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protein coding gene - cyk3 (SPAC9G1.06c) - Nebulin-family actin filament anchoring protein Cyk3

Gene summary

Standard name
cyk3
Systematic ID
SPAC9G1.06c
Product
Nebulin-family actin filament anchoring protein Cyk3
Organism
Schizosaccharomyces pombe (fission yeast)
UniProt ID
O14302
ORFeome ID
47/47B08
Characterisation status
biological role published
Feature type
mRNA gene
Genomic location
chromosome I: 1976754..1982149 reverse strand

Annotation

Disease association

MONDO:0030281 - arthrogryposis multiplex congenita 6

References:

MONDO:0018958 - nemaline myopathy

References:

MONDO:0009725 - nemaline myopathy 2

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GO biological process

GO:1902404 - mitotic actomyosin contractile ring contraction

References:

GO:0140278 - mitotic division septum assembly

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GO cellular component

GO:0005938 - cell cortex

References:

GO:0051285 - cell cortex of cell tip

References:

GO:0140472 - cell cortex of non-growing cell tip

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GO:0032153 - cell division site

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GO:0051286 - cell tip

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GO:0000935 - division septum

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GO:0110085 - mitotic actomyosin contractile ring

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GO:0120105 - mitotic actomyosin contractile ring, intermediate layer

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Modification

MOD:00046 - O-phospho-L-serine

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MOD:00047 - O-phospho-L-threonine

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MOD:00696 - phosphorylated residue

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MOD:01148 - ubiquitinylated lysine

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Multi-locus phenotype

FYPO:0004481 - abolished cell population growth at high temperature

References:

Genotypes:

FYPO:0007774 - increased invasive hyphal growth

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Genotypes:

FYPO:0003532 - increased monopolar index

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Genotypes:

FYPO:0004495 - inviable branched, swollen, elongated, multiseptate vegetative cell

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Genotypes:

FYPO:0000674 - normal cell population growth at high temperature

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Genotypes:

Qualitative gene expression

PomGeneEx:0000019 - protein level decreased

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PomGeneEx:0000018 - protein level increased

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Quantitative gene expression

PBO:0006310 - protein level

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PBO:0011963 - RNA level

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Single locus phenotype

FYPO:0000151 - abnormal meiotic chromosome segregation

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Genotypes:

FYPO:0001018 - abolished NETO

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Genotypes:

FYPO:0009073 - decreased cell population growth on lysine nitrogen source

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Genotypes:

FYPO:0004653 - delayed onset of actomyosin contractile ring contraction

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Genotypes:

FYPO:0006559 - delayed onset of mitotic spindle disassembly

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Genotypes:

FYPO:0004167 - increased cell population growth on glycerol carbon source

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Genotypes:

FYPO:0000651 - increased duration of septum assembly

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Genotypes:

FYPO:0007774 - increased invasive hyphal growth

References:

Genotypes:

FYPO:0003532 - increased monopolar index

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Genotypes:

FYPO:0003776 - increased pseudohyphal growth

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Genotypes:

FYPO:0000650 - increased septation index

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Genotypes:

FYPO:0004557 - increased vegetative cell population growth

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Genotypes:

FYPO:0001309 - increased viability in stationary phase

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Genotypes:

FYPO:0004076 - inviable after spore germination, single or double cell division, elongated multiseptate cell

References:

Genotypes:

FYPO:0000674 - normal cell population growth at high temperature

References:

Genotypes:

FYPO:0006187 - normal rate of actomyosin contractile ring assembly

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Genotypes:

FYPO:0004895 - normal rate of actomyosin contractile ring contraction

References:

Genotypes:

FYPO:0003383 - resistance to tert-butyl hydroperoxide

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Genotypes:

FYPO:0007933 - sensitive to 2,2′-dipyridyl

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Genotypes:

FYPO:0001097 - sensitive to amitrole

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Genotypes:

FYPO:0009067 - sensitive to amorolfine

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Genotypes:

FYPO:0007921 - sensitive to benzamidine

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Genotypes:

FYPO:0000096 - sensitive to cadmium

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Genotypes:

FYPO:0000104 - sensitive to cycloheximide

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Genotypes:

FYPO:0000799 - sensitive to diamide

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Genotypes:

FYPO:0007931 - sensitive to egtazic acid

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Genotypes:

FYPO:0007928 - sensitive to ethylenediaminetetraacetic acid

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Genotypes:

FYPO:0000087 - sensitive to hydrogen peroxide

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Genotypes:

FYPO:0009086 - sensitive to lithium chloride and sodium dodecyl sulfate

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Genotypes:

FYPO:0000089 - sensitive to methyl methanesulfonate

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Genotypes:

FYPO:0007924 - sensitive to potassium chloride and sodium dodecyl sulfate

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Genotypes:

FYPO:0009090 - sensitive to sodium chloride and sodium dodecyl sulfate

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Genotypes:

FYPO:0000115 - sensitive to valproic acid

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Genotypes:

FYPO:0000021 - spheroid vegetative cell

References:

Genotypes:

FYPO:0002060 - viable vegetative cell population

References:

Genotypes:

FYPO:0001510 - viable vegetative cell, abnormal cell shape, normal cell size

References:

Genotypes:

Taxonomic conservation

PBO:0011065 - conserved in eukaryotes

PBO:0011064 - conserved in fungi

PBO:0011069 - conserved in metazoa

PBO:0011070 - conserved in vertebrates

PBO:0006222 - predominantly single copy (one to one)

Protein features

IDNameInterPro nameDB name
PF00018SH3_1SH3_domainPfam
PF01841Transglut_coreTransglutaminase-likePfam
PF24584Ig_CYK3_CIg_CYK3_CPfam
cd11889SH3_Cyk3p-likeCyk3_SH3CDD
PS50002SH3SH3_domainPROSITE profiles
SM00326SH3SH3_domainSMART
SM00460TGcTransglutaminase-likeSMART
G3DSA:2.30.30.40:FF:000168CATH-FunFam
G3DSA:3.10.620.30:FF:000005CATH-FunFam
G3DSA:2.30.30.40CATH-Gene3D
G3DSA:3.10.620.30CATH-Gene3D
SSF50044SH3-like_dom_sfSUPERFAMILY
SSF54001Papain-like_cys_pep_sfSUPERFAMILY
PTHR46333CAP/Cytokinesis_proteinPANTHER
mobidb-lite-Disorderdisorder_predictionMobiDB-Disorder
mobidb-lite-Low-complexitydisorder_predictionMobiDB-Low-complexity
mobidb-lite-Polardisorder_predictionMobiDB-Polar

Orthologs

References / Literature

PANTHER:PTHR11039 - Unknown title
PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.
Marguerat S et al. Cell 2012 Oct 26;151(3):671-83
PMID:25720772 - Quantitative phosphoproteomics reveals pathways for coordination of cell growth and division by the conserved fission yeast kinase pom1.
Kettenbach AN et al. Mol Cell Proteomics 2015 May;14(5):1275-87
PMID:26167880 - SR protein kinases promote splicing of nonconsensus introns.
Lipp JJ et al. Nat Struct Mol Biol 2015 Aug;22(8):611-7
PMID:27298342 - Identification of S-phase DNA damage-response targets in fission yeast reveals conservation of damage-response networks.
Willis NA et al. Proc Natl Acad Sci U S A 2016 Jun 28;113(26):E3676-85
PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.
Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623
PMID:37158439 - The fission yeast cytokinetic ring component Fic1 promotes septum formation.
Rossi AM et al. Biol Open 2023 May 15;12(5)
PMID:29249658 - Mechanisms Connecting the Conserved Protein Kinases Ssp1, Kin1, and Pom1 in Fission Yeast Cell Polarity and Division.
Lee ME et al. Curr Biol 2018 Jan 08;28(1):84-92.e4
PMID:23297348 - Comprehensive proteomics analysis reveals new substrates and regulators of the fission yeast clp1/cdc14 phosphatase.
Chen JS et al. Mol Cell Proteomics 2013 May;12(5):1074-86
PMID:28914606 - Nanoscale architecture of the Schizosaccharomyces pombe contractile ring.
McDonald NA et al. Elife 2017 Sep 15;6
PMID:22681890 - Hierarchical modularity and the evolution of genetic interactomes across species.
Ryan CJ et al. Mol Cell 2012 Jun 08;46(5):691-704
PMID:37970674 - SUMOylation regulates Lem2 function in centromere clustering and silencing.
Strachan J et al. J Cell Sci 2023 Dec 01;136(23)
PMID:34250083 - Barcode sequencing and a high-throughput assay for chronological lifespan uncover ageing-associated genes in fission yeast.
Romila CA et al. Microb Cell 2021 Jul 05;8(7):146-160
PMID:18257517 - Phosphoproteome analysis of fission yeast.
Wilson-Grady JT et al. J Proteome Res 2008 Mar;7(3):1088-97
PMID:22573890 - Fission yeast Cyk3p is a transglutaminase-like protein that participates in cytokinesis and cell morphogenesis.
Pollard LW et al. Mol Biol Cell 2012 Jul;23(13):2433-44
PMID:27984725 - CDK Substrate Phosphorylation and Ordering the Cell Cycle.
Swaffer MP et al. Cell 2016 Dec 15;167(7):1750-1761.e16
PB_REF:0000006 - Disease associations from Monarch via human-pombe orthologs
PMID:33823663 - A TOR (target of rapamycin) and nutritional phosphoproteome of fission yeast reveals novel targets in networks conserved in humans.
Halova L et al. Open Biol 2021 Apr;11(4):200405
PMID:21712547 - Mitotic substrates of the kinase aurora with roles in chromatin regulation identified through quantitative phosphoproteomics of fission yeast.
Koch A et al. Sci Signal 2011 Jun 28;4(179):rs6
PMID:29996109 - Quantitative Phosphoproteomics Reveals the Signaling Dynamics of Cell-Cycle Kinases in the Fission Yeast Schizosaccharomyces pombe.
Swaffer MP et al. Cell Rep 2018 Jul 10;24(2):503-514
PMID:37787768 - Broad functional profiling of fission yeast proteins using phenomics and machine learning.
Rodríguez-López M et al. Elife 2023 Oct 03;12
PMID:28281664 - Genetic interactions and functional analyses of the fission yeast gsk3 and amk2 single and double mutants defective in TORC1-dependent processes.
Rallis C et al. Sci Rep 2017 Mar 10;7:44257
PMID:23093943 - Cytokinesis-based constraints on polarized cell growth in fission yeast.
Bohnert KA et al. PLoS Genet 2012;8(10):e1003004
PMID:18931302 - Significant conservation of synthetic lethal genetic interaction networks between distantly related eukaryotes.
Dixon SJ et al. Proc Natl Acad Sci U S A 2008 Oct 28;105(43):16653-8
PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.
Hayles J et al. Open Biol 2013 May 22;3(5):130053
PMID:28357272 - A central role for TOR signalling in a yeast model for juvenile CLN3 disease.
Bond ME et al. Microb Cell 2015 Nov 11;2(12):466-480
PMID:34296454 - The TOR-dependent phosphoproteome and regulation of cellular protein synthesis.
Mak T et al. EMBO J 2021 Aug 16;40(16):e107911
PMID:26771498 - A Proteome-wide Fission Yeast Interactome Reveals Network Evolution Principles from Yeasts to Human.
Vo TV et al. Cell 2016 Jan 14;164(1-2):310-323
PMID:21511999 - Comparative functional genomics of the fission yeasts.
Rhind N et al. Science 2011 May 20;332(6032):930-6
PMID:20603077 - Dephosphorylation of F-BAR protein Cdc15 modulates its conformation and stimulates its scaffolding activity at the cell division site.
Roberts-Galbraith RH et al. Mol Cell 2010 Jul 09;39(1):86-99
PMID:24763107 - Absolute proteome and phosphoproteome dynamics during the cell cycle of Schizosaccharomyces pombe (Fission Yeast).
Carpy A et al. Mol Cell Proteomics 2014 Aug;13(8):1925-36
PMID:39476757 - Characterization of Ksg1 protein kinase-dependent phosphoproteome in the fission yeast S. pombe.
Cipak L et al. Biochem Biophys Res Commun 2024 Oct 25;736:150895
PMID:39705284 - Proteomic and phosphoproteomic analyses reveal that TORC1 is reactivated by pheromone signaling during sexual reproduction in fission yeast.
Bérard M et al. PLoS Biol 2024 Dec 20;22(12):e3002963
PMID:39367033 - Quantitative proteomics and phosphoproteomics profiling of meiotic divisions in the fission yeast Schizosaccharomyces pombe.
Sivakova B et al. Sci Rep 2024 Oct 04;14(1):23105
PMID:25428987 - The Cdc15 and Imp2 SH3 domains cooperatively scaffold a network of proteins that redundantly ensure efficient cell division in fission yeast.
Ren L et al. Mol Biol Cell 2015 Jan 15;26(2):256-69
PMID:30726745 - Fission Yeast NDR/LATS Kinase Orb6 Regulates Exocytosis via Phosphorylation of the Exocyst Complex.
Tay YD et al. Cell Rep 2019 Feb 05;26(6):1654-1667.e7
PMID:16823372 - ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe.
Matsuyama A et al. Nat Biotechnol 2006 Jul;24(7):841-7
PMID:29259000 - Genes Important for Schizosaccharomyces pombe Meiosis Identified Through a Functional Genomics Screen.
Blyth J et al. Genetics 2018 Feb;208(2):589-603