics of Cell-Cycle Kinases in the Fission Yeast Schizosaccharomyces pombe.

Swaffer MP et al. Cell Rep 2018 Jul 10;24(2):503-514

PMID:10805785 - Sid4p is required to localize components of the septation initiation pathway to the spindle pole body in fission yeast.

Chang L et al. Proc Natl Acad Sci U S A 2000 May 09;97(10):5249-54

PMID:11271422 - Bypass of the requirement for cdc16p GAP function in Schizosaccharomyces pombe by mutation of the septation initiation network genes.

Fournier N et al. Arch Microbiol 2001 Jan;175(1):62-9

PMID:24763107 - Absolute proteome and phosphoproteome dynamics during the cell cycle of Schizosaccharomyces pombe (Fission Yeast).

Carpy A et al. Mol Cell Proteomics 2014 Aug;13(8):1925-36

PMID:20876564 - Antagonistic roles of PP2A-Pab1 and Etd1 in the control of cytokinesis in fission yeast.

Lahoz A et al. Genetics 2010 Dec;186(4):1261-70

PMID:17614284 - The chromatin-remodeling factor FACT contributes to centromeric heterochromatin independently of RNAi.

Lejeune E et al. Curr Biol 2007 Jul 17;17(14):1219-24

PMID:16096637 - Fission yeast MO25 protein is localized at SPB and septum and is essential for cell morphogenesis.

Kanai M et al. EMBO J 2005 Sep 07;24(17):3012-25

PMID:10769201 - The S. pombe orthologue of the S. cerevisiae mob1 gene is essential and functions in signalling the onset of septum formation.

Salimova E et al. J Cell Sci 2000 May;113 ( Pt 10):1695-704

PMID:16687577 - Cell cycle-dependent roles for the FCH-domain protein Cdc15p in formation of the actomyosin ring in Schizosaccharomyces pombe.

Wachtler V et al. Mol Biol Cell 2006 Jul;17(7):3254-66

PMID:21712547 - Mitotic substrates of the kinase aurora with roles in chromatin regulation identified through quantitative phosphoproteomics of fission yeast.

Koch A et al. Sci Signal 2011 Jun 28;4(179):rs6

PMID:21511999 - Comparative functional genomics of the fission yeasts.

Rhind N et al. Science 2011 May 20;332(6032):930-6

PMID:9420333 - Asymmetric segregation on spindle poles of the Schizosaccharomyces pombe septum-inducing protein kinase Cdc7p.

Sohrmann M et al. Genes Dev 1998 Jan 01;12(1):84-94

PMID:30726745 - Fission Yeast NDR/LATS Kinase Orb6 Regulates Exocytosis via Phosphorylation of the Exocyst Complex.

Tay YD et al. Cell Rep 2019 Feb 05;26(6):1654-1667.e7

PMID:16325501 - The fission yeast MO25 protein functions in polar growth and cell separation.

Mendoza M et al. Eur J Cell Biol 2005 Dec;84(12):915-26

PMID:20805322 - The mitosis-to-interphase transition is coordinated by cross talk between the SIN and MOR pathways in Schizosaccharomyces pombe.

Ray S et al. J Cell Biol 2010 Sep 06;190(5):793-805

PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.

Hayles J et al. Open Biol 2013 May 22;3(5):130053

PMID:24838944 - Dma1-dependent degradation of SIN proteins during meiosis in Schizosaccharomyces pombe.

Krapp A et al. J Cell Sci 2014 Jul 15;127(Pt 14):3149-61

PMID:31509478 - The intrinsically disordered region of the cytokinetic F-BAR protein Cdc15 performs a unique essential function in maintenance of cytokinetic ring integrity.

Mangione MC et al. Mol Biol Cell 2019 Oct 15;30(22):2790-2801

PMID:10381387 - Asymmetry of the spindle pole bodies and spg1p GAP segregation during mitosis in fission yeast.

Cerutti L et al. J Cell Sci 1999 Jul;112 ( Pt 14):2313-21

PMID:18204818 - Identification of small molecules inducing apoptosis by cell-based assay using fission yeast deletion mutants.

Chung KS et al. Invest New Drugs 2008 Aug;26(4):299-307

PMID:15132994 - Recruitment of NIMA kinase shows that maturation of the S. pombe spindle-pole body occurs over consecutive cell cycles and reveals a role for NIMA in modulating SIN activity.

Grallert A et al. Genes Dev 2004 May 01;18(9):1007-21

PMID:19075108 - Assembly of normal actomyosin rings in the absence of Mid1p and cortical nodes in fission yeast.

Huang Y et al. J Cell Biol 2008 Dec 15;183(6):979-88

PMID:22681890 - Hierarchical modularity and the evolution of genetic interactomes across species.

Ryan CJ et al. Mol Cell 2012 Jun 08;46(5):691-704

PMID:18256290 - Schizosaccharomyces pombe Pxl1 is a paxillin homologue that modulates Rho1 activity and participates in cytokinesis.

Pinar M et al. Mol Biol Cell 2008 Apr;19(4):1727-38

PMID:23874188 - Dynamics of SIN asymmetry establishment.

Bajpai A et al. PLoS Comput Biol 2013;9(7):e1003147

PMID:9742395 - Byr4 and Cdc16 form a two-component GTPase-activating protein for the Spg1 GTPase that controls septation in fission yeast.

Furge KA et al. Curr Biol 1998 Aug 27;8(17):947-54

PMID:22267499 - Characterization of ypa1 and ypa2, the Schizosaccharomyces pombe orthologs of the peptidyl proyl isomerases that activate PP2A, reveals a role for Ypa2p in the regulation of cytokinesis.

Goyal A et al. Genetics 2012 Apr;190(4):1235-50

PMID:36361590 - Defining the Functional Interactome of Spliceosome-Associated G-Patch Protein Gpl1 in the Fission Yeast Schizosaccharomyces pombe .

Selicky T et al. Int J Mol Sci 2022 Oct 24;23(21)

PMID:12186944 - Cytokinetic actomyosin ring formation and septation in fission yeast are dependent on the full recruitment of the polo-like kinase Plo1 to the spindle pole body and a functional spindle assembly checkpoint.

Mulvihill DP et al. J Cell Sci 2002 Sep 15;115(Pt 18):3575-86

PMID:38989013 - Characterization of temperature-sensitive alleles of Schizosaccharomyces pombe septation initiation network components.

Turner LA et al. MicroPubl Biol 2024;2024

PMID:15537703 - The nuclear kinase Lsk1p positively regulates the septation initiation network and promotes the successful completion of cytokinesis in response to perturbation of the actomyosin ring in Schizosaccharomyces pombe.

Karagiannis J et al. Mol Biol Cell 2005 Jan;16(1):358-71

PMID:23394829 - Identification of SIN pathway targets reveals mechanisms of crosstalk between NDR kinase pathways.

Gupta S et al. Curr Biol 2013 Feb 18;23(4):333-8

PMID:9203579 - The Spg1p GTPase is an essential, dosage-dependent inducer of septum formation in Schizosaccharomyces pombe.

Schmidt S et al. Genes Dev 1997 Jun 15;11(12):1519-34

PMID:26771498 - A Proteome-wide Fission Yeast Interactome Reveals Network Evolution Principles from Yeasts to Human.

Vo TV et al. Cell 2016 Jan 14;164(1-2):310-323

PMID:30853434 - NDR Kinase Sid2 Drives Anillin-like Mid1 from the Membrane to Promote Cytokinesis and Medial Division Site Placement.

Willet AH et al. Curr Biol 2019 Mar 18;29(6):1055-1063.e2

PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.

Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623

PMID:8978687 - The fission yeast dma1 gene is a component of the spindle assembly checkpoint, required to prevent septum formation and premature exit from mitosis if spindle function is compromised.

Murone M et al. EMBO J 1996 Dec 02;15(23):6605-16

PMID:8505375 - Mycelial and syncytial growth in Schizosaccharomyces pombe induced by novel septation mutations.

Sipiczki M et al. J Cell Sci 1993 Feb;104 ( Pt 2):485-93