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protein coding gene - glt1 (SPAPB1E7.07) - glutamate synthase (GOGAT) Glt1

Gene summary

Standard name
glt1
Systematic ID
SPAPB1E7.07
Product
glutamate synthase (GOGAT) Glt1
Organism
Schizosaccharomyces pombe (fission yeast)
Synonyms
gog1, glu4
UniProt ID
Q9C102
Characterisation status
biological role published
Feature type
mRNA gene
Genomic location
chromosome I: 3302825..3310369 forward strand

Annotation

Catalytic activity attributes

PBO:0091465 - L-glutamine substrate KM 800 uM, 2-oxoglutarate substrate KM 9 uM, NADH substrate KM 11 uM, pH optimum 6.4

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GO biological process

GO:0019676 - ammonia assimilation cycle

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GO:0097054 - L-glutamate biosynthetic process

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GO cellular component

GO:0005737 - cytoplasm

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GO:0005829 - cytosol

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GO:0005759 - mitochondrial matrix

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GO molecular function

GO:0050660 - flavin adenine dinucleotide binding

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GO:0010181 - FMN binding

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GO:0016040 - glutamate synthase (NADH) activity

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GO:0005506 - iron ion binding

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GO:0051536 - iron-sulfur cluster binding

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GO:0016639 - oxidoreductase activity, acting on the CH-NH2 group of donors, NAD or NADP as acceptor

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Modification

MOD:00046 - O-phospho-L-serine

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MOD:00047 - O-phospho-L-threonine

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MOD:01149 - sumoylated lysine

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MOD:01148 - ubiquitinylated lysine

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Qualitative gene expression

PomGeneEx:0000019 - protein level decreased

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PomGeneEx:0000012 - RNA level decreased

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Quantitative gene expression

PBO:0006310 - protein level

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PBO:0011963 - RNA level

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Single locus phenotype

FYPO:0005471 - abolished glutamate synthase (NADH) activity

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Genotypes:

FYPO:0000249 - decreased cell population growth on ammonia nitrogen source

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Genotypes:

FYPO:0008020 - decreased cell population growth on leucine nitrogen source

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Genotypes:

FYPO:0005877 - growth auxotrophic for glutamate

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Genotypes:

FYPO:0003938 - increased cell population growth during glucose starvation

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Genotypes:

FYPO:0009077 - increased cell population growth on ethanol carbon source

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Genotypes:

FYPO:0009094 - increased cell population growth on lysine and proline nitrogen source

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Genotypes:

FYPO:0009098 - increased cell population growth on mannitol carbon source

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Genotypes:

FYPO:0009096 - increased cell population growth on xylose carbon source

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Genotypes:

FYPO:0008018 - normal growth on glutamine nitrogen source

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Genotypes:

FYPO:0000067 - resistance to brefeldin A

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Genotypes:

FYPO:0000763 - resistance to cadmium

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Genotypes:

FYPO:0009079 - resistance to calcofluor and sodium dodecyl sulfate

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Genotypes:

FYPO:0000764 - resistance to cycloheximide

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Genotypes:

FYPO:0002693 - resistance to diamide

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Genotypes:

FYPO:0002578 - resistance to hydroxyurea

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Genotypes:

FYPO:0009043 - resistance to potassium chloride and sodium dodecyl sulfate

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Genotypes:

FYPO:0001034 - resistance to tunicamycin

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Genotypes:

FYPO:0000842 - sensitive to ethanol during vegetative growth

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Genotypes:

FYPO:0001719 - sensitive to lithium

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Genotypes:

FYPO:0009086 - sensitive to lithium chloride and sodium dodecyl sulfate

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Genotypes:

FYPO:0009088 - sensitive to magnesium chloride and sodium dodecyl sulfate

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Genotypes:

FYPO:0002060 - viable vegetative cell population

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Genotypes:

FYPO:0002177 - viable vegetative cell with normal cell morphology

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Genotypes:

Taxonomic conservation

PBO:0011067 - conserved in bacteria

PBO:0011065 - conserved in eukaryotes

PBO:0011064 - conserved in fungi

PBO:0011069 - conserved in metazoa

PBO:0006222 - predominantly single copy (one to one)

Protein features

IDNameInterPro nameDB name
PF00310GATase_2GATase_2_domPfam
PF01493GXGXGGlu_synth_asu_CPfam
PF01645Glu_synthaseGlu_synthdomPfam
PF04898Glu_syn_centralGlu_synth_centr_NPfam
PF07992Pyr_redox_2FAD/NAD-binding_domPfam
PF14691Fer4_20DPD_IIPfam
cd00713GltSCDD
cd00982gltB_CGlu_synth_asu_CCDD
cd02808GltS_FMNGlu_synthdomCDD
PS51278GATASE_TYPE_2GATase_2_domPROSITE profiles
PR00419ADXRDTASEPRINTS
G3DSA:2.160.20.60:FF:000001CATH-FunFam
G3DSA:3.20.20.70:FF:000017CATH-FunFam
G3DSA:3.20.20.70:FF:000031CATH-FunFam
G3DSA:3.40.50.720:FF:000113CATH-FunFam
G3DSA:3.50.50.60:FF:000022CATH-FunFam
G3DSA:3.60.20.10:FF:000001CATH-FunFam
G3DSA:1.10.1060.10Helical_ferredxnCATH-Gene3D
G3DSA:2.160.20.60Glu_synth_asu_C_sfCATH-Gene3D
G3DSA:3.20.20.70Aldolase_TIMCATH-Gene3D
G3DSA:3.40.50.720CATH-Gene3D
G3DSA:3.50.50.60FAD/NAD-bd_sfCATH-Gene3D
G3DSA:3.60.20.10Ntn_hydrolases_NCATH-Gene3D
SSF46548SUPERFAMILY
SSF51395SUPERFAMILY
SSF51971SUPERFAMILY
SSF56235Ntn_hydrolases_NSUPERFAMILY
SSF69336Glu_synth_asu_C_sfSUPERFAMILY
PTHR43100Glutamate_SynthasePANTHER
PIRSF000187GOGATGlu_synth_eukPIRSF
NF008730gltBNCBIFAM
TIGR01317GOGAT_sm_gamGlut_synth_ssu1NCBIFAM
mobidb-lite-Disorderdisorder_predictionMobiDB-Disorder

Orthologs

References / Literature

PMID:7903653 - Enzyme defects in glutamate-requiring strains of Schizosaccharomyces pombe.
Barel I et al. FEMS Microbiol Lett 1993 Nov 01;113(3):267-72
PMID:30726745 - Fission Yeast NDR/LATS Kinase Orb6 Regulates Exocytosis via Phosphorylation of the Exocyst Complex.
Tay YD et al. Cell Rep 2019 Feb 05;26(6):1654-1667.e7
PMID:37787768 - Broad functional profiling of fission yeast proteins using phenomics and machine learning.
Rodríguez-López M et al. Elife 2023 Oct 03;12
PMID:25720772 - Quantitative phosphoproteomics reveals pathways for coordination of cell growth and division by the conserved fission yeast kinase pom1.
Kettenbach AN et al. Mol Cell Proteomics 2015 May;14(5):1275-87
PMID:26412298 - A Degenerate Cohort of Yeast Membrane Trafficking DUBs Mediates Cell Polarity and Survival.
Beckley JR et al. Mol Cell Proteomics 2015 Dec;14(12):3132-41
PMID:26537787 - Targeting of SUMO substrates to a Cdc48-Ufd1-Npl4 segregase and STUbL pathway in fission yeast.
Køhler JB et al. Nat Commun 2015 Nov 05;6:8827
PMID:7773104 - Glutamine synthetase/glutamate synthase ammonium-assimilating pathway in Schizosaccharomyces pombe.
Perysinakis A et al. Curr Microbiol 1995 Jun;30(6):367-72
PMID:31064814 - Proximity-dependent biotinylation mediated by TurboID to identify protein-protein interaction networks in yeast.
Larochelle M et al. J Cell Sci 2019 May 31;132(11)
PMID:28218250 - Chromatin remodeller Fun30 Fft3 induces nucleosome disassembly to facilitate RNA polymerase II elongation.
Lee J et al. Nat Commun 2017 Feb 20;8:14527
PMID:20435771 - Iron-dependent remodeling of fungal metabolic pathways associated with ferrichrome biosynthesis.
Mercier A et al. Appl Environ Microbiol 2010 Jun;76(12):3806-17
GO_REF:0000002 - Comments
PMID:28982178 - Filamentous invasive growth of mutants of the genes encoding ammonia-metabolizing enzymes in the fission yeast Schizosaccharomyces pombe.
Sasaki Y et al. PLoS One 2017;12(10):e0186028
PMID:26152728 - A Two-step Protein Quality Control Pathway for a Misfolded DJ-1 Variant in Fission Yeast.
Mathiassen SG et al. J Biol Chem 2015 Aug 21;290(34):21141-21153
PMID:30355493 - Expanded Interactome of the Intrinsically Disordered Protein Dss1.
Schenstrøm SM et al. Cell Rep 2018 Oct 23;25(4):862-870
PMID:21511999 - Comparative functional genomics of the fission yeasts.
Rhind N et al. Science 2011 May 20;332(6032):930-6
PMID:33823663 - A TOR (target of rapamycin) and nutritional phosphoproteome of fission yeast reveals novel targets in networks conserved in humans.
Halova L et al. Open Biol 2021 Apr;11(4):200405
PMID:16823372 - ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe.
Matsuyama A et al. Nat Biotechnol 2006 Jul;24(7):841-7
PMID:39705284 - Proteomic and phosphoproteomic analyses reveal that TORC1 is reactivated by pheromone signaling during sexual reproduction in fission yeast.
Bérard M et al. PLoS Biol 2024 Dec 20;22(12):e3002963
PMID:18223116 - Key function for the CCAAT-binding factor Php4 to regulate gene expression in response to iron deficiency in fission yeast.
Mercier A et al. Eukaryot Cell 2008 Mar;7(3):493-508
PMID:39367033 - Quantitative proteomics and phosphoproteomics profiling of meiotic divisions in the fission yeast Schizosaccharomyces pombe.
Sivakova B et al. Sci Rep 2024 Oct 04;14(1):23105
GO_REF:0000111 - Gene Ontology annotations Inferred by Curator (IC) using at least one Inferred by Sequence Similarity (ISS) annotation to support the inference
PMID:23050226 - A genetic screen to discover pathways affecting cohesin function in Schizosaccharomyces pombe identifies chromatin effectors.
Chen Z et al. G3 (Bethesda) 2012 Oct;2(10):1161-8
PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.
Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623
PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.
Marguerat S et al. Cell 2012 Oct 26;151(3):671-83
PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.
Hayles J et al. Open Biol 2013 May 22;3(5):130053
PMID:37970674 - SUMOylation regulates Lem2 function in centromere clustering and silencing.
Strachan J et al. J Cell Sci 2023 Dec 01;136(23)
PMID:40015273 - A comprehensive Schizosaccharomyces pombe atlas of physical transcription factor interactions with proteins and chromatin.
Skribbe M et al. Mol Cell 2025 Feb 19;
PMID:4148397 - Presence of glutamate synthase in fission yeasts and its possible role in ammonia assimilation.
Brown CM et al. Nat New Biol 1973 Nov 28;246(152):115-6
PMID:24763107 - Absolute proteome and phosphoproteome dynamics during the cell cycle of Schizosaccharomyces pombe (Fission Yeast).
Carpy A et al. Mol Cell Proteomics 2014 Aug;13(8):1925-36