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protein coding gene - cfr1 (SPAC6G9.12) - exomer complex BRCT domain subunit Cfr1/Chs5

Gene summary

Standard name
cfr1
Systematic ID
SPAC6G9.12
Product
exomer complex BRCT domain subunit Cfr1/Chs5
Organism
Schizosaccharomyces pombe (fission yeast)
Synonyms
chs5
UniProt ID
Q92357
ORFeome ID
26/26C05
Characterisation status
biological role published
Feature type
mRNA gene
Genomic location
chromosome I: 3270197..3272367 forward strand

Annotation

Complementation

PBO:0013447 - does not functionally complement S. cerevisiae CHS5

References:

GO biological process

GO:0006895 - Golgi to endosome transport

References:

GO:0006893 - Golgi to plasma membrane transport

References:

GO:0006874 - intracellular calcium ion homeostasis

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GO:0030007 - intracellular potassium ion homeostasis

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GO:0006031 - chitin biosynthetic process

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GO:0009250 - glucan biosynthetic process

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GO cellular component

GO:0005829 - cytosol

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GO:0005769 - early endosome

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GO:0034044 - exomer complex

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GO:0005794 - Golgi apparatus

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GO:0005802 - trans-Golgi network

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GO molecular function

GO:0140312 - cargo adaptor activity

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Modification

MOD:00046 - O-phospho-L-serine

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MOD:00047 - O-phospho-L-threonine

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MOD:00696 - phosphorylated residue

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MOD:01148 - ubiquitinylated lysine

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Multi-locus phenotype

FYPO:0000349 - abnormal Golgi morphology

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Genotypes:

FYPO:0007677 - abnormal sterol distribution

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Genotypes:

FYPO:0004481 - abolished cell population growth at high temperature

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Genotypes:

FYPO:0005369 - abolished cell population growth at low temperature

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Genotypes:

FYPO:0008376 - abolished protein localization to cell cortex of cell tip during cellular response to osmotic stress

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Genotypes:

FYPO:0002159 - decreased 1,3-beta-D-glucan synthase activity

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Genotypes:

FYPO:0000082 - decreased cell population growth at high temperature

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Genotypes:

FYPO:0000080 - decreased cell population growth at low temperature

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Genotypes:

FYPO:0003771 - decreased protein localization to plasma membrane of cell tip during vegetative growth

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Genotypes:

FYPO:0001885 - decreased protein phosphorylation during salt stress

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Genotypes:

FYPO:0001355 - decreased vegetative cell population growth

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Genotypes:

FYPO:0007526 - increased protein phosphorylation during cellular response to salt stress

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Genotypes:

FYPO:0001038 - increased protein phosphorylation during vegetative growth

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Genotypes:

FYPO:0000123 - large vacuoles during vegetative growth

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Genotypes:

FYPO:0000674 - normal cell population growth at high temperature

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Genotypes:

FYPO:0002141 - normal cell population growth at low temperature

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Genotypes:

FYPO:0001020 - normal growth on calcium

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Genotypes:

FYPO:0007676 - normal intracellular sterol transport

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Genotypes:

FYPO:0000833 - normal protein level during vegetative growth

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Genotypes:

FYPO:0004467 - normal protein localization to cell tip during cellular response to salt stress

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Genotypes:

FYPO:0007678 - normal sterol distribution

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Genotypes:

FYPO:0001357 - normal vegetative cell population growth

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Genotypes:

FYPO:0000098 - sensitive to calcium

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Genotypes:

FYPO:0000079 - sensitive to caspofungin

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Genotypes:

FYPO:0000106 - sensitive to hygromycin B

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Genotypes:

FYPO:0001214 - sensitive to potassium chloride

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Genotypes:

FYPO:0007717 - sensitive to potassium nitrate

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Genotypes:

FYPO:0002788 - small vacuoles during vegetative growth

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Genotypes:

Protein features

PBO:0111779 - BRCT domain

Qualitative gene expression

PomGeneEx:0000018 - protein level increased

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Quantitative gene expression

PBO:0006310 - protein level

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PBO:0011963 - RNA level

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Single locus phenotype

FYPO:0001972 - abnormal cell separation after cytokinesis resulting in septated cell

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Genotypes:

FYPO:0000135 - abnormal plasma membrane sterol distribution

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Genotypes:

FYPO:0000443 - abnormal protein localization during vegetative growth

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Genotypes:

FYPO:0008376 - abolished protein localization to cell cortex of cell tip during cellular response to osmotic stress

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Genotypes:

FYPO:0000082 - decreased cell population growth at high temperature

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Genotypes:

FYPO:0009073 - decreased cell population growth on lysine nitrogen source

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Genotypes:

FYPO:0001082 - decreased cell wall beta-glucan level

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Genotypes:

FYPO:0006625 - decreased phosphatidylinositol-4,5-bisphosphate level in plasma membrane

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Genotypes:

FYPO:0004468 - decreased protein localization to cell tip during cellular response to salt stress

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Genotypes:

FYPO:0002376 - decreased protein phosphorylation during cellular response to osmotic stress

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Genotypes:

FYPO:0001885 - decreased protein phosphorylation during salt stress

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Genotypes:

FYPO:0001355 - decreased vegetative cell population growth

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Genotypes:

FYPO:0003279 - excess Golgi cisternae present

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Genotypes:

FYPO:0004203 - increased calcium import

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Genotypes:

FYPO:0003938 - increased cell population growth during glucose starvation

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Genotypes:

FYPO:0005261 - increased cell population growth on galactose carbon source

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Genotypes:

FYPO:0004167 - increased cell population growth on glycerol carbon source

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Genotypes:

FYPO:0001084 - increased cell wall alpha-glucan level

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Genotypes:

FYPO:0001505 - increased cellular phosphatidylserine level

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Genotypes:

FYPO:0008346 - increased cellular potassium level

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Genotypes:

FYPO:0008375 - increased phosphatidylinositol-4-phosphate level in the Golgi

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Genotypes:

FYPO:0008348 - increased protein localization to pre-vacuolar endosome

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Genotypes:

FYPO:0008347 - increased protein localization to trans-Golgi network

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Genotypes:

FYPO:0000539 - increased protein secretion during vegetative growth

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Genotypes:

FYPO:0004557 - increased vegetative cell population growth

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Genotypes:

FYPO:0001309 - increased viability in stationary phase

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Genotypes:

FYPO:0002141 - normal cell population growth at low temperature

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Genotypes:

FYPO:0000426 - normal endocytosis

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Genotypes:

FYPO:0000961 - normal growth on sorbitol

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Genotypes:

FYPO:0008374 - normal phosphatidylinositol-4-phosphate level in the Golgi

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Genotypes:

FYPO:0005168 - normal protein level during cellular response to salt stress

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Genotypes:

FYPO:0000833 - normal protein level during vegetative growth

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Genotypes:

FYPO:0003627 - normal protein localization

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Genotypes:

FYPO:0000644 - normal protein localization during vegetative growth

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Genotypes:

FYPO:0006385 - normal protein localization to cell tip during nitrogen starvation

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Genotypes:

FYPO:0004181 - normal protein localization to cell-cell contact point during mating

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Genotypes:

FYPO:0002674 - normal protein localization to plasma membrane

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Genotypes:

FYPO:0004422 - normal protein phosphorylation

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Genotypes:

FYPO:0004450 - normal protein phosphorylation during cellular response to calcium ion

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Genotypes:

FYPO:0004154 - normal protein phosphorylation during cellular response to heat

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Genotypes:

FYPO:0002290 - normal protein phosphorylation during cellular response to osmotic stress

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Genotypes:

FYPO:0001266 - normal protein phosphorylation during cellular response to salt stress

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Genotypes:

FYPO:0004247 - normal vacuolar morphology during vegetative growth

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Genotypes:

FYPO:0001357 - normal vegetative cell population growth

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Genotypes:

FYPO:0002693 - resistance to diamide

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Genotypes:

FYPO:0001583 - resistance to lithium

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Genotypes:

FYPO:0000725 - resistance to methyl methanesulfonate

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Genotypes:

FYPO:0005193 - resistance to torin1

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Genotypes:

FYPO:0001034 - resistance to tunicamycin

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Genotypes:

FYPO:0001501 - sensitive to brefeldin A

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Genotypes:

FYPO:0000096 - sensitive to cadmium

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Genotypes:

FYPO:0000098 - sensitive to calcium

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Genotypes:

FYPO:0000079 - sensitive to caspofungin

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Genotypes:

FYPO:0000104 - sensitive to cycloheximide

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Genotypes:

FYPO:0000843 - sensitive to dithiothreitol

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Genotypes:

FYPO:0007931 - sensitive to egtazic acid

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Genotypes:

FYPO:0000842 - sensitive to ethanol during vegetative growth

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Genotypes:

FYPO:0000087 - sensitive to hydrogen peroxide

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Genotypes:

FYPO:0000088 - sensitive to hydroxyurea

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Genotypes:

FYPO:0000106 - sensitive to hygromycin B

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Genotypes:

FYPO:0001719 - sensitive to lithium

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Genotypes:

FYPO:0009086 - sensitive to lithium chloride and sodium dodecyl sulfate

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Genotypes:

FYPO:0009088 - sensitive to magnesium chloride and sodium dodecyl sulfate

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Genotypes:

FYPO:0001214 - sensitive to potassium chloride

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Genotypes:

FYPO:0009082 - sensitive to potassium chloride and methyl methanesulfonate

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Genotypes:

FYPO:0007924 - sensitive to potassium chloride and sodium dodecyl sulfate

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Genotypes:

FYPO:0007717 - sensitive to potassium nitrate

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Genotypes:

FYPO:0000271 - sensitive to salt stress

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Genotypes:

FYPO:0009090 - sensitive to sodium chloride and sodium dodecyl sulfate

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Genotypes:

FYPO:0000797 - sensitive to tert-butyl hydroperoxide

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Genotypes:

FYPO:0001457 - sensitive to tunicamycin

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Genotypes:

FYPO:0000115 - sensitive to valproic acid

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Genotypes:

FYPO:0002060 - viable vegetative cell population

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Genotypes:

FYPO:0002177 - viable vegetative cell with normal cell morphology

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Genotypes:

Taxonomic conservation

PBO:0011065 - conserved in eukaryotes

PBO:0011064 - conserved in fungi

PBO:0011063 - conserved in fungi only

PBO:0006222 - predominantly single copy (one to one)

Protein features

IDNameInterPro nameDB name
PF00533BRCTBRCT_domPfam
PF16892CHS5_NChs5_NPfam
PF16893fn3_2Fn3_2Pfam
cd00063FN3FN3_domCDD
cd13945Chs5_NChs5_NCDD
PS50172BRCTBRCT_domPROSITE profiles
PS50853FN3FN3_domPROSITE profiles
SM00060FN3FN3_domSMART
SM00292BRCTBRCT_domSMART
G3DSA:2.60.40.10Ig-like_foldCATH-Gene3D
G3DSA:3.40.50.10190BRCT_dom_sfCATH-Gene3D
G3DSA:6.20.120.50CATH-Gene3D
SSF49265FN3_sfSUPERFAMILY
SSF52113BRCT_dom_sfSUPERFAMILY
PTHR47351Chs5PANTHER
CoilCOILS
mobidb-lite-Disorderdisorder_predictionMobiDB-Disorder
mobidb-lite-Low-complexitydisorder_predictionMobiDB-Low-complexity
mobidb-lite-Negative-Polyelectrolytedisorder_predictionMobiDB-Negative-Polyelectrolyte
mobidb-lite-Polardisorder_predictionMobiDB-Polar
mobidb-lite-Polyampholytedisorder_predictionMobiDB-Polyampholyte

Orthologs

References / Literature

PMID:16598689 - The Schizosaccharomyces pombe cfr1+ gene participates in mating through a new pathway that is independent of fus1+.
Cartagena-Lirola H et al. Yeast 2006 Apr 15;23(5):375-88
PMID:16823372 - ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe.
Matsuyama A et al. Nat Biotechnol 2006 Jul;24(7):841-7
PMID:21113731 - The FN3 and BRCT motifs in the exomer component Chs5p define a conserved module that is necessary and sufficient for its function.
Martín-García R et al. Cell Mol Life Sci 2011 Sep;68(17):2907-17
PMID:34349749 - Exomer Is Part of a Hub Where Polarized Secretion and Ionic Stress Connect.
Moro S et al. Front Microbiol 2021;12:708354
PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.
Marguerat S et al. Cell 2012 Oct 26;151(3):671-83
PMID:22681890 - Hierarchical modularity and the evolution of genetic interactomes across species.
Ryan CJ et al. Mol Cell 2012 Jun 08;46(5):691-704
PMID:24763107 - Absolute proteome and phosphoproteome dynamics during the cell cycle of Schizosaccharomyces pombe (Fission Yeast).
Carpy A et al. Mol Cell Proteomics 2014 Aug;13(8):1925-36
PMID:28218250 - Chromatin remodeller Fun30 Fft3 induces nucleosome disassembly to facilitate RNA polymerase II elongation.
Lee J et al. Nat Commun 2017 Feb 20;8:14527
PMID:24514900 - Membrane organization and cell fusion during mating in fission yeast requires multipass membrane protein Prm1.
Curto MÁ et al. Genetics 2014 Apr;196(4):1059-76
PMID:29996109 - Quantitative Phosphoproteomics Reveals the Signaling Dynamics of Cell-Cycle Kinases in the Fission Yeast Schizosaccharomyces pombe.
Swaffer MP et al. Cell Rep 2018 Jul 10;24(2):503-514
PMID:21511999 - Comparative functional genomics of the fission yeasts.
Rhind N et al. Science 2011 May 20;332(6032):930-6
PMID:27298342 - Identification of S-phase DNA damage-response targets in fission yeast reveals conservation of damage-response networks.
Willis NA et al. Proc Natl Acad Sci U S A 2016 Jun 28;113(26):E3676-85
PMID:32320462 - Sterol biosensor reveals LAM-family Ltc1-dependent sterol flow to endosomes upon Arp2/3 inhibition.
Marek M et al. J Cell Biol 2020 Jun 01;219(6)
PMID:33823663 - A TOR (target of rapamycin) and nutritional phosphoproteome of fission yeast reveals novel targets in networks conserved in humans.
Halova L et al. Open Biol 2021 Apr;11(4):200405
PMID:39540318 - Pck2 association with the plasma membrane and efficient response of the cell integrity pathway require regulation of PI4P homeostasis by exomer.
Moscoso-Romero E et al. Open Biol 2024 Nov;14(11):240101
PMID:37787768 - Broad functional profiling of fission yeast proteins using phenomics and machine learning.
Rodríguez-López M et al. Elife 2023 Oct 03;12
PMID:34250083 - Barcode sequencing and a high-throughput assay for chronological lifespan uncover ageing-associated genes in fission yeast.
Romila CA et al. Microb Cell 2021 Jul 05;8(7):146-160
PMID:27974503 - Traffic Through the Trans-Golgi Network and the Endosomal System Requires Collaboration Between Exomer and Clathrin Adaptors in Fission Yeast.
Hoya M et al. Genetics 2017 Feb;205(2):673-690
PMID:28281664 - Genetic interactions and functional analyses of the fission yeast gsk3 and amk2 single and double mutants defective in TORC1-dependent processes.
Rallis C et al. Sci Rep 2017 Mar 10;7:44257
PMID:39367033 - Quantitative proteomics and phosphoproteomics profiling of meiotic divisions in the fission yeast Schizosaccharomyces pombe.
Sivakova B et al. Sci Rep 2024 Oct 04;14(1):23105
PMID:26771498 - A Proteome-wide Fission Yeast Interactome Reveals Network Evolution Principles from Yeasts to Human.
Vo TV et al. Cell 2016 Jan 14;164(1-2):310-323
PMID:19627505 - The tetraspan protein Dni1p is required for correct membrane organization and cell wall remodelling during mating in Schizosaccharomyces pombe.
Clemente-Ramos JA et al. Mol Microbiol 2009 Aug;73(4):695-709
PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.
Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623
PMID:19547744 - Evolution of phosphoregulation: comparison of phosphorylation patterns across yeast species.
Beltrao P et al. PLoS Biol 2009 Jun 16;7(6):e1000134
PMID:39705284 - Proteomic and phosphoproteomic analyses reveal that TORC1 is reactivated by pheromone signaling during sexual reproduction in fission yeast.
Bérard M et al. PLoS Biol 2024 Dec 20;22(12):e3002963
PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.
Hayles J et al. Open Biol 2013 May 22;3(5):130053
PMID:16855022 - Chs5/6 complex: a multiprotein complex that interacts with and conveys chitin synthase III from the trans-Golgi network to the cell surface.
Sanchatjate S et al. Mol Biol Cell 2006 Oct;17(10):4157-66
PMID:21712547 - Mitotic substrates of the kinase aurora with roles in chromatin regulation identified through quantitative phosphoproteomics of fission yeast.
Koch A et al. Sci Signal 2011 Jun 28;4(179):rs6
PMID:25720772 - Quantitative phosphoproteomics reveals pathways for coordination of cell growth and division by the conserved fission yeast kinase pom1.
Kettenbach AN et al. Mol Cell Proteomics 2015 May;14(5):1275-87
PMID:37970674 - SUMOylation regulates Lem2 function in centromere clustering and silencing.
Strachan J et al. J Cell Sci 2023 Dec 01;136(23)
GO_REF:0000033 - Annotation inferences using phylogenetic trees
PMID:26412298 - A Degenerate Cohort of Yeast Membrane Trafficking DUBs Mediates Cell Polarity and Survival.
Beckley JR et al. Mol Cell Proteomics 2015 Dec;14(12):3132-41
PMID:30726745 - Fission Yeast NDR/LATS Kinase Orb6 Regulates Exocytosis via Phosphorylation of the Exocyst Complex.
Tay YD et al. Cell Rep 2019 Feb 05;26(6):1654-1667.e7