protein coding gene - cnp1 (SPBC1105.17) - centromere-specific histone H3 CENP-A

Gene summary

Standard name

cnp1

Systematic ID

SPBC1105.17

Product

centromere-specific histone H3 CENP-A

Organism

Schizosaccharomyces pombe (fission yeast)

Synonyms

sim2

UniProt ID

Q9Y812

ORFeome ID

02/02E03

Characterisation status

biological role published

Feature type

mRNA gene

Genomic location

chromosome II: 3538504..3540163 forward strand

Annotation

Protein features

Orthologs

• CENPA (HGNC:1851) Homo sapiens
• cnp1 (SJAG_00307) Schizosaccharomyces japonicus
• CSE4 (YKL049C) Saccharomyces cerevisiae

References / Literature

PMID:22681890 - Hierarchical modularity and the evolution of genetic interactomes across species.

Ryan CJ et al. Mol Cell 2012 Jun 08;46(5):691-704

PMID:31883795 - Positioning Heterochromatin at the Nuclear Periphery Suppresses Histone Turnover to Promote Epigenetic Inheritance.

Holla S et al. Cell 2020 Jan 09;180(1):150-164.e15

PMID:16997270 - Fission yeast Mcl1 interacts with SCF(Pof3) and is required for centromere formation.

Mamnun YM et al. Biochem Biophys Res Commun 2006 Nov 10;350(1):125-30

PMID:34849791 - Perturbation of kinetochore function using GFP-binding protein in fission yeast.

Deng DJ et al. G3 (Bethesda) 2021 Oct 19;11(11)

PMID:19217404 - Fission yeast Scm3: A CENP-A receptor required for integrity of subkinetochore chromatin.

Pidoux AL et al. Mol Cell 2009 Feb 13;33(3):299-311

PMID:12535531 - A cell cycle-regulated GATA factor promotes centromeric localization of CENP-A in fission yeast.

Chen ES et al. Mol Cell 2003 Jan;11(1):175-87

PMID:27334362 - Inner nuclear membrane protein Lem2 augments heterochromatin formation in response to nutritional conditions.

Tange Y et al. Genes Cells 2016 Aug;21(8):812-32

PMID:25669599 - Fitness profiling links topoisomerase II regulation of centromeric integrity to doxorubicin resistance in fission yeast.

Nguyen TT et al. Sci Rep 2015 Feb 11;5:8400

GO_REF:0000051 - S. pombe keyword mapping

PMID:16688222 - Histone H2B mutations in inner region affect ubiquitination, centromere function, silencing and chromosome segregation.

Maruyama T et al. EMBO J 2006 Jun 07;25(11):2420-31

PMID:22084306 - CENP-A exceeds microtubule attachment sites in centromere clusters of both budding and fission yeast.

Coffman VC et al. J Cell Biol 2011 Nov 14;195(4):563-72

PMID:25619765 - The CENP-A N-tail confers epigenetic stability to centromeres via the CENP-T branch of the CCAN in fission yeast.

Folco HD et al. Curr Biol 2015 Feb 02;25(3):348-356

PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.

Marguerat S et al. Cell 2012 Oct 26;151(3):671-83

PMID:12161753 - The transcriptional program of meiosis and sporulation in fission yeast.

Mata J et al. Nat Genet 2002 Sep;32(1):143-7

PMID:16823372 - ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe.

Matsuyama A et al. Nat Biotechnol 2006 Jul;24(7):841-7

PMID:19217403 - Fission yeast Scm3 mediates stable assembly of Cnp1/CENP-A into centromeric chromatin.

Williams JS et al. Mol Cell 2009 Feb 13;33(3):287-98

PMID:12773390 - The role of Ppe1/PP6 phosphatase for equal chromosome segregation in fission yeast kinetochore.

Goshima G et al. EMBO J 2003 Jun 02;22(11):2752-63

PMID:23166349 - Csi1 links centromeres to the nuclear envelope for centromere clustering.

Hou H et al. J Cell Biol 2012 Nov 26;199(5):735-44

PMID:24789708 - Eic1 links Mis18 with the CCAN/Mis6/Ctf19 complex to promote CENP-A assembly.

Subramanian L et al. Open Biol 2014 Apr 30;4(4):140043

PMID:19758558 - CENP-C functions as a scaffold for effectors with essential kinetochore functions in mitosis and meiosis.

Tanaka K et al. Dev Cell 2009 Sep;17(3):334-43

PMID:26275423 - Inner Kinetochore Protein Interactions with Regional Centromeres of Fission Yeast.

Thakur J et al. Genetics 2015 Oct;201(2):543-61

PMID:27666591 - Ccp1 Homodimer Mediates Chromatin Integrity by Antagonizing CENP-A Loading.

Dong Q et al. Mol Cell 2016 Oct 06;64(1):79-91

PMID:17035632 - Reconstruction of the kinetochore during meiosis in fission yeast Schizosaccharomyces pombe.

Hayashi A et al. Mol Biol Cell 2006 Dec;17(12):5173-84

PMID:35970865 - The Mis6 inner kinetochore subcomplex maintains CENP-A nucleosomes against centromeric non-coding transcription during mitosis.

Hirai H et al. Commun Biol 2022 Aug 15;5(1):818

PMID:25375240 - The kinetochore protein Kis1/Eic1/Mis19 ensures the integrity of mitotic spindles through maintenance of kinetochore factors Mis6/CENP-I and CENP-A.

Hirai H et al. PLoS One 2014;9(11):e111905

PMID:26369364 - Mutation of histone H3 serine 86 disrupts GATA factor Ams2 expression and precise chromosome segregation in fission yeast.

Lim KK et al. Sci Rep 2015 Sep 15;5:14064

GO_REF:0000033 - Annotation inferences using phylogenetic trees

PMID:38084929 - The cysteine-rich domain in CENP-A chaperone Scm3HJURP ensures centromere targeting and kinetochore integrity.

Folco HD et al. Nucleic Acids Res 2023 Dec 12;

PMID:18493607 - A DNA polymerase alpha accessory protein, Mcl1, is required for propagation of centromere structures in fission yeast.

Natsume T et al. PLoS One 2008 May 21;3(5):e2221

PMID:15908586 - The CHD remodeling factor Hrp1 stimulates CENP-A loading to centromeres.

Walfridsson J et al. Nucleic Acids Res 2005;33(9):2868-79

PMID:10864871 - Requirement of Mis6 centromere connector for localizing a CENP-A-like protein in fission yeast.

Takahashi K et al. Science 2000 Jun 23;288(5474):2215-9

PMID:12719471 - Sim4: a novel fission yeast kinetochore protein required for centromeric silencing and chromosome segregation.

Pidoux AL et al. J Cell Biol 2003 Apr 28;161(2):295-307

PMID:21324894 - Nuclear protein quality is regulated by the ubiquitin-proteasome system through the activity of Ubc4 and San1 in fission yeast.

Matsuo Y et al. J Biol Chem 2011 Apr 15;286(15):13775-90

PMID:30635289 - Cell Cycle-Regulated Transcription of CENP-A by the MBF Complex Ensures Optimal Level of CENP-A for Centromere Formation.

Aristizabal-Corrales D et al. Genetics 2019 Mar;211(3):861-875

GO_REF:0000002 - Comments

PMID:41453208 - Cnp1 N-terminal dynamics regulate L1 loop recognition by Mis15 to orchestrate kinetochore assembly in Schizosaccharomyces pombe.

Xiong Y et al. J Mol Cell Biol 2025 Dec 26;

PMID:28827290 - Kinetochore Components Required for Centromeric Chromatin Assembly Are Impacted by Msc1 in Schizosaccharomyces pombe .

Gao C et al. Genetics 2017 Oct;207(2):559-569

PMID:23267073 - Cell cycle-dependent deposition of CENP-A requires the Dos1/2-Cdc20 complex.

Gonzalez M et al. Proc Natl Acad Sci U S A 2013 Jan 08;110(2):606-11

PMID:28977643 - Regulation of mitotic recombination between DNA repeats in centromeres.

Zafar F et al. Nucleic Acids Res 2017 Nov 02;45(19):11222-11235

PMID:19443688 - Diverse roles of HP1 proteins in heterochromatin assembly and functions in fission yeast.

Fischer T et al. Proc Natl Acad Sci U S A 2009 Jun 02;106(22):8998-9003

PMID:24774534 - Schizosaccharomyces pombe centromere protein Mis19 links Mis16 and Mis18 to recruit CENP-A through interacting with NMD factors and the SWI/SNF complex.

Hayashi T et al. Genes Cells 2014 Jul;19(7):541-54

PMID:22540037 - Predicting the fission yeast protein interaction network.

Pancaldi V et al. G3 (Bethesda) 2012 Apr;2(4):453-67

PMID:27901072 - Characterisation of functional domains in fission yeast Ams2 that are required for core histone gene transcription.

Takayama Y et al. Sci Rep 2016 Nov 30;6:38111

PMID:29194511 - Prolyl isomerization of the CENP-A N-terminus regulates centromeric integrity in fission yeast.

Tan HL et al. Nucleic Acids Res 2018 Feb 16;46(3):1167-1179

PMID:11553715 - The domain structure of centromeres is conserved from fission yeast to humans.

Kniola B et al. Mol Biol Cell 2001 Sep;12(9):2767-75

PMID:18158900 - A NASP (N1/N2)-related protein, Sim3, binds CENP-A and is required for its deposition at fission yeast centromeres.

Dunleavy EM et al. Mol Cell 2007 Dec 28;28(6):1029-44

PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.

Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623

PMID:23314747 - Myb-domain protein Teb1 controls histone levels and centromere assembly in fission yeast.

Valente LP et al. EMBO J 2013 Feb 06;32(3):450-60

PMID:28974540 - The fission yeast nucleoporin Alm1 is required for proteasomal degradation of kinetochore components.

Salas-Pino S et al. J Cell Biol 2017 Nov 06;216(11):3591-3608

PMID:15930132 - Spindle checkpoint signaling requires the mis6 kinetochore subcomplex, which interacts with mad2 and mitotic spindles.

Saitoh S et al. Mol Biol Cell 2005 Aug;16(8):3666-77

PMID:30503616 - Centromere DNA Destabilizes H3 Nucleosomes to Promote CENP-A Deposition during the Cell Cycle.

Shukla M et al. Curr Biol 2018 Dec 17;28(24):3924-3936.e4

PMID:39367033 - Quantitative proteomics and phosphoproteomics profiling of meiotic divisions in the fission yeast Schizosaccharomyces pombe.

Sivakova B et al. Sci Rep 2024 Oct 04;14(1):23105

PMID:16079915 - The DASH complex and Klp5/Klp6 kinesin coordinate bipolar chromosome attachment in fission yeast.

Sanchez-Perez I et al. EMBO J 2005 Aug 17;24(16):2931-43

PMID:17947424 - Msc1 acts through histone H2A.Z to promote chromosome stability in Schizosaccharomyces pombe.

Ahmed S et al. Genetics 2007 Nov;177(3):1487-97

PMID:30089114 - Heterochromatin and RNAi regulate centromeres by protecting CENP-A from ubiquitin-mediated degradation.

Yang J et al. PLoS Genet 2018 Aug;14(8):e1007572

PMID:18077559 - Biphasic incorporation of centromeric histone CENP-A in fission yeast.

Takayama Y et al. Mol Biol Cell 2008 Feb;19(2):682-90

PMID:29899117 - Ccp1 modulates epigenetic stability at centromeres and affects heterochromatin distribution in Schizosaccharomyces pombe .

Lu M et al. J Biol Chem 2018 Aug 03;293(31):12068-12080

PMID:36200823 - The chromatin remodeler RSC prevents ectopic CENP-A propagation into pericentromeric heterochromatin at the chromatin boundary.

Tsunemine S et al. Nucleic Acids Res 2022 Oct 28;50(19):10914-10928

PMID:24710126 - The 19S proteasome subunit Rpt3 regulates distribution of CENP-A by associating with centromeric chromatin.

Kitagawa T et al. Nat Commun 2014 Apr 07;5:3597

PMID:34810257 - Ccp1-Ndc80 switch at the N terminus of CENP-T regulates kinetochore assembly.

Dong Q et al. Proc Natl Acad Sci U S A 2021 Nov 30;118(48)

PMID:17352737 - The fission yeast DASH complex is essential for satisfying the spindle assembly checkpoint induced by defects in the inner-kinetochore proteins.

Kobayashi Y et al. Genes Cells 2007 Mar;12(3):311-28

PMID:32318758 - Centromere targeting of Mis18 requires the interaction with DNA and H2A-H2B in fission yeast.

Zhang M et al. Cell Mol Life Sci 2021 Jan;78(1):373-384

PMID:28228545 - Ablation of RNA interference and retrotransposons accompany acquisition and evolution of transposases to heterochromatin protein CENPB.

Upadhyay U et al. Mol Biol Cell 2017 Apr 15;28(8):1132-1146

PMID:30967422 - Negative Regulation of the Mis17-Mis6 Centromere Complex by mRNA Decay Pathway and EKC/KEOPS Complex in Schizosaccharomyces pombe .

Xu X et al. G3 (Bethesda) 2019 Jun 05;9(6):1815-1823

PMID:16537923 - Sterol regulatory element binding protein is a principal regulator of anaerobic gene expression in fission yeast.

Todd BL et al. Mol Cell Biol 2006 Apr;26(7):2817-31

PMID:23089178 - Sim3 shares some common roles with the histone chaperone Asf1 in fission yeast.

Tanae K et al. FEBS Lett 2012 Nov 30;586(23):4190-6

PMID:25298518 - Ectopic centromere nucleation by CENP--a in fission yeast.

Gonzalez M et al. Genetics 2014 Dec;198(4):1433-46

PMID:17452352 - Differential regulation of repeated histone genes during the fission yeast cell cycle.

Takayama Y et al. Nucleic Acids Res 2007;35(10):3223-37

GO_REF:0000050 - Manual transfer of GO annotation data to genes by curator judgment of sequence model

PMID:18362178 - Dissection of the essential steps for condensin accumulation at kinetochores and rDNAs during fission yeast mitosis.

Nakazawa N et al. J Cell Biol 2008 Mar 24;180(6):1115-31

PMID:39786922 - The fission yeast SUMO-targeted ubiquitin ligase Slx8 functionally associates with clustered centromeres and the silent mating-type region at the nuclear periphery.

Chakraborty S et al. Biol Open 2024 Dec 15;13(12)

PMID:26832414 - Condensin Promotes Position Effects within Tandem DNA Repeats via the RITS Complex.

He H et al. Cell Rep 2016 Feb 09;14(5):1018-1024

PMID:21511999 - Comparative functional genomics of the fission yeasts.

Rhind N et al. Science 2011 May 20;332(6032):930-6

PMID:38526189 - Cdc48 and its co-factor Ufd1 extract CENP-A from centromeric chromatin and can induce chromosome elimination in the fission yeast Schizosaccharomyces pombe.

Nakase Y et al. Biol Open 2024 Mar 25;

PMID:15897182 - Two distinct pathways responsible for the loading of CENP-A to centromeres in the fission yeast cell cycle.

Takahashi K et al. Philos Trans R Soc Lond B Biol Sci 2005 Mar 29;360(1455):595-606; discussion 606-7

PMID:17677001 - Plasticity of fission yeast CENP-A chromatin driven by relative levels of histone H3 and H4.

Castillo AG et al. PLoS Genet 2007 Jul;3(7):e121

PMID:31000521 - Overlapping Roles in Chromosome Segregation for Heterochromatin Protein 1 (Swi6) and DDK in Schizosaccharomyces pombe .

Shen KF et al. Genetics 2019 Jun;212(2):417-430

PMID:15369671 - Mis16 and Mis18 are required for CENP-A loading and histone deacetylation at centromeres.

Hayashi T et al. Cell 2004 Sep 17;118(6):715-29

PMID:23936074 - Telomeric repeats facilitate CENP-A(Cnp1) incorporation via telomere binding proteins.

Castillo AG et al. PLoS One 2013;8(7):e69673

PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.

Hayles J et al. Open Biol 2013 May 22;3(5):130053

PMID:23028377 - Factors that promote H3 chromatin integrity during transcription prevent promiscuous deposition of CENP-A(Cnp1) in fission yeast.

Choi ES et al. PLoS Genet 2012 Sep;8(9):e1002985

PMID:28904333 - The Ino80 complex mediates epigenetic centromere propagation via active removal of histone H3.

Choi ES et al. Nat Commun 2017 Sep 13;8(1):529