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protein coding gene - ppr4 (SPAC8C9.06c) - mitochondrial PPR repeat protein, translation activator for Cox1 Ppr4

Gene summary

Standard name
ppr4
Systematic ID
SPAC8C9.06c
Product
mitochondrial PPR repeat protein, translation activator for Cox1 Ppr4
Organism
Schizosaccharomyces pombe (fission yeast)
Synonyms
srd2
UniProt ID
O14275
ORFeome ID
29/29D10
Characterisation status
biological role published
Feature type
mRNA gene
Genomic location
chromosome I: 3649467..3654006 reverse strand

Annotation

GO biological process

GO:0140053 - mitochondrial gene expression

References:

GO:0032543 - mitochondrial translation

References:

GO:0070124 - mitochondrial translational initiation

References:

GO:0070134 - positive regulation of mitochondrial translational initiation

References:

GO cellular component

GO:0005737 - cytoplasm

References:

GO:0099616 - extrinsic component of matrix side of mitochondrial inner membrane

References:

GO:0005739 - mitochondrion

References:

GO:0180049 - Mrh5C translation activator complex

References:

GO molecular function

GO:0048027 - mRNA 5'-UTR binding

References:

GO:0160134 - protein-RNA sequence-specific adaptor activity

References:

GO:0008494 - translation activator activity

References:

GO:0045182 - translation regulator activity

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Multi-locus phenotype

FYPO:0000251 - decreased cell population growth on galactose carbon source

References:

Genotypes:

FYPO:0003731 - normal growth on galactose carbon source

References:

Genotypes:

Protein features

PBO:0111828 - PPR repeats

References:

Protein sequence feature

SO:0001808 - mitochondrial_targeting_signal

References:

SO:0000418 - signal_peptide

Qualitative gene expression

PomGeneEx:0000018 - protein level increased

References:

PomGeneEx:0000027 - ribosomal density decreased

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PomGeneEx:0000011 - RNA level increased

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Quantitative gene expression

PBO:0011963 - RNA level

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Single locus phenotype

FYPO:0003730 - abolished cell population growth on galactose carbon source

References:

Genotypes:

FYPO:0001934 - abolished cell population growth on glycerol carbon source

References:

Genotypes:

FYPO:0000251 - decreased cell population growth on galactose carbon source

References:

Genotypes:

FYPO:0000684 - decreased cell population growth on glycerol carbon source

References:

Genotypes:

FYPO:0000708 - decreased mating efficiency

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Genotypes:

FYPO:0000835 - decreased protein level

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Genotypes:

FYPO:0001355 - decreased vegetative cell population growth

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Genotypes:

FYPO:0009007 - decreased vegetative cell population viability

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Genotypes:

FYPO:0009012 - decreased vegetative cell population viability on galactose carbon source

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Genotypes:

FYPO:0009014 - decreased vegetative cell population viability on xylose carbon source

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Genotypes:

FYPO:0001919 - fragmented nucleus during vegetative growth

References:

Genotypes:

FYPO:0005760 - increased cell-cell adhesion

References:

Genotypes:

FYPO:0002006 - increased cellular iron level

References:

Genotypes:

FYPO:0003004 - increased cellular reactive oxygen species level during vegetative growth

References:

Genotypes:

FYPO:0000377 - increased frequency of apoptosis

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Genotypes:

FYPO:0003335 - increased galactose-specific flocculation

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Genotypes:

FYPO:0006597 - increased level of cell wall organization gene mRNA during vegetative growth

References:

Genotypes:

FYPO:0006596 - increased level of flocculin gene mRNA during vegetative growth

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Genotypes:

FYPO:0005823 - increased phosphatidylserine externalization

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Genotypes:

FYPO:0005824 - increased plasma membrane permeability

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Genotypes:

FYPO:0003776 - increased pseudohyphal growth

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Genotypes:

FYPO:0000245 - loss of viability in stationary phase

References:

Genotypes:

FYPO:0005231 - loss of viability in stationary phase upon glucose starvation

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Genotypes:

FYPO:0004162 - loss of viability upon glucose starvation

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Genotypes:

FYPO:0000256 - mutator

References:

Genotypes:

FYPO:0004529 - normal mitochondrial translation

References:

Genotypes:

FYPO:0000833 - normal protein level during vegetative growth

References:

Genotypes:

FYPO:0000703 - normal protein-protein interaction

References:

Genotypes:

FYPO:0000840 - normal RNA level

References:

Genotypes:

FYPO:0009038 - resistance to egtazic acid

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Genotypes:

FYPO:0001103 - resistance to hydrogen peroxide

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Genotypes:

FYPO:0009085 - resistance to lithium chloride and sodium dodecyl sulfate

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Genotypes:

FYPO:0009087 - resistance to magnesium chloride and sodium dodecyl sulfate

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Genotypes:

FYPO:0009043 - resistance to potassium chloride and sodium dodecyl sulfate

References:

Genotypes:

FYPO:0003383 - resistance to tert-butyl hydroperoxide

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Genotypes:

FYPO:0001097 - sensitive to amitrole

References:

Genotypes:

FYPO:0000095 - sensitive to bleomycin

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Genotypes:

FYPO:0000098 - sensitive to calcium

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Genotypes:

FYPO:0007926 - sensitive to calcofluor and potassium chloride

References:

Genotypes:

FYPO:0000099 - sensitive to canavanine

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Genotypes:

FYPO:0000104 - sensitive to cycloheximide

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Genotypes:

FYPO:0000799 - sensitive to diamide

References:

Genotypes:

FYPO:0007931 - sensitive to egtazic acid

References:

Genotypes:

FYPO:0000842 - sensitive to ethanol during vegetative growth

References:

Genotypes:

FYPO:0007928 - sensitive to ethylenediaminetetraacetic acid

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Genotypes:

FYPO:0000785 - sensitive to formamide

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Genotypes:

FYPO:0000087 - sensitive to hydrogen peroxide

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Genotypes:

FYPO:0005825 - sensitive to iron

References:

Genotypes:

FYPO:0001719 - sensitive to lithium

References:

Genotypes:

FYPO:0000089 - sensitive to methyl methanesulfonate

References:

Genotypes:

FYPO:0007929 - sensitive to phloxine B

References:

Genotypes:

FYPO:0007930 - sensitive to phloxine B and hydrogen peroxide

References:

Genotypes:

FYPO:0001214 - sensitive to potassium chloride

References:

Genotypes:

FYPO:0000112 - sensitive to sorbitol

References:

Genotypes:

FYPO:0000797 - sensitive to tert-butyl hydroperoxide

References:

Genotypes:

FYPO:0001457 - sensitive to tunicamycin

References:

Genotypes:

FYPO:0000115 - sensitive to valproic acid

References:

Genotypes:

FYPO:0003656 - sensitive to vanadate

References:

Genotypes:

FYPO:0003810 - small fragmented mitochondria present in increased numbers

References:

Genotypes:

FYPO:0002060 - viable vegetative cell population

References:

Genotypes:

FYPO:0002177 - viable vegetative cell with normal cell morphology

References:

Genotypes:

Taxonomic conservation

PBO:0011065 - conserved in eukaryotes

PBO:0011071 - conserved in eukaryotes only

PBO:0011064 - conserved in fungi

Protein features

IDNameInterPro nameDB name
PF01535PPRPPR_rptPfam
G3DSA:1.25.40.10TPR-like_helical_dom_sfCATH-Gene3D
PTHR47938PANTHER

Orthologs

References / Literature

PMID:29878109 - Disruption of ppr3, ppr4, ppr6 or ppr10 induces flocculation and filamentous growth in Schizosaccharomyces pombe.
Su Y et al. FEMS Microbiol Lett 2018 Aug 01;365(16)
PMID:21727087 - A genome wide study in fission yeast reveals nine PPR proteins that regulate mitochondrial gene expression.
Kühl I et al. Nucleic Acids Res 2011 Oct;39(18):8029-41
PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.
Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623
PMID:27886462 - Loss of ppr3, ppr4, ppr6, or ppr10 perturbs iron homeostasis and leads to apoptotic cell death in Schizosaccharomyces pombe.
Su Y et al. FEBS J 2017 Jan;284(2):324-337
PMID:34634819 - Translational activators and mitoribosomal isoforms cooperate to mediate mRNA-specific translation in Schizosaccharomyces pombe mitochondria.
Herbert CJ et al. Nucleic Acids Res 2021 Nov 08;49(19):11145-11166
PMID:23950735 - Global analysis of fission yeast mating genes reveals new autophagy factors.
Sun LL et al. PLoS Genet 2013;9(8):e1003715
PMID:27918601 - Identifying genes required for respiratory growth of fission yeast.
Malecki M et al. Wellcome Open Res 2016;1:12
PMID:39367033 - Quantitative proteomics and phosphoproteomics profiling of meiotic divisions in the fission yeast Schizosaccharomyces pombe.
Sivakova B et al. Sci Rep 2024 Oct 04;14(1):23105
PMID:21511999 - Comparative functional genomics of the fission yeasts.
Rhind N et al. Science 2011 May 20;332(6032):930-6
PMID:18665268 - Mitochondrial dysfunction increases oxidative stress and decreases chronological life span in fission yeast.
Zuin A et al. PLoS One 2008 Jul 30;3(7):e2842
GO_REF:0000024 - Manual transfer of experimentally-verified manual GO annotation data to orthologs by curator judgment of sequence similarity.
PMID:33313903 - Ribosome profiling reveals ribosome stalling on tryptophan codons and ribosome queuing upon oxidative stress in fission yeast.
Rubio A et al. Nucleic Acids Res 2021 Jan 11;49(1):383-399
PMID:34984977 - Functional profiling of long intergenic non-coding RNAs in fission yeast.
Rodriguez-Lopez M et al. Elife 2022 Jan 05;11
PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.
Hayles J et al. Open Biol 2013 May 22;3(5):130053
PMID:36408920 - UniProt: the Universal Protein Knowledgebase in 2023.
UniProt Consortium Nucleic Acids Res 2023 Jan 06;51(D1):D523-D531
PMID:37787768 - Broad functional profiling of fission yeast proteins using phenomics and machine learning.
Rodríguez-López M et al. Elife 2023 Oct 03;12
PMID:30824696 - Systematic analysis reveals the prevalence and principles of bypassable gene essentiality.
Li J et al. Nat Commun 2019 Mar 01;10(1):1002
PMID:38499152 - Sls1 and Mtf2 mediate the assembly of the Mrh5C complex required for activation of cox1 mRNA translation.
Wang Y et al. J Biol Chem 2024 Mar 16;:107176
PMID:22349564 - Schizosaccharomyces pombe homologs of the Saccharomyces cerevisiae mitochondrial proteins Cbp6 and Mss51 function at a post-translational step of respiratory complex biogenesis.
Kühl I et al. Mitochondrion 2012 May;12(3):381-90
PMID:27664110 - Loss of Msp1p in Schizosaccharomyces pombe induces a ROS-dependent nuclear mutator phenotype that affects mitochondrial fission genes.
Delerue T et al. FEBS Lett 2016 Oct;590(20):3544-3558
PMID:16823372 - ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe.
Matsuyama A et al. Nat Biotechnol 2006 Jul;24(7):841-7
PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.
Marguerat S et al. Cell 2012 Oct 26;151(3):671-83