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protein coding gene - mdb1 (SPACUNK4.14) - BRCT domain protein Mdb1

Gene summary

Standard name
mdb1
Systematic ID
SPACUNK4.14
Product
BRCT domain protein Mdb1
Organism
Schizosaccharomyces pombe (fission yeast)
UniProt ID
O14079
ORFeome ID
35/35A01
Characterisation status
biological role published
Feature type
mRNA gene
Genomic location
chromosome I: 2882793..2885364 reverse strand

Annotation

GO biological process

GO:0007095 - mitotic G2 DNA damage checkpoint signaling

References:

GO cellular component

GO:1990023 - mitotic spindle midzone

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GO:0005634 - nucleus

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GO:0035861 - site of double-strand break

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GO molecular function

GO:0005515 - protein binding

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Modification

MOD:00046 - O-phospho-L-serine

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MOD:00047 - O-phospho-L-threonine

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MOD:00696 - phosphorylated residue

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MOD:01149 - sumoylated lysine

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Multi-locus phenotype

FYPO:0001164 - normal growth on glucose carbon source

References:

Genotypes:

FYPO:0000085 - sensitive to camptothecin

References:

Genotypes:

Protein features

PBO:0111779 - BRCT domain

Quantitative gene expression

PBO:0006310 - protein level

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PBO:0011963 - RNA level

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Single locus phenotype

FYPO:0003486 - abolished protein localization to double-strand break site

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Genotypes:

FYPO:0003484 - abolished protein localization to mitotic spindle midzone

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Genotypes:

FYPO:0000705 - abolished protein-protein interaction

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Genotypes:

FYPO:0002825 - decreased protein localization to mitotic spindle

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Genotypes:

FYPO:0001645 - decreased protein-protein interaction

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Genotypes:

FYPO:0003129 - loss of punctate nuclear protein localization during cellular response to ionizing radiation

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Genotypes:

FYPO:0002169 - normal growth during cellular response to gamma radiation

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Genotypes:

FYPO:0004229 - normal growth during cellular response to ionizing radiation

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Genotypes:

FYPO:0000969 - normal growth during cellular response to UV

References:

Genotypes:

FYPO:0001690 - normal growth on camptothecin

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Genotypes:

FYPO:0001164 - normal growth on glucose carbon source

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Genotypes:

FYPO:0000963 - normal growth on hydroxyurea

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Genotypes:

FYPO:0009038 - resistance to egtazic acid

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Genotypes:

FYPO:0009043 - resistance to potassium chloride and sodium dodecyl sulfate

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Genotypes:

FYPO:0000077 - resistance to rapamycin

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Genotypes:

FYPO:0000069 - resistance to thiabendazole

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Genotypes:

FYPO:0000095 - sensitive to bleomycin

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Genotypes:

FYPO:0001501 - sensitive to brefeldin A

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Genotypes:

FYPO:0000085 - sensitive to camptothecin

References:

Genotypes:

FYPO:0000799 - sensitive to diamide

References:

Genotypes:

FYPO:0000088 - sensitive to hydroxyurea

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Genotypes:

FYPO:0000267 - sensitive to ionizing radiation during vegetative growth

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Genotypes:

FYPO:0000089 - sensitive to methyl methanesulfonate

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Genotypes:

FYPO:0002550 - sensitive to UV

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Genotypes:

FYPO:0001491 - viable vegetative cell

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Genotypes:

FYPO:0002060 - viable vegetative cell population

References:

Genotypes:

FYPO:0002177 - viable vegetative cell with normal cell morphology

References:

Genotypes:

Taxonomic conservation

PBO:0011065 - conserved in eukaryotes

PBO:0011071 - conserved in eukaryotes only

PBO:0011064 - conserved in fungi

PBO:0011069 - conserved in metazoa

PBO:0011070 - conserved in vertebrates

PBO:0000055 - no apparent S. cerevisiae ortholog

PBO:0006222 - predominantly single copy (one to one)

Warnings

PBO:0000070 - gene structure updated

References:

Protein features

IDNameInterPro nameDB name
PF00533BRCTBRCT_domPfam
cd17744BRCT_MDC1_rpt1CDD
PS50172BRCTBRCT_domPROSITE profiles
SM00292BRCTBRCT_domSMART
G3DSA:3.40.50.10190:FF:000195CATH-FunFam
G3DSA:3.40.50.10190BRCT_dom_sfCATH-Gene3D
SSF52113BRCT_dom_sfSUPERFAMILY
PTHR23196DDR_Transcriptional_RegPANTHER
mobidb-lite-Disorderdisorder_predictionMobiDB-Disorder
mobidb-lite-Low-complexitydisorder_predictionMobiDB-Low-complexity
mobidb-lite-Polardisorder_predictionMobiDB-Polar
mobidb-lite-Polyampholytedisorder_predictionMobiDB-Polyampholyte

Orthologs

References / Literature

PMID:21712547 - Mitotic substrates of the kinase aurora with roles in chromatin regulation identified through quantitative phosphoproteomics of fission yeast.
Koch A et al. Sci Signal 2011 Jun 28;4(179):rs6
PMID:22907753 - Posttranscriptional regulation of cell-cell interaction protein-encoding transcripts by Zfs1p in Schizosaccharomyces pombe.
Wells ML et al. Mol Cell Biol 2012 Oct;32(20):4206-14
PMID:24806815 - Mdb1, a fission yeast homolog of human MDC1, modulates DNA damage response and mitotic spindle function.
Wei Y et al. PLoS One 2014;9(5):e97028
PMID:16823372 - ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe.
Matsuyama A et al. Nat Biotechnol 2006 Jul;24(7):841-7
PMID:19547744 - Evolution of phosphoregulation: comparison of phosphorylation patterns across yeast species.
Beltrao P et al. PLoS Biol 2009 Jun 16;7(6):e1000134
PMID:21511999 - Comparative functional genomics of the fission yeasts.
Rhind N et al. Science 2011 May 20;332(6032):930-6
PMID:24763107 - Absolute proteome and phosphoproteome dynamics during the cell cycle of Schizosaccharomyces pombe (Fission Yeast).
Carpy A et al. Mol Cell Proteomics 2014 Aug;13(8):1925-36
PMID:21270388 - Augmented annotation of the Schizosaccharomyces pombe genome reveals additional genes required for growth and viability.
Bitton DA et al. Genetics 2011 Apr;187(4):1207-17
PMID:37787768 - Broad functional profiling of fission yeast proteins using phenomics and machine learning.
Rodríguez-López M et al. Elife 2023 Oct 03;12
PMID:30295604 - BRCT domains of the DNA damage checkpoint proteins TOPBP1/Rad4 display distinct specificities for phosphopeptide ligands.
Day M et al. Elife 2018 Oct 08;7
PMID:29996109 - Quantitative Phosphoproteomics Reveals the Signaling Dynamics of Cell-Cycle Kinases in the Fission Yeast Schizosaccharomyces pombe.
Swaffer MP et al. Cell Rep 2018 Jul 10;24(2):503-514
PMID:37772819 - Activities, substrate specificity, and genetic interactions of fission yeast Siw14, a cysteinyl-phosphatase-type inositol pyrophosphatase.
Sanchez AM et al. mBio 2023 Sep 29;14(5):e0205623
PMID:26160178 - Dimerization Mediated by a Divergent Forkhead-associated Domain Is Essential for the DNA Damage and Spindle Functions of Fission Yeast Mdb1.
Luo S et al. J Biol Chem 2015 Aug 21;290(34):21054-21066
PMID:27298342 - Identification of S-phase DNA damage-response targets in fission yeast reveals conservation of damage-response networks.
Willis NA et al. Proc Natl Acad Sci U S A 2016 Jun 28;113(26):E3676-85
PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.
Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623
PMID:30726745 - Fission Yeast NDR/LATS Kinase Orb6 Regulates Exocytosis via Phosphorylation of the Exocyst Complex.
Tay YD et al. Cell Rep 2019 Feb 05;26(6):1654-1667.e7
GO_REF:0000024 - Manual transfer of experimentally-verified manual GO annotation data to orthologs by curator judgment of sequence similarity.
PMID:27168121 - Discovery of genes involved in mitosis, cell division, cell wall integrity and chromosome segregation through construction of Schizosaccharomyces pombe deletion strains.
Chen JS et al. Yeast 2016 Sep;33(9):507-17
PMID:26537787 - Targeting of SUMO substrates to a Cdc48-Ufd1-Npl4 segregase and STUbL pathway in fission yeast.
Køhler JB et al. Nat Commun 2015 Nov 05;6:8827
PMID:27984725 - CDK Substrate Phosphorylation and Ordering the Cell Cycle.
Swaffer MP et al. Cell 2016 Dec 15;167(7):1750-1761.e16
PMID:34678589 - Phosphorylation-dependent assembly of DNA damage response systems and the central roles of TOPBP1.
Day M et al. DNA Repair (Amst) 2021 Dec;108:103232
PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.
Marguerat S et al. Cell 2012 Oct 26;151(3):671-83
PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.
Hayles J et al. Open Biol 2013 May 22;3(5):130053
PMID:25720772 - Quantitative phosphoproteomics reveals pathways for coordination of cell growth and division by the conserved fission yeast kinase pom1.
Kettenbach AN et al. Mol Cell Proteomics 2015 May;14(5):1275-87
PMID:33823663 - A TOR (target of rapamycin) and nutritional phosphoproteome of fission yeast reveals novel targets in networks conserved in humans.
Halova L et al. Open Biol 2021 Apr;11(4):200405
PMID:39367033 - Quantitative proteomics and phosphoproteomics profiling of meiotic divisions in the fission yeast Schizosaccharomyces pombe.
Sivakova B et al. Sci Rep 2024 Oct 04;14(1):23105
PMID:39705284 - Proteomic and phosphoproteomic analyses reveal that TORC1 is reactivated by pheromone signaling during sexual reproduction in fission yeast.
Bérard M et al. PLoS Biol 2024 Dec 20;22(12):e3002963