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protein coding gene - mid2 (SPAPYUG7.03c) - medial ring protein, anillin Mid2

Gene summary

Standard name
mid2
Systematic ID
SPAPYUG7.03c
Product
medial ring protein, anillin Mid2
Organism
Schizosaccharomyces pombe (fission yeast)
UniProt ID
Q9P7Y8
Characterisation status
biological role published
Feature type
mRNA gene
Genomic location
chromosome I: 4748861..4751453 reverse strand

Annotation

Disease association

MONDO:0014462 - focal segmental glomerulosclerosis 8

References:

GO biological process

GO:0031106 - septin ring organization

References:

GO cellular component

GO:0032153 - cell division site

References:

GO:0005737 - cytoplasm

References:

GO:0005829 - cytosol

References:

GO:0031097 - medial cortex

References:

GO:0036391 - medial cortex septin ring

References:

GO:0032176 - split septin rings

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GO molecular function

GO:0005525 - GTP binding

References:

GO:0005546 - phosphatidylinositol-4,5-bisphosphate binding

References:

Modification

MOD:00046 - O-phospho-L-serine

References:

MOD:00696 - phosphorylated residue

References:

MOD:01149 - sumoylated lysine

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MOD:01148 - ubiquitinylated lysine

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Multi-locus phenotype

FYPO:0001971 - abnormal cell separation after cytokinesis resulting in chained cells

References:

Genotypes:

FYPO:0006399 - abnormal septin ring morphology

References:

Genotypes:

FYPO:0006060 - abolished protein localization to septin ring

References:

Genotypes:

FYPO:0001223 - binucleate multiseptate vegetative cell

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Genotypes:

FYPO:0000134 - branched, elongated, multiseptate cell

References:

Genotypes:

FYPO:0003440 - cell lysis during cytokinesis

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Genotypes:

FYPO:0000223 - elongated multiseptate vegetative cell

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Genotypes:

FYPO:0000650 - increased septation index

References:

Genotypes:

FYPO:0000339 - mislocalized septum during vegetative growth

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Genotypes:

FYPO:0003500 - viable branched, elongated, multiseptate vegetative cell

References:

Genotypes:

Protein features

PBO:0111759 - pleckstrin homology domain

Qualitative gene expression

PomGeneEx:0000012 - RNA level decreased

References:

PomGeneEx:0000011 - RNA level increased

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Quantitative gene expression

PBO:0006310 - protein level

References:

PBO:0011963 - RNA level

References:

Single locus phenotype

FYPO:0001971 - abnormal cell separation after cytokinesis resulting in chained cells

References:

Genotypes:

FYPO:0000059 - abnormal mitotic cell cycle

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Genotypes:

FYPO:0006399 - abnormal septin ring morphology

References:

Genotypes:

FYPO:0000132 - abnormal septum disassembly

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Genotypes:

FYPO:0001018 - abolished NETO

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Genotypes:

FYPO:0002555 - abolished protein localization to medial cortex during vegetative growth

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Genotypes:

FYPO:0007027 - abolished protein localization to medial cortex, with protein mislocalized to cytoplasm, during vegetative growth

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Genotypes:

FYPO:0006060 - abolished protein localization to septin ring

References:

Genotypes:

FYPO:0009073 - decreased cell population growth on lysine nitrogen source

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Genotypes:

FYPO:0002924 - decreased cell population growth on maltose carbon source

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Genotypes:

FYPO:0002797 - decreased protein degradation

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Genotypes:

FYPO:0001355 - decreased vegetative cell population growth

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Genotypes:

FYPO:0000223 - elongated multiseptate vegetative cell

References:

Genotypes:

FYPO:0005261 - increased cell population growth on galactose carbon source

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Genotypes:

FYPO:0009101 - increased cell population growth on glycerol and galactose carbon source

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Genotypes:

FYPO:0009094 - increased cell population growth on lysine and proline nitrogen source

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Genotypes:

FYPO:0009098 - increased cell population growth on mannitol carbon source

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Genotypes:

FYPO:0009028 - increased cell population growth on proline nitrogen source

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Genotypes:

FYPO:0009096 - increased cell population growth on xylose carbon source

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Genotypes:

FYPO:0000636 - increased cell population growth rate

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Genotypes:

FYPO:0000650 - increased septation index

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Genotypes:

FYPO:0004557 - increased vegetative cell population growth

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Genotypes:

FYPO:0000245 - loss of viability in stationary phase

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Genotypes:

FYPO:0006015 - multiseptate cell with shmoo

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Genotypes:

FYPO:0000118 - multiseptate vegetative cell

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Genotypes:

FYPO:0004097 - normal actomyosin contractile ring contraction

References:

Genotypes:

FYPO:0004652 - normal actomyosin contractile ring morphology

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Genotypes:

FYPO:0000674 - normal cell population growth at high temperature

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Genotypes:

FYPO:0002141 - normal cell population growth at low temperature

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Genotypes:

FYPO:0001022 - normal growth during cellular response to high osmolarity

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Genotypes:

FYPO:0001037 - normal growth during cellular response to salt stress

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Genotypes:

FYPO:0002442 - normal protein localization to cell division site

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Genotypes:

FYPO:0002558 - normal protein localization to medial cortex during vegetative growth

References:

Genotypes:

FYPO:0007028 - normal protein localization to medial cortex septin ring during vegetative growth

References:

Genotypes:

FYPO:0004895 - normal rate of actomyosin contractile ring contraction

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Genotypes:

FYPO:0001420 - normal vegetative cell population growth rate

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Genotypes:

FYPO:0009085 - resistance to lithium chloride and sodium dodecyl sulfate

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Genotypes:

FYPO:0000077 - resistance to rapamycin

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Genotypes:

FYPO:0000327 - resistance to trichostatin A

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Genotypes:

FYPO:0007933 - sensitive to 2,2′-dipyridyl

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Genotypes:

FYPO:0001097 - sensitive to amitrole

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Genotypes:

FYPO:0009067 - sensitive to amorolfine

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Genotypes:

FYPO:0000095 - sensitive to bleomycin

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Genotypes:

FYPO:0000097 - sensitive to caffeine during vegetative growth

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Genotypes:

FYPO:0000104 - sensitive to cycloheximide

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Genotypes:

FYPO:0000799 - sensitive to diamide

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Genotypes:

FYPO:0007931 - sensitive to egtazic acid

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Genotypes:

FYPO:0000842 - sensitive to ethanol during vegetative growth

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Genotypes:

FYPO:0007928 - sensitive to ethylenediaminetetraacetic acid

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Genotypes:

FYPO:0000785 - sensitive to formamide

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Genotypes:

FYPO:0000087 - sensitive to hydrogen peroxide

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Genotypes:

FYPO:0000088 - sensitive to hydroxyurea

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Genotypes:

FYPO:0001719 - sensitive to lithium

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Genotypes:

FYPO:0009086 - sensitive to lithium chloride and sodium dodecyl sulfate

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Genotypes:

FYPO:0009088 - sensitive to magnesium chloride and sodium dodecyl sulfate

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Genotypes:

FYPO:0000089 - sensitive to methyl methanesulfonate

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Genotypes:

FYPO:0001214 - sensitive to potassium chloride

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Genotypes:

FYPO:0007924 - sensitive to potassium chloride and sodium dodecyl sulfate

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Genotypes:

FYPO:0005889 - sensitive to sodium chloride

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Genotypes:

FYPO:0002328 - sensitive to terbinafine

References:

Genotypes:

FYPO:0000797 - sensitive to tert-butyl hydroperoxide

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Genotypes:

FYPO:0000115 - sensitive to valproic acid

References:

Genotypes:

FYPO:0003656 - sensitive to vanadate

References:

Genotypes:

FYPO:0006821 - slow vegetative cell growth

References:

Genotypes:

FYPO:0001234 - slow vegetative cell population growth

References:

Genotypes:

FYPO:0000021 - spheroid vegetative cell

References:

Genotypes:

FYPO:0002459 - viable branched, elongated vegetative cell

References:

Genotypes:

FYPO:0001492 - viable elongated vegetative cell

References:

Genotypes:

FYPO:0002060 - viable vegetative cell population

References:

Genotypes:

Taxonomic conservation

PBO:0011065 - conserved in eukaryotes

PBO:0011071 - conserved in eukaryotes only

PBO:0011064 - conserved in fungi

PBO:0011069 - conserved in metazoa

PBO:0011070 - conserved in vertebrates

PBO:0006222 - predominantly single copy (one to one)

Warnings

PBO:0000070 - gene structure updated

References:

Protein features

IDNameInterPro nameDB name
PF00169PHPH_domainPFAM
cd13278PH_Bud4CDD
PS50003PH_DOMAINPH_domainPROSITE_PROFILES
SM00233PH_updatePH_domainSMART
SSF50729PH domain-likeSUPERFAMILY
G3DSA:2.30.29.30PH-like_dom_sfGENE3D
PTHR36100BUD SITE SELECTION PROTEIN 4Bud4PANTHER
mobidb-lite-Disorderdisorder_predictionMOBIDB-Disorder
mobidb-lite-Polardisorder_predictionMOBIDB-Polar

Orthologs

References / Literature

PMID:33313903 - Ribosome profiling reveals ribosome stalling on tryptophan codons and ribosome queuing upon oxidative stress in fission yeast.
Rubio A et al. Nucleic Acids Res 2021 Jan 11;49(1):383-399
PMID:20739711 - Cooperation between the septins and the actomyosin ring and role of a cell-integrity pathway during cell division in fission yeast.
Wu JQ et al. Genetics 2010 Nov;186(3):897-915
PMID:16415366 - A role for the septation initiation network in septum assembly revealed by genetic analysis of sid2-250 suppressors.
Jin QW et al. Genetics 2006 Apr;172(4):2101-12
PMID:23093943 - Cytokinesis-based constraints on polarized cell growth in fission yeast.
Bohnert KA et al. PLoS Genet 2012;8(10):e1003004
PMID:24947517 - The putative exchange factor Gef3p interacts with Rho3p GTPase and the septin ring during cytokinesis in fission yeast.
Muñoz S et al. J Biol Chem 2014 Aug 08;289(32):21995-2007
PMID:16823372 - ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe.
Matsuyama A et al. Nat Biotechnol 2006 Jul;24(7):841-7
GO_REF:0000033 - Annotation inferences using phylogenetic trees
PMID:25720772 - Quantitative phosphoproteomics reveals pathways for coordination of cell growth and division by the conserved fission yeast kinase pom1.
Kettenbach AN et al. Mol Cell Proteomics 2015 May;14(5):1275-87
PMID:30726745 - Fission Yeast NDR/LATS Kinase Orb6 Regulates Exocytosis via Phosphorylation of the Exocyst Complex.
Tay YD et al. Cell Rep 2019 Feb 05;26(6):1654-1667.e7
PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.
Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623
PMID:16224022 - Counting cytokinesis proteins globally and locally in fission yeast.
Wu JQ et al. Science 2005 Oct 14;310(5746):310-4
PMID:15689498 - Ace2p controls the expression of genes required for cell separation in Schizosaccharomyces pombe.
Alonso-Nuñez ML et al. Mol Biol Cell 2005 Apr;16(4):2003-17
PMID:22681890 - Hierarchical modularity and the evolution of genetic interactomes across species.
Ryan CJ et al. Mol Cell 2012 Jun 08;46(5):691-704
PMID:29432178 - General amino acid control in fission yeast is regulated by a nonconserved transcription factor, with functions analogous to Gcn4/Atf4.
Duncan CDS et al. Proc Natl Acad Sci U S A 2018 Feb 20;115(8):E1829-E1838
PMID:36478272 - Translation-complex profiling of fission yeast cells reveals dynamic rearrangements of scanning ribosomal subunits upon nutritional stress.
Duncan CDS et al. Nucleic Acids Res 2022 Dec 09;50(22):13011-13025
PMID:37970674 - SUMOylation regulates Lem2 function in centromere clustering and silencing.
Strachan J et al. J Cell Sci 2023 Dec 01;136(23)
PMID:18257517 - Phosphoproteome analysis of fission yeast.
Wilson-Grady JT et al. J Proteome Res 2008 Mar;7(3):1088-97
PMID:24763107 - Absolute proteome and phosphoproteome dynamics during the cell cycle of Schizosaccharomyces pombe (Fission Yeast).
Carpy A et al. Mol Cell Proteomics 2014 Aug;13(8):1925-36
PMID:16317047 - Ace2p contributes to fission yeast septin ring assembly by regulating mid2+ expression.
Petit CS et al. J Cell Sci 2005 Dec 15;118(Pt 24):5731-42
PMID:27984744 - Survival in Quiescence Requires the Euchromatic Deployment of Clr4/SUV39H by Argonaute-Associated Small RNAs.
Joh RI et al. Mol Cell 2016 Dec 15;64(6):1088-1101
PMID:29996109 - Quantitative Phosphoproteomics Reveals the Signaling Dynamics of Cell-Cycle Kinases in the Fission Yeast Schizosaccharomyces pombe.
Swaffer MP et al. Cell Rep 2018 Jul 10;24(2):503-514
PMID:21712547 - Mitotic substrates of the kinase aurora with roles in chromatin regulation identified through quantitative phosphoproteomics of fission yeast.
Koch A et al. Sci Signal 2011 Jun 28;4(179):rs6
PMID:24972934 - Cross talk between NDR kinase pathways coordinates cytokinesis with cell separation in Schizosaccharomyces pombe.
Gupta S et al. Eukaryot Cell 2014 Aug;13(8):1104-12
PMID:12668659 - An anillin homologue, Mid2p, acts during fission yeast cytokinesis to organize the septin ring and promote cell separation.
Tasto JJ et al. J Cell Biol 2003 Mar 31;160(7):1093-103
PMID:37787768 - Broad functional profiling of fission yeast proteins using phenomics and machine learning.
Rodríguez-López M et al. Elife 2023 Oct 03;12
PMID:8946912 - The dmf1/mid1 gene is essential for correct positioning of the division septum in fission yeast.
Sohrmann M et al. Genes Dev 1996 Nov 01;10(21):2707-19
PMID:25076038 - Large scale screening of genetic interaction with sgf73(+) in fission yeast.
Guo Y et al. Yi Chuan 2014 Jul;36(7):723-31
PMID:32059768 - Size-Dependent Increase in RNA Polymerase II Initiation Rates Mediates Gene Expression Scaling with Cell Size.
Sun XM et al. Curr Biol 2020 Apr 06;30(7):1217-1230.e7
PMID:40015273 - A comprehensive Schizosaccharomyces pombe atlas of physical transcription factor interactions with proteins and chromatin.
Skribbe M et al. Mol Cell 2025 Feb 19;
PMID:26412298 - A Degenerate Cohort of Yeast Membrane Trafficking DUBs Mediates Cell Polarity and Survival.
Beckley JR et al. Mol Cell Proteomics 2015 Dec;14(12):3132-41
PB_REF:0000006 - Disease associations from Monarch via human-pombe orthologs
PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.
Hayles J et al. Open Biol 2013 May 22;3(5):130053
PMID:25015293 - Septin ring assembly is regulated by Spt20, a structural subunit of the SAGA complex.
Lei B et al. J Cell Sci 2014 Sep 15;127(Pt 18):4024-36
PMID:28410370 - A systematic screen for morphological abnormalities during fission yeast sexual reproduction identifies a mechanism of actin aster formation for cell fusion.
Dudin O et al. PLoS Genet 2017 Apr;13(4):e1006721
PMID:28784611 - Phosphoinositide-mediated ring anchoring resists perpendicular forces to promote medial cytokinesis.
Snider CE et al. J Cell Biol 2017 Oct 02;216(10):3041-3050
PMID:33823663 - A TOR (target of rapamycin) and nutritional phosphoproteome of fission yeast reveals novel targets in networks conserved in humans.
Halova L et al. Open Biol 2021 Apr;11(4):200405
PMID:39705284 - Proteomic and phosphoproteomic analyses reveal that TORC1 is reactivated by pheromone signaling during sexual reproduction in fission yeast.
Bérard M et al. PLoS Biol 2024 Dec 20;22(12):e3002963
PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.
Marguerat S et al. Cell 2012 Oct 26;151(3):671-83
PMID:25452419 - Parallel profiling of fission yeast deletion mutants for proliferation and for lifespan during long-term quiescence.
Sideri T et al. G3 (Bethesda) 2014 Dec 01;5(1):145-55
PMID:18256290 - Schizosaccharomyces pombe Pxl1 is a paxillin homologue that modulates Rho1 activity and participates in cytokinesis.
Pinar M et al. Mol Biol Cell 2008 Apr;19(4):1727-38
PMID:26537787 - Targeting of SUMO substrates to a Cdc48-Ufd1-Npl4 segregase and STUbL pathway in fission yeast.
Køhler JB et al. Nat Commun 2015 Nov 05;6:8827
PMID:21511999 - Comparative functional genomics of the fission yeasts.
Rhind N et al. Science 2011 May 20;332(6032):930-6
PMID:19547744 - Evolution of phosphoregulation: comparison of phosphorylation patterns across yeast species.
Beltrao P et al. PLoS Biol 2009 Jun 16;7(6):e1000134
PMID:12654901 - Mid2p stabilizes septin rings during cytokinesis in fission yeast.
Berlin A et al. J Cell Biol 2003 Mar 31;160(7):1083-92
PMID:27298342 - Identification of S-phase DNA damage-response targets in fission yeast reveals conservation of damage-response networks.
Willis NA et al. Proc Natl Acad Sci U S A 2016 Jun 28;113(26):E3676-85
PMID:34250083 - Barcode sequencing and a high-throughput assay for chronological lifespan uncover ageing-associated genes in fission yeast.
Romila CA et al. Microb Cell 2021 Jul 05;8(7):146-160
PMID:39476757 - Characterization of Ksg1 protein kinase-dependent phosphoproteome in the fission yeast S. pombe.
Cipak L et al. Biochem Biophys Res Commun 2024 Oct 25;736:150895