protein coding gene - orb6 (SPAC821.12) - serine/threonine protein kinase Orb6

Gene summary

Standard name

orb6

Systematic ID

SPAC821.12

Product

serine/threonine protein kinase Orb6

Organism

Schizosaccharomyces pombe (fission yeast)

UniProt ID

O13310

ORFeome ID

45/45F06

Characterisation status

biological role published

Feature type

mRNA gene

Genomic location

chromosome I: 1008263..1010318 forward strand

Annotation

Protein features

Orthologs

• STK38 (HGNC:17847) Homo sapiens
• STK38L (HGNC:17848) Homo sapiens
• orb6 (SJAG_00661) Schizosaccharomyces japonicus
• CBK1 (YNL161W) Saccharomyces cerevisiae

References / Literature

PMID:8522609 - Fission yeast cell morphogenesis: identification of new genes and analysis of their role during the cell cycle.

Verde F et al. J Cell Biol 1995 Dec;131(6 Pt 1):1529-38

PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.

Hayles J et al. Open Biol 2013 May 22;3(5):130053

PMID:12529446 - Gef1p, a new guanine nucleotide exchange factor for Cdc42p, regulates polarity in Schizosaccharomyces pombe.

Coll PM et al. Mol Biol Cell 2003 Jan;14(1):313-23

PMID:12646585 - Control of cell polarity in fission yeast by association of Orb6p kinase with the highly conserved protein methyltransferase Skb1p.

Wiley DJ et al. J Biol Chem 2003 Jul 04;278(27):25256-63

PMID:38442865 - The fission yeast NDR kinase Orb6 and its signalling pathway MOR regulate cytoplasmic microtubule organization during the cell cycle.

Kume K et al. Open Biol 2024 Mar;14(3):230440

PMID:12456722 - Mob2p interacts with the protein kinase Orb6p to promote coordination of cell polarity with cell cycle progression.

Hou MC et al. J Cell Sci 2003 Jan 01;116(Pt 1):125-35

PMID:27474797 - Spatial control of translation repression and polarized growth by conserved NDR kinase Orb6 and RNA-binding protein Sts5.

Nuñez I et al. Elife 2016 Jul 30;5

PMID:20805322 - The mitosis-to-interphase transition is coordinated by cross talk between the SIN and MOR pathways in Schizosaccharomyces pombe.

Ray S et al. J Cell Biol 2010 Sep 06;190(5):793-805

PMID:12234926 - Fission yeast Mor2/Cps12, a protein similar to Drosophila Furry, is essential for cell morphogenesis and its mutation induces Wee1-dependent G(2) delay.

Hirata D et al. EMBO J 2002 Sep 16;21(18):4863-74

PMID:16096637 - Fission yeast MO25 protein is localized at SPB and septum and is essential for cell morphogenesis.

Kanai M et al. EMBO J 2005 Sep 07;24(17):3012-25

PMID:23615447 - Compartmentalized nodes control mitotic entry signaling in fission yeast.

Deng L et al. Mol Biol Cell 2013 Jun;24(12):1872-81

PMID:34296454 - The TOR-dependent phosphoproteome and regulation of cellular protein synthesis.

Mak T et al. EMBO J 2021 Aug 16;40(16):e107911

PMID:9636183 - Fission yeast orb6, a ser/thr protein kinase related to mammalian rho kinase and myotonic dystrophy kinase, is required for maintenance of cell polarity and coordinates cell morphogenesis with the cell cycle.

Verde F et al. Proc Natl Acad Sci U S A 1998 Jun 23;95(13):7526-31

PMID:27852900 - Gradients of phosphatidylserine contribute to plasma membrane charge localization and cell polarity in fission yeast.

Haupt A et al. Mol Biol Cell 2017 Jan 01;28(1):210-220

PMID:17377067 - Regulation of cell diameter, For3p localization, and cell symmetry by fission yeast Rho-GAP Rga4p.

Das M et al. Mol Biol Cell 2007 Jun;18(6):2090-101

PMID:16325501 - The fission yeast MO25 protein functions in polar growth and cell separation.

Mendoza M et al. Eur J Cell Biol 2005 Dec;84(12):915-26

PMID:39367033 - Quantitative proteomics and phosphoproteomics profiling of meiotic divisions in the fission yeast Schizosaccharomyces pombe.

Sivakova B et al. Sci Rep 2024 Oct 04;14(1):23105

PMID:20826805 - Fission yeast germinal center (GC) kinase Ppk11 interacts with Pmo25 and plays an auxiliary role in concert with the morphogenesis Orb6 network (MOR) in cell morphogenesis.

Goshima T et al. J Biol Chem 2010 Nov 05;285(45):35196-205

PMID:23051734 - Myosin Vs organize actin cables in fission yeast.

Lo Presti L et al. Mol Biol Cell 2012 Dec;23(23):4579-91

PMID:25422470 - Oscillatory AAA+ ATPase Knk1 constitutes a novel morphogenetic pathway in fission yeast.

Scheffler K et al. Proc Natl Acad Sci U S A 2014 Dec 16;111(50):17899-904

PMID:29290560 - ER-PM Contacts Restrict Exocytic Sites for Polarized Morphogenesis.

Ng AYE et al. Curr Biol 2018 Jan 08;28(1):146-153.e5

PMID:16823372 - ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe.

Matsuyama A et al. Nat Biotechnol 2006 Jul;24(7):841-7

PMID:17317928 - A method for Pmo25-associated kinase assay in fission yeast: the activity is dependent on two gC kinases Nak1 and Sid1.

Kume K et al. Biosci Biotechnol Biochem 2007 Feb;71(2):615-7

PMID:30726745 - Fission Yeast NDR/LATS Kinase Orb6 Regulates Exocytosis via Phosphorylation of the Exocyst Complex.

Tay YD et al. Cell Rep 2019 Feb 05;26(6):1654-1667.e7

PMID:34282727 - Pak1 kinase controls cell shape through ribonucleoprotein granules.

Magliozzi JO et al. Elife 2021 Jul 20;10

PMID:22629372 - Conserved Orb6 phosphorylation sites are essential for polarized cell growth in Schizosaccharomyces pombe.

Liu G et al. PLoS One 2012;7(5):e37221

PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.

Marguerat S et al. Cell 2012 Oct 26;151(3):671-83

PMID:29996109 - Quantitative Phosphoproteomics Reveals the Signaling Dynamics of Cell-Cycle Kinases in the Fission Yeast Schizosaccharomyces pombe.

Swaffer MP et al. Cell Rep 2018 Jul 10;24(2):503-514

PMID:34666001 - Detection of surface forces by the cell-wall mechanosensor Wsc1 in yeast.

Neeli-Venkata R et al. Dev Cell 2021 Oct 25;56(20):2856-2870.e7

PMID:35099006 - Fission yeast polycystin Pkd2p promotes cell size expansion and antagonizes the Hippo-related SIN pathway.

Sinha D et al. J Cell Sci 2022 Feb 15;135(4)

PMID:23649273 - Fission yeast leucine-rich repeat protein Lrp1 is essential for cell morphogenesis as a component of the morphogenesis Orb6 network (MOR).

Kume K et al. Biosci Biotechnol Biochem 2013;77(5):1086-91

PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.

Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623

PMID:19646873 - The conserved NDR kinase Orb6 controls polarized cell growth by spatial regulation of the small GTPase Cdc42.

Das M et al. Curr Biol 2009 Aug 11;19(15):1314-9

PMID:17998401 - Nuclear size control in fission yeast.

Neumann FR et al. J Cell Biol 2007 Nov 19;179(4):593-600

PMID:24972934 - Cross talk between NDR kinase pathways coordinates cytokinesis with cell separation in Schizosaccharomyces pombe.

Gupta S et al. Eukaryot Cell 2014 Aug;13(8):1104-12

PMID:26246599 - Phosphorylation-dependent inhibition of Cdc42 GEF Gef1 by 14-3-3 protein Rad24 spatially regulates Cdc42 GTPase activity and oscillatory dynamics during cell morphogenesis.

Das M et al. Mol Biol Cell 2015 Oct 01;26(19):3520-34

PMID:36200871 - Membrane stretching activates calcium permeability of a putative channel Pkd2 during fission yeast cytokinesis.

Poddar A et al. Mol Biol Cell 2022 Dec 01;33(14):ar134

PMID:21511999 - Comparative functional genomics of the fission yeasts.

Rhind N et al. Science 2011 May 20;332(6032):930-6

PMID:19474789 - A spatial gradient coordinates cell size and mitotic entry in fission yeast.

Moseley JB et al. Nature 2009 Jun 11;459(7248):857-60

PMID:37970674 - SUMOylation regulates Lem2 function in centromere clustering and silencing.

Strachan J et al. J Cell Sci 2023 Dec 01;136(23)

PMID:29689193 - Mechanosensation Dynamically Coordinates Polar Growth and Cell Wall Assembly to Promote Cell Survival.

Davì V et al. Dev Cell 2018 Apr 23;45(2):170-182.e7

PMID:24763107 - Absolute proteome and phosphoproteome dynamics during the cell cycle of Schizosaccharomyces pombe (Fission Yeast).

Carpy A et al. Mol Cell Proteomics 2014 Aug;13(8):1925-36

PMID:18495844 - Interphase microtubule bundles use global cell shape to guide spindle alignment in fission yeast.

Daga RR et al. J Cell Sci 2008 Jun 15;121(Pt 12):1973-80

PMID:40931936 - Oligomerization and exocyst coupling underlie Spa2-mediated focusing of polarized growth in fission yeast.

Ren L et al. J Cell Sci 2025 Sep 01;138(17)

PMID:42017229 - Temperature-specific regulation of the NDR kinase Orb6 by the MAPK Sty1 to promote heat stress resilience.

Doyle LP et al. J Cell Sci 2026 Apr 15;139(8)