protein coding gene - tpz1 (SPAC6F6.16c) - shelterin complex subunit Tpz1

Gene summary

Standard name: tpz1
Systematic ID: SPAC6F6.16c
Product: shelterin complex subunit Tpz1
Organism: Schizosaccharomyces pombe (fission yeast)
Synonyms: SPAC6F6.18c, mug169
UniProt ID: O14246
ORFeome ID: 11/11A11
Characterisation status: biological role published
Feature type: mRNA gene
Genomic location: chromosome I: 2762475..2765904 reverse strand

Annotation

Disease association

MONDO:0015780 - dyskeratosis congenita

References:

PB_REF:0000003

MONDO:0014690 - dyskeratosis congenita, autosomal dominant 6

References:

PB_REF:0000006

GO biological process

GO:0016233 - telomere capping

References:

PMID:18535244

GO:0000723 - telomere maintenance

References:

PMID:25330395

GO:0007004 - telomere maintenance via telomerase

References:

PMID:25131669

GO:1905324 - telomere-telomerase complex assembly

References:

PMID:27253066

GO cellular component

GO:0140445 - chromosome, telomeric repeat region

References:

PMID:18535244

GO:0070187 - shelterin complex

References:

GO:0005697 - telomerase holoenzyme complex

References:

GO_REF:0000002

GO:0000782 - telomere cap complex

References:

PMID:24925530

GO molecular function

GO:0005515 - protein binding

References:

GO:0042162 - telomeric repeat DNA binding

References:

GO_REF:0000002

Modification

MOD:00046 - O-phospho-L-serine

References:

PMID:29996109

MOD:01149 - sumoylated lysine

References:

Multi-locus phenotype

FYPO:0002702 - circularized chromosome

References:

Genotypes:

FYPO:0002907 - circularized chromosome during mitotic G1 phase

References:

PMID:26063574

Genotypes:

FYPO:0000080 - decreased cell population growth at low temperature

References:

PMID:26063574

Genotypes:

poz1delta rap1delta taz1delta tpz1-taz1[Knockdown]

FYPO:0003412 - decreased chromatin silencing at centromere outer repeat

References:

PMID:24240238

Genotypes:

dcr1delta tpz1delta

FYPO:0004604 - decreased chromatin silencing at subtelomere

References:

PMID:26063574

Genotypes:

poz1delta rap1delta taz1delta tpz1-taz1[Knockdown]

FYPO:0001355 - decreased vegetative cell population growth

References:

Genotypes:

FYPO:0002019 - elongated telomeres during vegetative growth

References:

Genotypes:

FYPO:0002141 - normal cell population growth at low temperature

References:

PMID:26063574

Genotypes:

FYPO:0003555 - normal chromatin silencing at subtelomere

References:

PMID:26063574

Genotypes:

FYPO:0002687 - normal telomere length during vegetative growth

References:

PMID:26063574

Genotypes:

FYPO:0003107 - progressively shortening telomeres during vegetative growth

References:

Genotypes:

FYPO:0000091 - sensitive to thiabendazole

References:

PMID:24240238

Genotypes:

dcr1delta tpz1delta

FYPO:0002239 - shortened telomeres during vegetative growth

References:

PMID:29160296

Genotypes:

ccq1delta tpz1-I501R(aa)

FYPO:0003106 - stable shortened telomeres during vegetative growth

References:

Genotypes:

FYPO:0005404 - telomeric regions absent from linear chromosomes

References:

Genotypes:

Protein features

PBO:0111871 - OB fold

References:

PMID:18535244

Qualitative gene expression

PomGeneEx:0000019 - protein level decreased

References:

PMID:39367033

PomGeneEx:0000011 - RNA level increased

References:

Quantitative gene expression

PBO:0011963 - RNA level

References:

PMID:23101633

Single locus phenotype

FYPO:0000059 - abnormal mitotic cell cycle

References:

PMID:23697806

Genotypes:

tpz1delta

FYPO:0005934 - abolished protein sumoylation during vegetative growth

References:

PMID:24925530

Genotypes:

tpz1-K242R(aa)

FYPO:0000705 - abolished protein-protein interaction

References:

Genotypes:

FYPO:0002702 - circularized chromosome

References:

PMID:25330395

Genotypes:

FYPO:0003412 - decreased chromatin silencing at centromere outer repeat

References:

PMID:24240238

Genotypes:

tpz1delta

FYPO:0004604 - decreased chromatin silencing at subtelomere

References:

PMID:25330395

Genotypes:

FYPO:0003803 - decreased protein localization to telomere

References:

Genotypes:

FYPO:0001645 - decreased protein-protein interaction

References:

Genotypes:

FYPO:0001355 - decreased vegetative cell population growth

References:

PMID:29774234

Genotypes:

tpz1-K242R(aa)

FYPO:0002019 - elongated telomeres during vegetative growth

References:

Genotypes:

FYPO:0001707 - increased mitotic DNA damage checkpoint activation

References:

PMID:24013504

Genotypes:

tpz1-L449A(aa)

FYPO:0004656 - increased protein localization to telomere during vegetative growth

References:

Genotypes:

FYPO:0001038 - increased protein phosphorylation during vegetative growth

References:

PMID:25330395

Genotypes:

FYPO:0001571 - increased protein-protein interaction

References:

Genotypes:

FYPO:0003529 - inviable after spore germination, multiple cell divisions, cell cycle arrest in mitotic interphase, elongated cells

References:

PMID:23697806

Genotypes:

tpz1delta

FYPO:0002061 - inviable vegetative cell population

References:

Genotypes:

tpz1delta

FYPO:0002887 - normal protein localization to telomere during vegetative growth

References:

Genotypes:

FYPO:0000703 - normal protein-protein interaction

References:

Genotypes:

FYPO:0002687 - normal telomere length during vegetative growth

References:

Genotypes:

FYPO:0001357 - normal vegetative cell population growth

References:

PMID:24925530

Genotypes:

tpz1-K242R(aa)

FYPO:0003107 - progressively shortening telomeres during vegetative growth

References:

Genotypes:

FYPO:0000091 - sensitive to thiabendazole

References:

PMID:24240238

Genotypes:

tpz1delta

FYPO:0002239 - shortened telomeres during vegetative growth

References:

Genotypes:

FYPO:0003106 - stable shortened telomeres during vegetative growth

References:

Genotypes:

FYPO:0001492 - viable elongated vegetative cell

References:

PMID:25330395

Genotypes:

FYPO:0003095 - viable elongated vegetative cell, with progressive elongation

References:

PMID:24013504

Genotypes:

tpz1-L449A(aa)

Taxonomic conservation

PBO:0011065 - conserved in eukaryotes

PBO:0011071 - conserved in eukaryotes only

PBO:0011064 - conserved in fungi

PBO:0011069 - conserved in metazoa

PBO:0011070 - conserved in vertebrates

PBO:0006222 - predominantly single copy (one to one)

Warnings

PBO:0000070 - gene structure updated

Protein features

ID	Name	InterPro name	DB name
PF10341	TPP1	TPP1/Est3	Pfam
PTHR14487		ACD	PANTHER
mobidb-lite-Disorder	disorder_prediction		MobiDB-Disorder
mobidb-lite-Polar	disorder_prediction		MobiDB-Polar
mobidb-lite-Polyampholyte	disorder_prediction		MobiDB-Polyampholyte

Orthologs

ACD (HGNC:25070) Homo sapiens
tpz1 (SJAG_01378) Schizosaccharomyces japonicus
EST3 (YIL009C-A) Saccharomyces cerevisiae

References / Literature

PMID:26771498 - A Proteome-wide Fission Yeast Interactome Reveals Network Evolution Principles from Yeasts to Human.: Vo TV et al. Cell 2016 Jan 14;164(1-2):310-323
PMID:25131669 - Telomerase activation after recruitment in fission yeast.: Armstrong CA et al. Curr Biol 2014 Sep 08;24(17):2006-11
PMID:29216371 - Fission yeast Ccq1 is a modulator of telomerase activity.: Armstrong CA et al. Nucleic Acids Res 2018 Jan 25;46(2):704-716
PMID:39367033 - Quantitative proteomics and phosphoproteomics profiling of meiotic divisions in the fission yeast Schizosaccharomyces pombe.: Sivakova B et al. Sci Rep 2024 Oct 04;14(1):23105
PMID:26063574 - Minishelterins separate telomere length regulation and end protection in fission yeast.: Pan L et al. Genes Dev 2015 Jun 01;29(11):1164-74
PMID:31131414 - Casein kinase 2 regulates telomere protein complex formation through Rap1 phosphorylation.: Inoue H et al. Nucleic Acids Res 2019 Jul 26;47(13):6871-6884
PMID:22681890 - Hierarchical modularity and the evolution of genetic interactomes across species.: Ryan CJ et al. Mol Cell 2012 Jun 08;46(5):691-704
PMID:26354422 - Ccq1-Tpz1TPP1 interaction facilitates telomerase and SHREC association with telomeres in fission yeast.: Moser BA et al. Mol Biol Cell 2015 Nov 01;26(21):3857-66
PMID:29160296 - Structure of the fission yeast S. pombe telomeric Tpz1-Poz1-Rap1 complex.: Xue J et al. Cell Res 2017 Dec;27(12):1503-1520
GO_REF:0000002 - Comments
PMID:26896847 - Ensembl comparative genomics resources.: Herrero J et al. Database (Oxford) 2016;2016
PMID:29996109 - Quantitative Phosphoproteomics Reveals the Signaling Dynamics of Cell-Cycle Kinases in the Fission Yeast Schizosaccharomyces pombe.: Swaffer MP et al. Cell Rep 2018 Jul 10;24(2):503-514
PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.: Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623
PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.: Marguerat S et al. Cell 2012 Oct 26;151(3):671-83
PMID:24013504 - Tpz1 controls a telomerase-nonextendible telomeric state and coordinates switching to an extendible state via Ccq1.: Jun HI et al. Genes Dev 2013 Sep 01;27(17):1917-31
PMID:25330395 - Tpz1-Ccq1 and Tpz1-Poz1 interactions within fission yeast shelterin modulate Ccq1 Thr93 phosphorylation and telomerase recruitment.: Harland JL et al. PLoS Genet 2014 Oct;10(10):e1004708
PMID:26365187 - Dissecting Fission Yeast Shelterin Interactions via MICro-MS Links Disruption of Shelterin Bridge to Tumorigenesis.: Liu J et al. Cell Rep 2015 Sep 29;12(12):2169-80
PMID:26988418 - The proper connection between shelterin components is required for telomeric heterochromatin assembly.: Wang J et al. Genes Dev 2016 Apr 01;30(7):827-39
PMID:16537923 - Sterol regulatory element binding protein is a principal regulator of anaerobic gene expression in fission yeast.: Todd BL et al. Mol Cell Biol 2006 Apr;26(7):2817-31
PMID:26670050 - Regulation of mRNA Levels by Decay-Promoting Introns that Recruit the Exosome Specificity Factor Mmi1.: Kilchert C et al. Cell Rep 2015 Dec 22;13(11):2504-2515
PMID:29774234 - The fission yeast Stn1-Ten1 complex limits telomerase activity via its SUMO-interacting motif and promotes telomeres replication.: Matmati S et al. Sci Adv 2018 May;4(5):eaar2740
PMID:34255844 - The cooperative assembly of shelterin bridge provides a kinetic gateway that controls telomere length homeostasis.: Liu J et al. Nucleic Acids Res 2021 Aug 20;49(14):8110-8119
PMID:41201383 - Combinatorial Analyses of Pre-mRNA Splicing-Defective Mutants Reveal Differential Quantitative Requirements for Shelterin in Distinct Telomere Functions.: Takeuchi M et al. Genes Cells 2025 Nov;30(6):e70064
PMID:28807855 - Spatial Organization and Molecular Interactions of the Schizosaccharomyces pombe Ccq1-Tpz1-Poz1 Shelterin Complex.: Scott H et al. J Mol Biol 2017 Sep 15;429(19):2863-2872
PMID:24925530 - Tpz1TPP1 SUMOylation reveals evolutionary conservation of SUMO-dependent Stn1 telomere association.: Garg M et al. EMBO Rep 2014 Aug;15(8):871-7
Pfam:PF10341 - Unknown title
PMID:34706246 - Cross talk between the upstream exon-intron junction and Prp2 facilitates splicing of non-consensus introns.: Hümmer S et al. Cell Rep 2021 Oct 26;37(4):109893
PMID:12161753 - The transcriptional program of meiosis and sporulation in fission yeast.: Mata J et al. Nat Genet 2002 Sep;32(1):143-7
PMID:35849625 - Structural insights into Pot1-ssDNA, Pot1-Tpz1 and Tpz1-Ccq1 Interactions within fission yeast shelterin complex.: Sun H et al. PLoS Genet 2022 Jul;18(7):e1010308
PB_REF:0000003 - Disease Association Curation
PMID:21911358 - DNA-induced dimerization of the single-stranded DNA binding telomeric protein Pot1 from Schizosaccharomyces pombe.: Nandakumar J et al. Nucleic Acids Res 2012 Jan;40(1):235-44
PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.: Hayles J et al. Open Biol 2013 May 22;3(5):130053
PMID:24711392 - SUMOylation regulates telomere length by targeting the shelterin subunit Tpz1(Tpp1) to modulate shelterin-Stn1 interaction in fission yeast.: Miyagawa K et al. Proc Natl Acad Sci U S A 2014 Apr 22;111(16):5950-5
PMID:27253066 - Multi-step coordination of telomerase recruitment in fission yeast through two coupled telomere-telomerase interfaces.: Hu X et al. Elife 2016 Jun 02;5
PMID:18535244 - Fission yeast Pot1-Tpp1 protects telomeres and regulates telomere length.: Miyoshi T et al. Science 2008 Jun 06;320(5881):1341-4
PB_REF:0000006 - Disease associations from Monarch via human-pombe orthologs
PMID:26537787 - Targeting of SUMO substrates to a Cdc48-Ufd1-Npl4 segregase and STUbL pathway in fission yeast.: Køhler JB et al. Nat Commun 2015 Nov 05;6:8827
PMID:29149597 - Structural Basis for Shelterin Bridge Assembly.: Kim JK et al. Mol Cell 2017 Nov 16;68(4):698-714.e5
PMID:22101932 - Tel1ATM and Rad3ATR kinases promote Ccq1-Est1 interaction to maintain telomeres in fission yeast.: Moser BA et al. Nat Struct Mol Biol 2011 Nov 20;18(12):1408-13
PMID:24240238 - Elimination of shelterin components bypasses RNAi for pericentric heterochromatin assembly.: Tadeo X et al. Genes Dev 2013 Nov 15;27(22):2489-99
PMID:39476757 - Characterization of Ksg1 protein kinase-dependent phosphoproteome in the fission yeast S. pombe.: Cipak L et al. Biochem Biophys Res Commun 2024 Oct 25;736:150895
PMID:30796050 - Ssu72 phosphatase is a conserved telomere replication terminator.: Escandell JM et al. EMBO J 2019 Apr 01;38(7)
PMID:29997179 - Shelterin promotes tethering of late replication origins to telomeres for replication-timing control.: Ogawa S et al. EMBO J 2018 Aug 01;37(15)