PomBase home

protein coding gene - fml1 (SPAC9.05) - ATP-dependent 3' to 5' DNA helicase, FANCM ortholog Fml1

Gene summary

Standard name
fml1
Systematic ID
SPAC9.05
Product
ATP-dependent 3' to 5' DNA helicase, FANCM ortholog Fml1
Organism
Schizosaccharomyces pombe (fission yeast)
Synonyms
mfh1
UniProt ID
Q9UT23
ORFeome ID
38/38F02
Characterisation status
biological role published
Feature type
mRNA gene
Genomic location
chromosome I: 1470834..1476275 forward strand

Annotation

Disease association

MONDO:0054862 - premature ovarian failure 15

References:

MONDO:0054732 - spermatogenic failure 28

References:

GO biological process

GO:0000732 - DNA strand displacement

References:

GO:0045003 - double-strand break repair via synthesis-dependent strand annealing

References:

GO:1902346 - meiotic strand displacement involved in double-strand break repair via SDSA

References:

GO:1903461 - Okazaki fragment processing involved in mitotic DNA replication

References:

GO:0007131 - reciprocal meiotic recombination

References:

GO:0036298 - recombinational interstrand cross-link repair

References:

GO:0031297 - replication fork processing

References:

GO cellular component

GO:0005829 - cytosol

References:

GO:0071821 - FANCM-MHF complex

References:

GO:0005730 - nucleolus

References:

GO:0005634 - nucleus

References:

GO:0035861 - site of double-strand break

References:

GO molecular function

GO:0043138 - 3'-5' DNA helicase activity

References:

GO:0005524 - ATP binding

References:

GO:0003690 - double-stranded DNA binding

References:

GO:0000400 - four-way junction DNA binding

References:

GO:0009378 - four-way junction helicase activity

References:

Modification

MOD:00046 - O-phospho-L-serine

References:

MOD:00047 - O-phospho-L-threonine

References:

Multi-locus phenotype

FYPO:0005658 - abnormal frequency of meiotic crossover associated with gene conversion

References:

Genotypes:

FYPO:0004159 - abnormal homologous chromosome segregation

References:

Genotypes:

FYPO:0005660 - decreased frequency of meiotic crossover associated with gene conversion

References:

Genotypes:

FYPO:0002485 - decreased intergenic meiotic recombination

References:

Genotypes:

FYPO:0003179 - decreased intragenic meiotic recombination

References:

Genotypes:

FYPO:0000581 - decreased spore germination frequency

References:

Genotypes:

FYPO:0001355 - decreased vegetative cell population growth

References:

Genotypes:

FYPO:0006419 - increased duration of Rad51 focus presence during meiotic cell cycle

References:

Genotypes:

FYPO:0005659 - increased frequency of meiotic crossover associated with gene conversion

References:

Genotypes:

FYPO:0006280 - increased mitotic recombination with crossover formation at centromere

References:

Genotypes:

FYPO:0004993 - normal spore germination frequency

References:

Genotypes:

FYPO:0005775 - sensitive to acetaldehyde

References:

Genotypes:

FYPO:0000102 - sensitive to cisplatin

References:

Genotypes:

FYPO:0007330 - sensitive to formaldehyde

References:

Genotypes:

Protein features

PBO:0111747 - DEAD/DEAH box helicase

Quantitative gene expression

PBO:0006310 - protein level

References:

PBO:0011963 - RNA level

References:

Single locus phenotype

FYPO:0009099 - decreased cell population growth on mannitol carbon source

References:

Genotypes:

FYPO:0006920 - decreased DNA recombination at mitotic DNA replication fork barriers

References:

Genotypes:

FYPO:0002485 - decreased intergenic meiotic recombination

References:

Genotypes:

FYPO:0002486 - decreased meiotic strand displacement

References:

Genotypes:

FYPO:0000581 - decreased spore germination frequency

References:

Genotypes:

FYPO:0000584 - decreased sporulation frequency

References:

Genotypes:

FYPO:0004806 - incomplete cell wall disassembly at cell fusion site

References:

Genotypes:

FYPO:0000167 - increased DNA recombination at mitotic DNA replication fork barriers

References:

Genotypes:

FYPO:0005659 - increased frequency of meiotic crossover associated with gene conversion

References:

Genotypes:

FYPO:0001740 - increased gross chromosomal rearrangement

References:

Genotypes:

FYPO:0002484 - increased intergenic meiotic recombination

References:

Genotypes:

FYPO:0006280 - increased mitotic recombination with crossover formation at centromere

References:

Genotypes:

FYPO:0005777 - increased number of Rad52 foci during cellular response to acetaldehyde

References:

Genotypes:

FYPO:0000972 - increased number of Rad52 foci during vegetative growth

References:

Genotypes:

FYPO:0004295 - multiseptate cell

References:

Genotypes:

FYPO:0007007 - normal DNA recombination frequency downstream of mitotic DNA replication fork barriers

References:

Genotypes:

FYPO:0007331 - normal growth on formaldehyde

References:

Genotypes:

FYPO:0001164 - normal growth on glucose carbon source

References:

Genotypes:

FYPO:0000957 - normal growth on methyl methanesulfonate

References:

Genotypes:

FYPO:0005578 - normal intergenic meiotic recombination

References:

Genotypes:

FYPO:0003891 - normal intragenic meiotic recombination

References:

Genotypes:

FYPO:0004993 - normal spore germination frequency

References:

Genotypes:

FYPO:0000763 - resistance to cadmium

References:

Genotypes:

FYPO:0000764 - resistance to cycloheximide

References:

Genotypes:

FYPO:0009038 - resistance to egtazic acid

References:

Genotypes:

FYPO:0009043 - resistance to potassium chloride and sodium dodecyl sulfate

References:

Genotypes:

FYPO:0006680 - sensitive to bisphenol A

References:

Genotypes:

FYPO:0000095 - sensitive to bleomycin

References:

Genotypes:

FYPO:0000102 - sensitive to cisplatin

References:

Genotypes:

FYPO:0007330 - sensitive to formaldehyde

References:

Genotypes:

FYPO:0000088 - sensitive to hydroxyurea

References:

Genotypes:

FYPO:0009084 - sensitive to lithium chloride and methyl methanesulfonate

References:

Genotypes:

FYPO:0000089 - sensitive to methyl methanesulfonate

References:

Genotypes:

FYPO:0009082 - sensitive to potassium chloride and methyl methanesulfonate

References:

Genotypes:

FYPO:0000925 - unequal meiotic chromosome segregation

References:

Genotypes:

FYPO:0003612 - viable spore population

References:

Genotypes:

FYPO:0002060 - viable vegetative cell population

References:

Genotypes:

FYPO:0002177 - viable vegetative cell with normal cell morphology

References:

Genotypes:

Taxonomic conservation

PBO:0011067 - conserved in bacteria

PBO:0011065 - conserved in eukaryotes

PBO:0011064 - conserved in fungi

PBO:0011069 - conserved in metazoa

PBO:0011070 - conserved in vertebrates

PBO:0006222 - predominantly single copy (one to one)

Protein features

IDNameInterPro nameDB name
PF00271Helicase_CHelicase_C-likePFAM
PF00270DEADDEAD/DEAH_box_helicase_domPFAM
cd18033DEXDc_FANCMFANCM_DEXDcCDD
cd12091FANCM_IDFANCM/Mph1-like_IDCDD
cd18801SF2_C_FANCM_HefCDD
PS00690DEAH_ATP_HELICASEDNA/RNA_helicase_DEAH_CSPROSITE_PATTERNS
PS51194HELICASE_CTERHelicase_C-likePROSITE_PROFILES
PS51192HELICASE_ATP_BIND_1Helicase_ATP-bdPROSITE_PROFILES
SM00490helicmild6Helicase_C-likeSMART
SM00487ultradead3Helicase_ATP-bdSMART
G3DSA:3.40.50.300:FF:000861FUNFAM
G3DSA:1.20.1320.20:FF:000001FUNFAM
G3DSA:3.40.50.300:FF:002583FUNFAM
SSF52540P-loop containing nucleoside triphosphate hydrolasesP-loop_NTPaseSUPERFAMILY
G3DSA:3.40.50.300P-loop_NTPaseGENE3D
G3DSA:1.20.1320.20hef helicase domainGENE3D
PTHR14025FANCONI ANEMIA GROUP M FANCM FAMILY MEMBERPANTHER
mobidb-lite-Disorderdisorder_predictionMOBIDB-Disorder
mobidb-lite-Polyampholytedisorder_predictionMOBIDB-Polyampholyte

Orthologs

References / Literature

PMID:23628481 - A proteome-wide visual screen identifies fission yeast proteins localizing to DNA double-strand breaks.
Yu Y et al. DNA Repair (Amst) 2013 Jun 01;12(6):433-43
PMID:30824696 - Systematic analysis reveals the prevalence and principles of bypassable gene essentiality.
Li J et al. Nat Commun 2019 Mar 01;10(1):1002
PMID:20347428 - A histone-fold complex and FANCM form a conserved DNA-remodeling complex to maintain genome stability.
Yan Z et al. Mol Cell 2010 Mar 26;37(6):865-78
PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.
Hayles J et al. Open Biol 2013 May 22;3(5):130053
GO_REF:0000024 - Manual transfer of experimentally-verified manual GO annotation data to orthologs by curator judgment of sequence similarity.
PMID:22681890 - Hierarchical modularity and the evolution of genetic interactomes across species.
Ryan CJ et al. Mol Cell 2012 Jun 08;46(5):691-704
GO_REF:0000002 - Comments
PMID:24463365 - Systematic screen for mutants resistant to TORC1 inhibition in fission yeast reveals genes involved in cellular ageing and growth.
Rallis C et al. Biol Open 2014 Feb 15;3(2):161-71
PMID:30726745 - Fission Yeast NDR/LATS Kinase Orb6 Regulates Exocytosis via Phosphorylation of the Exocyst Complex.
Tay YD et al. Cell Rep 2019 Feb 05;26(6):1654-1667.e7
PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.
Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623
PMID:31563844 - The roles of fission yeast exonuclease 5 in nuclear and mitochondrial genome stability.
Sparks JL et al. DNA Repair (Amst) 2019 Nov;83:102720
PMID:22723423 - The fission yeast FANCM ortholog directs non-crossover recombination during meiosis.
Lorenz A et al. Science 2012 Jun 22;336(6088):1585-8
PMID:32269268 - Abo1 is required for the H3K9me2 to H3K9me3 transition in heterochromatin.
Dong W et al. Sci Rep 2020 Apr 08;10(1):6055
PMID:30667359 - Factors affecting template switch recombination associated with restarted DNA replication.
Jalan M et al. Elife 2019 Jan 22;8
PMID:18818364 - Conservation and rewiring of functional modules revealed by an epistasis map in fission yeast.
Roguev A et al. Science 2008 Oct 17;322(5900):405-10
PMID:31712578 - DNA sequence differences are determinants of meiotic recombination outcome.
Brown SD et al. Sci Rep 2019 Nov 11;9(1):16446
PMID:24192486 - The conserved Fanconi anemia nuclease Fan1 and the SUMO E3 ligase Pli1 act in two novel Pso2-independent pathways of DNA interstrand crosslink repair in yeast.
Fontebasso Y et al. DNA Repair (Amst) 2013 Dec;12(12):1011-23
PMID:32034465 - Genetic investigation of formaldehyde-induced DNA damage response in Schizosaccharomyces pombe.
Anandarajan V et al. Curr Genet 2020 Jun;66(3):593-605
PMID:19264558 - Screening a genome-wide S. pombe deletion library identifies novel genes and pathways involved in genome stability maintenance.
Deshpande GP et al. DNA Repair (Amst) 2009 May 01;8(5):672-9
PMID:37787768 - Broad functional profiling of fission yeast proteins using phenomics and machine learning.
Rodríguez-López M et al. Elife 2023 Oct 03;12
PMID:28977643 - Regulation of mitotic recombination between DNA repeats in centromeres.
Zafar F et al. Nucleic Acids Res 2017 Nov 02;45(19):11222-11235
GO_REF:0000033 - Annotation inferences using phylogenetic trees
PMID:30647105 - Comparative Genomic Screen in Two Yeasts Reveals Conserved Pathways in the Response Network to Phenol Stress.
Alhoch B et al. G3 (Bethesda) 2019 Mar 07;9(3):639-650
PMID:28410370 - A systematic screen for morphological abnormalities during fission yeast sexual reproduction identifies a mechanism of actin aster formation for cell fusion.
Dudin O et al. PLoS Genet 2017 Apr;13(4):e1006721
PMID:27611590 - Pfh1 Is an Accessory Replicative Helicase that Interacts with the Replisome to Facilitate Fork Progression and Preserve Genome Integrity.
McDonald KR et al. PLoS Genet 2016 Sep;12(9):e1006238
PMID:28586299 - Inter-Fork Strand Annealing causes genomic deletions during the termination of DNA replication.
Morrow CA et al. Elife 2017 Jun 06;6
PMID:25414342 - Rad51/Dmc1 paralogs and mediators oppose DNA helicases to limit hybrid DNA formation and promote crossovers during meiotic recombination.
Lorenz A et al. Nucleic Acids Res 2014 Dec 16;42(22):13723-35
PMID:27687866 - Genetic controls of DNA damage avoidance in response to acetaldehyde in fission yeast.
Noguchi C et al. Cell Cycle 2017 Jan 02;16(1):45-58
PMID:22540037 - Predicting the fission yeast protein interaction network.
Pancaldi V et al. G3 (Bethesda) 2012 Apr;2(4):453-67
PMID:18851838 - The FANCM ortholog Fml1 promotes recombination at stalled replication forks and limits crossing over during DNA double-strand break repair.
Sun W et al. Mol Cell 2008 Oct 10;32(1):118-28
PMID:21511999 - Comparative functional genomics of the fission yeasts.
Rhind N et al. Science 2011 May 20;332(6032):930-6
PMID:24875629 - Essential domains of Schizosaccharomyces pombe Rad8 required for DNA damage response.
Ding L et al. G3 (Bethesda) 2014 May 28;4(8):1373-84
PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.
Marguerat S et al. Cell 2012 Oct 26;151(3):671-83
PMID:16823372 - ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe.
Matsuyama A et al. Nat Biotechnol 2006 Jul;24(7):841-7
PMID:25720772 - Quantitative phosphoproteomics reveals pathways for coordination of cell growth and division by the conserved fission yeast kinase pom1.
Kettenbach AN et al. Mol Cell Proteomics 2015 May;14(5):1275-87
PB_REF:0000006 - Disease associations from Monarch via human-pombe orthologs
PMID:27304859 - Dbl2 Regulates Rad51 and DNA Joint Molecule Metabolism to Ensure Proper Meiotic Chromosome Segregation.
Polakova S et al. PLoS Genet 2016 Jun;12(6):e1006102