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protein coding gene - arc4 (SPAC6G9.07c) - ARP2/3 actin-organizing complex subunit Arc4

Gene summary

Standard name
arc4
Systematic ID
SPAC6G9.07c
Product
ARP2/3 actin-organizing complex subunit Arc4
Organism
Schizosaccharomyces pombe (fission yeast)
Synonyms
arc19, arp10
UniProt ID
Q92352
ORFeome ID
11/11G09
Characterisation status
biological role published
Feature type
mRNA gene
Genomic location
chromosome I: 3258471..3259484 reverse strand

Annotation

Disease association

MONDO:0859324 - developmental delay, language impairment, and ocular abnormalities

References:

GO biological process

GO:0090135 - actin filament branching

References:

GO:0030041 - actin filament polymerization

References:

GO:0034314 - Arp2/3 complex-mediated actin nucleation

References:

GO:0072583 - clathrin-dependent endocytosis

References:

GO cellular component

GO:0030479 - actin cortical patch

References:

GO:0005885 - Arp2/3 protein complex

References:

GO:0005829 - cytosol

References:

GO:0005634 - nucleus

References:

GO molecular function

GO:0051015 - actin filament binding

References:

GO:0140378 - protein complex scaffold activity

References:

Modification

MOD:00046 - O-phospho-L-serine

References:

Quantitative gene expression

PBO:0006310 - protein level

References:

PBO:0011963 - RNA level

References:

Single locus phenotype

FYPO:0000313 - inviable after spore germination, without cell division, with abnormal germ tube morphology

References:

Genotypes:

FYPO:0002061 - inviable vegetative cell population

References:

Genotypes:

Taxonomic conservation

PBO:0011065 - conserved in eukaryotes

PBO:0011071 - conserved in eukaryotes only

PBO:0011064 - conserved in fungi

PBO:0011069 - conserved in metazoa

PBO:0011070 - conserved in vertebrates

PBO:0006222 - predominantly single copy (one to one)

Protein features

IDNameInterPro nameDB name
PF05856ARPC4ARPC4Pfam
G3DSA:3.30.1460.20:FF:000001CATH-FunFam
G3DSA:3.30.1460.20ARPC2/4CATH-Gene3D
SSF69645ARPC2/4SUPERFAMILY
PTHR22629ARPC4PANTHER
PIRSF039100ARPC4ARPC4PIRSF

Orthologs

References / Literature

GO_REF:0000024 - Manual transfer of experimentally-verified manual GO annotation data to orthologs by curator judgment of sequence similarity.
PMID:16087707 - Interactions of WASp, myosin-I, and verprolin with Arp2/3 complex during actin patch assembly in fission yeast.
Sirotkin V et al. J Cell Biol 2005 Aug 15;170(4):637-48
PMID:16823372 - ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe.
Matsuyama A et al. Nat Biotechnol 2006 Jul;24(7):841-7
PMID:18165685 - Pathway of actin filament branch formation by Arp2/3 complex.
Beltzner CC et al. J Biol Chem 2008 Mar 14;283(11):7135-44
PMID:21449051 - Schizosaccharomyces pombe Arc3 is a conserved subunit of the Arp2/3 complex required for polarity, actin organization, and endocytosis.
Cabrera R et al. Yeast 2011 Jun;28(6):495-503
PMID:22730331 - Dual recruitment of Cdc48 (p97)-Ufd1-Npl4 ubiquitin-selective segregase by small ubiquitin-like modifier protein (SUMO) and ubiquitin in SUMO-targeted ubiquitin ligase-mediated genome stability functions.
Nie M et al. J Biol Chem 2012 Aug 24;287(35):29610-9
PB_REF:0000006 - Disease associations from Monarch via human-pombe orthologs
PMID:21642440 - Actin filament bundling by fimbrin is important for endocytosis, cytokinesis, and polarization in fission yeast.
Skau CT et al. J Biol Chem 2011 Jul 29;286(30):26964-77
PMID:10588653 - A mutant of Arp2p causes partial disassembly of the Arp2/3 complex and loss of cortical actin function in fission yeast.
Morrell JL et al. Mol Biol Cell 1999 Dec;10(12):4201-15
PMID:36442092 - Mechanism of actin filament branch formation by Arp2/3 complex revealed by a high-resolution cryo-EM structureof the branch junction.
Chou SZ et al. Proc Natl Acad Sci U S A 2022 Dec 06;119(49):e2206722119
PMID:18640983 - Structure and biochemical properties of fission yeast Arp2/3 complex lacking the Arp2 subunit.
Nolen BJ et al. J Biol Chem 2008 Sep 26;283(39):26490-8
PMID:26771498 - A Proteome-wide Fission Yeast Interactome Reveals Network Evolution Principles from Yeasts to Human.
Vo TV et al. Cell 2016 Jan 14;164(1-2):310-323
PMID:30726745 - Fission Yeast NDR/LATS Kinase Orb6 Regulates Exocytosis via Phosphorylation of the Exocyst Complex.
Tay YD et al. Cell Rep 2019 Feb 05;26(6):1654-1667.e7
PMID:21511999 - Comparative functional genomics of the fission yeasts.
Rhind N et al. Science 2011 May 20;332(6032):930-6
PMID:25720772 - Quantitative phosphoproteomics reveals pathways for coordination of cell growth and division by the conserved fission yeast kinase pom1.
Kettenbach AN et al. Mol Cell Proteomics 2015 May;14(5):1275-87
PMID:38448439 - Cryo-EM structures reveal how phosphate release from Arp3 weakens actin filament branches formed by Arp2/3 complex.
Chavali SS et al. Nat Commun 2024 Mar 06;15(1):2059
GO_REF:0000002 - Comments
PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.
Hayles J et al. Open Biol 2013 May 22;3(5):130053
PMID:24763107 - Absolute proteome and phosphoproteome dynamics during the cell cycle of Schizosaccharomyces pombe (Fission Yeast).
Carpy A et al. Mol Cell Proteomics 2014 Aug;13(8):1925-36
PMID:32839613 - Cryo-EM reveals the transition of Arp2/3 complex from inactive to nucleation-competent state.
Shaaban M et al. Nat Struct Mol Biol 2020 Nov;27(11):1009-1016
PMID:19362000 - Cofilin dissociates Arp2/3 complex and branches from actin filaments.
Chan C et al. Curr Biol 2009 Apr 14;19(7):537-45
PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.
Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623
PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.
Marguerat S et al. Cell 2012 Oct 26;151(3):671-83