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protein coding gene - dnf1 (SPAC821.13c) - trans-Golgi network aminophospholipid translocase (flippase), Dnf1

Gene summary

Standard name
dnf1
Systematic ID
SPAC821.13c
Product
trans-Golgi network aminophospholipid translocase (flippase), Dnf1
Organism
Schizosaccharomyces pombe (fission yeast)
Synonyms
SPAC955.01c
UniProt ID
Q9UT43
ORFeome ID
48/48G07
Characterisation status
biological role inferred
Feature type
mRNA gene
Genomic location
chromosome I: 1010343..1015362 reverse strand

Annotation

GO biological process

GO:0032456 - endocytic recycling

References:

GO:0045332 - phospholipid translocation

References:

GO:0006892 - post-Golgi vesicle-mediated transport

References:

GO cellular component

GO:0000935 - division septum

References:

GO:0010008 - endosome membrane

References:

GO:0000139 - Golgi membrane

References:

GO:0005886 - plasma membrane

References:

GO:0005802 - trans-Golgi network

References:

GO:0030140 - trans-Golgi network transport vesicle

References:

GO molecular function

GO:0005524 - ATP binding

References:

GO:0016887 - ATP hydrolysis activity

References:

GO:0000287 - magnesium ion binding

References:

GO:0090554 - phosphatidylcholine floppase activity

References:

Modification

MOD:00046 - O-phospho-L-serine

References:

MOD:00047 - O-phospho-L-threonine

References:

MOD:01148 - ubiquitinylated lysine

References:

Multi-locus phenotype

FYPO:0001357 - normal vegetative cell population growth

References:

Genotypes:

Protein sequence feature

SO:0001812 - transmembrane_helix

References:

Quantitative gene expression

PBO:0006310 - protein level

References:

PBO:0011963 - RNA level

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Single locus phenotype

FYPO:0000135 - abnormal plasma membrane sterol distribution

References:

Genotypes:

FYPO:0002060 - viable vegetative cell population

References:

Genotypes:

FYPO:0002177 - viable vegetative cell with normal cell morphology

References:

Genotypes:

Taxonomic conservation

PBO:0011065 - conserved in eukaryotes

PBO:0011071 - conserved in eukaryotes only

PBO:0011064 - conserved in fungi

PBO:0011069 - conserved in metazoa

Protein features

IDNameInterPro nameDB name
PF13246Cation_ATPasePfam
PF16209PhoLip_ATPase_NP-type_ATPase_NPfam
PF16212PhoLip_ATPase_CP_typ_ATPase_cPfam
cd02073P-type_ATPase_APLT_Dnf-likeCDD
PS00154ATPASE_E1_E2ATPase_P-typ_P_sitePROSITE patterns
PR00119CATATPASEPRINTS
G3DSA:3.40.50.1000:FF:000014CATH-FunFam
G3DSA:2.70.150.10CATH-Gene3D
G3DSA:3.40.1110.10ATPase_P-typ_cyto_dom_NCATH-Gene3D
G3DSA:3.40.50.1000HAD_sfCATH-Gene3D
SSF56784HAD-like_sfSUPERFAMILY
SSF81653ATPase_P-typ_transduc_dom_A_sfSUPERFAMILY
SSF81660ATPase_P-typ_cyto_dom_NSUPERFAMILY
SSF81665ATPase_P-typ_TM_dom_sfSUPERFAMILY
PTHR24092PANTHER
TIGR01494ATPase_P-typeP_typ_ATPaseNCBIFAM
TIGR01652ATPase-PlipidP-type_ATPase_IVNCBIFAM
SFLDF00027p-type_atpaseP_typ_ATPase_HD_domSFLD
SFLDG00002C1.7:_P-type_atpase_likeSFLD
SFLDS00003Haloacid_DehalogenaseSFLD
Transmembrane alpha helixDeepTMHMM
mobidb-lite-Disorderdisorder_predictionMobiDB-Disorder
mobidb-lite-Negative-Polyelectrolytedisorder_predictionMobiDB-Negative-Polyelectrolyte
mobidb-lite-Polardisorder_predictionMobiDB-Polar
mobidb-lite-Polyampholytedisorder_predictionMobiDB-Polyampholyte
mobidb-lite-Proline-richdisorder_predictionMobiDB-Proline-rich

Orthologs

References / Literature

PMID:39705284 - Proteomic and phosphoproteomic analyses reveal that TORC1 is reactivated by pheromone signaling during sexual reproduction in fission yeast.
Bérard M et al. PLoS Biol 2024 Dec 20;22(12):e3002963
PMID:11152613 - Predicting transmembrane protein topology with a hidden Markov model: application to complete genomes.
Krogh A et al. J Mol Biol 2001 Jan 19;305(3):567-80
GO_REF:0000033 - Annotation inferences using phylogenetic trees
PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.
Marguerat S et al. Cell 2012 Oct 26;151(3):671-83
GO_REF:0000111 - Gene Ontology annotations Inferred by Curator (IC) using at least one Inferred by Sequence Similarity (ISS) annotation to support the inference
PMID:25720772 - Quantitative phosphoproteomics reveals pathways for coordination of cell growth and division by the conserved fission yeast kinase pom1.
Kettenbach AN et al. Mol Cell Proteomics 2015 May;14(5):1275-87
PMID:37792890 - Elevated levels of sphingolipid MIPC in the plasma membrane disrupt the coordination of cell growth with cell wall formation in fission yeast.
Willet AH et al. PLoS Genet 2023 Oct;19(10):e1010987
PMID:30726745 - Fission Yeast NDR/LATS Kinase Orb6 Regulates Exocytosis via Phosphorylation of the Exocyst Complex.
Tay YD et al. Cell Rep 2019 Feb 05;26(6):1654-1667.e7
PMID:24013502 - Epe1 recruits BET family bromodomain protein Bdf2 to establish heterochromatin boundaries.
Wang J et al. Genes Dev 2013 Sep 01;27(17):1886-902
PMID:21511999 - Comparative functional genomics of the fission yeasts.
Rhind N et al. Science 2011 May 20;332(6032):930-6
PMID:37970674 - SUMOylation regulates Lem2 function in centromere clustering and silencing.
Strachan J et al. J Cell Sci 2023 Dec 01;136(23)
PMID:33823663 - A TOR (target of rapamycin) and nutritional phosphoproteome of fission yeast reveals novel targets in networks conserved in humans.
Halova L et al. Open Biol 2021 Apr;11(4):200405
PMID:39367033 - Quantitative proteomics and phosphoproteomics profiling of meiotic divisions in the fission yeast Schizosaccharomyces pombe.
Sivakova B et al. Sci Rep 2024 Oct 04;14(1):23105
PMID:18257517 - Phosphoproteome analysis of fission yeast.
Wilson-Grady JT et al. J Proteome Res 2008 Mar;7(3):1088-97
GO_REF:0000024 - Manual transfer of experimentally-verified manual GO annotation data to orthologs by curator judgment of sequence similarity.
PMID:21712547 - Mitotic substrates of the kinase aurora with roles in chromatin regulation identified through quantitative phosphoproteomics of fission yeast.
Koch A et al. Sci Signal 2011 Jun 28;4(179):rs6
PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.
Hayles J et al. Open Biol 2013 May 22;3(5):130053
GO_REF:0000002 - Comments
PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.
Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623
PMID:39476757 - Characterization of Ksg1 protein kinase-dependent phosphoproteome in the fission yeast S. pombe.
Cipak L et al. Biochem Biophys Res Commun 2024 Oct 25;736:150895
PMID:29996109 - Quantitative Phosphoproteomics Reveals the Signaling Dynamics of Cell-Cycle Kinases in the Fission Yeast Schizosaccharomyces pombe.
Swaffer MP et al. Cell Rep 2018 Jul 10;24(2):503-514