protein coding gene - slp1 (SPAC821.08c) - substrate-specific mitotic metaphase/anaphase APC coactivator Slp1/Cdc20

Gene summary

Standard name

slp1

Systematic ID

SPAC821.08c

Product

substrate-specific mitotic metaphase/anaphase APC coactivator Slp1/Cdc20

Organism

Schizosaccharomyces pombe (fission yeast)

Synonyms

cdc20

UniProt ID

P78972

ORFeome ID

23/23F01

Characterisation status

biological role published

Feature type

mRNA gene

Genomic location

chromosome I: 994941..997635 reverse strand

Annotation

Protein features

Orthologs

• CDC20 (HGNC:1723) Homo sapiens
• slp1 (SJAG_00666) Schizosaccharomyces japonicus
• CDC20 (YGL116W) Saccharomyces cerevisiae

References / Literature

PMID:15509866 - Fkh2p and Sep1p regulate mitotic gene transcription in fission yeast.

Buck V et al. J Cell Sci 2004 Nov 01;117(Pt 23):5623-32

PMID:15930132 - Spindle checkpoint signaling requires the mis6 kinetochore subcomplex, which interacts with mad2 and mitotic spindles.

Saitoh S et al. Mol Biol Cell 2005 Aug;16(8):3666-77

GO_REF:0000051 - S. pombe keyword mapping

PMID:26527280 - Robust Ordering of Anaphase Events by Adaptive Thresholds and Competing Degradation Pathways.

Kamenz J et al. Mol Cell 2015 Nov 05;60(3):446-59

PMID:36108046 - Recovery from spindle checkpoint-mediated arrest requires a novel Dnt1-dependent APC/C activation mechanism.

Bai S et al. PLoS Genet 2022 Sep;18(9):e1010397

PMID:24583014 - Slow checkpoint activation kinetics as a safety device in anaphase.

Kamenz J et al. Curr Biol 2014 Mar 17;24(6):646-51

PMID:33823663 - A TOR (target of rapamycin) and nutritional phosphoproteome of fission yeast reveals novel targets in networks conserved in humans.

Halova L et al. Open Biol 2021 Apr;11(4):200405

PMID:9461438 - Fission yeast Slp1: an effector of the Mad2-dependent spindle checkpoint.

Kim SH et al. Science 1998 Feb 13;279(5353):1045-7

PMID:16823372 - ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe.

Matsuyama A et al. Nat Biotechnol 2006 Jul;24(7):841-7

PMID:11084332 - Cut8, essential for anaphase, controls localization of 26S proteasome, facilitating destruction of cyclin and Cut2.

Tatebe H et al. Curr Biol 2000 Nov 02;10(21):1329-38

PMID:26882497 - Mps1Mph1 Kinase Phosphorylates Mad3 to Inhibit Cdc20Slp1-APC/C and Maintain Spindle Checkpoint Arrests.

Zich J et al. PLoS Genet 2016 Feb;12(2):e1005834

PMID:28245054 - Fission yeast APC/C activators Slp1 and Fzr1 sequentially trigger two consecutive nuclear divisions during meiosis.

Chikashige Y et al. FEBS Lett 2017 Apr;591(7):1029-1040

PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.

Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623

PMID:21389117 - Mes1 controls the meiosis I to meiosis II transition by distinctly regulating the anaphase-promoting complex/cyclosome coactivators Fzr1/Mfr1 and Slp1 in fission yeast.

Kimata Y et al. Mol Biol Cell 2011 May;22(9):1486-94

PMID:21511999 - Comparative functional genomics of the fission yeasts.

Rhind N et al. Science 2011 May 20;332(6032):930-6

PMID:18331722 - A mutual inhibition between APC/C and its substrate Mes1 required for meiotic progression in fission yeast.

Kimata Y et al. Dev Cell 2008 Mar;14(3):446-54

PMID:22681890 - Hierarchical modularity and the evolution of genetic interactomes across species.

Ryan CJ et al. Mol Cell 2012 Jun 08;46(5):691-704

PMID:21712547 - Mitotic substrates of the kinase aurora with roles in chromatin regulation identified through quantitative phosphoproteomics of fission yeast.

Koch A et al. Sci Signal 2011 Jun 28;4(179):rs6

PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.

Marguerat S et al. Cell 2012 Oct 26;151(3):671-83

PMID:23442800 - An E2 enzyme Ubc11 is required for ubiquitination of Slp1/Cdc20 and spindle checkpoint silencing in fission yeast.

Horikoshi Y et al. Cell Cycle 2013 Mar 15;12(6):961-71

PMID:29996109 - Quantitative Phosphoproteomics Reveals the Signaling Dynamics of Cell-Cycle Kinases in the Fission Yeast Schizosaccharomyces pombe.

Swaffer MP et al. Cell Rep 2018 Jul 10;24(2):503-514

PMID:33313903 - Ribosome profiling reveals ribosome stalling on tryptophan codons and ribosome queuing upon oxidative stress in fission yeast.

Rubio A et al. Nucleic Acids Res 2021 Jan 11;49(1):383-399

PMID:39367033 - Quantitative proteomics and phosphoproteomics profiling of meiotic divisions in the fission yeast Schizosaccharomyces pombe.

Sivakova B et al. Sci Rep 2024 Oct 04;14(1):23105

PMID:9398669 - A WD repeat protein controls the cell cycle and differentiation by negatively regulating Cdc2/B-type cyclin complexes.

Yamaguchi S et al. Mol Biol Cell 1997 Dec;8(12):2475-86

PMID:10582241 - Control of metaphase-anaphase progression by proteolysis: cyclosome function regulated by the protein kinase A pathway, ubiquitination and localization.

Yanagida M et al. Philos Trans R Soc Lond B Biol Sci 1999 Sep 29;354(1389):1559-69; discussion 1569-70

PMID:25720772 - Quantitative phosphoproteomics reveals pathways for coordination of cell growth and division by the conserved fission yeast kinase pom1.

Kettenbach AN et al. Mol Cell Proteomics 2015 May;14(5):1275-87

PMID:28178520 - Identification of a Sgo2-Dependent but Mad2-Independent Pathway Controlling Anaphase Onset in Fission Yeast.

Meadows JC et al. Cell Rep 2017 Feb 07;18(6):1422-1433

GO_REF:0000033 - Annotation inferences using phylogenetic trees

PMID:12653962 - A brute force postgenome approach to identify temperature-sensitive mutations that negatively interact with separase and securin plasmids.

Matsumura T et al. Genes Cells 2003 Apr;8(4):341-55

PMID:11950879 - Control of localization of a spindle checkpoint protein, Mad2, in fission yeast.

Ikui AE et al. J Cell Sci 2002 Apr 15;115(Pt 8):1603-10

PMID:24763107 - Absolute proteome and phosphoproteome dynamics during the cell cycle of Schizosaccharomyces pombe (Fission Yeast).

Carpy A et al. Mol Cell Proteomics 2014 Aug;13(8):1925-36

PMID:24948786 - Histone H2B ubiquitination promotes the function of the anaphase-promoting complex/cyclosome in Schizosaccharomyces pombe.

Elmore ZC et al. G3 (Bethesda) 2014 Jun 19;4(8):1529-38

PB_REF:0000006 - Disease associations from Monarch via human-pombe orthologs

PMID:27664031 - C-terminal region of Mad2 plays an important role during mitotic spindle checkpoint in fission yeast Schizosaccharomyces pombe.

Singh GK et al. Mol Biol Rep 2017 Feb;44(1):89-96

PMID:11058086 - High dosage expression of a zinc finger protein, Grt1, suppresses a mutant of fission yeast slp1(+), a homolog of CDC20/p55CDC/Fizzy.

Yamada HY et al. J Cell Sci 2000 Nov;113 ( Pt 22):3989-99

PMID:22437499 - Structure of the mitotic checkpoint complex.

Chao WC et al. Nature 2012 Mar 21;484(7393):208-13

PMID:11909965 - Fission yeast Mad3p is required for Mad2p to inhibit the anaphase-promoting complex and localizes to kinetochores in a Bub1p-, Bub3p-, and Mph1p-dependent manner.

Millband DN et al. Mol Cell Biol 2002 Apr;22(8):2728-42

PMID:15791259 - Fission yeast Mes1p ensures the onset of meiosis II by blocking degradation of cyclin Cdc13p.

Izawa D et al. Nature 2005 Mar 24;434(7032):529-33

PMID:18556659 - The spindle checkpoint functions of Mad3 and Mad2 depend on a Mad3 KEN box-mediated interaction with Cdc20-anaphase-promoting complex (APC/C).

Sczaniecka M et al. J Biol Chem 2008 Aug 22;283(34):23039-47

PMID:15347659 - DNA replication checkpoint control mediated by the spindle checkpoint protein Mad2p in fission yeast.

Sugimoto I et al. J Biol Chem 2004 Nov 05;279(45):47372-8

PMID:22184248 - Centromere-tethered Mps1 pombe homolog (Mph1) kinase is a sufficient marker for recruitment of the spindle checkpoint protein Bub1, but not Mad1.

Ito D et al. Proc Natl Acad Sci U S A 2012 Jan 03;109(1):209-14

PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.

Hayles J et al. Open Biol 2013 May 22;3(5):130053

PMID:27984744 - Survival in Quiescence Requires the Euchromatic Deployment of Clr4/SUV39H by Argonaute-Associated Small RNAs.

Joh RI et al. Mol Cell 2016 Dec 15;64(6):1088-1101

PMID:28366743 - Different Functionality of Cdc20 Binding Sites within the Mitotic Checkpoint Complex.

Sewart K et al. Curr Biol 2017 Apr 24;27(8):1213-1220

PMID:28366744 - Fission Yeast Apc15 Stabilizes MCC-Cdc20-APC/C Complexes, Ensuring Efficient Cdc20 Ubiquitination and Checkpoint Arrest.

May KM et al. Curr Biol 2017 Apr 24;27(8):1221-1228

PMID:30726745 - Fission Yeast NDR/LATS Kinase Orb6 Regulates Exocytosis via Phosphorylation of the Exocyst Complex.

Tay YD et al. Cell Rep 2019 Feb 05;26(6):1654-1667.e7

PMID:32062975 - Phosphoproteomics Reveals Novel Targets and Phosphoprotein Networks in Cell Cycle Mediated by Dsk1 Kinase.

Wu M et al. J Proteome Res 2020 Apr 03;19(4):1776-1787

PMID:37970674 - SUMOylation regulates Lem2 function in centromere clustering and silencing.

Strachan J et al. J Cell Sci 2023 Dec 01;136(23)

PMID:9001228 - A fission yeast homolog of CDC20/p55CDC/Fizzy is required for recovery from DNA damage and genetically interacts with p34cdc2.

Matsumoto T Mol Cell Biol 1997 Feb;17(2):742-50

PMID:40015273 - A comprehensive Schizosaccharomyces pombe atlas of physical transcription factor interactions with proteins and chromatin.

Skribbe M et al. Mol Cell 2025 Feb 19;

PMID:22907753 - Posttranscriptional regulation of cell-cell interaction protein-encoding transcripts by Zfs1p in Schizosaccharomyces pombe.

Wells ML et al. Mol Cell Biol 2012 Oct;32(20):4206-14

PMID:25847133 - RNA pol II transcript abundance controls condensin accumulation at mitotically up-regulated and heat-shock-inducible genes in fission yeast.

Nakazawa N et al. Genes Cells 2015 Jun;20(6):481-99

PMID:29084823 - Phosphorylation of the RNA-binding protein Zfs1 modulates sexual differentiation in fission yeast.

Navarro FJ et al. J Cell Sci 2017 Dec 15;130(24):4144-4154

PMID:30759079 - A systematic genetic screen identifies essential factors involved in nuclear size control.

Cantwell H et al. PLoS Genet 2019 Feb;15(2):e1007929

PMID:24161933 - Determinants of robustness in spindle assembly checkpoint signalling.

Heinrich S et al. Nat Cell Biol 2013 Nov;15(11):1328-39