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protein coding gene - tpz1 (SPAC6F6.16c) - shelterin complex subunit Tpz1

Gene summary

Standard name
tpz1
Systematic ID
SPAC6F6.16c
Product
shelterin complex subunit Tpz1
Organism
Schizosaccharomyces pombe (fission yeast)
Synonyms
SPAC6F6.18c, mug169
UniProt ID
O14246
ORFeome ID
11/11A11
Characterisation status
biological role published
Feature type
mRNA gene
Genomic location
chromosome I: 2762475..2765904 reverse strand

Annotation

Disease association

MONDO:0015780 - dyskeratosis congenita

References:

MONDO:0014690 - dyskeratosis congenita, autosomal dominant 6

References:

GO biological process

GO:0016233 - telomere capping

References:

GO:0000723 - telomere maintenance

References:

GO:0007004 - telomere maintenance via telomerase

References:

GO:1905324 - telomere-telomerase complex assembly

References:

GO cellular component

GO:0140445 - chromosome, telomeric repeat region

References:

GO:0070187 - shelterin complex

References:

GO:0005697 - telomerase holoenzyme complex

References:

GO:0000782 - telomere cap complex

References:

GO molecular function

GO:0005515 - protein binding

References:

GO:0042162 - telomeric DNA binding

References:

Modification

MOD:00046 - O-phospho-L-serine

References:

MOD:01149 - sumoylated lysine

References:

Multi-locus phenotype

FYPO:0002702 - circularized chromosome

References:

Genotypes:

FYPO:0002907 - circularized chromosome during mitotic G1 phase

References:

Genotypes:

FYPO:0000080 - decreased cell population growth at low temperature

References:

Genotypes:

FYPO:0003412 - decreased chromatin silencing at centromere outer repeat

References:

Genotypes:

FYPO:0004604 - decreased chromatin silencing at subtelomere

References:

Genotypes:

FYPO:0001355 - decreased vegetative cell population growth

References:

Genotypes:

FYPO:0002019 - elongated telomeres during vegetative growth

References:

Genotypes:

FYPO:0002141 - normal cell population growth at low temperature

References:

Genotypes:

FYPO:0003555 - normal chromatin silencing at subtelomere

References:

Genotypes:

FYPO:0002687 - normal telomere length during vegetative growth

References:

Genotypes:

FYPO:0003107 - progressively shortening telomeres during vegetative growth

References:

Genotypes:

FYPO:0000091 - sensitive to thiabendazole

References:

Genotypes:

FYPO:0002239 - shortened telomeres during vegetative growth

References:

Genotypes:

FYPO:0003106 - stable shortened telomeres during vegetative growth

References:

Genotypes:

FYPO:0005404 - telomeric regions absent from linear chromosomes

References:

Genotypes:

Protein features

PBO:0111871 - OB fold

References:

Qualitative gene expression

PomGeneEx:0000019 - protein level decreased

References:

PomGeneEx:0000011 - RNA level increased

References:

Quantitative gene expression

PBO:0011963 - RNA level

References:

Single locus phenotype

FYPO:0000059 - abnormal mitotic cell cycle

References:

Genotypes:

FYPO:0005934 - abolished protein sumoylation during vegetative growth

References:

Genotypes:

FYPO:0000705 - abolished protein-protein interaction

References:

Genotypes:

FYPO:0002702 - circularized chromosome

References:

Genotypes:

FYPO:0003412 - decreased chromatin silencing at centromere outer repeat

References:

Genotypes:

FYPO:0004604 - decreased chromatin silencing at subtelomere

References:

Genotypes:

FYPO:0003803 - decreased protein localization to telomere

References:

Genotypes:

FYPO:0001645 - decreased protein-protein interaction

References:

Genotypes:

FYPO:0001355 - decreased vegetative cell population growth

References:

Genotypes:

FYPO:0002019 - elongated telomeres during vegetative growth

References:

Genotypes:

FYPO:0001707 - increased mitotic DNA damage checkpoint activation

References:

Genotypes:

FYPO:0004656 - increased protein localization to telomere during vegetative growth

References:

Genotypes:

FYPO:0001038 - increased protein phosphorylation during vegetative growth

References:

Genotypes:

FYPO:0001571 - increased protein-protein interaction

References:

Genotypes:

FYPO:0003529 - inviable after spore germination, multiple cell divisions, cell cycle arrest in mitotic interphase, elongated cells

References:

Genotypes:

FYPO:0002061 - inviable vegetative cell population

References:

Genotypes:

FYPO:0002887 - normal protein localization to telomere during vegetative growth

References:

Genotypes:

FYPO:0000703 - normal protein-protein interaction

References:

Genotypes:

FYPO:0002687 - normal telomere length during vegetative growth

References:

Genotypes:

FYPO:0001357 - normal vegetative cell population growth

References:

Genotypes:

FYPO:0003107 - progressively shortening telomeres during vegetative growth

References:

Genotypes:

FYPO:0000091 - sensitive to thiabendazole

References:

Genotypes:

FYPO:0002239 - shortened telomeres during vegetative growth

References:

Genotypes:

FYPO:0003106 - stable shortened telomeres during vegetative growth

References:

Genotypes:

FYPO:0001492 - viable elongated vegetative cell

References:

Genotypes:

FYPO:0003095 - viable elongated vegetative cell, with progressive elongation

References:

Genotypes:

Taxonomic conservation

PBO:0011065 - conserved in eukaryotes

PBO:0011071 - conserved in eukaryotes only

PBO:0011064 - conserved in fungi

PBO:0011069 - conserved in metazoa

PBO:0011070 - conserved in vertebrates

PBO:0006222 - predominantly single copy (one to one)

Warnings

PBO:0000070 - gene structure updated

Protein features

IDNameInterPro nameDB name
PF10341TPP1TPP1/Est3PFAM
PTHR14487ADRENOCORTICAL DYSPLASIA PROTEIN ACDACDPANTHER
mobidb-lite-Disorderdisorder_predictionMOBIDB-Disorder
mobidb-lite-Polardisorder_predictionMOBIDB-Polar
mobidb-lite-Polyampholytedisorder_predictionMOBIDB-Polyampholyte

Orthologs

References / Literature

PMID:29774234 - The fission yeast Stn1-Ten1 complex limits telomerase activity via its SUMO-interacting motif and promotes telomeres replication.
Matmati S et al. Sci Adv 2018 May;4(5):eaar2740
PMID:39367033 - Quantitative proteomics and phosphoproteomics profiling of meiotic divisions in the fission yeast Schizosaccharomyces pombe.
Sivakova B et al. Sci Rep 2024 Oct 04;14(1):23105
PMID:29149597 - Structural Basis for Shelterin Bridge Assembly.
Kim JK et al. Mol Cell 2017 Nov 16;68(4):698-714.e5
PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.
Hayles J et al. Open Biol 2013 May 22;3(5):130053
PMID:18535244 - Fission yeast Pot1-Tpp1 protects telomeres and regulates telomere length.
Miyoshi T et al. Science 2008 Jun 06;320(5881):1341-4
Pfam:PF10341 - Unknown title
PMID:26771498 - A Proteome-wide Fission Yeast Interactome Reveals Network Evolution Principles from Yeasts to Human.
Vo TV et al. Cell 2016 Jan 14;164(1-2):310-323
PB_REF:0000003 - Disease Association Curation
PMID:23299958 - Finding the end: recruitment of telomerase to telomeres.
Nandakumar J et al. Nat Rev Mol Cell Biol 2013 Feb;14(2):69-82
PMID:29216371 - Fission yeast Ccq1 is a modulator of telomerase activity.
Armstrong CA et al. Nucleic Acids Res 2018 Jan 25;46(2):704-716
GO_REF:0000002 - Comments
PMID:24013504 - Tpz1 controls a telomerase-nonextendible telomeric state and coordinates switching to an extendible state via Ccq1.
Jun HI et al. Genes Dev 2013 Sep 01;27(17):1917-31
PMID:27253066 - Multi-step coordination of telomerase recruitment in fission yeast through two coupled telomere-telomerase interfaces.
Hu X et al. Elife 2016 Jun 02;5
PMID:24240238 - Elimination of shelterin components bypasses RNAi for pericentric heterochromatin assembly.
Tadeo X et al. Genes Dev 2013 Nov 15;27(22):2489-99
PMID:26988418 - The proper connection between shelterin components is required for telomeric heterochromatin assembly.
Wang J et al. Genes Dev 2016 Apr 01;30(7):827-39
PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.
Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623
PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.
Marguerat S et al. Cell 2012 Oct 26;151(3):671-83
PMID:22101932 - Tel1ATM and Rad3ATR kinases promote Ccq1-Est1 interaction to maintain telomeres in fission yeast.
Moser BA et al. Nat Struct Mol Biol 2011 Nov 20;18(12):1408-13
PB_REF:0000006 - Disease associations from Monarch via human-pombe orthologs
PMID:21911358 - DNA-induced dimerization of the single-stranded DNA binding telomeric protein Pot1 from Schizosaccharomyces pombe.
Nandakumar J et al. Nucleic Acids Res 2012 Jan;40(1):235-44
PMID:26063574 - Minishelterins separate telomere length regulation and end protection in fission yeast.
Pan L et al. Genes Dev 2015 Jun 01;29(11):1164-74
PMID:29160296 - Structure of the fission yeast S. pombe telomeric Tpz1-Poz1-Rap1 complex.
Xue J et al. Cell Res 2017 Dec;27(12):1503-1520
PMID:26670050 - Regulation of mRNA Levels by Decay-Promoting Introns that Recruit the Exosome Specificity Factor Mmi1.
Kilchert C et al. Cell Rep 2015 Dec 22;13(11):2504-2515
PMID:26537787 - Targeting of SUMO substrates to a Cdc48-Ufd1-Npl4 segregase and STUbL pathway in fission yeast.
Køhler JB et al. Nat Commun 2015 Nov 05;6:8827
PMID:30796050 - Ssu72 phosphatase is a conserved telomere replication terminator.
Escandell JM et al. EMBO J 2019 Apr 01;38(7)
PMID:24711392 - SUMOylation regulates telomere length by targeting the shelterin subunit Tpz1(Tpp1) to modulate shelterin-Stn1 interaction in fission yeast.
Miyagawa K et al. Proc Natl Acad Sci U S A 2014 Apr 22;111(16):5950-5
PMID:26354422 - Ccq1-Tpz1TPP1 interaction facilitates telomerase and SHREC association with telomeres in fission yeast.
Moser BA et al. Mol Biol Cell 2015 Nov 01;26(21):3857-66
PMID:29997179 - Shelterin promotes tethering of late replication origins to telomeres for replication-timing control.
Ogawa S et al. EMBO J 2018 Aug 01;37(15)
PMID:24925530 - Tpz1TPP1 SUMOylation reveals evolutionary conservation of SUMO-dependent Stn1 telomere association.
Garg M et al. EMBO Rep 2014 Aug;15(8):871-7
PMID:22681890 - Hierarchical modularity and the evolution of genetic interactomes across species.
Ryan CJ et al. Mol Cell 2012 Jun 08;46(5):691-704
PMID:35849625 - Structural insights into Pot1-ssDNA, Pot1-Tpz1 and Tpz1-Ccq1 Interactions within fission yeast shelterin complex.
Sun H et al. PLoS Genet 2022 Jul;18(7):e1010308
PMID:25131669 - Telomerase activation after recruitment in fission yeast.
Armstrong CA et al. Curr Biol 2014 Sep 08;24(17):2006-11
PMID:26365187 - Dissecting Fission Yeast Shelterin Interactions via MICro-MS Links Disruption of Shelterin Bridge to Tumorigenesis.
Liu J et al. Cell Rep 2015 Sep 29;12(12):2169-80
PMID:25330395 - Tpz1-Ccq1 and Tpz1-Poz1 interactions within fission yeast shelterin modulate Ccq1 Thr93 phosphorylation and telomerase recruitment.
Harland JL et al. PLoS Genet 2014 Oct;10(10):e1004708
PMID:16537923 - Sterol regulatory element binding protein is a principal regulator of anaerobic gene expression in fission yeast.
Todd BL et al. Mol Cell Biol 2006 Apr;26(7):2817-31
PMID:29996109 - Quantitative Phosphoproteomics Reveals the Signaling Dynamics of Cell-Cycle Kinases in the Fission Yeast Schizosaccharomyces pombe.
Swaffer MP et al. Cell Rep 2018 Jul 10;24(2):503-514
PMID:28807855 - Spatial Organization and Molecular Interactions of the Schizosaccharomyces pombe Ccq1-Tpz1-Poz1 Shelterin Complex.
Scott H et al. J Mol Biol 2017 Sep 15;429(19):2863-2872
PMID:39476757 - Characterization of Ksg1 protein kinase-dependent phosphoproteome in the fission yeast S. pombe.
Cipak L et al. Biochem Biophys Res Commun 2024 Oct 25;736:150895
PMID:31131414 - Casein kinase 2 regulates telomere protein complex formation through Rap1 phosphorylation.
Inoue H et al. Nucleic Acids Res 2019 Jul 26;47(13):6871-6884
PMID:34255844 - The cooperative assembly of shelterin bridge provides a kinetic gateway that controls telomere length homeostasis.
Liu J et al. Nucleic Acids Res 2021 Aug 20;49(14):8110-8119
PMID:26896847 - Ensembl comparative genomics resources.
Herrero J et al. Database (Oxford) 2016;2016