protein coding gene - uaf2 (SPAP8A3.06) - U2 small nuclear RNA auxiliary factor small subunit, U2AF-23

Gene summary

Standard name

uaf2

Systematic ID

SPAP8A3.06

Product

U2 small nuclear RNA auxiliary factor small subunit, U2AF-23

Organism

Schizosaccharomyces pombe (fission yeast)

Synonyms

ods2

UniProt ID

Q09176

Characterisation status

biological role published

Feature type

mRNA gene

Genomic location

chromosome I: 5325594..5326435 forward strand

Annotation

Protein features

Orthologs

• U2AF1 (HGNC:12453) Homo sapiens
• U2AF1L4 (HGNC:23020) Homo sapiens
• uaf2 (SJAG_00225) Schizosaccharomyces japonicus

References / Literature

PMID:39367033 - Quantitative proteomics and phosphoproteomics profiling of meiotic divisions in the fission yeast Schizosaccharomyces pombe.

Sivakova B et al. Sci Rep 2024 Oct 04;14(1):23105

PMID:26771498 - A Proteome-wide Fission Yeast Interactome Reveals Network Evolution Principles from Yeasts to Human.

Vo TV et al. Cell 2016 Jan 14;164(1-2):310-323

PMID:17130122 - Mutations in the SF1-U2AF59-U2AF23 complex cause exon skipping in Schizosaccharomyces pombe.

Haraguchi N et al. J Biol Chem 2007 Jan 26;282(4):2221-8

PMID:32101745 - Dense Transposon Integration Reveals Essential Cleavage and Polyadenylation Factors Promote Heterochromatin Formation.

Lee SY et al. Cell Rep 2020 Feb 25;30(8):2686-2698.e8

PMID:23695164 - Cross-species protein interactome mapping reveals species-specific wiring of stress response pathways.

Das J et al. Sci Signal 2013 May 21;6(276):ra38

PMID:24763107 - Absolute proteome and phosphoproteome dynamics during the cell cycle of Schizosaccharomyces pombe (Fission Yeast).

Carpy A et al. Mol Cell Proteomics 2014 Aug;13(8):1925-36

PMID:12374752 - Pre-spliceosome formation in S.pombe requires a stable complex of SF1-U2AF(59)-U2AF(23).

Huang T et al. EMBO J 2002 Oct 15;21(20):5516-26

PMID:16823372 - ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe.

Matsuyama A et al. Nat Biotechnol 2006 Jul;24(7):841-7

PMID:26302002 - Cwf16p Associating with the Nineteen Complex Ensures Ordered Exon Joining in Constitutive Pre-mRNA Splicing in Fission Yeast.

Sasaki-Haraguchi N et al. PLoS One 2015;10(8):e0136336

PMID:39705284 - Proteomic and phosphoproteomic analyses reveal that TORC1 is reactivated by pheromone signaling during sexual reproduction in fission yeast.

Bérard M et al. PLoS Biol 2024 Dec 20;22(12):e3002963

PMID:9371883 - Molecular characterization of a novel fission yeast gene spUAP2 that interacts with the splicing factor spU2AF59.

McKinney R et al. Curr Genet 1997 Nov;32(5):323-30

PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.

Hayles J et al. Open Biol 2013 May 22;3(5):130053

PMID:40015273 - A comprehensive Schizosaccharomyces pombe atlas of physical transcription factor interactions with proteins and chromatin.

Skribbe M et al. Mol Cell 2025 Feb 19;

PMID:37970674 - SUMOylation regulates Lem2 function in centromere clustering and silencing.

Strachan J et al. J Cell Sci 2023 Dec 01;136(23)

PMID:32958768 - Elucidation of the aberrant 3' splice site selection by cancer-associated mutations on the U2AF1.

Yoshida H et al. Nat Commun 2020 Sep 21;11(1):4744

PMID:21511999 - Comparative functional genomics of the fission yeasts.

Rhind N et al. Science 2011 May 20;332(6032):930-6

PMID:9671485 - A potential role for U2AF-SAP 155 interactions in recruiting U2 snRNP to the branch site.

Gozani O et al. Mol Cell Biol 1998 Aug;18(8):4752-60

PMID:25720772 - Quantitative phosphoproteomics reveals pathways for coordination of cell growth and division by the conserved fission yeast kinase pom1.

Kettenbach AN et al. Mol Cell Proteomics 2015 May;14(5):1275-87

PMID:30726745 - Fission Yeast NDR/LATS Kinase Orb6 Regulates Exocytosis via Phosphorylation of the Exocyst Complex.

Tay YD et al. Cell Rep 2019 Feb 05;26(6):1654-1667.e7

PMID:15548596 - Analysis of mutant phenotypes and splicing defects demonstrates functional collaboration between the large and small subunits of the essential splicing factor U2AF in vivo.

Webb CJ et al. Mol Biol Cell 2005 Feb;16(2):584-96

PMID:30759079 - A systematic genetic screen identifies essential factors involved in nuclear size control.

Cantwell H et al. PLoS Genet 2019 Feb;15(2):e1007929

PMID:8657565 - The small subunit of the splicing factor U2AF is conserved in fission yeast.

Wentz-Hunter K et al. Nucleic Acids Res 1996 May 15;24(10):1849-54

PMID:24223771 - New insights into the RNA-based mechanism of action of the anticancer drug 5'-fluorouracil in eukaryotic cells.

Mojardín L et al. PLoS One 2013;8(11):e78172

PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.

Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623

PMID:15121844 - The splicing factor U2AF small subunit is functionally conserved between fission yeast and humans.

Webb CJ et al. Mol Cell Biol 2004 May;24(10):4229-40

PMID:28446597 - The histone variant H2A.Z promotes splicing of weak introns.

Nissen KE et al. Genes Dev 2017 Apr 01;31(7):688-701

PMID:24713849 - Post-transcriptional regulation of meiotic genes by a nuclear RNA silencing complex.

Egan ED et al. RNA 2014 Jun;20(6):867-81

PMID:24013502 - Epe1 recruits BET family bromodomain protein Bdf2 to establish heterochromatin boundaries.

Wang J et al. Genes Dev 2013 Sep 01;27(17):1886-902

PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.

Marguerat S et al. Cell 2012 Oct 26;151(3):671-83

PMID:26215567 - A novel 3' splice site recognition by the two zinc fingers in the U2AF small subunit.

Yoshida H et al. Genes Dev 2015 Aug 01;29(15):1649-60

PMID:22681890 - Hierarchical modularity and the evolution of genetic interactomes across species.

Ryan CJ et al. Mol Cell 2012 Jun 08;46(5):691-704

PMID:11414703 - The fission yeast ortholog of the coregulator SKIP interacts with the small subunit of U2AF.

Ambrozková M et al. Biochem Biophys Res Commun 2001 Jun 29;284(5):1148-54

PMID:33823663 - A TOR (target of rapamycin) and nutritional phosphoproteome of fission yeast reveals novel targets in networks conserved in humans.

Halova L et al. Open Biol 2021 Apr;11(4):200405

GO_REF:0000002 - Comments

PMID:28947618 - Sde2 is an intron-specific pre-mRNA splicing regulator activated by ubiquitin-like processing.

Thakran P et al. EMBO J 2018 Jan 04;37(1):89-101

PMID:10923022 - Mutations in the large subunit of U2AF disrupt pre-mRNA splicing, cell cycle progression and nuclear structure.

Beales M et al. Yeast 2000 Aug;16(11):1001-13

PMID:11686295 - Conserved Wat1/Pop3 WD-repeat protein of fission yeast secures genome stability through microtubule integrity and may be involved in mRNA maturation.

Ochotorena IL et al. J Cell Sci 2001 Aug;114(Pt 16):2911-20