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protein coding gene - msh3 (SPAC8F11.03) - MutS protein homolog 3

Gene summary

Standard name
msh3
Systematic ID
SPAC8F11.03
Product
MutS protein homolog 3
Organism
Schizosaccharomyces pombe (fission yeast)
Synonyms
swi4
UniProt ID
P26359
ORFeome ID
47/47G08
Characterisation status
biological role published
Feature type
mRNA gene
Genomic location
chromosome I: 2855753..2859170 forward strand

Annotation

Disease association

MONDO:0011962 - endometrial cancer

References:

MONDO:0044300 - familial adenomatous polyposis 4

References:

GO biological process

GO:0007534 - gene conversion at mating-type locus

References:

GO:0043570 - maintenance of DNA repeat elements

References:

GO:0006298 - mismatch repair

References:

GO:0007131 - reciprocal meiotic recombination

References:

GO:0006298 - mismatch repair

References:

GO cellular component

GO:0000228 - nuclear chromosome

References:

GO:0005634 - nucleus

References:

GO:0035861 - site of double-strand break

References:

GO molecular function

GO:0005524 - ATP binding

References:

GO:0016887 - ATP hydrolysis activity

References:

GO:0140664 - ATP-dependent DNA damage sensor activity

References:

GO:0000406 - double-strand/single-strand DNA junction binding

References:

GO:0000404 - heteroduplex DNA loop binding

References:

GO:0000403 - Y-form DNA binding

References:

Modification

MOD:00046 - O-phospho-L-serine

References:

Multi-locus phenotype

FYPO:0001740 - increased gross chromosomal rearrangement

References:

Genotypes:

Qualitative gene expression

PomGeneEx:0000019 - protein level decreased

References:

Quantitative gene expression

PBO:0011963 - RNA level

References:

Single locus phenotype

FYPO:0003659 - abnormal mating type switching resulting in duplication or deletion in mating-type region

References:

Genotypes:

FYPO:0000469 - abolished mating type switching

References:

Genotypes:

FYPO:0003352 - decreased DNA double-strand break formation at mating-type locus

References:

Genotypes:

FYPO:0006810 - decreased gross chromosomal rearrangement

References:

Genotypes:

FYPO:0000708 - decreased mating efficiency

References:

Genotypes:

FYPO:0000470 - decreased mating type switching

References:

Genotypes:

FYPO:0000584 - decreased sporulation frequency

References:

Genotypes:

FYPO:0009007 - decreased vegetative cell population viability

References:

Genotypes:

FYPO:0001821 - increased number of heterothallic h+ cells

References:

Genotypes:

FYPO:0004344 - increased viability upon nitrogen starvation

References:

Genotypes:

FYPO:0001822 - mating cassette duplication

References:

Genotypes:

FYPO:0004295 - multiseptate cell

References:

Genotypes:

FYPO:0003353 - normal DNA double-strand break formation at mating-type locus

References:

Genotypes:

FYPO:0004993 - normal spore germination frequency

References:

Genotypes:

FYPO:0009079 - resistance to calcofluor and sodium dodecyl sulfate

References:

Genotypes:

FYPO:0009038 - resistance to egtazic acid

References:

Genotypes:

FYPO:0003383 - resistance to tert-butyl hydroperoxide

References:

Genotypes:

FYPO:0006680 - sensitive to bisphenol A

References:

Genotypes:

FYPO:0000096 - sensitive to cadmium

References:

Genotypes:

FYPO:0000799 - sensitive to diamide

References:

Genotypes:

FYPO:0000089 - sensitive to methyl methanesulfonate

References:

Genotypes:

FYPO:0003656 - sensitive to vanadate

References:

Genotypes:

FYPO:0003612 - viable spore population

References:

Genotypes:

FYPO:0002060 - viable vegetative cell population

References:

Genotypes:

FYPO:0002177 - viable vegetative cell with normal cell morphology

References:

Genotypes:

Taxonomic conservation

PBO:0011072 - conserved in archaea

PBO:0011067 - conserved in bacteria

PBO:0011065 - conserved in eukaryotes

PBO:0011064 - conserved in fungi

PBO:0011069 - conserved in metazoa

PBO:0011070 - conserved in vertebrates

Protein features

IDNameInterPro nameDB name
PF00488MutS_VDNA_mismatch_repair_MutS_CPfam
PF01624MutS_IDNA_mismatch_repair_MutS-lik_NPfam
PF05188MutS_IIDNA_mmatch_repair_MutS_con_domPfam
PF05190MutS_IVDNA_mismatch_repair_MutS_clampPfam
PF05192MutS_IIIDNA_mismatch_repair_MutS_corePfam
cd03287ABC_MSH3_eukCDD
PS00486DNA_MISMATCH_REPAIR_2DNA_mismatch_repair_MutS_CPROSITE patterns
SM00533MUTSdDNA_mismatch_repair_MutS_coreSMART
SM00534MUTSacDNA_mismatch_repair_MutS_CSMART
G3DSA:1.10.1420.10:FF:000004CATH-FunFam
G3DSA:3.30.420.110:FF:000010CATH-FunFam
G3DSA:3.40.1170.10:FF:000004CATH-FunFam
G3DSA:3.40.50.300:FF:000870CATH-FunFam
G3DSA:1.10.1420.10CATH-Gene3D
G3DSA:3.30.420.110MutS_con_dom_sfCATH-Gene3D
G3DSA:3.40.1170.10DNA_mismatch_repair_MutS_NCATH-Gene3D
G3DSA:3.40.50.300P-loop_NTPaseCATH-Gene3D
SSF48334DNA_mismatch_repair_MutS_sfSUPERFAMILY
SSF52540P-loop_NTPaseSUPERFAMILY
SSF55271DNA_mismatch_repair_MutS_NSUPERFAMILY
PTHR11361MutSPANTHER
PIRSF037677DNA_mis_repair_Msh6DNA_mismatch_repair_MutS/MSHPIRSF
NF003810mutSNCBIFAM
CoilCOILS

Orthologs

References / Literature

GO_REF:0000033 - Annotation inferences using phylogenetic trees
PMID:22681890 - Hierarchical modularity and the evolution of genetic interactomes across species.
Ryan CJ et al. Mol Cell 2012 Jun 08;46(5):691-704
PMID:23779158 - The proteasome factor Bag101 binds to Rad22 and suppresses homologous recombination.
Saito Y et al. Sci Rep 2013;3:2022
PMID:25720772 - Quantitative phosphoproteomics reveals pathways for coordination of cell growth and division by the conserved fission yeast kinase pom1.
Kettenbach AN et al. Mol Cell Proteomics 2015 May;14(5):1275-87
PMID:32355220 - DNA replication machinery prevents Rad52-dependent single-strand annealing that leads to gross chromosomal rearrangements at centromeres.
Onaka AT et al. Commun Biol 2020 Apr 30;3(1):202
GO_REF:0000024 - Manual transfer of experimentally-verified manual GO annotation data to orthologs by curator judgment of sequence similarity.
PMID:30647105 - Comparative Genomic Screen in Two Yeasts Reveals Conserved Pathways in the Response Network to Phenol Stress.
Alhoch B et al. G3 (Bethesda) 2019 Mar 07;9(3):639-650
PMID:1317550 - The swi4+ gene of Schizosaccharomyces pombe encodes a homologue of mismatch repair enzymes.
Fleck O et al. Nucleic Acids Res 1992 May 11;20(9):2271-8
PMID:15548596 - Analysis of mutant phenotypes and splicing defects demonstrates functional collaboration between the large and small subunits of the essential splicing factor U2AF in vivo.
Webb CJ et al. Mol Biol Cell 2005 Feb;16(2):584-96
PMID:25452419 - Parallel profiling of fission yeast deletion mutants for proliferation and for lifespan during long-term quiescence.
Sideri T et al. G3 (Bethesda) 2014 Dec 01;5(1):145-55
PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.
Hayles J et al. Open Biol 2013 May 22;3(5):130053
PMID:34984977 - Functional profiling of long intergenic non-coding RNAs in fission yeast.
Rodriguez-Lopez M et al. Elife 2022 Jan 05;11
PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.
Marguerat S et al. Cell 2012 Oct 26;151(3):671-83
PMID:37787768 - Broad functional profiling of fission yeast proteins using phenomics and machine learning.
Rodríguez-López M et al. Elife 2023 Oct 03;12
PMID:23628481 - A proteome-wide visual screen identifies fission yeast proteins localizing to DNA double-strand breaks.
Yu Y et al. DNA Repair (Amst) 2013 Jun 01;12(6):433-43
PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.
Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623
PMID:21511999 - Comparative functional genomics of the fission yeasts.
Rhind N et al. Science 2011 May 20;332(6032):930-6
GO_REF:0000111 - Gene Ontology annotations Inferred by Curator (IC) using at least one Inferred by Sequence Similarity (ISS) annotation to support the inference
PMID:18931302 - Significant conservation of synthetic lethal genetic interaction networks between distantly related eukaryotes.
Dixon SJ et al. Proc Natl Acad Sci U S A 2008 Oct 28;105(43):16653-8
PMID:24719968 - Switching genes in Schizosaccharomyces pombe.
Gutz H et al. Curr Genet 1985;9(5):325-31
PMID:29852001 - New insights into donor directionality of mating-type switching in Schizosaccharomyces pombe.
Maki T et al. PLoS Genet 2018 May;14(5):e1007424
PMID:28228545 - Ablation of RNA interference and retrotransposons accompany acquisition and evolution of transposases to heterochromatin protein CENPB.
Upadhyay U et al. Mol Biol Cell 2017 Apr 15;28(8):1132-1146
GO_REF:0000002 - Comments
PMID:31712578 - DNA sequence differences are determinants of meiotic recombination outcome.
Brown SD et al. Sci Rep 2019 Nov 11;9(1):16446
PMID:39367033 - Quantitative proteomics and phosphoproteomics profiling of meiotic divisions in the fission yeast Schizosaccharomyces pombe.
Sivakova B et al. Sci Rep 2024 Oct 04;14(1):23105
PMID:16823372 - ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe.
Matsuyama A et al. Nat Biotechnol 2006 Jul;24(7):841-7
PMID:28341698 - Schizosaccharomyces pombe MutSα and MutLα Maintain Stability of Tetra-Nucleotide Repeats and Msh3 of Hepta-Nucleotide Repeats.
Villahermosa D et al. G3 (Bethesda) 2017 May 05;7(5):1463-1473
PMID:34292936 - Fission yeast Rad8/HLTF facilitates Rad52-dependent chromosomal rearrangements through PCNA lysine 107 ubiquitination.
Su J et al. PLoS Genet 2021 Jul;17(7):e1009671
PMID:11333219 - Control of GT repeat stability in Schizosaccharomyces pombe by mismatch repair factors.
Mansour AA et al. Genetics 2001 May;158(1):77-85
PB_REF:0000006 - Disease associations from Monarch via human-pombe orthologs
PMID:11333218 - Requirement for Msh6, but not for Swi4 (Msh3), in Msh2-dependent repair of base-base mismatches and mononucleotide loops in Schizosaccharomyces pombe.
Tornier C et al. Genetics 2001 May;158(1):65-75
PMID:6587363 - Genes required for initiation and resolution steps of mating-type switching in fission yeast.
Egel R et al. Proc Natl Acad Sci U S A 1984 Jun;81(11):3481-5
PMID:27611590 - Pfh1 Is an Accessory Replicative Helicase that Interacts with the Replisome to Facilitate Fork Progression and Preserve Genome Integrity.
McDonald KR et al. PLoS Genet 2016 Sep;12(9):e1006238
PMID:2076550 - A mutated swi4 gene causes duplications in the mating-type region of Schizosaccharomyces pombe.
Fleck O et al. Curr Genet 1990 Dec;18(6):501-9
PMID:28410370 - A systematic screen for morphological abnormalities during fission yeast sexual reproduction identifies a mechanism of actin aster formation for cell fusion.
Dudin O et al. PLoS Genet 2017 Apr;13(4):e1006721
PMID:10716938 - Fission yeast switches mating type by a replication-recombination coupled process.
Arcangioli B et al. EMBO J 2000 Mar 15;19(6):1389-96