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protein coding gene - sid1 (SPAC9G1.09) - PAK-related GC kinase Sid1

Gene summary

Standard name
sid1
Systematic ID
SPAC9G1.09
Product
PAK-related GC kinase Sid1
Organism
Schizosaccharomyces pombe (fission yeast)
UniProt ID
O14305
ORFeome ID
23/23F12
Characterisation status
biological role published
Feature type
mRNA gene
Genomic location
chromosome I: 1985698..1991234 forward strand

Annotation

Complementation

PBO:0024828 - functionally complemented by Brassica napus BnMAP4Kalpha2

References:

GO biological process

GO:1902412 - regulation of mitotic cytokinesis

References:

GO:0031028 - septation initiation signaling

References:

GO cellular component

GO:0005829 - cytosol

References:

GO:0035974 - meiotic spindle pole body

References:

GO:0044732 - mitotic spindle pole body

References:

GO:0071958 - new mitotic spindle pole body

References:

GO:0005634 - nucleus

References:

GO molecular function

GO:0005515 - protein binding

References:

GO:0004674 - protein serine/threonine kinase activity

References:

Modification

MOD:00046 - O-phospho-L-serine

References:

MOD:00047 - O-phospho-L-threonine

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Multi-locus phenotype

FYPO:0000272 - abolished septum assembly

References:

Genotypes:

FYPO:0000082 - decreased cell population growth at high temperature

References:

Genotypes:

FYPO:0001355 - decreased vegetative cell population growth

References:

Genotypes:

FYPO:0000650 - increased septation index

References:

Genotypes:

FYPO:0002066 - inviable elongated aseptate cell

References:

Genotypes:

FYPO:0002024 - inviable elongated multinucleate aseptate vegetative cell

References:

Genotypes:

FYPO:0002061 - inviable vegetative cell population

References:

Genotypes:

FYPO:0000118 - multiseptate vegetative cell

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Genotypes:

FYPO:0000674 - normal cell population growth at high temperature

References:

Genotypes:

FYPO:0004082 - normal protein phosphorylation during mitosis

References:

Genotypes:

FYPO:0002060 - viable vegetative cell population

References:

Genotypes:

Protein features

PBO:0111845 - GCK subfamily

Quantitative gene expression

PBO:0006310 - protein level

References:

PBO:0011963 - RNA level

References:

Single locus phenotype

FYPO:0001364 - abnormal actomyosin contractile ring contraction

References:

Genotypes:

FYPO:0001972 - abnormal cell separation after cytokinesis resulting in septated cell

References:

Genotypes:

FYPO:0000059 - abnormal mitotic cell cycle

References:

Genotypes:

FYPO:0000586 - abolished ascospore wall assembly

References:

Genotypes:

FYPO:0006341 - abolished equatorial endocytosis during mitosis

References:

Genotypes:

FYPO:0001384 - abolished protein kinase activity

References:

Genotypes:

FYPO:0001880 - abolished protein localization to cell division site

References:

Genotypes:

FYPO:0002555 - abolished protein localization to medial cortex during vegetative growth

References:

Genotypes:

FYPO:0000941 - abolished protein localization to mitotic spindle pole body

References:

Genotypes:

FYPO:0006825 - abolished protein localization to mitotic spindle pole body during mitosis

References:

Genotypes:

FYPO:0000705 - abolished protein-protein interaction

References:

Genotypes:

FYPO:0000272 - abolished septum assembly

References:

Genotypes:

FYPO:0004562 - binucleate aseptate vegetative cell

References:

Genotypes:

FYPO:0002156 - binucleate monoseptate cell, with angled septum

References:

Genotypes:

FYPO:0001876 - decreased asymmetric protein localization, with protein localized to both mitotic spindle pole bodies during anaphase

References:

Genotypes:

FYPO:0000082 - decreased cell population growth at high temperature

References:

Genotypes:

FYPO:0001382 - decreased protein kinase activity

References:

Genotypes:

FYPO:0003331 - decreased protein kinase activity during mitotic interphase

References:

Genotypes:

FYPO:0002049 - elongated multinucleate aseptate vegetative cell

References:

Genotypes:

FYPO:0001253 - elongated multinucleate multiseptate vegetative cell, single septa between nuclei

References:

Genotypes:

FYPO:0000314 - inviable after spore germination with elongated germ tube

References:

Genotypes:

FYPO:0001493 - inviable elongated multinucleate vegetative cell

References:

Genotypes:

FYPO:0002061 - inviable vegetative cell population

References:

Genotypes:

FYPO:0001368 - normal actomyosin contractile ring assembly

References:

Genotypes:

FYPO:0000478 - normal meiosis

References:

Genotypes:

FYPO:0000833 - normal protein level during vegetative growth

References:

Genotypes:

FYPO:0002967 - normal protein localization to mitotic spindle pole body

References:

Genotypes:

FYPO:0007568 - normal protein localization to mitotic spindle pole body during anaphase

References:

Genotypes:

FYPO:0005709 - normal protein localization to mitotic spindle pole body during mitosis

References:

Genotypes:

FYPO:0004082 - normal protein phosphorylation during mitosis

References:

Genotypes:

FYPO:0000703 - normal protein-protein interaction

References:

Genotypes:

FYPO:0001904 - premature actomyosin contractile ring disassembly

References:

Genotypes:

FYPO:0000086 - sensitive to tacrolimus

References:

Genotypes:

Taxonomic conservation

PBO:0011065 - conserved in eukaryotes

PBO:0011071 - conserved in eukaryotes only

PBO:0011064 - conserved in fungi

PBO:0011069 - conserved in metazoa

PBO:0011070 - conserved in vertebrates

PBO:0000055 - no apparent S. cerevisiae ortholog

Protein features

IDNameInterPro nameDB name
PF00069PkinaseProt_kinase_domPFAM
cd06609STKc_MST3_likeCDD
PS00107PROTEIN_KINASE_ATPProtein_kinase_ATP_BSPROSITE_PATTERNS
PS50011PROTEIN_KINASE_DOMProt_kinase_domPROSITE_PROFILES
SM00220serkin_6Prot_kinase_domSMART
G3DSA:1.10.510.10:FF:000837FUNFAM
SSF56112Protein kinase-like (PK-like)Kinase-like_dom_sfSUPERFAMILY
G3DSA:1.10.510.10Transferase(Phosphotransferase) domain 1GENE3D
PTHR48012STERILE20-LIKE KINASE, ISOFORM B-RELATEDSTE20/SPS1-PAKPANTHER

Orthologs

References / Literature

PMID:10545452 - Drc1p/Cps1p, a 1,3-beta-glucan synthase subunit, is essential for division septum assembly in Schizosaccharomyces pombe.
Liu J et al. Genetics 1999 Nov;153(3):1193-203
PMID:12354095 - Calcineurin is implicated in the regulation of the septation initiation network in fission yeast.
Lu Y et al. Genes Cells 2002 Oct;7(10):1009-19
PMID:15265986 - The Clp1p/Flp1p phosphatase ensures completion of cytokinesis in response to minor perturbation of the cell division machinery in Schizosaccharomyces pombe.
Mishra M et al. J Cell Sci 2004 Aug 01;117(Pt 17):3897-910
PMID:15292395 - AtSGP1, AtSGP2 and MAP4K alpha are nucleolar plant proteins that can complement fission yeast mutants lacking a functional SIN pathway.
Champion A et al. J Cell Sci 2004 Aug 15;117(Pt 18):4265-75
PMID:16787941 - The Schizosaccharomyces pombe septation initiation network (SIN) is required for spore formation in meiosis.
Krapp A et al. J Cell Sci 2006 Jul 15;119(Pt 14):2882-91
PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.
Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623
PMID:20826461 - The role of Schizosaccharomyces pombe dma1 in spore formation during meiosis.
Krapp A et al. J Cell Sci 2010 Oct 01;123(Pt 19):3284-93
PMID:20980623 - A novel role of Dma1 in regulating forespore membrane assembly and sporulation in fission yeast.
Li WZ et al. Mol Biol Cell 2010 Dec;21(24):4349-60
PMID:10459013 - Sid2p, a spindle pole body kinase that regulates the onset of cytokinesis.
Sparks CA et al. J Cell Biol 1999 Aug 23;146(4):777-90
PMID:11676915 - S. pombe cdc11p, together with sid4p, provides an anchor for septation initiation network proteins on the spindle pole body.
Krapp A et al. Curr Biol 2001 Oct 16;11(20):1559-68
PMID:11952833 - The gamma-tubulin complex protein Alp4 provides a link between the metaphase checkpoint and cytokinesis in fission yeast.
Vardy L et al. Genes Cells 2002 Apr;7(4):365-73
PMID:12242222 - The Scw1 RNA-binding domain protein regulates septation and cell-wall structure in fission yeast.
Karagiannis J et al. Genetics 2002 Sep;162(1):45-58
PMID:21511999 - Comparative functional genomics of the fission yeasts.
Rhind N et al. Science 2011 May 20;332(6032):930-6
PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.
Hayles J et al. Open Biol 2013 May 22;3(5):130053
PMID:24838944 - Dma1-dependent degradation of SIN proteins during meiosis in Schizosaccharomyces pombe.
Krapp A et al. J Cell Sci 2014 Jul 15;127(Pt 14):3149-61
PMID:16141239 - Endocytosis in fission yeast is spatially associated with the actin cytoskeleton during polarised cell growth and cytokinesis.
Gachet Y et al. J Cell Sci 2005 Sep 15;118(Pt 18):4231-42
PMID:20826805 - Fission yeast germinal center (GC) kinase Ppk11 interacts with Pmo25 and plays an auxiliary role in concert with the morphogenesis Orb6 network (MOR) in cell morphogenesis.
Goshima T et al. J Biol Chem 2010 Nov 05;285(45):35196-205
PMID:21131906 - Dma1 ubiquitinates the SIN scaffold, Sid4, to impede the mitotic localization of Plo1 kinase.
Johnson AE et al. EMBO J 2011 Jan 19;30(2):341-54
PMID:38865179 - Transient PP2A SIP complex localization to mitotic SPBs for SIN inhibition is mediated solely by the Csc1 FHA domain.
Willet AH et al. Mol Biol Cell 2024 Jun 12;:mbcE24040196
PMID:17538026 - The nucleolar Net1/Cfi1-related protein Dnt1 antagonizes the septation initiation network in fission yeast.
Jin QW et al. Mol Biol Cell 2007 Aug;18(8):2924-34
PMID:17317928 - A method for Pmo25-associated kinase assay in fission yeast: the activity is dependent on two gC kinases Nak1 and Sid1.
Kume K et al. Biosci Biotechnol Biochem 2007 Feb;71(2):615-7
PMID:10775265 - The role of the sid1p kinase and cdc14p in regulating the onset of cytokinesis in fission yeast.
Guertin DA et al. EMBO J 2000 Apr 17;19(8):1803-15
PMID:24763107 - Absolute proteome and phosphoproteome dynamics during the cell cycle of Schizosaccharomyces pombe (Fission Yeast).
Carpy A et al. Mol Cell Proteomics 2014 Aug;13(8):1925-36
PMID:10769201 - The S. pombe orthologue of the S. cerevisiae mob1 gene is essential and functions in signalling the onset of septum formation.
Salimova E et al. J Cell Sci 2000 May;113 ( Pt 10):1695-704
PMID:18303049 - Chemical genetic analysis of the regulatory role of Cdc2p in the S. pombe septation initiation network.
Dischinger S et al. J Cell Sci 2008 Mar 15;121(Pt 6):843-53
PMID:9649519 - Isolation and characterization of new fission yeast cytokinesis mutants.
Balasubramanian MK et al. Genetics 1998 Jul;149(3):1265-75
PMID:18562696 - The meiosis-specific Sid2p-related protein Slk1p regulates forespore membrane assembly in fission yeast.
Yan H et al. Mol Biol Cell 2008 Sep;19(9):3676-90
PMID:12796296 - Scw1p antagonizes the septation initiation network to regulate septum formation and cell separation in the fission yeast Schizosaccharomyces pombe.
Jin QW et al. Eukaryot Cell 2003 Jun;2(3):510-20
PMID:10462526 - The S. pombe zfs1 gene is required to prevent septation if mitotic progression is inhibited.
Beltraminelli N et al. J Cell Sci 1999 Sep;112 Pt 18:3103-14
PMID:16415366 - A role for the septation initiation network in septum assembly revealed by genetic analysis of sid2-250 suppressors.
Jin QW et al. Genetics 2006 Apr;172(4):2101-12
PMID:11448769 - Fission yeast Clp1p phosphatase regulates G2/M transition and coordination of cytokinesis with cell cycle progression.
Trautmann S et al. Curr Biol 2001 Jun 26;11(12):931-40
PMID:16823372 - ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe.
Matsuyama A et al. Nat Biotechnol 2006 Jul;24(7):841-7
PMID:16325501 - The fission yeast MO25 protein functions in polar growth and cell separation.
Mendoza M et al. Eur J Cell Biol 2005 Dec;84(12):915-26
PMID:22419817 - The fission yeast septation initiation network (SIN) kinase, Sid2, is required for SIN asymmetry and regulates the SIN scaffold, Cdc11.
Feoktistova A et al. Mol Biol Cell 2012 May;23(9):1636-45
PMID:11271422 - Bypass of the requirement for cdc16p GAP function in Schizosaccharomyces pombe by mutation of the septation initiation network genes.
Fournier N et al. Arch Microbiol 2001 Jan;175(1):62-9
PMID:12479804 - Dma1 prevents mitotic exit and cytokinesis by inhibiting the septation initiation network (SIN).
Guertin DA et al. Dev Cell 2002 Dec;3(6):779-90
PMID:11384993 - Interaction between the noncatalytic region of Sid1p kinase and Cdc14p is required for full catalytic activity and localization of Sid1p.
Guertin DA et al. J Biol Chem 2001 Jul 27;276(30):28185-9
PMID:10805785 - Sid4p is required to localize components of the septation initiation pathway to the spindle pole body in fission yeast.
Chang L et al. Proc Natl Acad Sci U S A 2000 May 09;97(10):5249-54
PMID:20805322 - The mitosis-to-interphase transition is coordinated by cross talk between the SIN and MOR pathways in Schizosaccharomyces pombe.
Ray S et al. J Cell Biol 2010 Sep 06;190(5):793-805
PMID:24790095 - Characterization of the roles of Blt1p in fission yeast cytokinesis.
Goss JW et al. Mol Biol Cell 2014 Jul 01;25(13):1946-57
PMID:11514436 - Correct regulation of the septation initiation network in Schizosaccharomyces pombe requires the activities of par1 and par2.
Jiang W et al. Genetics 2001 Aug;158(4):1413-29
PMID:20876564 - Antagonistic roles of PP2A-Pab1 and Etd1 in the control of cytokinesis in fission yeast.
Lahoz A et al. Genetics 2010 Dec;186(4):1261-70
PMID:16096637 - Fission yeast MO25 protein is localized at SPB and septum and is essential for cell morphogenesis.
Kanai M et al. EMBO J 2005 Sep 07;24(17):3012-25
PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.
Marguerat S et al. Cell 2012 Oct 26;151(3):671-83
PMID:23297348 - Comprehensive proteomics analysis reveals new substrates and regulators of the fission yeast clp1/cdc14 phosphatase.
Chen JS et al. Mol Cell Proteomics 2013 May;12(5):1074-86
PMID:21712547 - Mitotic substrates of the kinase aurora with roles in chromatin regulation identified through quantitative phosphoproteomics of fission yeast.
Koch A et al. Sci Signal 2011 Jun 28;4(179):rs6
PMID:38989013 - Characterization of temperature-sensitive alleles of Schizosaccharomyces pombe septation initiation network components.
Turner LA et al. MicroPubl Biol 2024;2024
PMID:12546793 - Mitotic hyperphosphorylation of the fission yeast SIN scaffold protein cdc11p is regulated by the protein kinase cdc7p.
Krapp A et al. Curr Biol 2003 Jan 21;13(2):168-72
PMID:15537703 - The nuclear kinase Lsk1p positively regulates the septation initiation network and promotes the successful completion of cytokinesis in response to perturbation of the actomyosin ring in Schizosaccharomyces pombe.
Karagiannis J et al. Mol Biol Cell 2005 Jan;16(1):358-71