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protein coding gene - mti2 (SPBC1271.15c) - mitochondrial translation initiation factor IF-2Mt/Mti2

Gene summary

Standard name
mti2
Systematic ID
SPBC1271.15c
Product
mitochondrial translation initiation factor IF-2Mt/Mti2
Organism
Schizosaccharomyces pombe (fission yeast)
UniProt ID
O59683
ORFeome ID
46/46D10
Characterisation status
biological role published
Feature type
mRNA gene
Genomic location
chromosome II: 334066..336676 forward strand

Annotation

GO biological process

GO:0070124 - mitochondrial translational initiation

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GO cellular component

GO:0005759 - mitochondrial matrix

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GO:0005739 - mitochondrion

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GO molecular function

GO:0005525 - GTP binding

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GO:0003924 - GTPase activity

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GO:0097177 - mitochondrial ribosome binding

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GO:0030674 - protein-macromolecule adaptor activity

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GO:0003723 - RNA binding

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GO:0003743 - translation initiation factor activity

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Multi-locus phenotype

FYPO:0001934 - abolished cell population growth on glycerol carbon source

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Genotypes:

FYPO:0001407 - decreased cell population growth on glucose carbon source

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Genotypes:

FYPO:0000087 - sensitive to hydrogen peroxide

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Genotypes:

Protein sequence feature

SO:0001808 - mitochondrial_targeting_signal

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Quantitative gene expression

PBO:0006310 - protein level

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PBO:0011963 - RNA level

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Single locus phenotype

FYPO:0008434 - abnormal mitochondrial ribosome assembly

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Genotypes:

FYPO:0001934 - abolished cell population growth on glycerol carbon source

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Genotypes:

FYPO:0000684 - decreased cell population growth on glycerol carbon source

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Genotypes:

FYPO:0009099 - decreased cell population growth on mannitol carbon source

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Genotypes:

FYPO:0003769 - decreased cellular mtDNA level

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Genotypes:

FYPO:0000708 - decreased mating efficiency

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Genotypes:

FYPO:0007122 - decreased mitochondrial respiratory chain complex assembly

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Genotypes:

FYPO:0002056 - decreased mitochondrial translation

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Genotypes:

FYPO:0001324 - decreased protein level during vegetative growth

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Genotypes:

FYPO:0007525 - decreased ribosome binding

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Genotypes:

FYPO:0001117 - decreased RNA level during vegetative growth

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Genotypes:

FYPO:0005261 - increased cell population growth on galactose carbon source

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Genotypes:

FYPO:0009093 - increased cell population growth on lysine and serine nitrogen source

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Genotypes:

FYPO:0009028 - increased cell population growth on proline nitrogen source

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Genotypes:

FYPO:0009074 - increased cell population growth on serine nitrogen source

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Genotypes:

FYPO:0002111 - inviable tapered vegetative cell

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Genotypes:

FYPO:0000245 - loss of viability in stationary phase

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Genotypes:

FYPO:0001317 - normal RNA level during vegetative growth

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Genotypes:

FYPO:0001357 - normal vegetative cell population growth

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Genotypes:

FYPO:0009038 - resistance to egtazic acid

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Genotypes:

FYPO:0005266 - resistance to sodium dodecyl sulfate

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Genotypes:

FYPO:0009067 - sensitive to amorolfine

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Genotypes:

FYPO:0001501 - sensitive to brefeldin A

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Genotypes:

FYPO:0000096 - sensitive to cadmium

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Genotypes:

FYPO:0000097 - sensitive to caffeine during vegetative growth

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Genotypes:

FYPO:0000799 - sensitive to diamide

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Genotypes:

FYPO:0000842 - sensitive to ethanol during vegetative growth

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Genotypes:

FYPO:0007928 - sensitive to ethylenediaminetetraacetic acid

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Genotypes:

FYPO:0000785 - sensitive to formamide

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Genotypes:

FYPO:0000087 - sensitive to hydrogen peroxide

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Genotypes:

FYPO:0000088 - sensitive to hydroxyurea

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Genotypes:

FYPO:0005825 - sensitive to iron

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Genotypes:

FYPO:0001719 - sensitive to lithium

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Genotypes:

FYPO:0009086 - sensitive to lithium chloride and sodium dodecyl sulfate

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Genotypes:

FYPO:0006836 - sensitive to magnesium chloride

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Genotypes:

FYPO:0009088 - sensitive to magnesium chloride and sodium dodecyl sulfate

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Genotypes:

FYPO:0000089 - sensitive to methyl methanesulfonate

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Genotypes:

FYPO:0001214 - sensitive to potassium chloride

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Genotypes:

FYPO:0007924 - sensitive to potassium chloride and sodium dodecyl sulfate

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Genotypes:

FYPO:0002328 - sensitive to terbinafine

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Genotypes:

FYPO:0000797 - sensitive to tert-butyl hydroperoxide

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Genotypes:

FYPO:0003656 - sensitive to vanadate

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Genotypes:

FYPO:0009064 - sensitive to X-rays and rapamycin during vegetative growth.

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Genotypes:

FYPO:0002060 - viable vegetative cell population

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Genotypes:

FYPO:0002177 - viable vegetative cell with normal cell morphology

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Genotypes:

Taxonomic conservation

PBO:0011067 - conserved in bacteria

PBO:0011065 - conserved in eukaryotes

PBO:0011064 - conserved in fungi

PBO:0011069 - conserved in metazoa

PBO:0011070 - conserved in vertebrates

PBO:0006222 - predominantly single copy (one to one)

Protein features

IDNameInterPro nameDB name
PF04760IF2_NIF2_NPFAM
PF00009GTP_EFTUT_Tr_GTP-bd_domPFAM
PF11987IF-2TIF_IF2_dom3PFAM
PF22042EF-G_D2EF-G-like_DIIPFAM
cd03702IF2_mtIF2_IIIF2_IICDD
cd03692mtIF2_IVcCDD
cd01887IF2_eIF5BCDD
PS01176IF2TF_IF2_bacterial-likePROSITE_PATTERNS
PS51722G_TR_2T_Tr_GTP-bd_domPROSITE_PROFILES
G3DSA:2.40.30.10:FF:000126FUNFAM
G3DSA:3.40.50.300:FF:000019FUNFAM
G3DSA:3.40.50.10050:FF:000001FUNFAM
G3DSA:2.40.30.10:FF:000008FUNFAM
SSF50447Translation proteinsTransl_B-barrel_sfSUPERFAMILY
SSF52540P-loop containing nucleoside triphosphate hydrolasesP-loop_NTPaseSUPERFAMILY
SSF52156Initiation factor IF2/eIF5b, domain 3TIF_IF2_dom3_sfSUPERFAMILY
G3DSA:3.40.50.10050TIF_IF2_dom3_sfGENE3D
G3DSA:3.40.50.300P-loop_NTPaseGENE3D
G3DSA:2.40.30.10Translation factorsGENE3D
PTHR43381TRANSLATION INITIATION FACTOR IF-2-RELATEDTIF_IF2PANTHER
TIGR00231small_GTPSmall_GTP-bdNCBIFAM
TIGR00487IF-2TF_IF2_bacterial-likeNCBIFAM

Orthologs

References / Literature

PMID:12618370 - Schizosaccharomyces pombe essential genes: a pilot study.
Decottignies A et al. Genome Res 2003 Mar;13(3):399-406
PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.
Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623
PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.
Hayles J et al. Open Biol 2013 May 22;3(5):130053
PMID:36408920 - UniProt: the Universal Protein Knowledgebase in 2023.
UniProt Consortium Nucleic Acids Res 2023 Jan 06;51(D1):D523-D531
GO_REF:0000024 - Manual transfer of experimentally-verified manual GO annotation data to orthologs by curator judgment of sequence similarity.
PMID:37787768 - Broad functional profiling of fission yeast proteins using phenomics and machine learning.
Rodríguez-López M et al. Elife 2023 Oct 03;12
PMID:21511999 - Comparative functional genomics of the fission yeasts.
Rhind N et al. Science 2011 May 20;332(6032):930-6
PMID:24763107 - Absolute proteome and phosphoproteome dynamics during the cell cycle of Schizosaccharomyces pombe (Fission Yeast).
Carpy A et al. Mol Cell Proteomics 2014 Aug;13(8):1925-36
PMID:28410370 - A systematic screen for morphological abnormalities during fission yeast sexual reproduction identifies a mechanism of actin aster formation for cell fusion.
Dudin O et al. PLoS Genet 2017 Apr;13(4):e1006721
PMID:34119521 - Schizosaccharomyces pombe Ppr10 and Mpa1 together mediate mitochondrial translational initiation.
Luo Y et al. J Biol Chem 2021 Jul;297(1):100869
GO_REF:0000002 - Comments
PMID:16823372 - ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe.
Matsuyama A et al. Nat Biotechnol 2006 Jul;24(7):841-7
PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.
Marguerat S et al. Cell 2012 Oct 26;151(3):671-83
PMID:31350787 - Schizosaccharomyces pombe Mti2 and Mti3 act in conjunction during mitochondrial translation initiation.
Luo Y et al. FEBS J 2019 Nov;286(22):4542-4553
PMID:28334955 - The Schizosaccharomyces pombe PPR protein Ppr10 associates with a novel protein Mpa1 and acts as a mitochondrial translational activator.
Wang Y et al. Nucleic Acids Res 2017 Apr 07;45(6):3323-3340
PMID:28357272 - A central role for TOR signalling in a yeast model for juvenile CLN3 disease.
Bond ME et al. Microb Cell 2015 Nov 11;2(12):466-480
PMID:34250083 - Barcode sequencing and a high-throughput assay for chronological lifespan uncover ageing-associated genes in fission yeast.
Romila CA et al. Microb Cell 2021 Jul 05;8(7):146-160
PMID:40427588 - The Insertion Domain of Mti2 Facilitates the Association of Mitochondrial Initiation Factors with Mitoribosomes in Schizosaccharomyces pombe .
Luo Y et al. Biomolecules 2025 May 10;15(5)