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protein coding gene - iss10 (SPAC7D4.14c) - MTREC (exosome adaptor) complex proline-rich subunit Iss10/Pir1

Gene summary

Standard name
iss10
Systematic ID
SPAC7D4.14c
Product
MTREC (exosome adaptor) complex proline-rich subunit Iss10/Pir1
Organism
Schizosaccharomyces pombe (fission yeast)
Synonyms
pir1
UniProt ID
O14269
ORFeome ID
34/34F12
Characterisation status
biological role published
Feature type
mRNA gene
Genomic location
chromosome I: 2611026..2613002 forward strand

Annotation

GO biological process

GO:0033621 - nuclear mRNA surveillance of meiosis-specific transcripts

References:

GO:1902794 - siRNA-independent facultative heterochromatin formation

References:

GO cellular component

GO:0000785 - chromatin

References:

GO:1990477 - MTREC complex

References:

GO:0016604 - nuclear body

References:

GO:1990251 - nuclear exosome focus

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GO molecular function

GO:0005515 - protein binding

References:

Modification

MOD:00006 - N-glycosylated residue

References:

MOD:00046 - O-phospho-L-serine

References:

MOD:00047 - O-phospho-L-threonine

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MOD:00696 - phosphorylated residue

References:

MOD:01149 - sumoylated lysine

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MOD:01148 - ubiquitinylated lysine

References:

Multi-locus phenotype

FYPO:0002768 - decreased protein ubiquitination during vegetative growth

References:

Genotypes:

FYPO:0003234 - normal histone H3-K9 methylation at heterochromatin domain during vegetative growth

References:

Genotypes:

FYPO:0000833 - normal protein level during vegetative growth

References:

Genotypes:

Qualitative gene expression

PomGeneEx:0000019 - protein level decreased

References:

PomGeneEx:0000021 - protein present

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PomGeneEx:0000013 - RNA level unchanged

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PomGeneEx:0000014 - RNA present

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Quantitative gene expression

PBO:0006310 - protein level

References:

PBO:0011963 - RNA level

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Single locus phenotype

FYPO:0004159 - abnormal homologous chromosome segregation

References:

Genotypes:

FYPO:0003748 - abnormal protein localization to nuclear body

References:

Genotypes:

FYPO:0003066 - abnormal sporulation resulting in formation of ascus with fewer than four spores

References:

Genotypes:

FYPO:0003042 - abolished protein localization to nuclear exosome focus

References:

Genotypes:

FYPO:0002033 - abolished protein phosphorylation during vegetative growth

References:

Genotypes:

FYPO:0000080 - decreased cell population growth at low temperature

References:

Genotypes:

FYPO:0003743 - decreased cell population growth during glucose starvation

References:

Genotypes:

FYPO:0003230 - decreased histone H3-K9 methylation at heterochromatin island during vegetative growth

References:

Genotypes:

FYPO:0005121 - decreased level of early meiotic gene mRNA during meiotic cell cycle

References:

Genotypes:

FYPO:0000485 - decreased meiotic recombination

References:

Genotypes:

FYPO:0001645 - decreased protein-protein interaction

References:

Genotypes:

FYPO:0000581 - decreased spore germination frequency

References:

Genotypes:

FYPO:0001355 - decreased vegetative cell population growth

References:

Genotypes:

FYPO:0003557 - increased antisense RNA level

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Genotypes:

FYPO:0009052 - increased cell population growth on glutamate nitrogen source

References:

Genotypes:

FYPO:0009094 - increased cell population growth on lysine and proline nitrogen source

References:

Genotypes:

FYPO:0004966 - increased duration of horsetail movement

References:

Genotypes:

FYPO:0002960 - increased level of DSR-containing meiotic gene mRNA during vegetative growth

References:

Genotypes:

FYPO:0002173 - increased level of meiotic gene mRNA during vegetative growth

References:

Genotypes:

FYPO:0007686 - increased number of nuclear exosome foci

References:

Genotypes:

FYPO:0003043 - increased protein localization to Mei2 nuclear dot

References:

Genotypes:

FYPO:0007685 - increased vegetative cell population growth during nutrient starvation

References:

Genotypes:

FYPO:0006518 - loss of viability in G0

References:

Genotypes:

FYPO:0000245 - loss of viability in stationary phase

References:

Genotypes:

FYPO:0000674 - normal cell population growth at high temperature

References:

Genotypes:

FYPO:0002567 - normal centromeric outer repeat transcript level

References:

Genotypes:

FYPO:0007553 - normal G1 to G0 transition

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Genotypes:

FYPO:0001164 - normal growth on glucose carbon source

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Genotypes:

FYPO:0003235 - normal histone H3-K9 methylation at centromere outer repeat during vegetative growth

References:

Genotypes:

FYPO:0000478 - normal meiosis

References:

Genotypes:

FYPO:0007687 - normal protein localization to nuclear exosome focus during meiosis

References:

Genotypes:

FYPO:0002052 - normal sporulation frequency

References:

Genotypes:

FYPO:0001357 - normal vegetative cell population growth

References:

Genotypes:

FYPO:0000067 - resistance to brefeldin A

References:

Genotypes:

FYPO:0009038 - resistance to egtazic acid

References:

Genotypes:

FYPO:0001583 - resistance to lithium

References:

Genotypes:

FYPO:0009087 - resistance to magnesium chloride and sodium dodecyl sulfate

References:

Genotypes:

FYPO:0009043 - resistance to potassium chloride and sodium dodecyl sulfate

References:

Genotypes:

FYPO:0001034 - resistance to tunicamycin

References:

Genotypes:

FYPO:0007921 - sensitive to benzamidine

References:

Genotypes:

FYPO:0000097 - sensitive to caffeine during vegetative growth

References:

Genotypes:

FYPO:0000785 - sensitive to formamide

References:

Genotypes:

FYPO:0009088 - sensitive to magnesium chloride and sodium dodecyl sulfate

References:

Genotypes:

FYPO:0002328 - sensitive to terbinafine

References:

Genotypes:

FYPO:0003656 - sensitive to vanadate

References:

Genotypes:

FYPO:0009064 - sensitive to X-rays and rapamycin during vegetative growth.

References:

Genotypes:

FYPO:0002060 - viable vegetative cell population

References:

Genotypes:

FYPO:0002177 - viable vegetative cell with normal cell morphology

References:

Genotypes:

Taxonomic conservation

PBO:0016885 - Schizosaccharomyces specific

Protein features

IDNameInterPro nameDB name
mobidb-lite-Disorderdisorder_predictionMOBIDB-Disorder
mobidb-lite-Low-complexitydisorder_predictionMOBIDB-Low-complexity
mobidb-lite-Polardisorder_predictionMOBIDB-Polar

References / Literature

PMID:23980030 - A novel factor Iss10 regulates Mmi1-mediated selective elimination of meiotic transcripts.
Yamashita A et al. Nucleic Acids Res 2013 Nov;41(21):9680-7
PMID:30726745 - Fission Yeast NDR/LATS Kinase Orb6 Regulates Exocytosis via Phosphorylation of the Exocyst Complex.
Tay YD et al. Cell Rep 2019 Feb 05;26(6):1654-1667.e7
PMID:19547744 - Evolution of phosphoregulation: comparison of phosphorylation patterns across yeast species.
Beltrao P et al. PLoS Biol 2009 Jun 16;7(6):e1000134
PMID:30321377 - Proteomic profiling and functional characterization of post-translational modifications of the fission yeast RNA exosome.
Telekawa C et al. Nucleic Acids Res 2018 Nov 30;46(21):11169-11183
PMID:25720772 - Quantitative phosphoproteomics reveals pathways for coordination of cell growth and division by the conserved fission yeast kinase pom1.
Kettenbach AN et al. Mol Cell Proteomics 2015 May;14(5):1275-87
PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.
Marguerat S et al. Cell 2012 Oct 26;151(3):671-83
PMID:34250083 - Barcode sequencing and a high-throughput assay for chronological lifespan uncover ageing-associated genes in fission yeast.
Romila CA et al. Microb Cell 2021 Jul 05;8(7):146-160
PMID:26537787 - Targeting of SUMO substrates to a Cdc48-Ufd1-Npl4 segregase and STUbL pathway in fission yeast.
Køhler JB et al. Nat Commun 2015 Nov 05;6:8827
PMID:24713849 - Post-transcriptional regulation of meiotic genes by a nuclear RNA silencing complex.
Egan ED et al. RNA 2014 Jun;20(6):867-81
PMID:39367033 - Quantitative proteomics and phosphoproteomics profiling of meiotic divisions in the fission yeast Schizosaccharomyces pombe.
Sivakova B et al. Sci Rep 2024 Oct 04;14(1):23105
PMID:39705284 - Proteomic and phosphoproteomic analyses reveal that TORC1 is reactivated by pheromone signaling during sexual reproduction in fission yeast.
Bérard M et al. PLoS Biol 2024 Dec 20;22(12):e3002963
PMID:24763107 - Absolute proteome and phosphoproteome dynamics during the cell cycle of Schizosaccharomyces pombe (Fission Yeast).
Carpy A et al. Mol Cell Proteomics 2014 Aug;13(8):1925-36
PMID:27984725 - CDK Substrate Phosphorylation and Ordering the Cell Cycle.
Swaffer MP et al. Cell 2016 Dec 15;167(7):1750-1761.e16
PMID:22633491 - Mapping N-glycosylation sites across seven evolutionarily distant species reveals a divergent substrate proteome despite a common core machinery.
Zielinska DF et al. Mol Cell 2012 May 25;46(4):542-8
PMID:25989903 - The fission yeast MTREC complex targets CUTs and unspliced pre-mRNAs to the nuclear exosome.
Zhou Y et al. Nat Commun 2015 May 20;6:7050
PMID:33574613 - TOR targets an RNA processing network to regulate facultative heterochromatin, developmental gene expression and cell proliferation.
Wei Y et al. Nat Cell Biol 2021 Mar;23(3):243-256
PMID:24210919 - Mtr4-like protein coordinates nuclear RNA processing for heterochromatin assembly and for telomere maintenance.
Lee NN et al. Cell 2013 Nov 21;155(5):1061-74
PMID:22540037 - Predicting the fission yeast protein interaction network.
Pancaldi V et al. G3 (Bethesda) 2012 Apr;2(4):453-67
PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.
Hayles J et al. Open Biol 2013 May 22;3(5):130053
PMID:32012158 - Meiotic gene silencing complex MTREC/NURS recruits the nuclear exosome to YTH-RNA-binding protein Mmi1.
Shichino Y et al. PLoS Genet 2020 Feb;16(2):e1008598
PMID:33109728 - High-Throughput Identification of Nuclear Envelope Protein Interactions in Schizosaccharomyces pombe Using an Arrayed Membrane Yeast-Two Hybrid Library.
Varberg JM et al. G3 (Bethesda) 2020 Dec 03;10(12):4649-4663
PMID:39476757 - Characterization of Ksg1 protein kinase-dependent phosphoproteome in the fission yeast S. pombe.
Cipak L et al. Biochem Biophys Res Commun 2024 Oct 25;736:150895
PMID:33823663 - A TOR (target of rapamycin) and nutritional phosphoproteome of fission yeast reveals novel targets in networks conserved in humans.
Halova L et al. Open Biol 2021 Apr;11(4):200405
PMID:33260998 - High-Throughput Flow Cytometry Combined with Genetic Analysis Brings New Insights into the Understanding of Chromatin Regulation of Cellular Quiescence.
Zahedi Y et al. Int J Mol Sci 2020 Nov 27;21(23)
PMID:37787768 - Broad functional profiling of fission yeast proteins using phenomics and machine learning.
Rodríguez-López M et al. Elife 2023 Oct 03;12
PMID:21712547 - Mitotic substrates of the kinase aurora with roles in chromatin regulation identified through quantitative phosphoproteomics of fission yeast.
Koch A et al. Sci Signal 2011 Jun 28;4(179):rs6
PMID:36002457 - Structural analysis of Red1 as a conserved scaffold of the RNA-targeting MTREC/PAXT complex.
Foucher AE et al. Nat Commun 2022 Aug 24;13(1):4969
PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.
Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623
PMID:26404184 - High Confidence Fission Yeast SUMO Conjugates Identified by Tandem Denaturing Affinity Purification.
Nie M et al. Sci Rep 2015 Sep 25;5:14389
PMID:31064814 - Proximity-dependent biotinylation mediated by TurboID to identify protein-protein interaction networks in yeast.
Larochelle M et al. J Cell Sci 2019 May 31;132(11)
PMID:26942678 - Enhancer of Rudimentary Cooperates with Conserved RNA-Processing Factors to Promote Meiotic mRNA Decay and Facultative Heterochromatin Assembly.
Sugiyama T et al. Mol Cell 2016 Mar 03;61(5):747-759
PMID:29996109 - Quantitative Phosphoproteomics Reveals the Signaling Dynamics of Cell-Cycle Kinases in the Fission Yeast Schizosaccharomyces pombe.
Swaffer MP et al. Cell Rep 2018 Jul 10;24(2):503-514
PMID:28841135 - Ubiquitination-dependent control of sexual differentiation in fission yeast.
Simonetti F et al. Elife 2017 Aug 25;6
PMID:26771498 - A Proteome-wide Fission Yeast Interactome Reveals Network Evolution Principles from Yeasts to Human.
Vo TV et al. Cell 2016 Jan 14;164(1-2):310-323