protein coding gene - rad26 (SPAC9E9.08) - ATRIP, ATR checkpoint kinase regulatory subunit Rad26

Gene summary

Standard name

rad26

Systematic ID

SPAC9E9.08

Product

ATRIP, ATR checkpoint kinase regulatory subunit Rad26

Organism

Schizosaccharomyces pombe (fission yeast)

UniProt ID

P36632

ORFeome ID

27/27A05

Characterisation status

biological role published

Feature type

mRNA gene

Genomic location

chromosome I: 4450605..4453369 forward strand

Annotation

Protein features

Orthologs

• ATRIP (HGNC:33499) Homo sapiens
• rad26 (SJAG_00429) Schizosaccharomyces japonicus
• LCD1 (YDR499W) Saccharomyces cerevisiae

References / Literature

PMID:17531813 - Cdc18 enforces long-term maintenance of the S phase checkpoint by anchoring the Rad3-Rad26 complex to chromatin.

Hermand D et al. Mol Cell 2007 May 25;26(4):553-63

PMID:11073995 - Rdp1, a novel zinc finger protein, regulates the DNA damage response of rhp51(+) from Schizosaccharomyces pombe.

Shim YS et al. Mol Cell Biol 2000 Dec;20(23):8958-68

PMID:19205745 - Redundant roles of Srs2 helicase and replication checkpoint in survival and rDNA maintenance in Schizosaccharomyces pombe.

Yasuhira S Mol Genet Genomics 2009 May;281(5):497-509

PMID:10430579 - Genetic control of telomere integrity in Schizosaccharomyces pombe: rad3(+) and tel1(+) are parts of two regulatory networks independent of the downstream protein kinases chk1(+) and cds1(+).

Matsuura A et al. Genetics 1999 Aug;152(4):1501-12

PMID:15173168 - Cds1 phosphorylation by Rad3-Rad26 kinase is mediated by forkhead-associated domain interaction with Mrc1.

Tanaka K et al. J Biol Chem 2004 Jul 30;279(31):32079-86

PMID:23628481 - A proteome-wide visual screen identifies fission yeast proteins localizing to DNA double-strand breaks.

Yu Y et al. DNA Repair (Amst) 2013 Jun 01;12(6):433-43

PMID:12839619 - High dosage Rhp51 suppression of the MMS sensitivity of DNA structure checkpoint mutants reveals a relationship between Crb2 and Rhp51.

Smeets MF et al. Genes Cells 2003 Jul;8(7):573-86

PMID:9450932 - S-phase-specific activation of Cds1 kinase defines a subpathway of the checkpoint response in Schizosaccharomyces pombe.

Lindsay HD et al. Genes Dev 1998 Feb 01;12(3):382-95

PMID:16930478 - The fission yeast DNA structure checkpoint protein Rad26ATRIP/LCD1/UVSD accumulates in the cytoplasm following microtubule destabilization.

Baschal EE et al. BMC Cell Biol 2006 Aug 24;7:32

PMID:41729970 - The RPA-binding domain and the KKRK motif in Rad26ATRIP cooperate at the perturbed DNA replication fork for initiating checkpoint signalling.

Xu YJ et al. PLoS Genet 2026 Feb 23;22(2):e1012052

PMID:16823372 - ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe.

Matsuyama A et al. Nat Biotechnol 2006 Jul;24(7):841-7

PMID:20140190 - A kinase-independent role for the Rad3(ATR)-Rad26(ATRIP) complex in recruitment of Tel1(ATM) to telomeres in fission yeast.

Subramanian L et al. PLoS Genet 2010 Feb 05;6(2):e1000839

PMID:9563836 - Mutant PCNA alleles are associated with cdc phenotypes and sensitivity to DNA damage in fission yeast.

Arroyo MP et al. Mol Gen Genet 1998 Mar;257(5):505-18

PMID:12697061 - Fission yeast Rad26 responds to DNA damage independently of Rad3.

Wolkow TD et al. BMC Genet 2003 Apr 03;4:6

PMID:16723501 - Rad4TopBP1, a scaffold protein, plays separate roles in DNA damage and replication checkpoints and DNA replication.

Taricani L et al. Mol Biol Cell 2006 Aug;17(8):3456-68

PMID:12514100 - Checkpoint activation regulates mutagenic translesion synthesis.

Kai M et al. Genes Dev 2003 Jan 01;17(1):64-76

PMID:20375067 - Fission yeast Rad26ATRIP delays spindle-pole-body separation following interphase microtubule damage.

Herring M et al. J Cell Sci 2010 May 01;123(Pt 9):1537-45

PMID:10648611 - Characterization of Schizosaccharomyces pombe Hus1: a PCNA-related protein that associates with Rad1 and Rad9.

Caspari T et al. Mol Cell Biol 2000 Feb;20(4):1254-62

PMID:15229228 - Homo-oligomerization is the essential function of the tandem BRCT domains in the checkpoint protein Crb2.

Du LL et al. J Biol Chem 2004 Sep 10;279(37):38409-14

PMID:28281664 - Genetic interactions and functional analyses of the fission yeast gsk3 and amk2 single and double mutants defective in TORC1-dependent processes.

Rallis C et al. Sci Rep 2017 Mar 10;7:44257

PMID:12196391 - Telomere binding of checkpoint sensor and DNA repair proteins contributes to maintenance of functional fission yeast telomeres.

Nakamura TM et al. Genetics 2002 Aug;161(4):1437-52

PMID:21511999 - Comparative functional genomics of the fission yeasts.

Rhind N et al. Science 2011 May 20;332(6032):930-6

PMID:25795664 - Genetic Interaction Landscape Reveals Critical Requirements for Schizosaccharomyces pombe Brc1 in DNA Damage Response Mutants.

Sánchez A et al. G3 (Bethesda) 2015 Mar 19;5(5):953-62

PMID:32034465 - Genetic investigation of formaldehyde-induced DNA damage response in Schizosaccharomyces pombe.

Anandarajan V et al. Curr Genet 2020 Jun;66(3):593-605

PMID:39476757 - Characterization of Ksg1 protein kinase-dependent phosphoproteome in the fission yeast S. pombe.

Cipak L et al. Biochem Biophys Res Commun 2024 Oct 25;736:150895

PMID:11809834 - Fission yeast F-box protein Pof3 is required for genome integrity and telomere function.

Katayama S et al. Mol Biol Cell 2002 Jan;13(1):211-24

PMID:22681890 - Hierarchical modularity and the evolution of genetic interactomes across species.

Ryan CJ et al. Mol Cell 2012 Jun 08;46(5):691-704

PMID:16371652 - Production of reactive oxygen species in response to replication stress and inappropriate mitosis in fission yeast.

Marchetti MA et al. J Cell Sci 2006 Jan 01;119(Pt 1):124-31

PMID:19264558 - Screening a genome-wide S. pombe deletion library identifies novel genes and pathways involved in genome stability maintenance.

Deshpande GP et al. DNA Repair (Amst) 2009 May 01;8(5):672-9

PMID:23050226 - A genetic screen to discover pathways affecting cohesin function in Schizosaccharomyces pombe identifies chromatin effectors.

Chen Z et al. G3 (Bethesda) 2012 Oct;2(10):1161-8

PMID:24957674 - Yeast X-chromosome-associated protein 5 (Xap5) functions with H2A.Z to suppress aberrant transcripts.

Anver S et al. EMBO Rep 2014 Aug;15(8):894-902

PMID:26771498 - A Proteome-wide Fission Yeast Interactome Reveals Network Evolution Principles from Yeasts to Human.

Vo TV et al. Cell 2016 Jan 14;164(1-2):310-323

PMID:7768995 - The Schizosaccharomyces pombe hus5 gene encodes a ubiquitin conjugating enzyme required for normal mitosis.

al-Khodairy F et al. J Cell Sci 1995 Feb;108 ( Pt 2):475-86

PMID:9154809 - A novel mutant allele of Schizosaccharomyces pombe rad26 defective in monitoring S-phase progression to prevent premature mitosis.

Uchiyama M et al. Mol Cell Biol 1997 Jun;17(6):3103-15

PMID:24623809 - The DNA damage checkpoint pathway promotes extensive resection and nucleotide synthesis to facilitate homologous recombination repair and genome stability in fission yeast.

Blaikley EJ et al. Nucleic Acids Res 2014 May;42(9):5644-56

PMID:18378696 - Molecular characterization of the role of the Schizosaccharomyces pombe nip1+/ctp1+ gene in DNA double-strand break repair in association with the Mre11-Rad50-Nbs1 complex.

Akamatsu Y et al. Mol Cell Biol 2008 Jun;28(11):3639-51

PMID:17264117 - Caf1 regulates translocation of ribonucleotide reductase by releasing nucleoplasmic Spd1-Suc22 assembly.

Takahashi S et al. Nucleic Acids Res 2007;35(4):1187-97

PMID:22645654 - Opposing role of condensin hinge against replication protein A in mitosis and interphase through promoting DNA annealing.

Akai Y et al. Open Biol 2011 Dec;1(4):110023

PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.

Hayles J et al. Open Biol 2013 May 22;3(5):130053

PMID:12589755 - The fission yeast spSet1p is a histone H3-K4 methyltransferase that functions in telomere maintenance and DNA repair in an ATM kinase Rad3-dependent pathway.

Kanoh J et al. J Mol Biol 2003 Feb 28;326(4):1081-94

PMID:36793083 - The SAGA histone acetyltransferase module targets SMC5/6 to specific genes.

Mahrik L et al. Epigenetics Chromatin 2023 Feb 16;16(1):6

PMID:17690116 - Cdc18/CDC6 activates the Rad3-dependent checkpoint in the fission yeast.

Fersht N et al. Nucleic Acids Res 2007;35(16):5323-37

PMID:18931302 - Significant conservation of synthetic lethal genetic interaction networks between distantly related eukaryotes.

Dixon SJ et al. Proc Natl Acad Sci U S A 2008 Oct 28;105(43):16653-8

PMID:9487130 - Regulation of telomere length by checkpoint genes in Schizosaccharomyces pombe.

Dahlen M et al. Mol Biol Cell 1998 Mar;9(3):611-21

PMID:15643072 - Mcl1p is a polymerase alpha replication accessory factor important for S-phase DNA damage survival.

Williams DR et al. Eukaryot Cell 2005 Jan;4(1):166-77

PMID:15340008 - Requirement for Schizosaccharomyces pombe Top3 in the maintenance of chromosome integrity.

Win TZ et al. J Cell Sci 2004 Sep 15;117(Pt 20):4769-78

PMID:30726745 - Fission Yeast NDR/LATS Kinase Orb6 Regulates Exocytosis via Phosphorylation of the Exocyst Complex.

Tay YD et al. Cell Rep 2019 Feb 05;26(6):1654-1667.e7

PMID:31332096 - A tel2 Mutation That Destabilizes the Tel2-Tti1-Tti2 Complex Eliminates Rad3 ATR Kinase Signaling in the DNA Replication Checkpoint and Leads to Telomere Shortening in Fission Yeast.

Xu YJ et al. Mol Cell Biol 2019 Oct 15;39(20)

PMID:18818364 - Conservation and rewiring of functional modules revealed by an epistasis map in fission yeast.

Roguev A et al. Science 2008 Oct 17;322(5900):405-10

PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.

Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623

PMID:12724426 - Role for the fission yeast RecQ helicase in DNA repair in G2.

Laursen LV et al. Mol Cell Biol 2003 May;23(10):3692-705

PMID:20505337 - Roles of the checkpoint sensor clamp Rad9-Rad1-Hus1 (911)-complex and the clamp loaders Rad17-RFC and Ctf18-RFC in Schizosaccharomyces pombe telomere maintenance.

Khair L et al. Cell Cycle 2010 Jun 01;9(11):2237-48

PMID:21561865 - The phosphorylation network for efficient activation of the DNA replication checkpoint in fission yeast.

Yue M et al. J Biol Chem 2011 Jul 01;286(26):22864-74

PMID:37787768 - Broad functional profiling of fission yeast proteins using phenomics and machine learning.

Rodríguez-López M et al. Elife 2023 Oct 03;12

PMID:18667534 - Activation of the DNA damage checkpoint in mutants defective in DNA replication initiation.

Yin L et al. Mol Biol Cell 2008 Oct;19(10):4374-82

PMID:15498101 - Germinating fission yeast spores delay in G1 in response to UV irradiation.

Nilssen EA et al. BMC Cell Biol 2004 Oct 21;5(1):40

PMID:27687866 - Genetic controls of DNA damage avoidance in response to acetaldehyde in fission yeast.

Noguchi C et al. Cell Cycle 2017 Jan 02;16(1):45-58

PMID:30148840 - Tdp1 processes chromate-induced single-strand DNA breaks that collapse replication forks.

Ganguly A et al. PLoS Genet 2018 Aug;14(8):e1007595

PMID:10559981 - A Rad3-Rad26 complex responds to DNA damage independently of other checkpoint proteins.

Edwards RJ et al. Nat Cell Biol 1999 Nov;1(7):393-8

PMID:10683155 - A novel mutant allele of the chromatin-bound fission yeast checkpoint protein Rad17 separates the DNA structure checkpoints.

Griffiths D et al. J Cell Sci 2000 Mar;113 ( Pt 6):1075-88

PMID:10954610 - The stress-activated MAP kinase Sty1/Spc1 and a 3'-regulatory element mediate UV-induced expression of the uvi15(+) gene at the post-transcriptional level.

Kim M et al. Nucleic Acids Res 2000 Sep 01;28(17):3392-402

PMID:12062100 - Cid13 is a cytoplasmic poly(A) polymerase that regulates ribonucleotide reductase mRNA.

Saitoh S et al. Cell 2002 May 31;109(5):563-73

PMID:9693370 - Mutational effect of fission yeast polalpha on cell cycle events.

Bhaumik D et al. Mol Biol Cell 1998 Aug;9(8):2107-23

PMID:12455694 - mcl1+, the Schizosaccharomyces pombe homologue of CTF4, is important for chromosome replication, cohesion, and segregation.

Williams DR et al. Eukaryot Cell 2002 Oct;1(5):758-73

PMID:12186947 - A novel chk1-dependent G1/M checkpoint in fission yeast.

Synnes M et al. J Cell Sci 2002 Sep 15;115(Pt 18):3609-18

PMID:12871901 - The Schizosaccharomyces pombe cdt2(+) gene, a target of G1-S phase-specific transcription factor complex DSC1, is required for mitotic and premeiotic DNA replication.

Yoshida SH et al. Genetics 2003 Jul;164(3):881-93

PMID:8846774 - Fission yeast rad17: a homologue of budding yeast RAD24 that shares regions of sequence similarity with DNA polymerase accessory proteins.

Griffiths DJ et al. EMBO J 1995 Dec 01;14(23):5812-23

PMID:15548596 - Analysis of mutant phenotypes and splicing defects demonstrates functional collaboration between the large and small subunits of the essential splicing factor U2AF in vivo.

Webb CJ et al. Mol Biol Cell 2005 Feb;16(2):584-96

PMID:19214192 - Differential arrival of leading and lagging strand DNA polymerases at fission yeast telomeres.

Moser BA et al. EMBO J 2009 Apr 08;28(7):810-20

PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.

Marguerat S et al. Cell 2012 Oct 26;151(3):671-83

PMID:24663817 - Rad4 mainly functions in Chk1-mediated DNA damage checkpoint pathway as a scaffold protein in the fission yeast Schizosaccharomyces pombe.

Yue M et al. PLoS One 2014;9(3):e92936

PMID:24815688 - Does a shift to limited glucose activate checkpoint control in fission yeast?

Saitoh S et al. FEBS Lett 2014 Aug 01;588(15):2373-8

PMID:9524127 - Hus1p, a conserved fission yeast checkpoint protein, interacts with Rad1p and is phosphorylated in response to DNA damage.

Kostrub CF et al. EMBO J 1998 Apr 01;17(7):2055-66

PMID:9891047 - Mutator phenotype induced by aberrant replication.

Liu VF et al. Mol Cell Biol 1999 Feb;19(2):1126-35

PMID:12032307 - A single unbranched S-phase DNA damage and replication fork blockage checkpoint pathway.

Marchetti MA et al. Proc Natl Acad Sci U S A 2002 May 28;99(11):7472-7

PMID:9153313 - Isolation and characterization of the Schizosaccharomyces pombe rhp9 gene: a gene required for the DNA damage checkpoint but not the replication checkpoint.

Willson J et al. Nucleic Acids Res 1997 Jun 01;25(11):2138-46

PMID:12865439 - Delineating the position of rad4+/cut5+ within the DNA-structure checkpoint pathways in Schizosaccharomyces pombe.

Harris S et al. J Cell Sci 2003 Sep 01;116(Pt 17):3519-29

PMID:28441348 - Deoxynucleoside Salvage in Fission Yeast Allows Rescue of Ribonucleotide Reductase Deficiency but Not Spd1-Mediated Inhibition of Replication.

Fleck O et al. Genes (Basel) 2017 Apr 25;8(5)

PMID:29259000 - Genes Important for Schizosaccharomyces pombe Meiosis Identified Through a Functional Genomics Screen.

Blyth J et al. Genetics 2018 Feb;208(2):589-603

PMID:20967229 - Checkpoint-dependent and -independent roles of Swi3 in replication fork recovery and sister chromatid cohesion in fission yeast.

Rapp JB et al. PLoS One 2010 Oct 12;5(10):e13379

PMID:12930957 - Nuclear factories for signalling and repairing DNA double strand breaks in living fission yeast.

Meister P et al. Nucleic Acids Res 2003 Sep 01;31(17):5064-73

PMID:11027257 - Analysis of fission yeast primase defines the checkpoint responses to aberrant S phase initiation.

Tan S et al. Mol Cell Biol 2000 Nov;20(21):7853-66