protein coding gene - rlc1 (SPAC926.03) - myosin II regulatory light chain Rlc1

Gene summary

Standard name

rlc1

Systematic ID

SPAC926.03

Product

myosin II regulatory light chain Rlc1

Organism

Schizosaccharomyces pombe (fission yeast)

UniProt ID

Q9UUG5

ORFeome ID

05/05F10

Characterisation status

biological role published

Feature type

mRNA gene

Genomic location

chromosome I: 3887262..3888497 forward strand

Annotation

Protein features

Orthologs

• MYL9 (HGNC:15754) Homo sapiens
• MYL12A (HGNC:16701) Homo sapiens
• MYL7 (HGNC:21719) Homo sapiens
• MYL11 (HGNC:29824) Homo sapiens
• MYL10 (HGNC:29825) Homo sapiens
• MYL12B (HGNC:29827) Homo sapiens
• MYL2 (HGNC:7583) Homo sapiens
• MYL5 (HGNC:7586) Homo sapiens
• rlc1 (SJAG_01665) Schizosaccharomyces japonicus
• MLC2 (YPR188C) Saccharomyces cerevisiae

References / Literature

PMID:29996109 - Quantitative Phosphoproteomics Reveals the Signaling Dynamics of Cell-Cycle Kinases in the Fission Yeast Schizosaccharomyces pombe.

Swaffer MP et al. Cell Rep 2018 Jul 10;24(2):503-514

PMID:22024167 - Actin filament severing by cofilin is more important for assembly than constriction of the cytokinetic contractile ring.

Chen Q et al. J Cell Biol 2011 Oct 31;195(3):485-98

PMID:18378776 - The Clp1/Cdc14 phosphatase contributes to the robustness of cytokinesis by association with anillin-related Mid1.

Clifford DM et al. J Cell Biol 2008 Apr 07;181(1):79-88

PMID:34250083 - Barcode sequencing and a high-throughput assay for chronological lifespan uncover ageing-associated genes in fission yeast.

Romila CA et al. Microb Cell 2021 Jul 05;8(7):146-160

GO_REF:0000002 - Comments

PMID:31626996 - Multiplexed proteome profiling of carbon source perturbations in two yeast species with SL-SP3-TMT.

Paulo JA et al. J Proteomics 2020 Jan 06;210:103531

PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.

Marguerat S et al. Cell 2012 Oct 26;151(3):671-83

PMID:35108037 - Fission yeast paxillin contains two Cdc15 binding motifs for robust recruitment to the cytokinetic ring.

Snider CE et al. Mol Biol Cell 2022 Apr 01;33(4):br4

PMID:16772338 - The fission yeast Chs2 protein interacts with the type-II myosin Myo3p and is required for the integrity of the actomyosin ring.

Martín-García R et al. J Cell Sci 2006 Jul 01;119(Pt 13):2768-79

PMID:25977474 - A role for F-BAR protein Rga7p during cytokinesis in S. pombe.

Arasada R et al. J Cell Sci 2015 Jul 01;128(13):2259-68

PMID:22174761 - Roles of the DYRK kinase Pom2 in cytokinesis, mitochondrial morphology, and sporulation in fission yeast.

Wu P et al. PLoS One 2011;6(12):e28000

PMID:25720772 - Quantitative phosphoproteomics reveals pathways for coordination of cell growth and division by the conserved fission yeast kinase pom1.

Kettenbach AN et al. Mol Cell Proteomics 2015 May;14(5):1275-87

PMID:26941334 - Unique spatiotemporal activation pattern of Cdc42 by Gef1 and Scd1 promotes different events during cytokinesis.

Wei B et al. Mol Biol Cell 2016 Apr 15;27(8):1235-45

PMID:25451933 - Aip1 promotes actin filament severing by cofilin and regulates constriction of the cytokinetic contractile ring.

Chen Q et al. J Biol Chem 2015 Jan 23;290(4):2289-300

PMID:24763107 - Absolute proteome and phosphoproteome dynamics during the cell cycle of Schizosaccharomyces pombe (Fission Yeast).

Carpy A et al. Mol Cell Proteomics 2014 Aug;13(8):1925-36

PMID:18495844 - Interphase microtubule bundles use global cell shape to guide spindle alignment in fission yeast.

Daga RR et al. J Cell Sci 2008 Jun 15;121(Pt 12):1973-80

PMID:25688133 - The F-BAR Cdc15 promotes contractile ring formation through the direct recruitment of the formin Cdc12.

Willet AH et al. J Cell Biol 2015 Feb 16;208(4):391-9

PMID:33823663 - A TOR (target of rapamycin) and nutritional phosphoproteome of fission yeast reveals novel targets in networks conserved in humans.

Halova L et al. Open Biol 2021 Apr;11(4):200405

PMID:19570908 - Regulation of fission yeast myosin-II function and contractile ring dynamics by regulatory light-chain and heavy-chain phosphorylation.

Sladewski TE et al. Mol Biol Cell 2009 Sep;20(17):3941-52

PMID:16615890 - Meiotic proteins bqt1 and bqt2 tether telomeres to form the bouquet arrangement of chromosomes.

Chikashige Y et al. Cell 2006 Apr 07;125(1):59-69

PMID:22427686 - Characterization of Mid1 domains for targeting and scaffolding in fission yeast cytokinesis.

Lee IJ et al. J Cell Sci 2012 Jun 15;125(Pt 12):2973-85

PMID:25664996 - Synthetic polyamines: new compounds specific to actin dynamics for mammalian cell and fission yeast.

Riveline D et al. Bioarchitecture 2014;4(4-5):144-8

PMID:27918601 - Identifying genes required for respiratory growth of fission yeast.

Malecki M et al. Wellcome Open Res 2016;1:12

PMID:12791993 - Equatorial retention of the contractile actin ring by microtubules during cytokinesis.

Pardo M et al. Science 2003 Jun 06;300(5625):1569-74

PMID:15743909 - Profilin-mediated competition between capping protein and formin Cdc12p during cytokinesis in fission yeast.

Kovar DR et al. Mol Biol Cell 2005 May;16(5):2313-24

PMID:19075108 - Assembly of normal actomyosin rings in the absence of Mid1p and cortical nodes in fission yeast.

Huang Y et al. J Cell Biol 2008 Dec 15;183(6):979-88

PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.

Hayles J et al. Open Biol 2013 May 22;3(5):130053

PMID:14602073 - Spatial and temporal pathway for assembly and constriction of the contractile ring in fission yeast cytokinesis.

Wu JQ et al. Dev Cell 2003 Nov;5(5):723-34

PMID:30709916 - A Cdc42 GEF, Gef1, through endocytosis organizes F-BAR Cdc15 along the actomyosin ring and promotes concentric furrowing.

Onwubiko UN et al. J Cell Sci 2019 Feb 28;132(5)

PMID:23950735 - Global analysis of fission yeast mating genes reveals new autophagy factors.

Sun LL et al. PLoS Genet 2013;9(8):e1003715

PMID:36749320 - Multiple polarity kinases inhibit phase separation of F-BAR protein Cdc15 and antagonize cytokinetic ring assembly in fission yeast.

Bhattacharjee R et al. Elife 2023 Feb 07;12

PMID:19713940 - Fission yeast IQGAP arranges actin filaments into the cytokinetic contractile ring.

Takaine M et al. EMBO J 2009 Oct 21;28(20):3117-31

PMID:11056543 - Type II myosin regulatory light chain relieves auto-inhibition of myosin-heavy-chain function.

Naqvi NI et al. Nat Cell Biol 2000 Nov;2(11):855-8

PMID:18184749 - The actomyosin ring recruits early secretory compartments to the division site in fission yeast.

Vjestica A et al. Mol Biol Cell 2008 Mar;19(3):1125-38

PMID:25452419 - Parallel profiling of fission yeast deletion mutants for proliferation and for lifespan during long-term quiescence.

Sideri T et al. G3 (Bethesda) 2014 Dec 01;5(1):145-55

PMID:21376595 - IQGAP-related Rng2p organizes cortical nodes and ensures position of cell division in fission yeast.

Padmanabhan A et al. Curr Biol 2011 Mar 22;21(6):467-72

PMID:11069761 - The S. pombe rlc1 gene encodes a putative myosin regulatory light chain that binds the type II myosins myo3p and myo2p.

Le Goff X et al. J Cell Sci 2000 Dec;113 Pt 23:4157-63

PMID:15504913 - UCS protein Rng3p activates actin filament gliding by fission yeast myosin-II.

Lord M et al. J Cell Biol 2004 Oct 25;167(2):315-25

PMID:18272786 - Pxl1p, a paxillin-related protein, stabilizes the actomyosin ring during cytokinesis in fission yeast.

Ge W et al. Mol Biol Cell 2008 Apr;19(4):1680-92

PMID:16864655 - Assembly of the cytokinetic contractile ring from a broad band of nodes in fission yeast.

Wu JQ et al. J Cell Biol 2006 Jul 31;174(3):391-402

PMID:24115772 - SIN-dependent phosphoinhibition of formin multimerization controls fission yeast cytokinesis.

Bohnert KA et al. Genes Dev 2013 Oct 01;27(19):2164-77

PMID:24127216 - The formins Cdc12 and For3 cooperate during contractile ring assembly in cytokinesis.

Coffman VC et al. J Cell Biol 2013 Oct 14;203(1):101-14

PMID:19029336 - Schizosaccharomyces pombe Pak-related protein, Pak1p/Orb2p, phosphorylates myosin regulatory light chain to inhibit cytokinesis.

Loo TH et al. J Cell Biol 2008 Dec 01;183(5):785-93

PMID:28808035 - Fission yeast myosin Myo2 is down-regulated in actin affinity by light chain phosphorylation.

Pollard LW et al. Proc Natl Acad Sci U S A 2017 Aug 29;114(35):E7236-E7244

PMID:31657618 - A Genome-Wide Screen for Wortmannin-Resistant Mutants in Schizosaccharomyces pombe: The Phosphorylation-Impaired Mutants Are Resistant to Signaling Defect.

Yılmazer M et al. DNA Cell Biol 2019 Dec;38(12):1427-1436

PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.

Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623

PMID:33109728 - High-Throughput Identification of Nuclear Envelope Protein Interactions in Schizosaccharomyces pombe Using an Arrayed Membrane Yeast-Two Hybrid Library.

Varberg JM et al. G3 (Bethesda) 2020 Dec 03;10(12):4649-4663

PMID:37787768 - Broad functional profiling of fission yeast proteins using phenomics and machine learning.

Rodríguez-López M et al. Elife 2023 Oct 03;12

PMID:28914606 - Nanoscale architecture of the Schizosaccharomyces pombe contractile ring.

McDonald NA et al. Elife 2017 Sep 15;6

PMID:28281664 - Genetic interactions and functional analyses of the fission yeast gsk3 and amk2 single and double mutants defective in TORC1-dependent processes.

Rallis C et al. Sci Rep 2017 Mar 10;7:44257

PMID:39705284 - Proteomic and phosphoproteomic analyses reveal that TORC1 is reactivated by pheromone signaling during sexual reproduction in fission yeast.

Bérard M et al. PLoS Biol 2024 Dec 20;22(12):e3002963

PMID:22918943 - Anillin-related protein Mid1p coordinates the assembly of the cytokinetic contractile ring in fission yeast.

Saha S et al. Mol Biol Cell 2012 Oct;23(20):3982-92

PMID:30853434 - NDR Kinase Sid2 Drives Anillin-like Mid1 from the Membrane to Promote Cytokinesis and Medial Division Site Placement.

Willet AH et al. Curr Biol 2019 Mar 18;29(6):1055-1063.e2

PMID:16687577 - Cell cycle-dependent roles for the FCH-domain protein Cdc15p in formation of the actomyosin ring in Schizosaccharomyces pombe.

Wachtler V et al. Mol Biol Cell 2006 Jul;17(7):3254-66

PMID:24798735 - The novel proteins Rng8 and Rng9 regulate the myosin-V Myo51 during fission yeast cytokinesis.

Wang N et al. J Cell Biol 2014 May 12;205(3):357-75

PMID:26771498 - A Proteome-wide Fission Yeast Interactome Reveals Network Evolution Principles from Yeasts to Human.

Vo TV et al. Cell 2016 Jan 14;164(1-2):310-323

PMID:21712547 - Mitotic substrates of the kinase aurora with roles in chromatin regulation identified through quantitative phosphoproteomics of fission yeast.

Koch A et al. Sci Signal 2011 Jun 28;4(179):rs6

PMID:16224022 - Counting cytokinesis proteins globally and locally in fission yeast.

Wu JQ et al. Science 2005 Oct 14;310(5746):310-4

PMID:21422229 - Assembly and architecture of precursor nodes during fission yeast cytokinesis.

Laporte D et al. J Cell Biol 2011 Mar 21;192(6):1005-21

PMID:31509478 - The intrinsically disordered region of the cytokinetic F-BAR protein Cdc15 performs a unique essential function in maintenance of cytokinetic ring integrity.

Mangione MC et al. Mol Biol Cell 2019 Oct 15;30(22):2790-2801

PMID:32142608 - Mutations in a Single Signaling Pathway Allow Cell Growth in Heavy Water.

Kampmeyer C et al. ACS Synth Biol 2020 Apr 17;9(4):733-748

PMID:20603077 - Dephosphorylation of F-BAR protein Cdc15 modulates its conformation and stimulates its scaffolding activity at the cell division site.

Roberts-Galbraith RH et al. Mol Cell 2010 Jul 09;39(1):86-99

PMID:16823372 - ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe.

Matsuyama A et al. Nat Biotechnol 2006 Jul;24(7):841-7

PMID:32101745 - Dense Transposon Integration Reveals Essential Cleavage and Polyadenylation Factors Promote Heterochromatin Formation.

Lee SY et al. Cell Rep 2020 Feb 25;30(8):2686-2698.e8

GO_REF:0000033 - Annotation inferences using phylogenetic trees

PMID:19139265 - The SH3 domains of two PCH family members cooperate in assembly of the Schizosaccharomyces pombe contractile ring.

Roberts-Galbraith RH et al. J Cell Biol 2009 Jan 12;184(1):113-27

PMID:20110347 - Tropomyosin and myosin-II cellular levels promote actomyosin ring assembly in fission yeast.

Stark BC et al. Mol Biol Cell 2010 Mar 15;21(6):989-1000

PB_REF:0000006 - Disease associations from Monarch via human-pombe orthologs

PMID:18256290 - Schizosaccharomyces pombe Pxl1 is a paxillin homologue that modulates Rho1 activity and participates in cytokinesis.

Pinar M et al. Mol Biol Cell 2008 Apr;19(4):1727-38

PMID:25483073 - Chromosome segregation and organization are targets of 5'-Fluorouracil in eukaryotic cells.

Mojardín L et al. Cell Cycle 2015;14(2):206-18

PMID:30726745 - Fission Yeast NDR/LATS Kinase Orb6 Regulates Exocytosis via Phosphorylation of the Exocyst Complex.

Tay YD et al. Cell Rep 2019 Feb 05;26(6):1654-1667.e7

PMID:28357272 - A central role for TOR signalling in a yeast model for juvenile CLN3 disease.

Bond ME et al. Microb Cell 2015 Nov 11;2(12):466-480

PMID:19279143 - Role of the RNA-binding protein Nrd1 and Pmk1 mitogen-activated protein kinase in the regulation of myosin mRNA stability in fission yeast.

Satoh R et al. Mol Biol Cell 2009 May;20(9):2473-85

PMID:21511999 - Comparative functional genomics of the fission yeasts.

Rhind N et al. Science 2011 May 20;332(6032):930-6

PMID:20434336 - The cortical ER network limits the permissive zone for actomyosin ring assembly.

Zhang D et al. Curr Biol 2010 Jun 08;20(11):1029-34