PomBase home

protein coding gene - cut4 (SPBC106.09) - anaphase-promoting complex platform subcomplex scaffold subunit Apc1

Gene summary

Standard name
cut4
Systematic ID
SPBC106.09
Product
anaphase-promoting complex platform subcomplex scaffold subunit Apc1
Organism
Schizosaccharomyces pombe (fission yeast)
Synonyms
apc1
UniProt ID
Q9URV2
ORFeome ID
48/48G04
Characterisation status
biological role published
Feature type
mRNA gene
Genomic location
chromosome II: 390910..396037 forward strand

Annotation

Disease association

MONDO:0016368 - Rothmund-Thomson syndrome type 1

References:

GO biological process

GO:0031145 - anaphase-promoting complex-dependent catabolic process

References:

GO:0007091 - metaphase/anaphase transition of mitotic cell cycle

References:

GO:0051306 - mitotic sister chromatid separation

References:

GO cellular component

GO:0005680 - anaphase-promoting complex

References:

GO:0005829 - cytosol

References:

GO:0005634 - nucleus

References:

GO:0005721 - pericentric heterochromatin

References:

GO molecular function

GO:0060090 - molecular adaptor activity

References:

GO:0070628 - proteasome binding

References:

Modification

MOD:00046 - O-phospho-L-serine

References:

MOD:00047 - O-phospho-L-threonine

References:

Multi-locus phenotype

FYPO:0001324 - decreased protein level during vegetative growth

References:

Genotypes:

FYPO:0001355 - decreased vegetative cell population growth

References:

Genotypes:

FYPO:0002061 - inviable vegetative cell population

References:

Genotypes:

FYPO:0007914 - mitotic sister chromatid separation during metaphase

References:

Genotypes:

Qualitative gene expression

PomGeneEx:0000018 - protein level increased

References:

PomGeneEx:0000011 - RNA level increased

References:

Quantitative gene expression

PBO:0006310 - protein level

References:

PBO:0011963 - RNA level

References:

Single locus phenotype

FYPO:0000620 - abnormal cell cycle arrest in mitotic metaphase

References:

Genotypes:

FYPO:0002346 - abnormal chromatin silencing at centromere outer repeat

References:

Genotypes:

FYPO:0000206 - abnormal chromatin silencing at rDNA

References:

Genotypes:

FYPO:0000156 - abnormal chromatin silencing at silent mating-type cassette

References:

Genotypes:

FYPO:0000059 - abnormal mitotic cell cycle

References:

Genotypes:

FYPO:0000229 - cut

References:

Genotypes:

FYPO:0004418 - cut cell with decreased poly(A)+ mRNA export from nucleus

References:

Genotypes:

FYPO:0003165 - cut with abnormal chromosome segregation

References:

Genotypes:

FYPO:0003352 - decreased DNA double-strand break formation at mating-type locus

References:

Genotypes:

FYPO:0000877 - decreased histone H3-K9 dimethylation at centromere during vegetative growth

References:

Genotypes:

FYPO:0002355 - decreased histone H3-K9 dimethylation at silent mating-type cassette during vegetative growth

References:

Genotypes:

FYPO:0003681 - decreased protein level at centromere outer repeat heterochromatin

References:

Genotypes:

FYPO:0005102 - decreased protein level at silent mating-type cassette

References:

Genotypes:

FYPO:0004332 - delayed onset of protein degradation during mitosis

References:

Genotypes:

FYPO:0001861 - increased minichromosome loss upon segregation during vegetative growth

References:

Genotypes:

FYPO:0001327 - increased protein level during vegetative growth

References:

Genotypes:

FYPO:0001490 - inviable elongated vegetative cell

References:

Genotypes:

FYPO:0002061 - inviable vegetative cell population

References:

Genotypes:

FYPO:0001511 - inviable vegetative cell, abnormal cell shape, normal cell size

References:

Genotypes:

FYPO:0001886 - meiotic cell cycle entry and sporulation in haploid

References:

Genotypes:

FYPO:0003570 - normal attachment of spindle microtubules to kinetochore

References:

Genotypes:

FYPO:0000096 - sensitive to cadmium

References:

Genotypes:

FYPO:0001245 - sensitive to cobalt

References:

Genotypes:

FYPO:0000751 - sensitive to nickel

References:

Genotypes:

FYPO:0001234 - slow vegetative cell population growth

References:

Genotypes:

FYPO:0002060 - viable vegetative cell population

References:

Genotypes:

Taxonomic conservation

PBO:0011065 - conserved in eukaryotes

PBO:0011071 - conserved in eukaryotes only

PBO:0011064 - conserved in fungi

PBO:0011069 - conserved in metazoa

PBO:0011070 - conserved in vertebrates

PBO:0006222 - predominantly single copy (one to one)

Protein features

IDNameInterPro nameDB name
PF21282APC1_3rdApc1_3rdPfam
PF27562APC1_4thTPR_APC1Pfam
PF29300APC1_5thAPC1_5thPfam
G3DSA:1.25.10.10:FF:000400CATH-FunFam
G3DSA:1.25.10.10ARM-likeCATH-Gene3D
PTHR12827APC1PANTHER
mobidb-lite-Disorderdisorder_predictionMobiDB-Disorder
mobidb-lite-Polyampholytedisorder_predictionMobiDB-Polyampholyte

Orthologs

References / Literature

PMID:16823372 - ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe.
Matsuyama A et al. Nat Biotechnol 2006 Jul;24(7):841-7
PMID:38780300 - Nitrogen availability is important for preventing catastrophic mitosis in fission yeast.
Zemlianski V et al. J Cell Sci 2024 May 23;
PMID:39367033 - Quantitative proteomics and phosphoproteomics profiling of meiotic divisions in the fission yeast Schizosaccharomyces pombe.
Sivakova B et al. Sci Rep 2024 Oct 04;14(1):23105
PMID:15507118 - An interactive gene network for securin-separase, condensin, cohesin, Dis1/Mtc1 and histones constructed by mass transformation.
Yuasa T et al. Genes Cells 2004 Nov;9(11):1069-82
PMID:12242294 - The mal2p protein is an essential component of the fission yeast centromere.
Jin QW et al. Mol Cell Biol 2002 Oct;22(20):7168-83
PMID:19117951 - Interaction of APC/C-E3 ligase with Swi6/HP1 and Clr4/Suv39 in heterochromatin assembly in fission yeast.
Dubey RN et al. J Biol Chem 2009 Mar 13;284(11):7165-76
PMID:19756689 - SUMOylation is required for normal development of linear elements and wild-type meiotic recombination in Schizosaccharomyces pombe.
Spirek M et al. Chromosoma 2010 Feb;119(1):59-72
GO_REF:0000111 - Gene Ontology annotations Inferred by Curator (IC) using at least one Inferred by Sequence Similarity (ISS) annotation to support the inference
PMID:12653962 - A brute force postgenome approach to identify temperature-sensitive mutations that negatively interact with separase and securin plasmids.
Matsumura T et al. Genes Cells 2003 Apr;8(4):341-55
PMID:16950791 - Role of Hcn1 and its phosphorylation in fission yeast anaphase-promoting complex/cyclosome function.
Yoon HJ et al. J Biol Chem 2006 Oct 27;281(43):32284-93
PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.
Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623
PMID:25720772 - Quantitative phosphoproteomics reveals pathways for coordination of cell growth and division by the conserved fission yeast kinase pom1.
Kettenbach AN et al. Mol Cell Proteomics 2015 May;14(5):1275-87
PMID:11950879 - Control of localization of a spindle checkpoint protein, Mad2, in fission yeast.
Ikui AE et al. J Cell Sci 2002 Apr 15;115(Pt 8):1603-10
PMID:12161753 - The transcriptional program of meiosis and sporulation in fission yeast.
Mata J et al. Nat Genet 2002 Sep;32(1):143-7
PMID:18354085 - Diminishing HDACs by drugs or mutations promotes normal or abnormal sister chromatid separation by affecting APC/C and adherin.
Kimata Y et al. J Cell Sci 2008 Apr 01;121(Pt 7):1107-18
PMID:30355493 - Expanded Interactome of the Intrinsically Disordered Protein Dss1.
Schenstrøm SM et al. Cell Rep 2018 Oct 23;25(4):862-870
PB_REF:0000006 - Disease associations from Monarch via human-pombe orthologs
PMID:8918880 - 20S cyclosome complex formation and proteolytic activity inhibited by the cAMP/PKA pathway.
Yamashita YM et al. Nature 1996 Nov 21;384(6606):276-9
PMID:16453724 - Isolation and characterization of Schizosaccharomyces pombe cutmutants that block nuclear division but not cytokinesis.
Hirano T et al. EMBO J 1986 Nov;5(11):2973-9
PMID:30726745 - Fission Yeast NDR/LATS Kinase Orb6 Regulates Exocytosis via Phosphorylation of the Exocyst Complex.
Tay YD et al. Cell Rep 2019 Feb 05;26(6):1654-1667.e7
PMID:11058086 - High dosage expression of a zinc finger protein, Grt1, suppresses a mutant of fission yeast slp1(+), a homolog of CDC20/p55CDC/Fizzy.
Yamada HY et al. J Cell Sci 2000 Nov;113 ( Pt 22):3989-99
PMID:10398680 - Proper metaphase spindle length is determined by centromere proteins Mis12 and Mis6 required for faithful chromosome segregation.
Goshima G et al. Genes Dev 1999 Jul 01;13(13):1664-77
PMID:10582241 - Control of metaphase-anaphase progression by proteolysis: cyclosome function regulated by the protein kinase A pathway, ubiquitination and localization.
Yanagida M et al. Philos Trans R Soc Lond B Biol Sci 1999 Sep 29;354(1389):1559-69; discussion 1569-70
GO_REF:0000033 - Annotation inferences using phylogenetic trees
PMID:15161942 - The nucleolus is involved in mRNA export from the nucleus in fission yeast.
Ideue T et al. J Cell Sci 2004 Jun 15;117(Pt 14):2887-95
PMID:12618370 - Schizosaccharomyces pombe essential genes: a pilot study.
Decottignies A et al. Genome Res 2003 Mar;13(3):399-406
PMID:18556659 - The spindle checkpoint functions of Mad3 and Mad2 depend on a Mad3 KEN box-mediated interaction with Cdc20-anaphase-promoting complex (APC/C).
Sczaniecka M et al. J Biol Chem 2008 Aug 22;283(34):23039-47
PMID:15060174 - Swm1/Apc13 is an evolutionarily conserved subunit of the anaphase-promoting complex stabilizing the association of Cdc16 and Cdc27.
Schwickart M et al. Mol Cell Biol 2004 Apr;24(8):3562-76
PMID:39476757 - Characterization of Ksg1 protein kinase-dependent phosphoproteome in the fission yeast S. pombe.
Cipak L et al. Biochem Biophys Res Commun 2024 Oct 25;736:150895
PMID:12477395 - Proteomics analysis identifies new components of the fission and budding yeast anaphase-promoting complexes.
Yoon HJ et al. Curr Biol 2002 Dec 10;12(23):2048-54
PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.
Marguerat S et al. Cell 2012 Oct 26;151(3):671-83
PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.
Hayles J et al. Open Biol 2013 May 22;3(5):130053
PMID:24948786 - Histone H2B ubiquitination promotes the function of the anaphase-promoting complex/cyclosome in Schizosaccharomyces pombe.
Elmore ZC et al. G3 (Bethesda) 2014 Jun 19;4(8):1529-38
PMID:9461438 - Fission yeast Slp1: an effector of the Mad2-dependent spindle checkpoint.
Kim SH et al. Science 1998 Feb 13;279(5353):1045-7
PMID:11084332 - Cut8, essential for anaphase, controls localization of 26S proteasome, facilitating destruction of cyclin and Cut2.
Tatebe H et al. Curr Biol 2000 Nov 02;10(21):1329-38
PMID:21511999 - Comparative functional genomics of the fission yeasts.
Rhind N et al. Science 2011 May 20;332(6032):930-6
PMID:12615927 - Interaction of the anaphase-promoting complex/cyclosome and proteasome protein complexes with multiubiquitin chain-binding proteins.
Seeger M et al. J Biol Chem 2003 May 09;278(19):16791-6
PMID:11683390 - In vivo localisation of fission yeast cyclin-dependent kinase cdc2p and cyclin B cdc13p during mitosis and meiosis.
Decottignies A et al. J Cell Sci 2001 Jul;114(Pt 14):2627-40
PMID:16904908 - Cdc48 is required for the stability of Cut1/separase in mitotic anaphase.
Ikai N et al. J Struct Biol 2006 Oct;156(1):50-61
PMID:10526233 - Fission yeast APC/cyclosome subunits, Cut20/Apc4 and Cut23/Apc8, in regulating metaphase-anaphase progression and cellular stress responses.
Yamashita YM et al. Genes Cells 1999 Aug;4(8):445-63
PMID:18082611 - Structural organization of the anaphase-promoting complex bound to the mitotic activator Slp1.
Ohi MD et al. Mol Cell 2007 Dec 14;28(5):871-85
PMID:29996109 - Quantitative Phosphoproteomics Reveals the Signaling Dynamics of Cell-Cycle Kinases in the Fission Yeast Schizosaccharomyces pombe.
Swaffer MP et al. Cell Rep 2018 Jul 10;24(2):503-514
PMID:31719112 - Checkpoint Regulation of Nuclear Tos4 Defines S Phase Arrest in Fission Yeast.
Kim SM et al. G3 (Bethesda) 2020 Jan 07;10(1):255-266
PMID:28366744 - Fission Yeast Apc15 Stabilizes MCC-Cdc20-APC/C Complexes, Ensuring Efficient Cdc20 Ubiquitination and Checkpoint Arrest.
May KM et al. Curr Biol 2017 Apr 24;27(8):1221-1228