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protein coding gene - ubc4 (SPBC119.02) - ubiquitin conjugating enzyme E2 for APC and SCF Ubc4/UbcP1

Gene summary

Standard name
ubc4
Systematic ID
SPBC119.02
Product
ubiquitin conjugating enzyme E2 for APC and SCF Ubc4/UbcP1
Organism
Schizosaccharomyces pombe (fission yeast)
Synonyms
ubcP1
UniProt ID
P46595
ORFeome ID
32/32A10
Characterisation status
biological role published
Feature type
mRNA gene
Genomic location
chromosome II: 715285..716311 forward strand

Annotation

PBO:0000272 - 6.3.2.19

GO biological process

GO:0045842 - positive regulation of mitotic metaphase/anaphase transition

References:

GO:0031146 - SCF-dependent proteasomal ubiquitin-dependent protein catabolic process

References:

GO:0032933 - SREBP signaling pathway

References:

GO:0006511 - ubiquitin-dependent protein catabolic process

References:

GO cellular component

GO:0005737 - cytoplasm

References:

GO:0005829 - cytosol

References:

GO:0005634 - nucleus

References:

GO molecular function

GO:0005515 - protein binding

References:

GO:0061631 - ubiquitin conjugating enzyme activity

References:

Modification

MOD:01149 - sumoylated lysine

References:

MOD:01148 - ubiquitinylated lysine

References:

Multi-locus phenotype

FYPO:0001740 - increased gross chromosomal rearrangement

References:

Genotypes:

Quantitative gene expression

PBO:0006310 - protein level

References:

PBO:0011963 - RNA level

References:

Single locus phenotype

FYPO:0000620 - abnormal cell cycle arrest in mitotic metaphase

References:

Genotypes:

FYPO:0000059 - abnormal mitotic cell cycle

References:

Genotypes:

FYPO:0001910 - abnormal protein glycosylation during vegetative growth

References:

Genotypes:

FYPO:0000912 - abolished protein ubiquitination during vegetative growth

References:

Genotypes:

FYPO:0003165 - cut with abnormal chromosome segregation

References:

Genotypes:

FYPO:0003585 - decreased anaphase-promoting complex-dependent protein catabolic process

References:

Genotypes:

FYPO:0000082 - decreased cell population growth at high temperature

References:

Genotypes:

FYPO:0006810 - decreased gross chromosomal rearrangement

References:

Genotypes:

FYPO:0004198 - decreased protein degradation during mitosis

References:

Genotypes:

FYPO:0003935 - decreased protein localization to Golgi apparatus, with protein mislocalized to endoplasmic reticulum

References:

Genotypes:

FYPO:0004102 - decreased protein polyubiquitination during vegetative growth

References:

Genotypes:

FYPO:0001422 - decreased protein processing during vegetative growth

References:

Genotypes:

FYPO:0002768 - decreased protein ubiquitination during vegetative growth

References:

Genotypes:

FYPO:0001740 - increased gross chromosomal rearrangement

References:

Genotypes:

FYPO:0005371 - increased linear minichromosome loss during vegetative growth

References:

Genotypes:

FYPO:0004108 - increased protein level during mitotic G1 phase

References:

Genotypes:

FYPO:0001991 - inviable after spore germination, without cell division

References:

Genotypes:

FYPO:0001489 - inviable vegetative cell

References:

Genotypes:

FYPO:0002061 - inviable vegetative cell population

References:

Genotypes:

FYPO:0003758 - mitotic spindle elongation without chromosome separation

References:

Genotypes:

FYPO:0003594 - normal anaphase-promoting complex-dependent protein catabolic process

References:

Genotypes:

FYPO:0002448 - normal Dsc complex assembly

References:

Genotypes:

FYPO:0002635 - normal protein ubiquitination during vegetative growth

References:

Genotypes:

FYPO:0001357 - normal vegetative cell population growth

References:

Genotypes:

FYPO:0001492 - viable elongated vegetative cell

References:

Genotypes:

Taxonomic conservation

PBO:0011065 - conserved in eukaryotes

PBO:0011071 - conserved in eukaryotes only

PBO:0011064 - conserved in fungi

PBO:0011069 - conserved in metazoa

PBO:0011070 - conserved in vertebrates

Protein features

IDNameInterPro nameDB name
PF00179UQ_conUBCPFAM
cd23792UBCc_UBE2DCDD
PS00183UBC_1UBQ-conjugating_ASPROSITE_PATTERNS
PS50127UBC_2UBCPROSITE_PROFILES
SM00212ubc_7SMART
G3DSA:3.10.110.10:FF:000010FUNFAM
SSF54495UBC-likeUBQ-conjugating_enzyme/RWDSUPERFAMILY
G3DSA:3.10.110.10Ubiquitin Conjugating EnzymeUBQ-conjugating_enzyme/RWDGENE3D
PTHR24068UBIQUITIN-CONJUGATING ENZYME E2PANTHER

Orthologs

References / Literature

PMID:9154838 - A ubiquitin-conjugating enzyme in fission yeast that is essential for the onset of anaphase in mitosis.
Osaka F et al. Mol Cell Biol 1997 Jun;17(6):3388-97
PMID:26882497 - Mps1Mph1 Kinase Phosphorylates Mad3 to Inhibit Cdc20Slp1-APC/C and Maintain Spindle Checkpoint Arrests.
Zich J et al. PLoS Genet 2016 Feb;12(2):e1005834
PMID:24497846 - A chaperone-assisted degradation pathway targets kinetochore proteins to ensure genome stability.
Kriegenburg F et al. PLoS Genet 2014 Jan;10(1):e1004140
PMID:23416107 - Structure of a ubiquitin E1-E2 complex: insights to E1-E2 thioester transfer.
Olsen SK et al. Mol Cell 2013 Mar 07;49(5):884-96
PMID:40015273 - A comprehensive Schizosaccharomyces pombe atlas of physical transcription factor interactions with proteins and chromatin.
Skribbe M et al. Mol Cell 2025 Feb 19;
PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.
Hayles J et al. Open Biol 2013 May 22;3(5):130053
PMID:25165823 - Multiple regulation of Rad51-mediated homologous recombination by fission yeast Fbh1.
Tsutsui Y et al. PLoS Genet 2014 Aug;10(8):e1004542
PMID:12724408 - Two ubiquitin-conjugating enzymes, UbcP1/Ubc4 and UbcP4/Ubc11, have distinct functions for ubiquitination of mitotic cyclin.
Seino H et al. Mol Cell Biol 2003 May;23(10):3497-505
PMID:25918164 - Endoplasmic Reticulum Exit of Golgi-resident Defective for SREBP Cleavage (Dsc) E3 Ligase Complex Requires Its Activity.
Raychaudhuri S et al. J Biol Chem 2015 Jun 05;290(23):14430-40
PMID:34292936 - Fission yeast Rad8/HLTF facilitates Rad52-dependent chromosomal rearrangements through PCNA lysine 107 ubiquitination.
Su J et al. PLoS Genet 2021 Jul;17(7):e1009671
PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.
Marguerat S et al. Cell 2012 Oct 26;151(3):671-83
PMID:21324894 - Nuclear protein quality is regulated by the ubiquitin-proteasome system through the activity of Ubc4 and San1 in fission yeast.
Matsuo Y et al. J Biol Chem 2011 Apr 15;286(15):13775-90
PMID:21504829 - Yeast SREBP cleavage activation requires the Golgi Dsc E3 ligase complex.
Stewart EV et al. Mol Cell 2011 Apr 22;42(2):160-71
PMID:23442800 - An E2 enzyme Ubc11 is required for ubiquitination of Slp1/Cdc20 and spindle checkpoint silencing in fission yeast.
Horikoshi Y et al. Cell Cycle 2013 Mar 15;12(6):961-71
PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.
Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623
PMID:24454826 - E3 ubiquitin ligase Pub1 is implicated in endocytosis of a GPI-anchored protein Ecm33 in fission yeast.
Fang Y et al. PLoS One 2014;9(1):e85238
PMID:26537787 - Targeting of SUMO substrates to a Cdc48-Ufd1-Npl4 segregase and STUbL pathway in fission yeast.
Køhler JB et al. Nat Commun 2015 Nov 05;6:8827
PMID:28552615 - SUMO-Targeted DNA Translocase Rrp2 Protects the Genome from Top2-Induced DNA Damage.
Wei Y et al. Mol Cell 2017 Jun 01;66(5):581-596.e6
PMID:39143218 - Structural basis for transthiolation intermediates in the ubiquitin pathway.
Kochańczyk T et al. Nature 2024 Sep;633(8028):216-223
PMID:8972853 - Fission yeast genes which disrupt mitotic chromosome segregation when overexpressed.
Javerzat JP et al. Nucleic Acids Res 1996 Dec 01;24(23):4676-83
PMID:26412298 - A Degenerate Cohort of Yeast Membrane Trafficking DUBs Mediates Cell Polarity and Survival.
Beckley JR et al. Mol Cell Proteomics 2015 Dec;14(12):3132-41
PMID:23729666 - Structural requirements for sterol regulatory element-binding protein (SREBP) cleavage in fission yeast.
Chong R et al. J Biol Chem 2013 Jul 12;288(28):20351-60
PMID:24763107 - Absolute proteome and phosphoproteome dynamics during the cell cycle of Schizosaccharomyces pombe (Fission Yeast).
Carpy A et al. Mol Cell Proteomics 2014 Aug;13(8):1925-36
PMID:28218250 - Chromatin remodeller Fun30 Fft3 induces nucleosome disassembly to facilitate RNA polymerase II elongation.
Lee J et al. Nat Commun 2017 Feb 20;8:14527
PMID:14527422 - BTB/POZ domain proteins are putative substrate adaptors for cullin 3 ubiquitin ligases.
Geyer R et al. Mol Cell 2003 Sep;12(3):783-90
PMID:26771498 - A Proteome-wide Fission Yeast Interactome Reveals Network Evolution Principles from Yeasts to Human.
Vo TV et al. Cell 2016 Jan 14;164(1-2):310-323
PMID:28572513 - Domain alternation and active site remodeling are conserved structural features of ubiquitin E1.
Lv Z et al. J Biol Chem 2017 Jul 21;292(29):12089-12099
PMID:21389117 - Mes1 controls the meiosis I to meiosis II transition by distinctly regulating the anaphase-promoting complex/cyclosome coactivators Fzr1/Mfr1 and Slp1 in fission yeast.
Kimata Y et al. Mol Biol Cell 2011 May;22(9):1486-94
PMID:28162934 - S. pombe Uba1-Ubc15 Structure Reveals a Novel Regulatory Mechanism of Ubiquitin E2 Activity.
Lv Z et al. Mol Cell 2017 Feb 16;65(4):699-714.e6
PMID:23760507 - Subunit architecture of the Golgi Dsc E3 ligase required for sterol regulatory element-binding protein (SREBP) cleavage in fission yeast.
Lloyd SJ et al. J Biol Chem 2013 Jul 19;288(29):21043-21054
PMID:7698660 - Schizosaccharomyces pombe and Candida albicans cDNA homologues of the Saccharomyces cerevisiae UBC4 gene.
Damagnez V et al. Gene 1995 Mar 21;155(1):137-8
PMID:16823372 - ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe.
Matsuyama A et al. Nat Biotechnol 2006 Jul;24(7):841-7
PMID:30355493 - Expanded Interactome of the Intrinsically Disordered Protein Dss1.
Schenstrøm SM et al. Cell Rep 2018 Oct 23;25(4):862-870
PMID:35075549 - Schizosaccharomyces pombe Fzo1 is subjected to the ubiquitin-proteasome-mediated degradation during the stationary phase.
Ahmad F et al. Int Microbiol 2022 May;25(2):397-404
PMID:10628977 - Isolation and characterization of Nrf1p, a novel negative regulator of the Cdc42p GTPase in Schizosaccharomyces pombe.
Murray JM et al. Genetics 2000 Jan;154(1):155-65
PMID:21511999 - Comparative functional genomics of the fission yeasts.
Rhind N et al. Science 2011 May 20;332(6032):930-6