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protein coding gene - alg3 (SPAC7D4.06c) - dolichyl-P-Man:Man(5)GlcNAc(2)-PP-Dol alpha-1,3-mannosyltransferase Alg3

Gene summary

Standard name
alg3
Systematic ID
SPAC7D4.06c
Product
dolichyl-P-Man:Man(5)GlcNAc(2)-PP-Dol alpha-1,3-mannosyltransferase Alg3
Organism
Schizosaccharomyces pombe (fission yeast)
UniProt ID
Q9Y7I4
ORFeome ID
19/19D08
Characterisation status
biological role published
Feature type
mRNA gene
Genomic location
chromosome I: 2626400..2628282 forward strand

Annotation

Disease association

MONDO:0010998 - ALG3-congenital disorder of glycosylation

References:

MONDO:0015286 - congenital disorder of glycosylation

References:

MONDO:0005066 - metabolic disease

References:

GO biological process

GO:0006488 - dolichol-linked oligosaccharide biosynthetic process

References:

GO:0009101 - glycoprotein biosynthetic process

References:

GO:0006487 - protein N-linked glycosylation

References:

GO cellular component

GO:0005783 - endoplasmic reticulum

References:

GO:0098553 - lumenal side of endoplasmic reticulum membrane

References:

GO molecular function

GO:0052925 - dol-P-Man:Man(5)GlcNAc(2)-PP-Dol alpha-1,3-mannosyltransferase activity

References:

GO:0016757 - glycosyltransferase activity

References:

Multi-locus phenotype

FYPO:0008420 - abnormal dolichol-linked oligosaccharide biosynthetic process

References:

Genotypes:

FYPO:0002447 - abnormal protein N-linked glycosylation during vegetative growth

References:

Genotypes:

FYPO:0001911 - decreased protein glycosylation during vegetative growth

References:

Genotypes:

FYPO:0002061 - inviable vegetative cell population

References:

Genotypes:

FYPO:0000118 - multiseptate vegetative cell

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Genotypes:

FYPO:0001120 - pear-shaped vegetative cell

References:

Genotypes:

FYPO:0000106 - sensitive to hygromycin B

References:

Genotypes:

FYPO:0001234 - slow vegetative cell population growth

References:

Genotypes:

Protein sequence feature

SO:0000418 - signal_peptide

SO:0001812 - transmembrane_helix

References:

Qualitative gene expression

PomGeneEx:0000018 - protein level increased

References:

PomGeneEx:0000012 - RNA level decreased

References:

Quantitative gene expression

PBO:0006310 - protein level

References:

PBO:0011963 - RNA level

References:

Single locus phenotype

FYPO:0008420 - abnormal dolichol-linked oligosaccharide biosynthetic process

References:

Genotypes:

FYPO:0002447 - abnormal protein N-linked glycosylation during vegetative growth

References:

Genotypes:

FYPO:0000080 - decreased cell population growth at low temperature

References:

Genotypes:

FYPO:0009053 - decreased cell population growth on glutamate nitrogen source

References:

Genotypes:

FYPO:0009091 - decreased cell population growth on lysine and proline nitrogen source

References:

Genotypes:

FYPO:0009092 - decreased cell population growth on lysine and serine nitrogen source

References:

Genotypes:

FYPO:0009073 - decreased cell population growth on lysine nitrogen source

References:

Genotypes:

FYPO:0002924 - decreased cell population growth on maltose carbon source

References:

Genotypes:

FYPO:0007562 - decreased cell population growth on serine nitrogen source

References:

Genotypes:

FYPO:0001176 - decreased cell population growth on sucrose carbon source

References:

Genotypes:

FYPO:0000245 - loss of viability in stationary phase

References:

Genotypes:

FYPO:0002637 - normal growth on hygromycin B

References:

Genotypes:

FYPO:0001420 - normal vegetative cell population growth rate

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Genotypes:

FYPO:0001120 - pear-shaped vegetative cell

References:

Genotypes:

FYPO:0009068 - resistance to ciclopirox olamine

References:

Genotypes:

FYPO:0009034 - resistance to ethylenediaminetetraacetic acid

References:

Genotypes:

FYPO:0009081 - resistance to potassium chloride and methyl methanesulfonate

References:

Genotypes:

FYPO:0000077 - resistance to rapamycin

References:

Genotypes:

FYPO:0003383 - resistance to tert-butyl hydroperoxide

References:

Genotypes:

FYPO:0000327 - resistance to trichostatin A

References:

Genotypes:

FYPO:0007933 - sensitive to 2,2′-dipyridyl

References:

Genotypes:

FYPO:0001097 - sensitive to amitrole

References:

Genotypes:

FYPO:0000094 - sensitive to benomyl

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Genotypes:

FYPO:0000095 - sensitive to bleomycin

References:

Genotypes:

FYPO:0001501 - sensitive to brefeldin A

References:

Genotypes:

FYPO:0000096 - sensitive to cadmium

References:

Genotypes:

FYPO:0000097 - sensitive to caffeine during vegetative growth

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Genotypes:

FYPO:0000104 - sensitive to cycloheximide

References:

Genotypes:

FYPO:0007931 - sensitive to egtazic acid

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Genotypes:

FYPO:0000842 - sensitive to ethanol during vegetative growth

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Genotypes:

FYPO:0000785 - sensitive to formamide

References:

Genotypes:

FYPO:0007358 - sensitive to heavy water

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Genotypes:

FYPO:0000087 - sensitive to hydrogen peroxide

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Genotypes:

FYPO:0000088 - sensitive to hydroxyurea

References:

Genotypes:

FYPO:0009071 - sensitive to itraconazole

References:

Genotypes:

FYPO:0001719 - sensitive to lithium

References:

Genotypes:

FYPO:0009084 - sensitive to lithium chloride and methyl methanesulfonate

References:

Genotypes:

FYPO:0009086 - sensitive to lithium chloride and sodium dodecyl sulfate

References:

Genotypes:

FYPO:0006836 - sensitive to magnesium chloride

References:

Genotypes:

FYPO:0009088 - sensitive to magnesium chloride and sodium dodecyl sulfate

References:

Genotypes:

FYPO:0000089 - sensitive to methyl methanesulfonate

References:

Genotypes:

FYPO:0001214 - sensitive to potassium chloride

References:

Genotypes:

FYPO:0007924 - sensitive to potassium chloride and sodium dodecyl sulfate

References:

Genotypes:

FYPO:0000841 - sensitive to sodium dodecyl sulfate

References:

Genotypes:

FYPO:0002701 - sensitive to torin1

References:

Genotypes:

FYPO:0001457 - sensitive to tunicamycin

References:

Genotypes:

FYPO:0000115 - sensitive to valproic acid

References:

Genotypes:

FYPO:0003656 - sensitive to vanadate

References:

Genotypes:

FYPO:0002106 - viable stubby vegetative cell

References:

Genotypes:

FYPO:0001491 - viable vegetative cell

References:

Genotypes:

FYPO:0002060 - viable vegetative cell population

References:

Genotypes:

Taxonomic conservation

PBO:0011065 - conserved in eukaryotes

PBO:0011071 - conserved in eukaryotes only

PBO:0011064 - conserved in fungi

PBO:0011069 - conserved in metazoa

PBO:0011070 - conserved in vertebrates

PBO:0006222 - predominantly single copy (one to one)

Protein features

IDNameInterPro nameDB name
PF05208ALG3Glycosyltransferase_ALG3Pfam
PTHR12646Glycosyltransferase_ALG3PANTHER
Transmembrane alpha helixDeepTMHMM

Orthologs

References / Literature

PMID:9878760 - The dolichol pathway of N-linked glycosylation.
Burda P et al. Biochim Biophys Acta 1999 Jan 06;1426(2):239-57
PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.
Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623
PMID:15470240 - Schizosaccharomyces pombe Pmr1p is essential for cell wall integrity and is required for polarized cell growth and cytokinesis.
Cortés JC et al. Eukaryot Cell 2004 Oct;3(5):1124-35
PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.
Marguerat S et al. Cell 2012 Oct 26;151(3):671-83
PMID:34851357 - A novel fission yeast platform to model N-glycosylation and the bases of congenital disorders of glycosylation type I.
Gallo GL et al. J Cell Sci 2022 Mar 01;135(5)
PMID:37787768 - Broad functional profiling of fission yeast proteins using phenomics and machine learning.
Rodríguez-López M et al. Elife 2023 Oct 03;12
PMID:16823372 - ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe.
Matsuyama A et al. Nat Biotechnol 2006 Jul;24(7):841-7
PMID:31626996 - Multiplexed proteome profiling of carbon source perturbations in two yeast species with SL-SP3-TMT.
Paulo JA et al. J Proteomics 2020 Jan 06;210:103531
PB_REF:0000003 - Disease Association Curation
PMID:39367033 - Quantitative proteomics and phosphoproteomics profiling of meiotic divisions in the fission yeast Schizosaccharomyces pombe.
Sivakova B et al. Sci Rep 2024 Oct 04;14(1):23105
PMID:40015273 - A comprehensive Schizosaccharomyces pombe atlas of physical transcription factor interactions with proteins and chromatin.
Skribbe M et al. Mol Cell 2025 Feb 19;
PMID:20854854 - Production of heterologous glycoproteins by a glycosylation-defective alg3och1 mutant of Schizosaccharomyces pombe.
Ohashi T et al. J Biotechnol 2010 Nov;150(3):348-56
PMID:27168121 - Discovery of genes involved in mitosis, cell division, cell wall integrity and chromosome segregation through construction of Schizosaccharomyces pombe deletion strains.
Chen JS et al. Yeast 2016 Sep;33(9):507-17
PMID:36478272 - Translation-complex profiling of fission yeast cells reveals dynamic rearrangements of scanning ribosomal subunits upon nutritional stress.
Duncan CDS et al. Nucleic Acids Res 2022 Dec 09;50(22):13011-13025
PMID:32142608 - Mutations in a Single Signaling Pathway Allow Cell Growth in Heavy Water.
Kampmeyer C et al. ACS Synth Biol 2020 Apr 17;9(4):733-748
PMID:34250083 - Barcode sequencing and a high-throughput assay for chronological lifespan uncover ageing-associated genes in fission yeast.
Romila CA et al. Microb Cell 2021 Jul 05;8(7):146-160
PMID:11152613 - Predicting transmembrane protein topology with a hidden Markov model: application to complete genomes.
Krogh A et al. J Mol Biol 2001 Jan 19;305(3):567-80
PMID:21511999 - Comparative functional genomics of the fission yeasts.
Rhind N et al. Science 2011 May 20;332(6032):930-6
PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.
Hayles J et al. Open Biol 2013 May 22;3(5):130053
PB_REF:0000006 - Disease associations from Monarch via human-pombe orthologs