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protein coding gene - put4 (SPAC869.10c) - plasma membrane proline transmembrane transporter Put4

Gene summary

Standard name
put4
Systematic ID
SPAC869.10c
Product
plasma membrane proline transmembrane transporter Put4
Organism
Schizosaccharomyces pombe (fission yeast)
UniProt ID
Q9URZ3
ORFeome ID
34/34G09
Characterisation status
biological role inferred
Feature type
mRNA gene
Genomic location
chromosome I: 5493164..5495156 forward strand

Annotation

Disease association

MONDO:0009067 - cystinuria

References:

MONDO:0009109 - lysinuric protein intolerance

References:

MONDO:0005066 - metabolic disease

References:

GO biological process

GO:1905647 - proline import across plasma membrane

References:

GO cellular component

GO:0005783 - endoplasmic reticulum

References:

GO:0005794 - Golgi apparatus

References:

GO:0005886 - plasma membrane

References:

GO molecular function

GO:0015193 - L-proline transmembrane transporter activity

References:

Modification

MOD:00046 - O-phospho-L-serine

References:

MOD:01148 - ubiquitinylated lysine

References:

Protein sequence feature

SO:0001812 - transmembrane_helix

References:

Qualitative gene expression

PomGeneEx:0000019 - protein level decreased

References:

PomGeneEx:0000018 - protein level increased

References:

PomGeneEx:0000012 - RNA level decreased

References:

PomGeneEx:0000011 - RNA level increased

References:

Quantitative gene expression

PBO:0006310 - protein level

References:

PBO:0011963 - RNA level

References:

Single locus phenotype

FYPO:0009091 - decreased cell population growth on lysine and proline nitrogen source

References:

Genotypes:

FYPO:0000250 - decreased cell population growth on proline nitrogen source

References:

Genotypes:

FYPO:0000636 - increased cell population growth rate

References:

Genotypes:

FYPO:0000764 - resistance to cycloheximide

References:

Genotypes:

FYPO:0009038 - resistance to egtazic acid

References:

Genotypes:

FYPO:0009087 - resistance to magnesium chloride and sodium dodecyl sulfate

References:

Genotypes:

FYPO:0002060 - viable vegetative cell population

References:

Genotypes:

FYPO:0002177 - viable vegetative cell with normal cell morphology

References:

Genotypes:

Taxonomic conservation

PBO:0011065 - conserved in eukaryotes

PBO:0011071 - conserved in eukaryotes only

PBO:0011064 - conserved in fungi

PBO:0011069 - conserved in metazoa

PBO:0011070 - conserved in vertebrates

Protein features

IDNameInterPro nameDB name
PF00324AA_permeaseAA-permease/SLC12A_domPFAM
PS00218AMINO_ACID_PERMEASE_1Amino_acid_permease_CSPROSITE_PATTERNS
G3DSA:1.20.1740.10:FF:000006FUNFAM
G3DSA:1.20.1740.10Amino acid/polyamine transporter IGENE3D
PTHR43341AMINO ACID PERMEASEAPC_YATPANTHER
PIRSF006060AA_transporterPIRSF

Orthologs

References / Literature

PMID:16115814 - Pas1, a G1 cyclin, regulates amino acid uptake and rescues a delay in G1 arrest in Tsc1 and Tsc2 mutants in Schizosaccharomyces pombe.
van Slegtenhorst M et al. Hum Mol Genet 2005 Oct 01;14(19):2851-8
PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.
Hayles J et al. Open Biol 2013 May 22;3(5):130053
PMID:27984744 - Survival in Quiescence Requires the Euchromatic Deployment of Clr4/SUV39H by Argonaute-Associated Small RNAs.
Joh RI et al. Mol Cell 2016 Dec 15;64(6):1088-1101
PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.
Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623
PMID:25375137 - Systematic analysis of the role of RNA-binding proteins in the regulation of RNA stability.
Hasan A et al. PLoS Genet 2014 Nov;10(11):e1004684
PMID:31626996 - Multiplexed proteome profiling of carbon source perturbations in two yeast species with SL-SP3-TMT.
Paulo JA et al. J Proteomics 2020 Jan 06;210:103531
PMID:15466417 - Regulation of leucine uptake by tor1+ in Schizosaccharomyces pombe is sensitive to rapamycin.
Weisman R et al. Genetics 2005 Feb;169(2):539-50
PMID:37787768 - Broad functional profiling of fission yeast proteins using phenomics and machine learning.
Rodríguez-López M et al. Elife 2023 Oct 03;12
PMID:16823372 - ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe.
Matsuyama A et al. Nat Biotechnol 2006 Jul;24(7):841-7
PMID:37970674 - SUMOylation regulates Lem2 function in centromere clustering and silencing.
Strachan J et al. J Cell Sci 2023 Dec 01;136(23)
PMID:38780300 - Nitrogen availability is important for preventing catastrophic mitosis in fission yeast.
Zemlianski V et al. J Cell Sci 2024 May 23;
PMID:36478272 - Translation-complex profiling of fission yeast cells reveals dynamic rearrangements of scanning ribosomal subunits upon nutritional stress.
Duncan CDS et al. Nucleic Acids Res 2022 Dec 09;50(22):13011-13025
PMID:39705284 - Proteomic and phosphoproteomic analyses reveal that TORC1 is reactivated by pheromone signaling during sexual reproduction in fission yeast.
Bérard M et al. PLoS Biol 2024 Dec 20;22(12):e3002963
PMID:25452419 - Parallel profiling of fission yeast deletion mutants for proliferation and for lifespan during long-term quiescence.
Sideri T et al. G3 (Bethesda) 2014 Dec 01;5(1):145-55
PMID:24344203 - Isp7 is a novel regulator of amino acid uptake in the TOR signaling pathway.
Laor D et al. Mol Cell Biol 2014 Mar;34(5):794-806
PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.
Marguerat S et al. Cell 2012 Oct 26;151(3):671-83
PMID:40015273 - A comprehensive Schizosaccharomyces pombe atlas of physical transcription factor interactions with proteins and chromatin.
Skribbe M et al. Mol Cell 2025 Feb 19;
GO_REF:0000024 - Manual transfer of experimentally-verified manual GO annotation data to orthologs by curator judgment of sequence similarity.
PMID:32896087 - Mitochondrial respiration is required to provide amino acids during fermentative proliferation of fission yeast.
Malecki M et al. EMBO Rep 2020 Nov 05;21(11):e50845
GO_REF:0000051 - S. pombe keyword mapping
PMID:39367033 - Quantitative proteomics and phosphoproteomics profiling of meiotic divisions in the fission yeast Schizosaccharomyces pombe.
Sivakova B et al. Sci Rep 2024 Oct 04;14(1):23105
PMID:11152613 - Predicting transmembrane protein topology with a hidden Markov model: application to complete genomes.
Krogh A et al. J Mol Biol 2001 Jan 19;305(3):567-80
PMID:17556368 - The Birt-Hogg-Dube and tuberous sclerosis complex homologs have opposing roles in amino acid homeostasis in Schizosaccharomyces pombe.
van Slegtenhorst M et al. J Biol Chem 2007 Aug 24;282(34):24583-90
PMID:21511999 - Comparative functional genomics of the fission yeasts.
Rhind N et al. Science 2011 May 20;332(6032):930-6
PB_REF:0000003 - Disease Association Curation
PMID:41844616 - Phosphoregulation of the novel hemi-arrestin MAPK scaffold Sms1 prevents untimely mating.
Sieber B et al. Nat Commun 2026 Mar 17;
PMID:20404084 - Transcriptional activation of the general amino acid permease gene per1 by the histone deacetylase Clr6 Is regulated by Oca2 kinase.
Kaufmann I et al. Mol Cell Biol 2010 Jul;30(13):3396-410
PMID:33109728 - High-Throughput Identification of Nuclear Envelope Protein Interactions in Schizosaccharomyces pombe Using an Arrayed Membrane Yeast-Two Hybrid Library.
Varberg JM et al. G3 (Bethesda) 2020 Dec 03;10(12):4649-4663
PMID:22681890 - Hierarchical modularity and the evolution of genetic interactomes across species.
Ryan CJ et al. Mol Cell 2012 Jun 08;46(5):691-704