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protein coding gene - prp12 (SPAPJ698.03c) - U2 snRNP-associated protein, WD repeat protein Sap130

Gene summary

Standard name
prp12
Systematic ID
SPAPJ698.03c
Product
U2 snRNP-associated protein, WD repeat protein Sap130
Organism
Schizosaccharomyces pombe (fission yeast)
Synonyms
sap130, SF3b130
UniProt ID
Q9UTT2
Characterisation status
biological role published
Feature type
mRNA gene
Genomic location
chromosome I: 4037950..4041796 reverse strand

Annotation

GO biological process

GO:0045292 - mRNA cis splicing, via spliceosome

References:

GO:0000245 - spliceosomal complex assembly

References:

GO cellular component

GO:0005634 - nucleus

References:

GO:0005686 - U2 snRNP

References:

GO:0071004 - U2-type prespliceosome

References:

GO molecular function

GO:0005515 - protein binding

References:

GO:0030620 - U2 snRNA binding

References:

Modification

MOD:01148 - ubiquitinylated lysine

References:

Multi-locus phenotype

FYPO:0001235 - decreased extent of cell population growth

References:

Genotypes:

FYPO:0001355 - decreased vegetative cell population growth

References:

Genotypes:

FYPO:0002061 - inviable vegetative cell population

References:

Genotypes:

Qualitative gene expression

PomGeneEx:0000012 - RNA level decreased

References:

Quantitative gene expression

PBO:0006310 - protein level

References:

PBO:0011963 - RNA level

References:

Single locus phenotype

FYPO:0000444 - abnormal mitotic cell cycle arrest with replicated DNA

References:

Genotypes:

FYPO:0000082 - decreased cell population growth at high temperature

References:

Genotypes:

FYPO:0004201 - decreased centromeric outer repeat transcript-derived siRNA level

References:

Genotypes:

FYPO:0003412 - decreased chromatin silencing at centromere outer repeat

References:

Genotypes:

FYPO:0003029 - decreased mRNA splicing, via spliceosome

References:

Genotypes:

FYPO:0003041 - decreased snRNA splicing

References:

Genotypes:

FYPO:0000220 - increased centromeric outer repeat transcript level

References:

Genotypes:

FYPO:0001908 - increased pre-mRNA level

References:

Genotypes:

FYPO:0000839 - inviable elongated mononucleate aseptate cell

References:

Genotypes:

FYPO:0001490 - inviable elongated vegetative cell

References:

Genotypes:

FYPO:0002061 - inviable vegetative cell population

References:

Genotypes:

FYPO:0001511 - inviable vegetative cell, abnormal cell shape, normal cell size

References:

Genotypes:

FYPO:0003619 - normal mRNA splicing, via spliceosome

References:

Genotypes:

FYPO:0003620 - normal pre-mRNA level

References:

Genotypes:

FYPO:0000785 - sensitive to formamide

References:

Genotypes:

Taxonomic conservation

PBO:0011065 - conserved in eukaryotes

PBO:0011071 - conserved in eukaryotes only

PBO:0011064 - conserved in fungi

PBO:0011069 - conserved in metazoa

PBO:0011070 - conserved in vertebrates

PBO:0006222 - predominantly single copy (one to one)

Protein features

IDNameInterPro nameDB name
PF03178CPSF_ARSE1/DDB1/CPSF1_CPfam
PF10433Beta-prop_RSE1_1stBeta-prop_RSE1/DDB1/CPSF1_1stPfam
PF23726Beta-prop_RSE1_2ndBeta-prop_RSE1/DDB1/CPSF1_2ndPfam
G3DSA:2.130.10.10:FF:000031CATH-FunFam
G3DSA:2.130.10.10:FF:001143CATH-FunFam
G3DSA:2.130.10.10WD40/YVTN_repeat-like_dom_sfCATH-Gene3D
SSF50978WD40_repeat_dom_sfSUPERFAMILY
PTHR10644RSE1/DDB1/CFT1PANTHER
CoilCOILS

Orthologs

References / Literature

GO_REF:0000024 - Manual transfer of experimentally-verified manual GO annotation data to orthologs by curator judgment of sequence similarity.
PMID:29162938 - RNA metabolism is the primary target of formamide in vivo.
Hoyos-Manchado R et al. Sci Rep 2017 Nov 21;7(1):15895
PMID:24081329 - Poly(A) tail-mediated gene regulation by opposing roles of Nab2 and Pab2 nuclear poly(A)-binding proteins in pre-mRNA decay.
Grenier St-Sauveur V et al. Mol Cell Biol 2013 Dec;33(23):4718-31
PMID:15755920 - Dim1p is required for efficient splicing and export of mRNA encoding lid1p, a component of the fission yeast anaphase-promoting complex.
Carnahan RH et al. Eukaryot Cell 2005 Mar;4(3):577-87
PMID:22064476 - A U1-U2 snRNP interaction network during intron definition.
Shao W et al. Mol Cell Biol 2012 Jan;32(2):470-8
PMID:24013502 - Epe1 recruits BET family bromodomain protein Bdf2 to establish heterochromatin boundaries.
Wang J et al. Genes Dev 2013 Sep 01;27(17):1886-902
PMID:25274039 - A systematic genetic screen identifies new factors influencing centromeric heterochromatin integrity in fission yeast.
Bayne EH et al. Genome Biol 2014;15(10):481
PMID:24014766 - Structural and functional characterization of the N terminus of Schizosaccharomyces pombe Cwf10.
Livesay SB et al. Eukaryot Cell 2013 Nov;12(11):1472-89
PMID:37970674 - SUMOylation regulates Lem2 function in centromere clustering and silencing.
Strachan J et al. J Cell Sci 2023 Dec 01;136(23)
PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.
Marguerat S et al. Cell 2012 Oct 26;151(3):671-83
PMID:24713849 - Post-transcriptional regulation of meiotic genes by a nuclear RNA silencing complex.
Egan ED et al. RNA 2014 Jun;20(6):867-81
PMID:9003295 - Isolation of novel pre-mRNA splicing mutants of Schizosaccharomyces pombe.
Urushiyama S et al. Mol Gen Genet 1996 Nov 27;253(1-2):118-27
PMID:18948543 - Splicing factors facilitate RNAi-directed silencing in fission yeast.
Bayne EH et al. Science 2008 Oct 24;322(5901):602-6
PMID:24442611 - Endogenous U2·U5·U6 snRNA complexes in S. pombe are intron lariat spliceosomes.
Chen W et al. RNA 2014 Mar;20(3):308-20
PMID:22681890 - Hierarchical modularity and the evolution of genetic interactomes across species.
Ryan CJ et al. Mol Cell 2012 Jun 08;46(5):691-704
PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.
Hayles J et al. Open Biol 2013 May 22;3(5):130053
PMID:20018856 - Involvement of the spliceosomal U4 small nuclear RNA in heterochromatic gene silencing at fission yeast centromeres.
Chinen M et al. J Biol Chem 2010 Feb 19;285(8):5630-8
PMID:21386897 - Systematic two-hybrid and comparative proteomic analyses reveal novel yeast pre-mRNA splicing factors connected to Prp19.
Ren L et al. PLoS One 2011 Feb 28;6(2):e16719
GO_REF:0000033 - Annotation inferences using phylogenetic trees
PMID:24763107 - Absolute proteome and phosphoproteome dynamics during the cell cycle of Schizosaccharomyces pombe (Fission Yeast).
Carpy A et al. Mol Cell Proteomics 2014 Aug;13(8):1925-36
PMID:16537923 - Sterol regulatory element binding protein is a principal regulator of anaerobic gene expression in fission yeast.
Todd BL et al. Mol Cell Biol 2006 Apr;26(7):2817-31
PMID:11350031 - Mutation in the prp12+ gene encoding a homolog of SAP130/SF3b130 causes differential inhibition of pre-mRNA splicing and arrest of cell-cycle progression in Schizosaccharomyces pombe.
Habara Y et al. RNA 2001 May;7(5):671-81
PMID:21511999 - Comparative functional genomics of the fission yeasts.
Rhind N et al. Science 2011 May 20;332(6032):930-6
PMID:28446597 - The histone variant H2A.Z promotes splicing of weak introns.
Nissen KE et al. Genes Dev 2017 Apr 01;31(7):688-701
PMID:16049013 - Regulation of Cdc2p and Cdc13p is required for cell cycle arrest induced by defective RNA splicing in fission yeast.
Shimada M et al. J Biol Chem 2005 Sep 23;280(38):32640-8
PMID:28947618 - Sde2 is an intron-specific pre-mRNA splicing regulator activated by ubiquitin-like processing.
Thakran P et al. EMBO J 2018 Jan 04;37(1):89-101
PMID:37637271 - Isolation of mutant alleles of the U6 snRNA m 6 A methyltransferase Mtl16 and characterization of their genetic interactions with splicing mutants in Schizosaccharomyces pombe .
Willet AH et al. MicroPubl Biol 2023;2023
PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.
Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623